Paper-based microfluidic devices based on 3D network polymer hydrogel for the determination of glucose in human whole blood

In this study, optical microfluidic paper analytical devices (μPADs) for glucose detection from whole blood samples with a small sample volume (2 μL) have been developed on a single paper.

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Determination of quinalphos in human whole blood samples by high-performance thin-layer chromatography for forensic applications

Journal of Chromatography A ◽

10.1016/j.chroma.2019.02.003 ◽

2019 ◽

Vol 1594 ◽

pp. 181-189 ◽

Cited By ~ 2

Author(s):

Praveen U Sanganalmath ◽

Purigali M Nagaraju ◽

Kuruba Sreeramulu

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

Whole Blood ◽

High Performance ◽

Blood Samples ◽

Human Whole Blood ◽

Whole Blood Samples ◽

Layer Chromatography

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Determination of Hemoglobin in Human Whole Blood Using Near-Infrared Spectroscopy with Stability

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.566.660 ◽

2012 ◽

Vol 566 ◽

pp. 660-663

Author(s):

Zhen Yao Liu ◽

Tao Pan

Keyword(s):

Whole Blood ◽

Near Infrared ◽

Nir Spectroscopy ◽

Short Wave ◽

Blood Samples ◽

Human Whole Blood ◽

Modeling Process ◽

High Prediction ◽

Validation Set

Near-infrared (NIR) spectroscopy combined with the partial least-squares (PLS) regression was successfully applied for the rapid quantitative analysis of hemoglobin (HGB) based on human whole blood samples. Based on the varied divisions for the calibration and prediction sets, an appropriate waveband with stability was selected through a rigorous modeling process. Among 215 samples, 80 were randomly selected as the validation set. The remaining 135 samples were divided into the calibration set (80 samples) and the prediction set (55 samples) for a total of 200 times with certain similarities. The selected waveband was the combination of visible and short-wave NIR region at 400 nm to 1100 nm. The optimal PLS factor was 8, the modeling effect M-SEPAve, M-RP,Ave, M-SEPStd and M-RP,Std were 2.88gL-1, 0.986, 0.34gL-1 and 0.003, respectively, the validation effect V-SEP, V-RP and V-RSEP were 2.92gL-1, 0.986 and 2.26%, respectively. The results indicated that the method has high prediction precision and well stability. It is hopeful to be applied to clinic.

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Determination of 19 Psychoactive Substances in Premortem and Postmortem Whole Blood Samples Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Separations ◽

10.3390/separations8060078 ◽

2021 ◽

Vol 8 (6) ◽

pp. 78

Author(s):

Sevasti Karampela ◽

Jessica Smith ◽

Irene Panderi

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Formic Acid ◽

Whole Blood ◽

High Performance ◽

Tandem Mass ◽

Psychoactive Substances ◽

Blood Samples ◽

Whole Blood Samples

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

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Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

STAR Protocols ◽

10.1016/j.xpro.2021.100311 ◽

2021 ◽

Vol 2 (1) ◽

pp. 100311

Author(s):

Daniella C. Terenzi ◽

Ehab Bakbak ◽

Justin Z. Trac ◽

Mohammad Al-Omran ◽

Adrian Quan ◽

...

Keyword(s):

Whole Blood ◽

Progenitor Cell ◽

Blood Samples ◽

Human Whole Blood ◽

Isolation And Characterization ◽

Whole Blood Samples

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The distribution of BrKα/RbKα peak intensity ratio in human whole blood samples

Biological Trace Element Research ◽

10.1007/bf02911520 ◽

1994 ◽

Vol 42 (3) ◽

pp. 231-241 ◽

Cited By ~ 2

Author(s):

C. Shenberg ◽

S. Spiegel ◽

S. Chaitchik ◽

P. Jordan ◽

M. Kitzis ◽

...

Keyword(s):

Whole Blood ◽

Intensity Ratio ◽

Peak Intensity Ratio ◽

Blood Samples ◽

Human Whole Blood ◽

Peak Intensity ◽

Whole Blood Samples

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Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS

The Scientific World JOURNAL ◽

10.1100/2012/571201 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 22

Author(s):

Mustafa Karapirli ◽

Murat Kizilgun ◽

Ozgur Yesilyurt ◽

Husamettin Gul ◽

Zeki Ilker Kunak ◽

...

Keyword(s):

Mass Spectrometry ◽

Tandem Mass Spectrometry ◽

Simultaneous Determination ◽

Cyclosporine A ◽

Whole Blood ◽

Immunosuppressive Drugs ◽

Tandem Mass ◽

Blood Samples ◽

Whole Blood Samples

Objectives. Cyclosporine A (CyA), tacrolimus (TRL), sirolimus (SIR), and everolimus (RAD) are immunosuppressive drugs frequently used in organ transplantation. Our aim was to confirm a robust sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of CyA, TRL, SIR, and RAD in whole-blood samples.Materials and Methods. We used an integrated online solid-phase extraction-LC-MS/MS system and atmospheric pressure ionization tandem mass spectrometry (API-MS/MS) in the multiple reaction monitoring (MRM) detection mode. CyA, TRL, SIR, and RAD were simultaneously analyzed in whole blood treated with precipitation reagent taken from transplant patients.Results. System performance parameters were suitable for using this method as a high-throughput technique in clinical practice. The high concentration of one analyte in the sample did not affect the concentration of other analytes. Total analytical time was 2.5 min, and retention times of all analytes were shorter than 2 minutes.Conclusion. This LC-MS/MS method can be preferable for therapeutic drug monitoring of these immunosuppressive drugs (CyA, TRL, SRL, and RAD) in whole blood. Sample preparation was too short and simple in this method, and it permits robust, rapid, sensitive, selective, and simultaneous determination of these drugs.

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