Tough and tissue-adhesive polyacrylamide/collagen hydrogel with dopamine-grafted oxidized sodium alginate as crosslinker for cutaneous wound healing

Natural collagen has good biocompatibility and ability to promote tissue regeneration; however, its low flexibility and easy degradation hinder its applications in wound repair.

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Delivery of mesenchymal stem cells in a biomimetic collagen hydrogel enhances cutaneous wound healing

Journal of the American College of Surgeons ◽

10.1016/j.jamcollsurg.2010.06.241 ◽

2010 ◽

Vol 211 (3) ◽

pp. S91-S92 ◽

Cited By ~ 5

Author(s):

Kristine Rustad ◽

Victor W. Wong ◽

Michael Sorkin ◽

Jason P. Glotzbach ◽

Dean Nehama ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Cutaneous Wound Healing ◽

Cutaneous Wound ◽

Collagen Hydrogel

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Harnessing the Natural Healing Power of Colostrum: Bovine Milk‐derived Extracellular Vesicles from Colostrum Facilitating the Transition from Inflammation to Tissue Regeneration for Accelerating Cutaneous Wound Healing

Advanced Healthcare Materials ◽

10.1002/adhm.202102027 ◽

2021 ◽

pp. 2102027

Author(s):

Hyosuk Kim ◽

Da Eun Kim ◽

Geonhee Han ◽

Nu Ri Lim ◽

Eun Hye Kim ◽

...

Keyword(s):

Wound Healing ◽

Extracellular Vesicles ◽

Tissue Regeneration ◽

Bovine Milk ◽

Cutaneous Wound Healing ◽

Cutaneous Wound ◽

Healing Power ◽

Natural Healing

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MSC-Derived Exosome Promotes M2 Polarization and Enhances Cutaneous Wound Healing

Stem Cells International ◽

10.1155/2019/7132708 ◽

2019 ◽

Vol 2019 ◽

pp. 1-16 ◽

Cited By ~ 20

Author(s):

Xiaoning He ◽

Zhiwei Dong ◽

Yina Cao ◽

Han Wang ◽

Shiyu Liu ◽

...

Keyword(s):

Bone Marrow ◽

Wound Healing ◽

Wound Repair ◽

Macrophage Polarization ◽

Therapeutic Effects ◽

Cutaneous Wound Healing ◽

Bone Marrow Mscs ◽

Wound Site ◽

Cutaneous Wound ◽

M2 Polarization

Mesenchymal stem cell transplantation (MSCT) promotes cutaneous wound healing. Numerous studies have shown that the therapeutic effects of MSCT appear to be mediated by paracrine signaling. However, the cell-cell interaction during MSCT between MSCs and macrophages in the region of cutaneous wound healing is still unknown. In this study, early depletion of macrophages delayed the wound repair with MSC injection, which suggested that MSC-mediated wound healing required macrophages. Moreover, we demonstrated that systemically infused bone marrow MSCs (BMMSCs) and jaw bone marrow MSCs (JMMSCs) could translocate to the wound site, promote macrophages toward M2 polarization, and enhance wound healing.In vitrococulture of MSCs with macrophages enhanced their M2 polarization. Mechanistically, we found that exosomes derived from MSCs induced macrophage polarization and depletion of exosomes of MSCs reduced the M2 phenotype of macrophages. Infusing MSCs without exosomes led to lower number of M2 macrophages at the wound site along with delayed wound repair. We further showed that the miR-223, derived from exosomes of MSCs, regulated macrophage polarization by targeting pknox1. These findings provided the evidence that MSCT elicits M2 polarization of macrophages and may accelerate wound healing by transferring exosome-derived microRNA.

