Efficient photosensitizers with aggregation-induced emission characteristics for lysosome- and Gram-positive bacteria-targeted photodynamic therapy

2020 ◽  
Vol 56 (17) ◽  
pp. 2630-2633 ◽  
Author(s):  
Jiabao Zhuang ◽  
Hanxiao Yang ◽  
Yue Li ◽  
Bing Wang ◽  
Nan Li ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Cells ◽  
White Light ◽  
Light Irradiation ◽  
Emission Characteristics ◽  
Gram Positive ◽  
Aggregation Induced Emission ◽  
Gram Positive Bacteria ◽  
Targeted Photodynamic Therapy

Efficient photosensitizers with aggregation-induced emission effects were reported to selectively stain lysosome and Gram-positive bacteria, which further triggered the ablation of cancer cells and bacteria under white light irradiation.

A multifunctional luminogen with aggregation-induced emission characteristics for selective imaging and photodynamic killing of both cancer cells and Gram-positive bacteria

2018 ◽  
Vol 6 (23) ◽  
pp. 3894-3903 ◽  
Author(s):  
Miaomiao Kang ◽  
Ryan T. K. Kwok ◽  
Jianguo Wang ◽  
Han Zhang ◽  
Jacky W. Y. Lam ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Emission Characteristics ◽  
Gram Positive ◽  
Aggregation Induced Emission ◽  
Gram Positive Bacteria ◽  
Positively Charged

A positively charged multifunctional AIEgen was developed for selective imaging and photodynamic killing of cancer cells as well as Gram-positive bacteria.

scholarly journals A Novel Water-Soluble Photosensitizer for Photodynamic Inactivation of Gram-Positive Bacteria

2020 ◽  
Author(s):  
Zihuayuan Yang ◽  
Ying Qiao ◽  
Junying Li ◽  
Fu-Gen Wu ◽  
Fengming Lin
Keyword(s):  
Photodynamic Therapy ◽  
Water Solubility ◽  
Uv Light ◽  
Water Soluble ◽  
Light Irradiation ◽  
Microbial Fermentation ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Uv Light Irradiation

AbstractAntimicrobial photodynamic therapy (APDT) is a promising alternative to traditional antibiotics for bacterial infections, which inactivates a broad spectrum of bacteria. However, it has some disadvantages including poor water solubility and easy aggregation of hydrophobic photosensitizers (PS), and poor tissue penetration and cytotoxicity when using UV as the light source, leading to undesired photodynamic therapy efficacy. Herein, we develop a novel water-soluble natural PS (sorbicillinoids) obtained by microbial fermentation using recombinant filamentous fungus Trichoderma reesei (T. reesei). Sorbicillinoids could effectively generate singlet oxygen (1O2) under ultraviolet (UV) light irradiation, and ultimately display photoinactivation activity on Gram-positive bacteria, but not Gram-negative ones. Staphylococcus aureus (S. aureus) treated with sorbicillinoids and UV light displayed high levels of intracellular reactive oxygen species (ROS), notable DNA photocleavage, and compromised cell semi-permeability without overt cell membrane disruption. Moreover, the dark toxicity, phototoxicity or hemolysis activity of sorbicillinoids is negligible, showing its excellent biocompatibility. Therefore, sorbicillinoids, a type of secondary metabolite from fungus, has a promising future as a new PS for APDT using nontoxic dose of UV light irradiation.ImportanceIt is of great value to develop novel PSs for APDT to enhance its efficacy for the reason that many traditional PSs have disadvantages like low water solubility and poor biocompatibility. In this study, we develop a novel water-soluble natural PS - sorbicillinoids obtained by microbial fermentation using T. reesei. Sorbicillinoids could effectively generate singlet oxygen under UV light irradiation, and ultimately display photoinactivation activity on Gram-positive bacteria, but not Gram-negative ones. More importantly, UV light can generally only be used to inactivate bacteria on the surface due to its weak penetration. However, it can penetrate deep into the solution and inactivate bacteria in the presence of sorbicillinoids. Therefore, sorbicillinoids, a type of secondary metabolite from fungus, has a promising future as a new PS for APDT using nontoxic dose of UV light irradiation.

