scholarly journals Molecular species fingerprinting and quantitative analysis of saffron (Crocus sativus L.) for quality control by MALDI mass spectrometry

RSC Advances ◽  
2018 ◽  
Vol 8 (63) ◽  
pp. 36104-36113 ◽  
Author(s):  
Donatella Aiello ◽  
Carlo Siciliano ◽  
Fabio Mazzotti ◽  
Leonardo Di Donna ◽  
Constantinos M. Athanassopoulos ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
Quantitative Analysis ◽  
Molecular Species ◽  
Reliable Method ◽  
Maldi Mass Spectrometry ◽  
Crocus Sativus ◽  
Ms Analysis ◽  
Crocus Sativus L

Herein we describe a rapid, simple, and reliable method for the quantitative analysis and molecular species fingerprinting of saffron (Crocus sativus L.) by direct MS and MS/MS analysis.

scholarly journals Proteomic identification of biomarkers in maternal plasma that predict the outcome of rescue cerclage for cervical insufficiency

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250031
Author(s):  
Kisoon Dan ◽  
Ji Eun Lee ◽  
Dohyun Han ◽  
Sun Min Kim ◽  
Subeen Hong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Multiple Reaction Monitoring ◽  
Area Under The Curve ◽  
Maternal Plasma ◽  
Tandem Mass ◽  
Cervical Insufficiency ◽  
Plasma Samples ◽  
Biomarker Panel ◽  
Ms Analysis

Objective We sought to identify plasma protein biomarkers that are predictive of the outcome of rescue cerclage in patients with cervical insufficiency. Methods This retrospective cohort study included 39 singleton pregnant women undergoing rescue cerclage for cervical insufficiency (17–25 weeks) who gave plasma samples. Three sets of pooled plasma samples from controls (cerclage success, n = 10) and cases (cerclage failure, n = 10, defined as spontaneous preterm delivery at <33 weeks) were labeled with 6-plex tandem mass tag (TMT) reagents and analyzed by liquid chromatography-tandem mass spectrometry. Differentially expressed proteins between the two groups were selected from the TMT-based quantitative analysis. Multiple reaction monitoring-mass spectrometry (MRM-MS) analysis was further used to verify the candidate proteins of interest in patients with cervical insufficiency in the final cohort (n = 39). Results From MRM-MS analysis of the 40 proteins showing statistically significant changes (P < 0.05) from the TMT-based quantitative analysis, plasma IGFBP-2, PSG4, and PGLYRP2 levels were found to be significantly increased, whereas plasma MET and LXN levels were significantly decreased in women with cerclage failure. Of these, IGFBP-2, PSG4, and LXN levels in plasma were independent of cervical dilatation. A multiple-biomarker panel was developed for the prediction of cerclage failure, using a stepwise regression procedure, which included the plasma IGFBP-2, PSG4, and LXN (area under the curve [AUC] = 0.916). The AUC for this multiple-biomarker panel was significantly greater than the AUC for any single biomarker included in the multi-biomarker model. Conclusions Proteomic analysis identified useful and independent plasma biomarkers (IGFBP-2, PSG4, and LXN; verified by MRM) that predict poor pregnancy outcome following rescue cerclage. Their combined analysis in a multi-biomarker panel significantly improved predictability.

Quality control of saffron (Crocus sativus L.) via a novel HPLC method

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
K Kuchta ◽  
HH Jin ◽  
RW Wang ◽  
HH He ◽  
L Fang ◽  
...  
Keyword(s):  
Quality Control ◽  
Hplc Method ◽  
Crocus Sativus ◽  
Crocus Sativus L

scholarly journals A Biosensor-Based Quantitative Analysis System of Major Active Ingredients in Lonicera japonica Thunb. Using UPLC-QDa and Chemometric Analysis

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1787 ◽  
Author(s):  
Lin Yang ◽  
Hai Jiang ◽  
Xudong Xing ◽  
Meiling Yan ◽  
Xinyue Guo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
Quantitative Analysis ◽  
High Performance ◽  
Inflammatory Diseases ◽  
Screening Method ◽  
Chemometric Analysis ◽  
Lonicera Japonica ◽  
Detection Range ◽  
Tnf Α

In the study, a surface plasmon resonance-based (SPR-based) competitive assay was performed to analyze different compounds’ inhibitory activity to TNF-α, an important pro-inflammatory cytokine in the pathogenesis of chronic inflammatory diseases. Moreover, the single mass spectrometry (MS) detection method was coupled with an ultra-high-performance liquid chromatography (UPLC) system for the routine quality control (QC) of a traditional Chinese medicine (TCM). The above quality control strategy was evaluated with Lonicera japonica Thunb. Analytes were firstly separated on a Waters ACQUITYTM UPLC HSS T3 column (2.1 × 50 mm; particle size = 1.8 μm) using a 0.1% formic acid gradient elution, then detected by negative ESI mass spectrometry. The limits of quantification (LOQ) for analytes reached 0.005–0.56 μg/mL. The LOD of the QDa detector was lower than that of the PDA detector, indicating its wider detection range. The QDa detector was also more suitable for the analysis of the complex matrix of TCM. The method showed excellent linearity, with regression coefficients higher than 0.9991. The average recoveries of the investigated analytes were in the range of 98.78–105.13%, with an RSD below 3.91%. The inter-day precision range (n = 3 days) was 2.51–4.54%. Compared to other detectors, this strategy could be widely applied in the quantitative analysis of TCM. In addition, the chemically latent data could be revealed using chemometric analysis. Importantly, this study provides an efficient screening method for small-molecule inhibitors targeting the TNF-α pathway.

Solid-phase extraction for picrocrocin determination in the quality control of saffron spice (Crocus sativus L.)

2009 ◽  
Vol 116 (3) ◽  
pp. 792-798 ◽  
Author(s):  
Ana María Sánchez ◽  
Manuel Carmona ◽  
Carmen Priscila del Campo ◽  
Gonzalo Luis Alonso
Keyword(s):  
Quality Control ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Crocus Sativus ◽  
Phase Extraction ◽  
Crocus Sativus L
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