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Advances of Stem Cell Therapeutics in Cutaneous Wound Healing and Regeneration

Mediators of Inflammation ◽

10.1155/2017/5217967 ◽

2017 ◽

Vol 2017 ◽

pp. 1-14 ◽

Cited By ~ 63

Author(s):

Suman Kanji ◽

Hiranmoy Das

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Stem Cell ◽

Wound Repair ◽

Chronic Wounds ◽

Healing Process ◽

Treatment Modalities ◽

Wound Healing Process ◽

Cutaneous Wound Healing ◽

Cutaneous Wound

Cutaneous wound healing is a complex multiple phase process, which overlaps each other, where several growth factors, cytokines, chemokines, and various cells interact in a well-orchestrated manner. However, an imbalance in any of these phases and factors may lead to disruption in harmony of normal wound healing process, resulting in transformation towards chronic nonhealing wounds and abnormal scar formation. Although various therapeutic interventions are available to treat chronic wounds, current wound-care has met with limited success. Progenitor stem cells possess potential therapeutic ability to overcome limitations of the present treatments as it offers accelerated wound repair with tissue regeneration. A substantial number of stem cell therapies for cutaneous wounds are currently under development as a result of encouraging preliminary findings in both preclinical and clinical studies. However, the mechanisms by which these stem cells contribute to the healing process have yet to be elucidated. In this review, we emphasize on the major treatment modalities currently available for the treatment of the wound, role of various interstitial stem cells and exogenous adult stem cells in cutaneous wound healing, and possible mechanisms involved in the healing process.

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Improvement of Cutaneous Wound Healing via Topical Application of Heat-Killed Lactococcus chungangensis CAU 1447 on Diabetic Mice

Nutrients ◽

10.3390/nu13082666 ◽

2021 ◽

Vol 13 (8) ◽

pp. 2666

Author(s):

YoHan Nam ◽

Jong-Hwa Kim ◽

Jihye Baek ◽

Wonyong Kim

Keyword(s):

Wound Healing ◽

Wound Repair ◽

Therapeutic Agents ◽

Skin Wound ◽

Skin Microbiome ◽

Cutaneous Wound Healing ◽

Diabetic Mice ◽

Cutaneous Wound ◽

Beneficial Effects ◽

Systemic Network

Cutaneous wound healing comprises a complex systemic network. Probiotics, naturally extracted substances, medicine, and chemical compounds have been used for wound healing, but the application of postbiotics as therapeutic agents has yet to be explored. Our study shows potential beneficial effects of heat-killed Lactococcus chungangensis CAU 1447 on type 1 diabetic mice. The postbiotic strain significantly decreased the skin wound size. The activity of myeloperoxidase secreted from neutrophils also decreased. The molecular mechanism of wound healing was adjusted by important mediators, growth factors, chemokines, and cytokines. These elements regulated the anti-inflammatory activity and accelerated wound healing. To determine the role of the postbiotic in wound repair, we showed a similar taxonomic pattern as compared to the diabetic mice using skin microbiome analysis. These findings demonstrated that heat-killed Lactococcus chungangensis CAU 1447 had beneficial effects on wound healing and can be utilized as postbiotic therapeutic agents.

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SPARC-thrombospondin-2-double-null Mice Exhibit Enhanced Cutaneous Wound Healing and Increased Fibrovascular Invasion of Subcutaneous Polyvinyl Alcohol Sponges

Journal of Histochemistry & Cytochemistry ◽

10.1369/jhc.4a6425.2005 ◽

2005 ◽

Vol 53 (5) ◽

pp. 571-581 ◽

Cited By ~ 24

Author(s):

Pauli A. Puolakkainen ◽

Amy D. Bradshaw ◽

Rolf A. Brekken ◽

May J. Reed ◽

Themis Kyriakides ◽

...

