Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria

2016 ◽  
Vol 71 (1-2) ◽  
pp. 9-14 ◽  
Author(s):  
Radka Z. Vrancheva ◽  
Ivan G. Ivanov ◽  
Ina Y. Aneva ◽  
Ivayla N. Dincheva ◽  
Ilian K. Badjakov ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Hplc Analysis ◽  
Acetylcholinesterase Activity ◽  
Isoquinoline Alkaloids ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Ms Analysis ◽  
Inhibitory Activities ◽  
Alkaloid Extracts ◽  
Alkaloid Profiles

Abstract GC-MS analysis of alkaloid profiles of five Fumaria species, naturally grown in Bulgaria (F. officinalis, F. thuretii, F. kralikii, F. rostellata and F. schrammii) and analysis of acetylcholinesterase inhibitory activity of alkaloid extracts were performed. Fourteen isoquinoline alkaloids were identified, with the principle ones being protopine, cryptopine, sinactine, parfumine, fumariline, fumarophycine, and fumaritine. Protopine contents, defined by HPLC analysis varied between 210.6 ± 8.8 μg/g DW (F. schrammii) and 334.5 ± 7.1 μg/g DW. (F. rostellata). While all of the investigated alkaloid extracts significantly inhibited acetylcholinesterase activity, the F. kralikii demonstrated the highest level of inhibition (IC50 0.13 ± 0.01 mg extract/mL).

GC-MS Investigation and Acetylcholinesterase Inhibitory Activity of Galanthus rizehensis

2013 ◽  
Vol 68 (3-4) ◽  
pp. 118-124 ◽  
Author(s):  
Buket Bozkurt Sarikaya ◽  
Nehir Unver Somer ◽  
Gulen Irem Kaya ◽  
Mustafa Ali Onur ◽  
Jaume Bastida ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Inhibitory Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Aerial Parts ◽  
Chromatography Mass Spectrometry ◽  
Inhibitory Activities ◽  
Alkaloid Extracts ◽  
Potent Inhibitory Activity

GC-MS (gas chromatography-mass spectrometry) analyses of alkaloids in the aerial parts and bulbs of Galanthus rizehensis Stern (Amaryllidaceae), collected during two different vegetation periods, was performed. Twenty three alkaloids were identified in four different alkaloid extracts. Acetylcholinesterase (AChE) inhibitory activities of the alkaloid extracts were tested. Both the highest alkaloid diversity and the most potent inhibitory activity (IC50 12.94 μg/ml) were obtained in extracts from the bulbs of G. rizehensis collected during the fruiting period.

scholarly journals Alkaloids from Some Amaryllidaceae Species and Their Cholinesterase Activity

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Lucie Cahlíková ◽  
Nina Benešová ◽  
Kateřina Macáková ◽  
Radim Kučerac ◽  
Václav Hrstka ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Mass Spectra ◽  
Cholinesterase Activity ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Ms Analysis ◽  
Phytochemical Investigation ◽  
Narcissus Poeticus ◽  
Alkaloid Extracts ◽  
Potent Inhibitory Activity

Alkaloid extracts of four Amaryllidaceae species were studied with respect to their acetylcholinesterase and butyrylcholinesterase inhibitory activity and alkaloid patterns. Twenty-one alkaloids were determined by GC/MS, and seventeen of them identified from their mass spectra and retention times. The GC/MS analysis of the alkaloid extract of Nerine filamentosa is the first phytochemical investigation of this species. Promising erythrocytic acetylcholinesterase inhibitory activity was demonstrated by the alkaloid extracts of Narcissus poeticus var recurvus, Nerine filifolia and N. filamentosa (IC50,HuAChE = 6.0 ± 0.1 μg/mL; IC50,HuAChE = 18.5 ± 0.8 μg/mL, IC50,HuAChE = 21.6 ± 1.1 μg/mL). The most potent inhibitory activity against serum butyrylcholinesterase was shown by extracts of Sternbergia lutea and Nerine filamentosa (IC50,HuBuChE = 3.7 ± 0.1 μg/mL; IC50,HuBuChE = 13.0 ± 0.7 μg/mL).

scholarly journals GC-MS Investigation of Amaryllidaceae Alkaloids in Galanthus xvalentinei nothosubsp. subplicatus

2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800
Author(s):  
Buket Bozkurt Sarikaya ◽  
Strahil Berkov ◽  
Jaume Bastida ◽  
Gulen Irem Kaya ◽  
Mustafa Ali Onur ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Anticholinesterase Activity ◽  
Amaryllidaceae Alkaloids ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Ellman’S Method ◽  
First Time

A GC-MS analysis of alkaloids in the aerial parts and bulbs of Galanthus x valentinei nothosubsp. subplicatus was performed for the first time. Totally, twenty-six alkaloids were identified, of which tazettine and galanthindole were the major ones. Acetylcholinesterase inhibitory activity of the alkaloidal extracts was determined using modified in vitro Ellman's method. Significant anticholinesterase activity was observed in the tested samples (bulbs: IC50 = 21.3 μg/mL, aerial parts: IC50 = 16.3 μg/mL).