Aluminum phthalocyanine and gold nanorod conjugates: the combination of photodynamic therapy and photothermal therapy to kill cancer cells

2012 ◽  
Vol 16 (07n08) ◽  
pp. 802-808 ◽  
Author(s):  
J. Wang ◽  
H. Yan Tang ◽  
W. Li Yang ◽  
J. Yao Chen
Keyword(s):  
Photodynamic Therapy ◽  
Cell Viability ◽  
Cancer Cells ◽  
White Light ◽  
Photothermal Therapy ◽  
Red Light ◽  
Carcinoma Cells ◽  
Light Irradiation ◽  
Surface Plasmon Coupling ◽  
Electrostatic Binding

The sulfonated aluminum phthalocyanines (AlPcSs), popularly used photosensitizers, were linked on the surfaces of gold nanorods (AuNRs) by the electrostatic binding to form AlPcS–AuNRs conjugates, in order to improve the photo-therapy efficiency of cancer cells by combining the photodynamic therapy (PDT) of AlPcSs and the photothermal therapy (PTT) of AuNRs . The AlPcS's fluorescence is two-fold enhanced when they adhered on the surfaces of AuNRs probably due to the surface Plasmon coupling, which would facilitate the AlPcS detection. The fluorescence images show that AuNRs can carry loaded AlPcSs to penetrate into human nasopharyngeal carcinoma cells with a fast speed, achieving the effective intracellular delivery of AlPcSs . The PTT effect of cellular AuNRs alone under the white light irradiation of 50 minutes decreased the cell viability to 77%, and the PDT effect of cellular AlPcS–AuNRs with filtered red light (670–710 nm) irradiation of 50 min lowered the cell viability to 79%. However, with the same white light irradiation of 50 min, the AlPcS–AuNRs destroyed most cells leaving the cell viability to 28%, reflecting a typical synergistic effect on cell killing. These results suggest that the combination of PTT and PDT with AlPcS–AuNRs is a promising strategy for improving the phototherapy of cancers.

AIE based GSH activatable photosensitizer for imaging-guided photodynamic therapy

2020 ◽  
Vol 56 (71) ◽  
pp. 10317-10320 ◽  
Author(s):  
You-Hui Zhang ◽  
Xue Li ◽  
Li Huang ◽  
Hyeong Seok Kim ◽  
Jusung An ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Emission Characteristics ◽  
Aggregation Induced Emission ◽  
Photodynamic Therapy Of Cancer

A GSH activatable photosensitizer with aggregation-induced emission characteristics for imaging-guided photodynamic therapy of cancer cells.

scholarly journals Novel Water-Soluble Photosensitizer for Photodynamic Inactivation of Gram-Positive Bacteria Using Nontoxic Dose of UV Light

2020 ◽  
Author(s):  
Zihuayuan Yang ◽  
Ying Qiao ◽  
Junying Li ◽  
Fu-Gen Wu ◽  
Fengming Lin
Keyword(s):  
Photodynamic Therapy ◽  
Bacterial Infections ◽  
Water Solubility ◽  
Uv Light ◽  
Water Soluble ◽  
Light Irradiation ◽  
Gram Positive ◽  
Promising Alternative ◽  
Gram Positive Bacteria ◽  
Uv Light Irradiation

Abstract Background Antimicrobial photodynamic therapy (APDT) is a promising alternative to traditional antibiotics for bacterial infections, which inactivates a broad spectrum of bacteria. However, it has some disadvantages including poor water solubility and easy aggregation of hydrophobic photosensitizers (PS), and poor tissue penetration and cytotoxicity when using UV as the light source, leading to undesired photodynamic therapy efficacy.Results In this study, we develop a novel water-soluble natural PS (sorbicillinoids) obtained by microbial fermentation using recombinant filamentous fungus Trichoderma reesei (T. reesei). Sorbicillinoids could effectively generate singlet oxygen (1O2) under ultraviolet (UV) light irradiation, and ultimately display photoinactivation activity on Gram-positive bacteria, but not Gram-negative ones. Staphylococcus aureus (S. aureus) treated with sorbicillinoids and UV light displayed high levels of intracellular reactive oxygen species (ROS), notable DNA photocleavage, and compromised cell semi-permeability without overt cell membrane disruption. Moreover, the dark toxicity, phototoxicity or hemolysis activity of sorbicillinoids is negligible, showing its excellent biocompatibility.Conclusion Sorbicillinoids obtained from T. reesei display photoinactivation activity on Gram-positive bacteria using nontoxic dose of UV light irradiation and have an excellent biocompatibility Therefore, sorbicillinoids, a type of secondary metabolite from fungus, has a promising future as a new PS for APDT.

