Ruthenium(II) p-cymene complex of pyridine-2-carboxaldehyde and 2-amino benzothiazole based ligand: A cytoselective and in vitro live cell imaging agent†

2021 ◽  
Author(s):  
Anuja Plavuvalapill Kumar ◽  
Priyankar Paira
Keyword(s):  
Ambient Temperature ◽  
Cancer Cells ◽  
General Formula ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Imaging Agent ◽  
Arene Complexes ◽  
Mcf 7

Five Ru(II)-arene complexes ensuring the general formula [(η6-p-cymene)RuCl(k1L1-L5)(pyridine-2-carboxaldehyde)]Cl were synthesized under ambient temperature which exhibited 8-11 fold of cytoselectivity in two cancer cells (HeLa and MCF-7) with respect to normal...

A quinoline-based probe for effective and selective sensing of aspartic acid in aqueous medium: in vitro and in vivo live cell imaging

2019 ◽  
Vol 6 (11) ◽  
pp. 3237-3244 ◽  
Author(s):  
C. Elamathi ◽  
R. J. Butcher ◽  
A. Mohankumar ◽  
P. Sundararaj ◽  
A. Madankumar ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Aspartic Acid ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
C Elegans ◽  
Highly Sensitive ◽  
Mcf 7

A highly sensitive and selective “on–off–on” chemosensor for aspartic acid in aqueous solution was established. In vitro live cell imaging against MCF 7 cells and in vivo imaging using C. elegans were successfully demonstrated.

scholarly journals Real Microgravity Influences the Cytoskeleton and Focal Adhesions in Human Breast Cancer Cells

2019 ◽  
Vol 20 (13) ◽  
pp. 3156 ◽  
Author(s):  
Mohamed Zakaria Nassef ◽  
Sascha Kopp ◽  
Markus Wehland ◽  
Daniela Melnik ◽  
Jayashree Sahana ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Live Cell Imaging ◽  
Breast Cancer Cells ◽  
Cell Imaging ◽  
Live Cell ◽  
Common Mechanism ◽  
Real Microgravity ◽  
Mcf 7 ◽  
E Cadherin

With the increasing number of spaceflights, it is crucial to understand the changes occurring in human cells exposed to real microgravity (r-µg) conditions. We tested the effect of r-µg on MCF-7 breast cancer cells with the objective to investigate cytoskeletal alterations and early changes in the gene expression of factors belonging to the cytoskeleton, extracellular matrix, focal adhesion, and cytokines. In the Technische Experimente unter Schwerelosigkeit (TEXUS) 54 rocket mission, we had the opportunity to conduct our experiment during 6 min of r-µg and focused on cytoskeletal alterations of MCF-7 breast cancer cells expressing the Lifeact-GFP marker protein for the visualization of F-actin as well as the mCherry-tubulin fusion protein using the Fluorescence Microscopy Analysis System (FLUMIAS) for fast live-cell imaging under r-µg. Moreover, in a second mission we investigated changes in RNA transcription and morphology in breast cancer cells exposed to parabolic flight (PF) maneuvers (31st Deutsches Zentrum für Luft- und Raumfahrt (DLR) PF campaign). The MCF-7 cells showed a rearrangement of the F-actin and tubulin with holes, accumulations in the tubulin network, and the appearance of filopodia- and lamellipodia-like structures in the F-actin cytoskeleton shortly after the beginning of the r-µg period. PF maneuvers induced an early up-regulation of KRT8, RDX, TIMP1, CXCL8 mRNAs, and a down-regulation of VCL after the first parabola. E-cadherin protein was significantly reduced and is involved in cell adhesion processes, and plays a significant role in tumorigenesis. Changes in the E-cadherin protein synthesis can lead to tumor progression. Pathway analyses indicate that VCL protein has an activating effect on CDH1. In conclusion, live-cell imaging visualized similar changes as those occurring in thyroid cancer cells in r-µg. This result indicates the presence of a common mechanism of gravity perception and sensation.

A new visible-light-excitable ICT-CHEF-mediated fluorescence ‘turn-on’ probe for the selective detection of Cd2+ in a mixed aqueous system with live-cell imaging

2015 ◽  
Vol 44 (12) ◽  
pp. 5763-5770 ◽  
Author(s):  
Shyamaprosad Goswami ◽  
Krishnendu Aich ◽  
Sangita Das ◽  
Chitrangada Das Mukhopadhyay ◽  
Deblina Sarkar ◽  
...  
Keyword(s):  
Visible Light ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Aqueous System ◽  
Live Cell ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On

A new quinoline based sensor was developed and applied for the selective detection of Cd2+ both in vitro and in vivo.

