scholarly journals Thermoresponsive-polymer-based materials for temperature-modulated bioanalysis and bioseparations

2016 ◽  
Vol 4 (39) ◽  
pp. 6381-6397 ◽  
Author(s):  
Kenichi Nagase ◽  
Teruo Okano
Keyword(s):  
Bioactive Compounds ◽  
Thermoresponsive Polymer ◽  
Thermoresponsive Polymers ◽  
Cell Separations

In this review, bioseparations using thermoresponsive polymers are summarized. Thermoresponsive chromatography for separating bioactive compounds and proteins, and cell separations using thermoresponsive polymers and their properties are reviewed.

scholarly journals Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kenichi Nagase ◽  
Yuta Umemoto ◽  
Hideko Kanazawa
Keyword(s):  
Pore Diameter ◽  
Packed Column ◽  
Packed Columns ◽  
Butyl Methacrylate ◽  
Thermoresponsive Polymer ◽  
Thermoresponsive Polymers ◽  
Column Temperature ◽  
Pore Sizes ◽  
Temperature Responsive ◽  
Elution Behavior

AbstractTemperature-responsive chromatography using thermoresponsive polymers is innovative and can control analyte retention via column temperature. Analyte elution behavior in this type of chromatography depends on the modified thermoresponsive polymer and the structure of the base materials. In the present study, we examine the effect of the pore diameter of silica beads on analyte elution behavior in temperature-responsive chromatography. Poly(N-isopropylacrylamide-co-n-butyl methacrylate) hydrogel was applied to beads of various pore sizes: 7, 12, and 30 nm. Almost the same amount of copolymer hydrogel was applied to all beads, indicating that the efficiency of copolymer modification was independent of pore size. Analyte retention on prepared beads in a packed column was observed using steroids, benzodiazepines, and barbiturates as analytes. Analyte retention times increased with temperature on packed columns of 12- and 30-nm beads, whereas the column packed with 7-nm beads exhibited decreased retention times with increasing temperature. The difference in analyte elution behavior among the various pore sizes was attributed to analyte diffusion into the bead pores. These results demonstrate that bead pore diameter determines temperature-dependent elution behavior.

Effects of Graft Densities and Chain Lengths on Separation of Bioactive Compounds by Nanolayered Thermoresponsive Polymer Brush Surfaces

Langmuir ◽  
2008 ◽  
Vol 24 (2) ◽  
pp. 511-517 ◽  
Author(s):  
Kenichi Nagase ◽  
Jun Kobayashi ◽  
Akihiko Kikuchi ◽  
Yoshikatsu Akiyama ◽  
Hideko Kanazawa ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Polymer Brush ◽  
Thermoresponsive Polymer ◽  
Chain Lengths

scholarly journals Novel thermoresponsive polymer outperforming Pluronic® F127

2020 ◽  
Author(s):  
Anna Constantinou ◽  
Valeria Nele ◽  
James Doutch ◽  
Roman Moiseev ◽  
Vitaliy Khutoryanskiy ◽  
...  
Keyword(s):  
Self Assembly ◽  
Biomedical Applications ◽  
Ex Vivo ◽  
Structural Characteristics ◽  
Thermoresponsive Polymer ◽  
Scanning Calorimetry ◽  
Thermoresponsive Polymers ◽  
Crosslinked Networks ◽  
Gelation Concentration ◽  
Physically Crosslinked

Abstract Thermoresponsive polymers featuring the appropriate combination of structural characteristics, i.e. architecture, composition, and molar mass (MM), can form physically crosslinked networks in a solvent upon changes in temperature. This fascinating class of polymers finds utility in various sectors such as formulation science and tissue engineering. Here, we report a novel thermoresponsive triblock terpolymer which out-performs the most commonly used and commercially available thermoresponsive polymer, Poloxamer P407 (also known as Pluronic® F127) in terms of gelation concentration. Specifically, the in-house synthesised polymer forms gels at lower concentrations that is an advantage in biomedical applications. To elucidate the differences in their macroscale gelling behaviour, we investigate their micellization via differential scanning calorimetry, and their nanoscale self-assembly behaviour in detail by means of small-angle neutron scattering by simultaneously recording their rheological properties (Rheo-SANS). Two different gelation mechanisms for the two polymers are revealed and proposed. Ex vivo gelation study upon intracameral injections demonstrated excellent potential for its application to improve drug residence in the eye.

Interaction of bioactive compounds on capillary inner surfaces bearing a dense thermoresponsive polymer brush

2016 ◽  
Vol 28 (10-12) ◽  
pp. 900-912 ◽  
Author(s):  
Takuya Koriyama ◽  
Yosuke Takayama ◽  
Chiho Hisatsune ◽  
Taka-Aki Asoh ◽  
Akihiko Kikuchi
Keyword(s):  
Bioactive Compounds ◽  
Polymer Brush ◽  
Thermoresponsive Polymer

Thermoresponsive polymer-modified microfibers for cell separations

2017 ◽  
Vol 53 ◽  
pp. 81-92 ◽  
Author(s):  
Kenichi Nagase ◽  
Yoichi Sakurada ◽  
Satoru Onizuka ◽  
Takanori Iwata ◽  
Masayuki Yamato ◽  
...  
Keyword(s):  
Thermoresponsive Polymer ◽  
Cell Separations

scholarly journals Thermoresponsive Polymers: Thermoresponsive Polymer Induced Sweating Surfaces as an Efficient Way to Passively Cool Buildings (Adv. Mater. 39/2012)

2012 ◽  
Vol 24 (39) ◽  
pp. 5277-5277 ◽  
Author(s):  
A. C. C. Rotzetter ◽  
C. M. Schumacher ◽  
S. B. Bubenhofer ◽  
R. N. Grass ◽  
L. C. Gerber ◽  
...  
Keyword(s):  
Thermoresponsive Polymer ◽  
Thermoresponsive Polymers

scholarly journals Cross-linker effect on solute adsorption in swollen thermoresponsive polymer networks

2019 ◽  
Vol 21 (12) ◽  
pp. 6588-6599 ◽  
Author(s):  
Sebastian Milster ◽  
Richard Chudoba ◽  
Matej Kanduč ◽  
Joachim Dzubiella
Keyword(s):  
Molecular Dynamics ◽  
Polymer Networks ◽  
Cross Link ◽  
Thermoresponsive Polymer ◽  
Thermoresponsive Polymers ◽  
Cross Linker ◽  
Solute Adsorption ◽  
The Cross ◽  
Linker Effect

Molecular dynamics study on the solute adsorption to thermoresponsive polymers estimating the cross-link impact on particle partitioning in swollen hydrogels.

Thermoresponsive Polymer Brush on Monolithic-Silica-Rod for the High-Speed Separation of Bioactive Compounds

Langmuir ◽  
2011 ◽  
Vol 27 (17) ◽  
pp. 10830-10839 ◽  
Author(s):  
Kenichi Nagase ◽  
Jun Kobayashi ◽  
Akihiko Kikuchi ◽  
Yoshikatsu Akiyama ◽  
Hideko Kanazawa ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Speed ◽  
Polymer Brush ◽  
Thermoresponsive Polymer ◽  
Monolithic Silica
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