Intracellular low-abundance microRNA imaging by a NIR-assisted entropy-driven DNA system

Screening results indicated that DCO-5 exhibited the highest sensitivity to viscosity and was insensitive to polarity or pH, and enables the successful detection of viscosity changes in vivo.

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A Highly Sensitive Strategy for Fluorescence Imaging of MicroRNA in Living Cells and in Vivo Based on Graphene Oxide-Enhanced Signal Molecules Quenching of Molecular Beacon

ACS Applied Materials & Interfaces ◽

10.1021/acsami.7b19284 ◽

2018 ◽

Vol 10 (8) ◽

pp. 6982-6990 ◽

Cited By ~ 39

Author(s):

Limin Yang ◽

Bo Liu ◽

Meimei Wang ◽

Jia Li ◽

Wei Pan ◽

...

Keyword(s):

Graphene Oxide ◽

Fluorescence Imaging ◽

Molecular Beacon ◽

Living Cells ◽

Signal Molecules ◽

Highly Sensitive

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A highly sensitive red-emitting probe for the detection of viscosity changes in living cells, zebrafish, and human blood samples

Journal of Materials Chemistry B ◽

10.1039/c9tb02023c ◽

2020 ◽

Vol 8 (6) ◽

pp. 1310-1315 ◽

Cited By ~ 3

Author(s):

Manlin Fu ◽

Wei Shen ◽

Yajun Chen ◽

Wenjun Yi ◽

Chunhui Cai ◽

...

Keyword(s):

Human Blood ◽

Fluorescent Probes ◽

Living Cells ◽

The State ◽

Blood Samples ◽

Highly Sensitive ◽

Human Blood Samples ◽

Viscosity Changes

Intracellular viscosity can be measured to reflect the state of living cells. Fluorescent probes are powerful tools for viscosity detection in vivo.

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A novel and effective benzo[d]thiazole-based fluorescent probe with dual recognition factors for highly sensitive and selective imaging of cysteine in vitro and in vivo

New Journal of Chemistry ◽

10.1039/c9nj03202a ◽

2019 ◽

Vol 43 (34) ◽

pp. 13463-13470 ◽

Cited By ~ 7

Author(s):

Meiqing Zhu ◽

Xiaoqin Wu ◽

Linfeng Sang ◽

Fugang Fan ◽

Lijun Wang ◽

...

Keyword(s):

Fluorescent Probe ◽

Living Cells ◽

Highly Sensitive

Double recognition groups significantly improved the selectivity of a fluorescent probe to Cys in living cells.

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Probe for simultaneous membrane and nucleus labeling in living cells and in vivo bioimaging using a two-photon absorption water-soluble Zn(ii) terpyridine complex with a reduced π-conjugation system

Chemical Science ◽

10.1039/c6sc02342h ◽

2017 ◽

Vol 8 (1) ◽

pp. 142-149 ◽

Cited By ~ 39

Author(s):

Xiaohe Tian ◽

Qiong Zhang ◽

Mingzhu Zhang ◽

Kajsa Uvdal ◽

Qin Wang ◽

...

Keyword(s):

Live Cell Imaging ◽

Cell Imaging ◽

Living Cells ◽

Water Soluble ◽

Photon Absorption ◽

Conjugation System ◽

Two Photon Absorption ◽

Two Photon ◽

System P

A two-photon absorption water-soluble Zn(ii) probe for simultaneous membrane nucleus live cell imaging and its potential for blood-brain barrier staining.

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Detecting and imaging of SO2 derivatives in living cells with zero cross-talk colorimetric mitochondria-targeted fluorescent probe

Canadian Journal of Chemistry ◽

10.1139/cjc-2019-0216 ◽

2020 ◽

Vol 98 (1) ◽

pp. 1-6

Author(s):

Dan Wu ◽

Shiqi Rong ◽

Yi Liu ◽

Fei Zheng ◽

Yankun Zhao ◽

...

Keyword(s):

High Resolution ◽

Cross Talk ◽

High Sensitivity ◽

Living Cells ◽

High Resolution Imaging ◽

Biochemical Processes ◽

Highly Sensitive ◽

Resolution Imaging ◽

Low Cytotoxicity

It is well known that excessive levels of sulfur dioxide and its derivatives are connected to diverse diseases. Therefore, developing highly sensitive probes to detect and monitor sulfite in living cells is important for the diagnosis of disease and the study of biochemical processes in vivo. In this report, two zero cross-talk ratiometric fluorescent probes were synthesized (CA-ID-MC and CA-BI-MC), which were derived from carbazole-indolenine π-conjugated system for effective detection of sulfite in living cells. Observably, CA-BI-MC exhibited the largest emission shift of 157 nm from 617 to 460 nm with the addition of various concentrations of sulfite, which is beneficial for high-resolution imaging of the sulfite. CA-BI-MC also exhibits high sensitivity and low cytotoxicity. More importantly, this probe successfully located mitochondria and sensed the sulfite in HeLa cells caused by exogenous stimulation.

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In vivo mRNA imaging based on tripartite DNA probe mediated catalyzed hairpin assembly

Chemical Communications ◽

10.1039/d0cc03596c ◽

2020 ◽

Vol 56 (62) ◽

pp. 8782-8785 ◽

Cited By ~ 1

Author(s):

Han Wu ◽

Wen-Jing Zhou ◽

Lan Liu ◽

Ze Fan ◽

Hao Tang ◽

...

Keyword(s):

Living Cells ◽

Dna Probe ◽

Catalyzed Hairpin Assembly ◽

Specific Mrna

Herein, we develop a novel tripartite DNA probe to transport phosphorothioated substrate hairpins and an aptamer for the intramolecular CHA circuit, which achieves detection of a low amount of specific mRNA in living cells and mice.

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Aptamer superstructure-based electrochemical biosensor for sensitive detection of ATP in rat brain with in vivo microdialysis

The Analyst ◽

10.1039/c8an02077a ◽

2019 ◽

Vol 144 (5) ◽

pp. 1711-1717 ◽

Cited By ~ 8

Author(s):

Yanan Jiang ◽

Wenjie Ma ◽

Wenliang Ji ◽

Huan Wei ◽

Lanqun Mao

Keyword(s):

Rat Brain ◽

Electrochemical Biosensor ◽

In Vivo Microdialysis ◽

Sensitive Detection ◽

Recognition Element ◽

System P ◽

Highly Sensitive ◽

Highly Sensitive Detection

An electrochemical biosensor with an aptamer superstructure as the recognition element is demonstrated for highly sensitive detection of ATP in the cerebral system.

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Fluorescence ratio imaging of dynamic intracellular ionic signals

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102298 ◽

1988 ◽

Vol 46 ◽

pp. 42-43

Author(s):

R. Y. Tsien ◽

A. Minta ◽

M. Poenie ◽

J.P.Y. Kao ◽

A. Harootunian

Keyword(s):

Binding Sites ◽

Living Cells ◽

Molecular Engineering ◽

Ph Indicators ◽

Highly Sensitive ◽

Fluorescence Ratio Imaging ◽

Ratio Imaging ◽

Technical Advances ◽

Indicator Dyes ◽

Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

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