A highly sensitive fluorescent probe for ozone based on coumarin-benzothiazole derivative

AbstractOzone is widely used in daily life, but studies have shown that O3 can damage human trachea and lungs, leading to diseases such as asthma, emphysema, and bronchitis. Therefore, it is of great significance to develop a simple and efficient detection method for monitoring O3 in living cells. In this study, 3-(but-3-en-1-yl)-2-(7-(but-3-en-1-yloxy)-2-oxo-2H-chromen-3-yl)benzo[d]thiazol-3-ium (BCT) as a new type of water-soluble fluorescent probe was synthesized by substitution reaction of 4-bromo-1-butene and hydroxycoumarin-benzothiazole derivatives, which can specifically detect ozone in aqueous solution. The interaction of ozone on the probe can be completed within 20 min, the fluorescence intensity is significantly enhanced, and it has the advantages of high sensitivity (detection limit LOD = 43 nM). The influence of pH on the fluorescent performance of BCT shows that the probe with super stability under weak alkali and acidic environment, which provides the necessary conditions for its detection of ozone in physiological system detection. Therefore, BCT is expected to become an effective tool for detecting ozone in cellular organisms.

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High-sensitivity detection of gold labels by high-angle dark-field STEM imaging

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162028 ◽

1990 ◽

Vol 48 (3) ◽

pp. 892-893 ◽

Cited By ~ 1

Author(s):

Max T. Otten

Keyword(s):

High Sensitivity ◽

Dark Field ◽

Bright Field ◽

Backscattered Electron Imaging ◽

Backscattered Electrons ◽

Average Atomic Number ◽

Highly Sensitive ◽

Backscattered Electron ◽

Electron Imaging ◽

High Sensitivity Detection

Labelling of antibodies with small gold probes is a highly sensitive technique for detecting specific molecules in biological tissue. Larger gold probes are usually well visible in TEM or STEM Bright-Field images of unstained specimens. In stained specimens, however, the contrast of the stain is frequently the same as that of the gold labels, making it virtually impossible to identify the labels, especially when smaller gold labels are used to increase the sensitivity of the immunolabelling technique. TEM or STEM Dark-Field images fare no better (Figs. 1a and 2a), again because of the absence of a clear contrast difference between gold labels and stain.Potentially much more useful is backscattered-electron imaging, since this will show differences in average atomic number which are sufficiently large between the metallic gold and the stains normally used. However, for the thin specimens and at high accelerating voltages of the STEM, the yield of backscattered electrons is very small, resulting in a very weak signal. Consequently, the backscattered-electron signal is often too noisy for detecting small labels, even for large spot sizes.

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Highly sensitive, stable, and precise detection of dopamine with carbon dots/tyrosinase hybrid as fluorescent probe

RSC Advances ◽

10.1039/c4ra06163b ◽

2014 ◽

Vol 4 (87) ◽

pp. 46437-46443 ◽

Cited By ~ 23

Author(s):

Hao Li ◽

Juan Liu ◽

Manman Yang ◽

Weiqian Kong ◽

Hui Huang ◽

...

Keyword(s):

Fluorescent Probe ◽

Carbon Dots ◽

Low Cost ◽

High Sensitivity ◽

Highly Sensitive

The carbon dots/tyrosinase hybrid as a low-cost fluorescent probe for the detection of dopamine exhibits high sensitivity, stability, and precision.

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A novel NIR fluorescent probe for highly sensitive detection of HNO and its application in bioimaging

New Journal of Chemistry ◽

10.1039/d1nj04015d ◽

2021 ◽

Author(s):

shenwei he ◽

Jianming Zhu ◽

peiyao xie ◽

Jianfei Liu ◽

Di Zhang ◽

...

Keyword(s):

Fluorescent Probe ◽

Reactive Nitrogen Species ◽

Physiological Process ◽

Sensitive Detection ◽

Detection Methods ◽

Reactive Nitrogen ◽

Nitrogen Species ◽

Efficient Detection ◽

Highly Sensitive ◽

Highly Sensitive Detection

Nitroxyl (HNO) as an important reactive nitrogen species (RNS), which plays an important role in multiple physiological process. Therefore, it is urgent to exploit reliable and efficient detection methods for...

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Highly sensitive control of transcriptional activity by factor heterodimerization

Biochemical Journal ◽

10.1042/bj3010009 ◽

1994 ◽

Vol 301 (1) ◽

pp. 9-12 ◽

Cited By ~ 4

Author(s):

S Swillens ◽

I Pirson

Keyword(s):

Transcription Factors ◽

Transcriptional Activity ◽

Molecular Mechanisms ◽

High Sensitivity ◽

Biochemical Control ◽

Cellular Functions ◽

Effector Molecules ◽

Highly Sensitive ◽

New Type ◽

Very High

Several molecular mechanisms have been proposed to explain highly sensitive controls of cellular functions by effector molecules. Here we study an equilibrium model describing the regulation of transcriptional activity through the heterodimerization of transcription factors. We demonstrate that this model involves a new type of biochemical control which accounts for a very high sensitivity.