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

scholarly journals The Use of Live Cell Imaging and Automated Image Analysis to Assist With Determining Optimal Parameters for Angiogenic Assay in vitro

2019 ◽  
Vol 7 ◽  
Author(s):  
Brooke M. Huuskes ◽  
Ryan J. DeBuque ◽  
Peter G. Kerr ◽  
Chrishan S. Samuel ◽  
Sharon D. Ricardo
Keyword(s):  
Image Analysis ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Automated Image Analysis ◽  
Optimal Parameters

Naked-eye detection of Pd2+ ion using a highly selective fluorescent heterocyclic probe by “turn-off” response and in-vitro live cell imaging

2020 ◽  
Vol 394 ◽  
pp. 112441
Author(s):  
Satyajit Mahata ◽  
Araghni Bhattacharya ◽  
Jadi Praveen Kumar ◽  
Biman B. Mandal ◽  
Vadivelu Manivannan
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Eye Detection ◽  
Naked Eye ◽  
Naked Eye Detection

scholarly journals Live-cell imaging of nuclear–chromosomal dynamics in bovine in vitro fertilised embryos

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Tatsuma Yao ◽  
Rie Suzuki ◽  
Natsuki Furuta ◽  
Yuka Suzuki ◽  
Kyoko Kabe ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Live Cell Imaging of In Vitro Human Trophoblast Syncytialization1

2014 ◽  
Vol 90 (6) ◽  
Author(s):  
Rui Wang ◽  
Yan-Li Dang ◽  
Ru Zheng ◽  
Yue Li ◽  
Weiwei Li ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Human Trophoblast

scholarly journals The GTPase-Activating Protein FgGyp1 Is Important for Vegetative Growth, Conidiation, and Virulence and Negatively Regulates DON Biosynthesis in Fusarium graminearium

2021 ◽  
Vol 12 ◽  
Author(s):  
Qiaojia Zheng ◽  
Zhi Yu ◽  
Yanping Yuan ◽  
Danli Sun ◽  
Yakubu Saddeeq Abubakar ◽  
...  
Keyword(s):  
Vegetative Growth ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Negative Regulator ◽  
Rab Gtpase ◽  
Wild Type ◽  
Gtpase Activating Protein ◽  
Replacement Method

Ypt1 is a small Rab GTPase in yeast, Gyp1 functions at the Golgi as a negative regulator of Ypt1. Gyp1 homologs are conserved in filamentous fungi. However, the roles of Gyp1 in phytopathogenic fungi are still unclear. Herein, we investigated the functions of FgGyp1 in the wheat pathogen Fusarium graminearum by live-cell imaging, genetic, and pathological analyses. Targeted gene replacement method was used to delete FgGYP1 in F. graminearum. Phenotypic analyses showed that FgGyp1 is critically important not only for the vegetative growth of F. graminearum but also its conidiation. The mutant’s vegetative growth was significantly reduced by 70% compared to the wild type PH-1. The virulence of FgGYP1 deletion mutant was significantly decreased when compared with the wild type PH-1. We further found that FgGyp1 negatively regulates DON production of the fungus. Live-cell imaging clearly demonstrated that FgGyp1 mainly localizes to the Golgi apparatus. Moreover, the TBC domain, C-terminal, and N-terminal regions of FgGyp1 are found to be indispensable for its biological functions and normal localization. The Arg357 residue of FgGyp1 is essential for its functions but dispensable for the normal localization of the protein, while the Arg284 residue is not required for both the functions and normal localization of the protein. Furthermore, we showed that FgGyp1 essentially hydrolyzes the GTP-bound FgRab1 (activated form) to its corresponding GDP-bound (inactive) form in vitro, suggesting that FgGyp1 is a GTPase-activating protein (GAP) for FgRab1. Finally, FgGyp1 was found to be important for FgSnc1-mediated fusion of secretory vesicles from the Golgi with the plasma membrane in F. graminearum. Put together, these data demonstrate that FgGyp1 functions as a GAP for FgRab1 and is important for vegetative growth, conidiation and virulence, and negatively regulates DON biosynthesis in F. graminearum.

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