Alkaloids and lignans with acetylcholinesterase inhibitory activity from the flower buds of Magnolia biondii Pamp

2020 ◽  
Vol 44 (25) ◽  
pp. 10309-10316
Author(s):  
Yangang Cao ◽  
Hongwei Li ◽  
Yanli Zhang ◽  
Jianchao Wang ◽  
Yingjie Ren ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Flower Buds ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Inhibitory Activities

Sixteen compounds, including two new alkaloids and three new lignans, were isolated and their AChE inhibitory activities were evaluated.

scholarly journals Chemical composition and acetylcholinesterase inhibitory activity of essential oil from rhizomes of Distichochlamys benenica

2021 ◽  
Vol 129 (1D) ◽  
pp. 43-49
Author(s):  
Hanh Thi Nhu Hoang ◽  
Thanh Thu Thi Dinh ◽  
Ty Viet Pham ◽  
Hien Bich Thi Le ◽  
Duc Viet Ho
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Inhibitory Activity ◽  
Acetylcholinesterase Activity ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Ic50 Value

Twenty-seven constituents were identified by using GC/MS, representing 99.57% of the rhizome oil of Distichochlamys benenica. The major constituents of the essential oil are 1,8-cineole (54.39%), β-pinene (7.50%), (E)-citral (7.26%), and (Z)-citral (6.79%). The rhizome essential oil has anti-acetylcholinesterase activity with an IC50 value of 136.63 ± 2.70 mg/mL.

Synthesis, in vitro Acetylcholinesterase Inhibitory Activity Evaluation and Docking Investigation of Some Aromatic Chalcones

MedPharmRes ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 15-25
Author(s):  
Dao Tran ◽  
Son Tran ◽  
Vi Nguyen ◽  
Tri Le ◽  
Minh Thai ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Condensation Reaction ◽  
Acetylcholinesterase Inhibitors ◽  
Docking Studies ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Inhibitory Activities ◽  
Ellman’S Method

In this study, a total of twenty chalcones were synthesized via Claisen-Schmidt condensation reaction and evaluated for their in vitro acetylcholinesterase inhibitory activities using Ellman’s method. Molecular docking studies on acetylcholinesterase were performed to elucidate the interactions between these chalcone derivatives and acetylcholinesterase active site at the molecular level. From the series, six compounds (S1-5 and S17) exhibited strong acetylcholinesterase inhibitory activities with IC50 values below 100 µM compared to the parent unsubstituted chalcone. Compound S17 (4’-amino-2-chlorochalcone) showed the strongest acetylcholinesterase inhibitory activity in the investigated group with IC50 value of 36.10 µM. Molecular modeling studies were consistent with the results of in vitro acetylcholinesterase inhibitory activities, and chalcone S17 could be considered as a potential lead compound for the development of new acetylcholinesterase inhibitors.

scholarly journals CHEMICAL CONSTITUENTS AND ACETYLCHOLINESTERASE ACTIVITY FROM Polyalthia sumatrana (Miq.) Kurz

2022 ◽  
Vol 52 (1) ◽  
pp. 73-76
Author(s):  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Natasa Mohd Shakri ◽  
Mohd Azlan Nafiah ◽  
Shamsul Khamis
Keyword(s):  
Cinnamic Acid ◽  
Vanillic Acid ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Acetylcholinesterase Activity ◽  
Isolation And Identification ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Reported Data

This study was carried out to investigate the phytochemicals from Polyalthia sumatrana and their acetylcholinesterase inhibitory activity. Fractionation and purification of the leaves of P. sumatrana led to the isolation and identification of five alkaloids; boldine (1), norboldine (2), liriodenine (3), predicentrine (4), laurotetanine (5) together with β-sitosterol (6), β-sitostenone (7), vanillin (8), vanillic acid (9) and cinnamic acid (10). The structures of these compounds were obtained by analysis of their spectroscopic data, as well as the comparison with that of reported data. Acetylcholinesterase inhibitory activity revealed that all isolated alkaloids were found to inhibit AChE with percentage inhibition values ranged from 45.0 to 80.6%.

STUDY ON BOTANICAL CHARACTERISTICS AND ACETYLCHOLINESTERASE INHIBITORY ACTIVITY OF TWO SPECIES LEEA SP. - LEEACEAE

2019 ◽  
pp. 22-29
Author(s):  
Thi Bich Hien Le ◽  
Thi Minh Quy Le
Keyword(s):  
Inhibitory Activity ◽  
Antibacterial Effects ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Osteoarthritis Pain ◽  
Microbiological Characteristics ◽  
Ic50 Values ◽  
Powder Characteristics ◽  
Botanical Characteristics ◽  
Inhibitory Activities ◽  
Dyeing Method

Background: The species belonging to the genus Leea, Leeaceae are reported to have anti-inflammatory, antioxidant, analgesic, antibacterial effects, and have been used to treat osteoarthritis pain, rheumatism, digestive and cancer diseases. The project aims to study botanical characteristics and Acetylcholinesterase inhibitory activity of two species of genus Leea. Materials and method: Two species Leea rubra Blume ex Spreng. and Leea curtisii King., Leeaceae were identified the powder characteristics, microsurgical characteristics by double dyeing method, determined Acetylcholinesterase inhibitory activity by the method of Ellman. Results: Identified characteristics of microsurgery and powder of 2 studied species; total extracts and fractional extracts of 2 studied species shown the average Acetylcholinesterase inhibitory activities with the IC50 values in the range of 42.44 to 97.96 µg/ml. Conclusion: The project contributed to standardize microbiological characteristics and determined Acetylcholinesterase inhibitory activity of two species belonging to the genus Leea. Key words: Acetylcholinesterase, Leea rubra, Leea curtisii, microsurgery, powder characteristics

Bromotyrosine alkaloids with acetylcholinesterase inhibitory activity from the Thai sponge Acanthodendrilla sp.

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
N Sirimangkalakitti ◽  
M Yokoya ◽  
K Changwichit ◽  
T Saesong ◽  
OJ Olatunji ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Acetylcholinesterase Inhibitory Activity
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