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A water soluble, highly sensitive and selective fluorescent probe for Al3+ ions and its application in live cell imaging

Journal of Photochemistry and Photobiology A Chemistry ◽

10.1016/j.jphotochem.2017.08.055 ◽

2017 ◽

Vol 348 ◽

pp. 246-254 ◽

Cited By ~ 18

Author(s):

Shraddha Rani Gupta ◽

Priya Singh ◽

Biplob Koch ◽

Vinod P. Singh

Keyword(s):

Fluorescent Probe ◽

Live Cell Imaging ◽

Cell Imaging ◽

Live Cell ◽

Water Soluble ◽

Highly Sensitive

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A confined carbon dot-based self-calibrated fluorescence probe for visible and high-sensitivity moisture readouts

Journal of Physics D Applied Physics ◽

10.1088/1361-6463/ac3e8f ◽

2021 ◽

Author(s):

Zhong-Zheng Ding ◽

Guang-Song Zheng ◽

Qing Lou ◽

Jiang-Fan Han ◽

Meng-Yuan Wu ◽

...

Keyword(s):

High Performance ◽

Potential Role ◽

Fluorescence Probe ◽

Color Change ◽

High Sensitivity ◽

Confinement Effect ◽

Detection Range ◽

Fluorescence Color ◽

Highly Sensitive ◽

New Type

Abstract Excellent luminescent materials are essential for high-performance fluorescent nanosensors. Here, a new-type self-calibrated humidity sensor has been established through monitoring the fluorescent color change of carbon dots (CDs) confined in sodium hydroxide (CDs@NaOH). The CDs are prepared by a facile and rapid microwave assisted heating method using citric acid, urea, and NaOH as precursors. The confinement effect from NaOH has reduced the nonradiative transition and suppressed the aggregation-induced quenching of the CDs in solid. Compared with other sensors based on CD fluorescent visualization, the sensor has good linearity and wide humidity detection range from 6.9% to 95.4%. With the increased relative humidity, the fluorescence color of the sensor change from green to blue. The proposed sensing mechanism is due to the breaking and reforming of hydrogen bonds and proton transfer occurring at the CD-NaOH matrix interfaces. This finding suggests a potential role for the spatial confinement effect and may provide an avenue for developing highly sensitive humidity readouts.

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A cationic quantum dot-based ratiometric fluorescent probe to visually detect berberine hydrochloride in human blood serums

Journal of Analytical Science & Technology ◽

10.1186/s40543-021-00261-x ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Mingyang Liu ◽

Xuejun Du ◽

Ke Xu ◽

Binwei Yan ◽

Zaibi Fan ◽

...

Keyword(s):

Fluorescent Probe ◽

Human Blood ◽

Cetylpyridinium Chloride ◽

High Sensitivity ◽

Water Soluble ◽

Detection Methods ◽

Detection Range ◽

Berberine Hydrochloride ◽

Relative Standard ◽

Ratiometric Fluorescent Probe

AbstractBerberine hydrochloride (BH) is an isoquinoline alkaloid normally used as drug to treat diseases. Compared with the traditional detection methods, the carbon quantum dots (CQDs) have better selectivity, high sensitivity, easy operation, and is inexpensive which could be widely utilized as fluorescent nanoprobes to detect various compounds quantificationally. And ratiometric fluorescent sensors conspicuously increase sensitivity and precision detection and improve quantification. In this work, we use water-soluble and fluorescent cationic carbon dots cetylpyridinium chloride monohydrate (CPC)-CQDs to connect with pinacyanol chloride (PC) and sodium tetraphenylborate (ST) as the ratiometric fluorescent probe to detect BH. The ratiometric fluorescent probe has high sensitivity towards alkaloids and metal ions, photochemical stability (60 min), and pH stability (from 6.0 to 8.0), with the detection range from 0 to 200 μM, and limit was as low as 57.35 nM. The accuracy of the method was verified by spiked recovery experiment in different human blood serums which were drawn from healthy adult volunteers to explore the practicability. The recoveries were in the range 94.34 to 105.48% with relative standard deviations (RSD) of 0.80 to 2.92%. In addition, we could observe that the fluorescence was gradually darkened, and the color turned yellow to realize the visual detection. It is expected that this work would open up a new strategy for detecting BH in the environment and human blood serums.

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A novel water soluble multifunctional fluorescent probe for highly sensitive and ultrafast detection of anionic surfactants and wash free imaging of Gram-positive bacteria strains

Dyes and Pigments ◽

10.1016/j.dyepig.2017.05.006 ◽

2017 ◽

Vol 143 ◽

pp. 436-443 ◽

Cited By ~ 17

Author(s):

Tang Gao ◽

Xiaozheng Cao ◽

Jie Dong ◽

Yi Liu ◽

Wuwu Lv ◽

...

Keyword(s):

Fluorescent Probe ◽

Anionic Surfactants ◽

Water Soluble ◽

Gram Positive ◽

Gram Positive Bacteria ◽

Highly Sensitive ◽

Ultrafast Detection

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DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs

Sensors ◽

10.3390/s20072017 ◽

2020 ◽

Vol 20 (7) ◽

pp. 2017

Author(s):

Chen Liu ◽

Jialun Han ◽

Lujian Zhou ◽

Jingjing Zhang ◽

Jie Du

Keyword(s):

Signal Amplification ◽

Tumor Suppressor Genes ◽

Low Cost ◽

Rolling Circle Amplification ◽

High Sensitivity ◽

Rolling Circle ◽

Highly Sensitive ◽

And Control ◽

High Sensitivity Detection

MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L−1 level hence affording a simple, highly sensitive, and selective low cost detection platform.

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