scholarly journals Aggregation-Induced Emission (AIE) Polymeric Micelles for Imaging-Guided Photodynamic Cancer Therapy

Nanomaterials ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 921 ◽  
Author(s):  
Yang Zhang ◽  
Cai-Xia Wang ◽  
Shi-Wen Huang
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Plasma Membranes ◽  
Polymeric Micelles ◽  
Light Irradiation ◽  
Emission Characteristic ◽  
Noninvasive Treatment ◽  
Aggregation Induced Emission ◽  
Photodynamic Cancer Therapy

Photodynamic therapy (PDT) is a noninvasive treatment for selectively killing malignant tumor cells. The photosensitizer is a necessary component of photodynamic nanomedicine. Many efforts have been made to develop new photosensitizers for efficient cancer photodynamic therapy. In this work, we report a novel nano photosensitizer, polymeric micelles (AIE-M) with aggregation induced emission characteristic, for photodynamic cancer therapy. AIE-M with sub-20 nm particle size is prepared by the self-assembly of salicylaldazine-incorporated amphiphilic polymer (AIE-1), which can produce reactive oxygen species (ROS) with light irradiation in solution. After uptake by cancer cells, AIE-M can specially sojourn in plasma membranes of cancer cells at the early stage and predominantly accumulate in the mitochondria of cancer cell at the late stage. The phototoxicity of AIE-M, resulting from the generation of intracellular ROS with light irradiation, can efficiently cause cancer cells death by apoptosis and necrosis. The advantages of AIE-M as a nano photosensitizer include the small size, highly colloidal stability in the process of preparation and storage, and high cell penetration. The ultra-low Critical Micelle Concentration (CMC) of AIE-1, negligible dark toxicity and super phototoxicity of AIE-M suggest its promising potential for image-guided PDT.

Cyclometalated iridium(iii) complex nanoparticles for mitochondria-targeted photodynamic therapy

Nanoscale ◽  
2020 ◽  
Vol 12 (26) ◽  
pp. 14061-14067 ◽  
Author(s):  
Huan Lu ◽  
Xinpeng Jiang ◽  
Yanyan Chen ◽  
Ke Peng ◽  
Yiming Huang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
White Light ◽  
Breast Cancer Cells ◽  
Water Solubility ◽  
Targeting Ability ◽  
Targeted Photodynamic Therapy ◽  
Mitochondria Targeting ◽  
Mcf 7

Ir(tiq)2ppy nanoparticles are prepared to achieve water solubility and mitochondria-targeting ability with high PDT efficiency to MCF-7 breast cancer cells under white light irradiation.

A light-up probe with aggregation-induced emission characteristics (AIE) for selective imaging, naked-eye detection and photodynamic killing of Gram-positive bacteria

2015 ◽  
Vol 51 (62) ◽  
pp. 12490-12493 ◽  
Author(s):  
Guangxue Feng ◽  
Youyong Yuan ◽  
Hu Fang ◽  
Ruoyu Zhang ◽  
Bengang Xing ◽  
...  
Keyword(s):  
Selective Recognition ◽  
Emission Characteristics ◽  
Eye Detection ◽  
Gram Positive ◽  
Aggregation Induced Emission ◽  
Gram Positive Bacteria ◽  
Naked Eye ◽  
Resistant Strains ◽  
Vancomycin Resistant ◽  
Naked Eye Detection

We report a multifunctional light-up probe based on AIEgens for selective recognition, naked-eye identification, and photodynamic killing of Gram-positive bacteria including vancomycin-resistant strains.

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