scholarly journals Injectable dynamic covalent hydrogels of boronic acid polymers cross-linked by bioactive plant-derived polyphenols

2018 ◽  
Vol 6 (9) ◽  
pp. 2487-2495 ◽  
Author(s):  
Zhuojun Huang ◽  
Peyman Delparastan ◽  
Patrick Burch ◽  
Jing Cheng ◽  
Yi Cao ◽  
...  
Keyword(s):  
Boronic Acid ◽  
Polyphenolic Compounds ◽  
Functionalized Polymers ◽  
Covalent Bonds ◽  
Cross Linker ◽  
And Function

Wisdom from nature: Plant-derived polyphenolic compounds can crosslink boronic acid functionalized polymers through formation of dynamic covalent bonds and function as both cross-linker and bioactive cargo.

scholarly journals The Modern Western Diet Rich in Advanced Glycation End-Products (AGEs): An Overview of Its Impact on Obesity and Early Progression of Renal Pathology

Nutrients ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1748 ◽  
Author(s):  
Arianna Bettiga ◽  
Francesco Fiorio ◽  
Federico Di Marco ◽  
Francesco Trevisani ◽  
Annalisa Romani ◽  
...  
Keyword(s):  
Chronic Diseases ◽  
Advanced Glycation End Products ◽  
Cell Function ◽  
Advanced Glycation ◽  
Covalent Bonds ◽  
Physiological Processes ◽  
Glycation End Products ◽  
End Products ◽  
And Function

Advanced glycation end-products (AGEs) are an assorted group of molecules formed through covalent bonds between a reduced sugar and a free amino group of proteins, lipids, and nucleic acids. Glycation alters their structure and function, leading to impaired cell function. They can be originated by physiological processes, when not counterbalanced by detoxification mechanisms, or derive from exogenous sources such as food, cigarette smoke, and air pollution. Their accumulation increases inflammation and oxidative stress through the activation of various mechanisms mainly triggered by binding to their receptors (RAGE). So far, the pathogenic role of AGEs has been evidenced in inflammatory and chronic diseases such as chronic kidney disease, cardiovascular disease, and diabetic nephropathy. This review focuses on the AGE-induced kidney damage, by describing the molecular players involved and investigating its link to the excess of body weight and visceral fat, hallmarks of obesity. Research regarding interventions to reduce AGE accumulation has been of great interest and a nutraceutical approach that would help fighting chronic diseases could be a very useful tool for patients’ everyday lives.

scholarly journals Structure-function studies of CrmK, an oxidase involved in Caerulomycin A biosynthesis

2014 ◽  
Vol 70 (a1) ◽  
pp. C1669-C1669
Author(s):  
Marie-Ève Picard ◽  
Julie Barma ◽  
Yiguang Zhu ◽  
Xavier Murphy Després ◽  
Jean-Baptiste Duvignaud ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Binding Pocket ◽  
Antimicrobial Activities ◽  
Active Site Residues ◽  
Covalent Bonds ◽  
Protein Residues ◽  
Function Relationship ◽  
Structure And Function Relationship ◽  
And Function ◽  
Relationship Of

Caerulomycin A (CRM A) is an immunosupressive agent that has a unique 2,2'-bipyridine core structure. Isolated from a marine-derived Actinoalloteichus cyanogriseus, this natural product exhibits antifungal, anti-amoebic, antitumor, and antimicrobial activities. Its biosynthetic pathway consists of more than 20 enzymes, at least seven of which are putatively involved in post-PKS/NRPS modifications of the scaffold. Among these, CrmK is a flavin-dependent oxidase. We have determined the crystal structure of CrmK bound to its flavin adenin dinucleotide (FAD) cofactor at 1.9 Å resolution. FAD linkage to CrmK is observed via two covalent bonds with protein residues His64 and Cys124. This crystal structure, combined with the activity analysis of both wild-type CrmK and a series of mutants, has revealed the role of active site residues lining the substrate and FAD binding pocket. Our studies add additional molecular insights into the structure and function relationship of the bicovalently flavinylated oxidases.

scholarly journals A polyrotaxane gel using boronic acid-appended γ-cyclodextrin as a hybrid cross-linker

2017 ◽  
Vol 89 (3-4) ◽  
pp. 281-288 ◽  
Author(s):  
Wataru Uchida ◽  
Maiki Yoshikawa ◽  
Tomohiro Seki ◽  
Ryotaro Miki ◽  
Toshinobu Seki ◽  
...  
Keyword(s):  
Boronic Acid ◽  
Cross Linker ◽  
Hybrid Cross

scholarly journals Impact of Two Novel Mutations on the Structure and Function of Human Myeloperoxidase

2007 ◽  
Vol 282 (38) ◽  
pp. 27994-28003 ◽  
Author(s):  
Melissa Goedken ◽  
Sally McCormick ◽  
Kevin G. Leidal ◽  
Kazuo Suzuki ◽  
Yosuke Kameoka ◽  
...  
Keyword(s):  
Aminolevulinic Acid ◽  
Soret Band ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Covalent Bonds ◽  
Human Embryonic Kidney Cells ◽  
Heme Pocket ◽  
Japanese Adults ◽  
And Function ◽  
Structural Aspects

