Electrochemical synthesis and in vitro cytotoxicity study of copper(ii) carboxylates with different fatty acid alkyl chain lengths

Background: More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations. Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China. Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell lines MGC-803 and A549. Conclusion: The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism. Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the chromosome-damaging effects of the four MN-test-positive chemicals.

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Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.

Food & Function ◽

10.1039/d0fo02509g ◽

2021 ◽

Author(s):

Shiyi Lu ◽

Deirdre Mikkelsen ◽

Hong Yao ◽

Barbara Williams ◽

Bernadine Flanagan ◽

...

Keyword(s):

Fatty Acid ◽

Gut Microbiota ◽

Plant Cell ◽

Cell Walls ◽

Short Chain Fatty Acid ◽

Chain Fatty Acid ◽

Plant Cell Walls ◽

Human Gut ◽

In Vitro Fermentation

Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the...

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The efficacy of organic acid, medium chain fatty acid, and essential oil based broiler treatments; in vitro anti‐ Campylobacter jejuni activity and the effect of these chemical‐based treatments on broiler performance

Journal of Applied Microbiology ◽

10.1111/jam.15204 ◽

2021 ◽

Author(s):

G. Greene ◽

L. Koolman ◽

P. Whyte ◽

H. Lynch ◽

A. Coffey ◽

...

Keyword(s):

Fatty Acid ◽

Essential Oil ◽

Organic Acid ◽

Acid Medium ◽

Campylobacter Jejuni ◽

Chain Fatty Acid ◽

Medium Chain Fatty Acid ◽

Broiler Performance ◽

Medium Chain

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In Vivo Interactions with (Tissue Culture Pretreated) Dermal Sheep Collagen

MRS Proceedings ◽

10.1557/proc-252-117 ◽

1991 ◽

Vol 252 ◽

Cited By ~ 3

Author(s):

P. B. van Wachem ◽

L. H. H. Olde Damink ◽

P. J. Dijkstra ◽

J. Feijen ◽

...

Keyword(s):

Tissue Culture ◽

Cell Death ◽

Marked Reduction ◽

Giant Cells ◽

In Vitro Cytotoxicity ◽

Cell Infiltration ◽

Cytotoxic Effects ◽

Lipid Formation

ABSTRACTPretreatment in tissue culture (TC) was previously found to markedly reduce the in vitro cytotoxicity of two types of crosslinked dermal sheep collagens (DSC's). This in vivo study confirms our in vitro results, in that TC-pretreatment of crosslinked DSC's resulted in the marked reduction or elimination of cytotoxic effects, such as increased cell infiltration, a deviant neutrophil-morphology, lipid formation and cell death. TC-pretreatment affected the crosslinked state of both DSC's in a different way, which could be deduced from the differences in gelatin-formation and presence of giant cells from macrophage- or fibroblast-origin. The results are explained in view of the differences in crosslinking.

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Short-chain fatty acid transport and its effects on ion transport by rabbit cecum

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1987.253.2.g171 ◽

1987 ◽

Vol 253 (2) ◽

pp. G171-G178

Author(s):

M. Hatch

Keyword(s):

Fatty Acid ◽

Short Chain Fatty Acid ◽

Short Circuit ◽

Basolateral Membrane ◽

Chain Fatty Acid ◽

Short Chain ◽

Bathing Solution ◽

Fatty Acid Transport ◽

Unexpected Finding

Short-chain fatty acid (SCFA) metabolism and transport were examined in vitro across isolated rabbit cecal epithelia whose primary function is absorption of these solutes. This study shows that although there was some low-level metabolism of SCFAs to ketone bodies by the isolated cecum, a significantly higher oxygen consumption was sustained for a longer time period by tissues incubated in glucose-containing salines. The cecum supported a significant net secretory flux of acetate (J net Ac- = -1.13 +/- 0.13 mu eq X cm-2 X h-1) and propionate (J net Pr- = -0.61 +/- 0.14 mu eq X cm-2 X h-1). This study also shows that glucose significantly enhanced short-circuit current (Isc), tissue conductance (Gt), and sodium transport across this tissue. Neither Ac- nor Pr- enhanced net sodium flux (J net Ac-) but Pr- significantly reduced net chloride flux (J net Cl-), whereas Ac- had no effect. The increase in Isc and Gt observed in the presence of SCFAs was attributable to the presence of SCFA in the serosal bathing solution alone. To explain the latter finding and the unexpected finding of SCFA secretion, the existence of an electrogenic anion (HCO3-) secretory pathway is postulated. It is suggested that this system can accommodate SCFAs in vitro and that it is a Na+-dependent system located on the basolateral membrane of the cecal cell.

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