Alkaloids and lignans with acetylcholinesterase inhibitory activity from the flower buds of Magnolia biondii Pamp

GC-MS (gas chromatography-mass spectrometry) analyses of alkaloids in the aerial parts and bulbs of Galanthus rizehensis Stern (Amaryllidaceae), collected during two different vegetation periods, was performed. Twenty three alkaloids were identified in four different alkaloid extracts. Acetylcholinesterase (AChE) inhibitory activities of the alkaloid extracts were tested. Both the highest alkaloid diversity and the most potent inhibitory activity (IC50 12.94 μg/ml) were obtained in extracts from the bulbs of G. rizehensis collected during the fruiting period.

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Synthesis, in vitro Acetylcholinesterase Inhibitory Activity Evaluation and Docking Investigation of Some Aromatic Chalcones

MedPharmRes ◽

10.32895/ump.mpr.1.1.15/suffix ◽

2017 ◽

Vol 1 (1) ◽

pp. 15-25

Author(s):

Dao Tran ◽

Son Tran ◽

Vi Nguyen ◽

Tri Le ◽

Minh Thai ◽

...

Keyword(s):

Inhibitory Activity ◽

Condensation Reaction ◽

Acetylcholinesterase Inhibitors ◽

Docking Studies ◽

Acetylcholinesterase Inhibitory Activity ◽

Ic50 Value ◽

Ic50 Values ◽

Inhibitory Activities ◽

Ellman’S Method

In this study, a total of twenty chalcones were synthesized via Claisen-Schmidt condensation reaction and evaluated for their in vitro acetylcholinesterase inhibitory activities using Ellman’s method. Molecular docking studies on acetylcholinesterase were performed to elucidate the interactions between these chalcone derivatives and acetylcholinesterase active site at the molecular level. From the series, six compounds (S1-5 and S17) exhibited strong acetylcholinesterase inhibitory activities with IC50 values below 100 µM compared to the parent unsubstituted chalcone. Compound S17 (4’-amino-2-chlorochalcone) showed the strongest acetylcholinesterase inhibitory activity in the investigated group with IC50 value of 36.10 µM. Molecular modeling studies were consistent with the results of in vitro acetylcholinesterase inhibitory activities, and chalcone S17 could be considered as a potential lead compound for the development of new acetylcholinesterase inhibitors.

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Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria

Zeitschrift für Naturforschung C ◽

10.1515/znc-2014-4179 ◽

2016 ◽

Vol 71 (1-2) ◽

pp. 9-14 ◽

Cited By ~ 5

Author(s):

Radka Z. Vrancheva ◽

Ivan G. Ivanov ◽

Ina Y. Aneva ◽

Ivayla N. Dincheva ◽

Ilian K. Badjakov ◽

...

Keyword(s):

Inhibitory Activity ◽

Hplc Analysis ◽

Acetylcholinesterase Activity ◽

Isoquinoline Alkaloids ◽

Acetylcholinesterase Inhibitory Activity ◽

Ms Analysis ◽

Inhibitory Activities ◽

Alkaloid Extracts ◽

Alkaloid Profiles

Abstract GC-MS analysis of alkaloid profiles of five Fumaria species, naturally grown in Bulgaria (F. officinalis, F. thuretii, F. kralikii, F. rostellata and F. schrammii) and analysis of acetylcholinesterase inhibitory activity of alkaloid extracts were performed. Fourteen isoquinoline alkaloids were identified, with the principle ones being protopine, cryptopine, sinactine, parfumine, fumariline, fumarophycine, and fumaritine. Protopine contents, defined by HPLC analysis varied between 210.6 ± 8.8 μg/g DW (F. schrammii) and 334.5 ± 7.1 μg/g DW. (F. rostellata). While all of the investigated alkaloid extracts significantly inhibited acetylcholinesterase activity, the F. kralikii demonstrated the highest level of inhibition (IC50 0.13 ± 0.01 mg extract/mL).

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STUDY ON BOTANICAL CHARACTERISTICS AND ACETYLCHOLINESTERASE INHIBITORY ACTIVITY OF TWO SPECIES LEEA SP. - LEEACEAE

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2019.4.3 ◽

2019 ◽

pp. 22-29

Author(s):

Thi Bich Hien Le ◽

Thi Minh Quy Le

Keyword(s):

Inhibitory Activity ◽

Antibacterial Effects ◽

Acetylcholinesterase Inhibitory Activity ◽

Osteoarthritis Pain ◽

Microbiological Characteristics ◽

Ic50 Values ◽

Powder Characteristics ◽

Botanical Characteristics ◽

Inhibitory Activities ◽

Dyeing Method

Background: The species belonging to the genus Leea, Leeaceae are reported to have anti-inflammatory, antioxidant, analgesic, antibacterial effects, and have been used to treat osteoarthritis pain, rheumatism, digestive and cancer diseases. The project aims to study botanical characteristics and Acetylcholinesterase inhibitory activity of two species of genus Leea. Materials and method: Two species Leea rubra Blume ex Spreng. and Leea curtisii King., Leeaceae were identified the powder characteristics, microsurgical characteristics by double dyeing method, determined Acetylcholinesterase inhibitory activity by the method of Ellman. Results: Identified characteristics of microsurgery and powder of 2 studied species; total extracts and fractional extracts of 2 studied species shown the average Acetylcholinesterase inhibitory activities with the IC50 values in the range of 42.44 to 97.96 µg/ml. Conclusion: The project contributed to standardize microbiological characteristics and determined Acetylcholinesterase inhibitory activity of two species belonging to the genus Leea. Key words: Acetylcholinesterase, Leea rubra, Leea curtisii, microsurgery, powder characteristics

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