The heme protein myeloperoxidase (MPO) contributes critically to O2-dependent neutrophil antimicrobial activity. Two Japanese adults were identified with inherited MPO deficiency because of mutations at Arg-499 or Gly-501, conserved residues near the proximal histidine in the heme pocket. Because of the proximity of these residues to a critical histidine in the heme pocket, we examined the biosynthesis, function, and spectral properties of the peroxidase stably expressed in human embryonic kidney cells. Biosynthesis of normal MPO by human embryonic kidney cells faithfully mirrored events previously identified in cells expressing endogenous MPO. Mutant apopro-MPO was 90 kDa and interacted normally with the molecular chaperones ERp57, calreticulin, and calnexin in the endoplasmic reticulum. However, mutant precursors were not proteolytically processed into subunits of MPO, although secretion of the unprocessed precursors occurred normally. Although δ-[14C]aminolevulinic acid incorporation demonstrated formation of pro-MPO in both mutants, neither protein was enzymatically active. The Soret band for each mutant was shifted from the normal 430 to ∼412 nm, confirming that heme was incorporated but suggesting that the number of covalent bonds or other structural aspects of the heme pocket were disrupted by the mutations. These studies demonstrate that despite heme incorporation, mutations in the heme environs compromised the oxidizing potential of MPO.

Energy Dissipation and Mechanoresponsive Color Evaluation of a Poly(n-hexyl Methacrylate) Soft Material Enhanced by a Mechanochromic Cross-Linker with Dynamic Covalent Bonds

2020 ◽  
Vol 53 (21) ◽  
pp. 9313-9324
Author(s):  
Yuchen Mao ◽  
Yuto Kubota ◽  
Takashi Kurose ◽  
Akira Ishigami ◽  
Kota Seshimo ◽  
...  
Keyword(s):  
Energy Dissipation ◽  
Soft Material ◽  
Covalent Bonds ◽  
Cross Linker ◽  
Hexyl Methacrylate

Let there be light: how to use photoswitchable cross-linker to reprogram proteins

2017 ◽  
Vol 45 (3) ◽  
pp. 831-837 ◽  
Author(s):  
Daniel Hoersch
Keyword(s):  
Rational Design ◽  
Design Strategies ◽  
Molecular Tweezers ◽  
Protein Targets ◽  
Reactive Groups ◽  
Synthetic Cells ◽  
End Distance ◽  
Cross Linker ◽  
Cellular Pathways ◽  
And Function

Azobenzene is a photo-isomerizing molecule whose end-to-end distance changes upon external illumination. When combined with site-specific reactive groups, it can be used as molecular tweezers to remote-control the structure and function of protein targets. The present study gives a brief overview over the rational design strategies that use an azobenzene-based photoswitchable cross-linker to engineer ON/OFF switches into functional proteins or to reprogram proteins for novel functions. The re-engineered proteins may be used as remote controls for cellular pathways, as light-gated drug delivery platforms or as light-powered machinery of synthetic cells and micro-scaled factories.

Characterization of Factor VIII Related Protein Synthesized by Human Endothelial Cell: A Study of Structure and Function

1982 ◽  
Vol 48 (02) ◽  
pp. 177-181 ◽  
Author(s):  
Vivian Chan ◽  
T K Chan
Keyword(s):  
Endothelial Cell ◽  
Factor Viii ◽  
Culture Medium ◽  
Sodium Dodecyl ◽  
Von Willebrand Disease ◽  
Human Endothelial Cell ◽  
Molecular Forms ◽  
Related Protein ◽  
Covalent Bonds ◽  
And Function

SummaryCrossed immunoelectrophoresis showed that factor VIII related protein (VIII R) synthesized in the human endothelial cell (EC-VIII R) had faster electrophoretic mobility than that secreted into the culture medium (MED-VIII R). Sodium dodecyl sulphate agarose gel electrophoresis followed by radioimmunofixation showed that EC-VIII R consisted of molecules varying from 0.26 × 106 to 3.76 × 106 daltons while MED-VIII R had molecules ranging from 0.93 × 106 to greater than 10 × 106 daltons, similar to that present in plasma. The smallest VIII R molecule present in normal plasma or spent culture medium (0.93 × 106 daltons) corresponded to a tetramer of subunits of 0.22-0.24 × 106 daltons. Only molecular forms greater than 3.76 × 106 daltons possessed ristocetin cofactor activity. Sonication (15 μ. amplitued for 30 secs) effectively broke the non-covalent bonds of the VIII R multimers resulting in smaller molecules. Thus endothelial cells in culture synthesized VIII R subunits and assembled them into the higher multimeric forms on secretion. Different types of von Willebrand disease could result from defects of either of the two processes.

Supramolecular design in 2D covalent organic frameworks

2020 ◽  
Vol 49 (5) ◽  
pp. 1344-1356 ◽  
Author(s):  
Sampath B. Alahakoon ◽  
Shashini D. Diwakara ◽  
Christina M. Thompson ◽  
Ronald A. Smaldone
Keyword(s):  
Supramolecular Chemistry ◽  
Porous Polymers ◽  
Covalent Organic Frameworks ◽  
Covalent Bonds ◽  
Crystalline Structures ◽  
Form And Function ◽  
And Function

2D covalent organic frameworks (COFs) are a class of porous polymers with crystalline structures. This tutorial review discusses how the concepts of supramolecular chemistry are used to add form and function to COFs through their non-covalent bonds.

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