Branched lipid chains to prepare cationic amphiphiles producing hexagonal aggregates: supramolecular behavior and application to gene delivery

2020 ◽  
Vol 18 (2) ◽  
pp. 337-345
Author(s):  
Amal Bouraoui ◽  
Rosy Ghanem ◽  
Mathieu Berchel ◽  
Laure Deschamps ◽  
Véronique Vié ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cationic Amphiphiles ◽  
Self Organized ◽  
High Gene

Cationic amphiphiles featuring ramified lipid chains self-organized in water as inverted hexagonal aggregates. They demonstrated high gene delivery efficiencies.

scholarly journals 177. A Novel Class of Cationic Amphiphiles for Airway Gene Delivery

2006 ◽  
Vol 13 ◽  
pp. S68-S69
Author(s):  
Keun-Sik Kim ◽  
Qun Li ◽  
Takeshi Suda ◽  
Dexi Liu
Keyword(s):  
Gene Delivery ◽  
Cationic Amphiphiles

scholarly journals FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-β-CyD

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Yiping Hu ◽  
Guping Tang ◽  
Jun Liu ◽  
Wenxiang Cheng ◽  
Ye Yue ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Human Cancer ◽  
Fibroblast Growth Factor Receptors ◽  
Fgf Receptor ◽  
Human Cancer Cells ◽  
High Gene ◽  
Targeting Ability ◽  
Delivery Efficiency

A novel vector with high gene delivery efficiency and special cell-targeting ability was developed using a good strategy that utilized low-molecular-weight polyethylenimine (PEI; molecular weight: 600 KDa [PEI600]) crosslinked toβ-cyclodextrin (β-CyD) via a facile synthetic route. Fibroblast growth factor receptors (FGFRs) are highly expressed in a variety of human cancer cells and are potential targets for cancer therapy. In this paper, CY11 peptides, which have been proven to combine especially with FGFRs on cell membranes were coupled to PEI-β-CyD usingN-succinimidyl-3-(2-pyridyldithio) propionate as a linker. The ratios of PEI600,β-CyD, and peptide were calculated based on proton integral values obtained from the1H-NMR spectra of the resulting products. Electron microscope observations showed that CY11-PEI-β-CyD can efficiently condense plasmid DNA (pDNA) into nanoparticles of about 200 nm, and MTT assays suggested the decreased toxicity of the polymer. Experiments on gene delivery efficiency in vitro showed that CY11-PEI-β-CyD/pDNA polyplexes had significantly greater transgene activities than PEI-β-CyD/pDNA in the COS-7 and HepG2 cells, which positively expressed FGFR, whereas no such effect was observed in the PC-3 cells, which negatively expressed FGFR. Our current research indicated that the synthesized nonviral vector shows improved gene delivery efficiency and targeting specificity in FGFR-positive cells.

scholarly journals Bis‐Thioether‐Containing Lipid Chains in Cationic Amphiphiles: Physicochemical Properties and Applications in Gene Delivery

ChemPhysChem ◽  
2019 ◽  
Vol 20 (17) ◽  
pp. 2187-2194
Author(s):  
Amal Bouraoui ◽  
Rosy Ghanem ◽  
Mathieu Berchel ◽  
Véronique Vié ◽  
Yann Le Guen ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Physicochemical Properties ◽  
Cationic Amphiphiles

Self-assembling Janus dendritic polymer for gene delivery with low cytotoxicity and high gene transfection efficiency

2016 ◽  
Vol 4 (39) ◽  
pp. 6462-6467 ◽  
Author(s):  
Sheng-Gang Ding ◽  
Lei Yu ◽  
Long-Hai Wang ◽  
Lin-Ding Wang ◽  
Zhi-Qiang Yu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Dendritic Polymer ◽  
Self Assembling ◽  
High Gene ◽  
Low Cytotoxicity

Polycations have high DNA condensing ability, low immunogenicity, and great adaptability, which make them promising for gene delivery.

scholarly journals Lipophilic Polyamines as Promising Components of Liposomal Gene Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 920
Author(s):  
Pavel A. Puchkov ◽  
Michael A. Maslov
Keyword(s):  
Gene Therapy ◽  
Gene Delivery ◽  
Nucleic Acids ◽  
Mutual Influence ◽  
Structural Components ◽  
Delivery Vehicle ◽  
Safe Delivery ◽  
Cationic Amphiphiles ◽  
Liposomal Formulations ◽  
Delivery Efficiency

Gene therapy requires an effective and safe delivery vehicle for nucleic acids. In the case of non-viral vehicles, including cationic liposomes, the structure of compounds composing them determines the efficiency a lot. Currently, cationic amphiphiles are the most frequently used compounds in liposomal formulations. In their structure, which is a combination of hydrophobic and cationic domains and includes spacer groups, each component contributes to the resulting delivery efficiency. This review focuses on polycationic and disulfide amphiphiles as prospective cationic amphiphiles for gene therapy and includes a discussion of the mutual influence of structural components.

Aza-crown ether locked on polyethyleneimine: solving the contradiction between transfection efficiency and safety during in vivo gene delivery

2020 ◽  
Vol 56 (41) ◽  
pp. 5552-5555
Author(s):  
Shengran Li ◽  
Lin Lin ◽  
Wenliang Wang ◽  
Xinxin Yan ◽  
Binggang Chen ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Crown Ether ◽  
Tumor Targeting ◽  
Transfection Efficiency ◽  
High Gene ◽  
In Vivo Gene Delivery ◽  
Targeting Ability ◽  
Extended Circulation ◽  
Aza Crown Ether

An aza-crown ether derivative to lock a hyperbranched PEI, which endows the PEI with tumor targeting ability, antiserum ability and extended circulation in the blood, meanwhile retaining the high gene complexation and high transfection efficiency.

scholarly journals Substitution of unsaturated lipid chains by thioether-containing lipid chains in cationic amphiphiles: physicochemical consequences and application for gene delivery

2019 ◽  
Vol 17 (14) ◽  
pp. 3609-3616 ◽  
Author(s):  
Amal Bouraoui ◽  
Mathieu Berchel ◽  
Rosy Ghanem ◽  
Véronique Vié ◽  
Gilles Paboeuf ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Unsaturated Lipid ◽  
Cationic Amphiphiles

New thioether-containing amphiphiles were designed, characterized and applied for gene delivery.

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