Keyword(s):

Wound Healing ◽

Wound Repair ◽

Dermal Fibroblasts ◽

Fine Tuning ◽

Cutaneous Wound Healing ◽

Collagen Gels ◽

Matricellular Proteins ◽

Cutaneous Wound ◽

Cutaneous Wounds

Secreted protein acidic and rich in cysteine (SPARC) and thrombospondin-2 (TSP-2) are structurally unrelated matricellular proteins that have important roles in cell- extracellular matrix (ECM) interactions and tissue repair. SPARC-null mice exhibit accelerated wound closure, and TSP-2-null mice show an overall enhancement in wound healing. To assess potential compensation of one protein for the other, we examined cutaneous wound healing and fibrovascular invasion of subcutaneous sponges in SPARC-TSP-2 (ST) double-null and wild-type (WT) mice. Epidermal closure of cutaneous wounds was found to occur significantly faster in ST-double-null mice, compared with WT animals: histological analysis of dermal wound repair revealed significantly more mature phases of healing at 1, 4, 7, 10, and 14 days after wounding, and electron microscopy showed disrupted ECM at 14 days in these mice. ST-double-null dermal fibroblasts displayed accelerated migration, relative to WT fibroblasts, in a wounding assay in vitro, as well as enhanced contraction of native collagen gels. Zymography indicated that fibroblasts from ST-double-null mice also produced higher levels of matrix metalloproteinase (MMP)-2. These data are consistent with the increased fibrovascular invasion of subcutaneous sponge implants seen in the double-null mice. The generally accelerated wound healing of ST-double-null mice reflects that described for the single-null animals. Importantly, the absence of both proteins results in elevated MMP-2 levels. SPARC and TSP-2 therefore perform similar functions in the regulation of cutaneous wound healing, but fine-tuning with respect to ECM production and remodeling could account for the enhanced response seen in ST-double-null mice.

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The Angiogenic Factor Cyr61 Activates a Genetic Program for Wound Healing in Human Skin Fibroblasts

Journal of Biological Chemistry ◽

10.1074/jbc.m107666200 ◽

2001 ◽

Vol 276 (50) ◽

pp. 47329-47337 ◽

Cited By ~ 186

Author(s):

Chih-Chiun Chen ◽

Fan-E Mo ◽

Lester F. Lau

Keyword(s):

Gene Expression ◽

Wound Healing ◽

Wound Repair ◽

Skin Fibroblasts ◽

Matrix Remodeling ◽

Cutaneous Wound Healing ◽

Heparin Binding ◽

Human Skin Fibroblasts ◽

Cutaneous Wound

Cyr61 is a heparin-binding, extracellular matrix-associated protein of the CCN family, which also includes connective tissue growth factor, Nov, WISP-1, WISP-2, and WISP-3. Cyr61 is capable of multiple functions, including induction of angiogenesisin vivo. Purified Cyr61 mediates cell adhesion and induces adhesive signaling, stimulates cell migration, enhances cell proliferation, and promotes cell survival in both fibroblasts and endothelial cells. In this study, we have used cDNA array hybridization to identify genes regulated by Cyr61 in primary human skin fibroblasts. The Cyr61-regulated genes fall into several groups known to participate in processes important for cutaneous wound healing, including: 1) angiogenesis and lymphogenesis (VEGF-A and VEGF-C); 2) inflammation (interleukin-1β); 3) extracellular matrix remodeling (MMP1, MMP3, TIMP1, uPA, and PAI-1); and 4) cell-matrix interactions (Col1α1, Col1α2, and integrins α3and α5). Cyr61-mediated gene expression requires heparin binding activity of Cyr61, cellularde novotranscription, and protein synthesis and is largely dependent on the activation of p42/p44 MAPKs. Cyr61 regulates gene expression not only in serum-free medium but also in fibroblasts cultured on various matrix proteins or in the presence of 10% serum. These effects of Cyr61 can be sustained for at least 5 days, consistent with the time course of wound healingin vivo. Interestingly, Cyr61 can interact with transforming growth factor-β1 to regulate expression of specific genes in an antagonistic, additive, or synergistic manner. Furthermore, we show that theCyr61gene is highly induced in dermal fibroblasts of granulation tissue during cutaneous wound repair. Together, these results show thatCyr61is inducibly expressed in granulation tissues after wounding and that Cyr61 activates a genetic program for wound repair in skin fibroblasts. We propose a model in which Cyr61 integrates its activities on endothelial cells, fibroblasts, and macrophages to regulate the processes of angiogenesis, inflammation, and matrix remodeling in the context of cutaneous wound healing.

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