Substitution of unsaturated lipid chains by thioether-containing lipid chains in cationic amphiphiles: physicochemical consequences and application for gene delivery

Gene therapy requires an effective and safe delivery vehicle for nucleic acids. In the case of non-viral vehicles, including cationic liposomes, the structure of compounds composing them determines the efficiency a lot. Currently, cationic amphiphiles are the most frequently used compounds in liposomal formulations. In their structure, which is a combination of hydrophobic and cationic domains and includes spacer groups, each component contributes to the resulting delivery efficiency. This review focuses on polycationic and disulfide amphiphiles as prospective cationic amphiphiles for gene therapy and includes a discussion of the mutual influence of structural components.

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Branched lipid chains to prepare cationic amphiphiles producing hexagonal aggregates: supramolecular behavior and application to gene delivery

Organic & Biomolecular Chemistry ◽

10.1039/c9ob02381j ◽

2020 ◽

Vol 18 (2) ◽

pp. 337-345

Author(s):

Amal Bouraoui ◽

Rosy Ghanem ◽

Mathieu Berchel ◽

Laure Deschamps ◽

Véronique Vié ◽

...

Keyword(s):

Gene Delivery ◽

Cationic Amphiphiles ◽

Self Organized ◽

High Gene

Cationic amphiphiles featuring ramified lipid chains self-organized in water as inverted hexagonal aggregates. They demonstrated high gene delivery efficiencies.

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α-Tocopherol-based cationic amphiphiles with a novel pH sensitive hybrid linker for gene delivery

Organic & Biomolecular Chemistry ◽

10.1039/c8ob00276b ◽

2018 ◽

Vol 16 (16) ◽

pp. 2932-2946 ◽

Cited By ~ 4

Author(s):

Venkanna Muripiti ◽

Hari Krishnareddy Rachamalla ◽

Rajkumar Banerjee ◽

Srilakshmi. V. Patri

Keyword(s):

Gene Delivery ◽

Ph Sensitive ◽

Endosomal Escape ◽

Cationic Amphiphiles

Endosomal escape is one of the barriers for the efficient liposomal gene delivery.

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Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161151 ◽

1990 ◽

Vol 48 (3) ◽

pp. 718-719

Author(s):

Dai Dalin ◽

Guo Jianmin

Keyword(s):

Electron Microscope ◽

Polyethylene Glycol ◽

Chemical Reaction ◽

Traditional Method ◽

Osmium Tetroxide ◽

Unsaturated Lipid ◽

Embedding Method ◽

Long Period ◽

New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

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