Toxicity and translocation of Ag, CuO, ZnO and TiO2 nanoparticles upon exposure to fish intestinal epithelial cells.

2021 ◽  
Author(s):  
Mark Geppert ◽  
Laura Sigg ◽  
Kristin Schirmer
Keyword(s):  
Epithelial Cells ◽  
Human Health ◽  
Tio2 Nanoparticles ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Zno And Tio2 Nanoparticles

Understanding the ability of fish intestinal cells to act as barrier for nanoparticle (NP) uptake and effects is of significance from an environmental perspective but as well for human health,...

Proteomic response of inflammatory stimulated intestinal epithelial cells to in vitro digested plums and cabbages rich in carotenoids and polyphenols

2016 ◽  
Vol 7 (10) ◽  
pp. 4388-4399 ◽  
Author(s):  
Anouk Kaulmann ◽  
Sébastien Planchon ◽  
Jenny Renaut ◽  
Yves-Jacques Schneider ◽  
Lucien Hoffmann ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Bowel Diseases ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Proteomic Response

Proteomic response of intestinal cells as a model of inflammatory bowel diseases to digested plum and cabbage rich in polyphenols and carotenoids.

scholarly journals Caenorhabditis elegans chaperonin CCT/TRiC is required for actin and tubulin biogenesis and microvillus formation in intestinal epithelial cells

2014 ◽  
Vol 25 (20) ◽  
pp. 3095-3104 ◽  
Author(s):  
Keiko Saegusa ◽  
Miyuki Sato ◽  
Katsuya Sato ◽  
Junko Nakajima-Shimada ◽  
Akihiro Harada ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Epithelial Cells ◽  
Apical Membrane ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cells ◽  
Intermediate Filament Protein ◽  
Apical Surface ◽  
Intestinal Epithelial ◽  
Microtubule Network

Intestinal epithelial cells have unique apical membrane structures, known as microvilli, that contain bundles of actin microfilaments. In this study, we report that Caenorhabditis elegans cytosolic chaperonin containing TCP-1 (CCT) is essential for proper formation of microvilli in intestinal cells. In intestinal cells of cct-5(RNAi) animals, a substantial amount of actin is lost from the apical area, forming large aggregates in the cytoplasm, and the apical membrane is deformed into abnormal, bubble-like structures. The length of the intestinal microvilli is decreased in these animals. However, the overall actin protein levels remain relatively unchanged when CCT is depleted. We also found that CCT depletion causes a reduction in the tubulin levels and disorganization of the microtubule network. In contrast, the stability and localization of intermediate filament protein IFB-2, which forms a dense filamentous network underneath the apical surface, appears to be superficially normal in CCT-deficient cells, suggesting substrate specificity of CCT in the folding of filamentous cytoskeletons in vivo. Our findings demonstrate physiological functions of CCT in epithelial cell morphogenesis using whole animals.

scholarly journals Crosstalk Between Nuclear Glucose-Regulated Protein 78 and Tumor Protein 53 Contributes to the Lipopolysaccharide Aggravated Apoptosis of Endoplasmic Reticulum Stress-Responsive Porcine Intestinal Epithelial Cells

2018 ◽  
Vol 48 (6) ◽  
pp. 2441-2455 ◽  
Author(s):  
Qian Jiang ◽  
Gang Liu ◽  
Jiashun Chen ◽  
Kang Yao ◽  
Yulong Yin
Keyword(s):  
Endoplasmic Reticulum ◽  
Epithelial Cells ◽  
Endoplasmic Reticulum Stress ◽  
Intestinal Inflammation ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Gram Negative ◽  
Rna Transfection ◽  
Glucose Regulated Protein

Background/Aims: Lipopolysaccharides (LPSs) act as virulence factors that trigger intestinal inflammation and thereby compromise the production of pigs worldwide. Intestinal diseases and dysfunction have been attributed to endoplasmic reticulum stress (ERS) and the subsequent apoptosis of intestinal epithelial cells. Therefore It is important to explore whether LPSs aggravate ERS-mediated apoptosis of intestinal epithelial cells. Methods: ERS and inflammation models were established in porcine cell line J2 (IPEC-J2) and the cells were treated with tunicamycin or LPS at specific times. The expression of marker proteins was determined by western blot and immunofluorescence. Possible crosstalk between proteins was analyzed by co-immunoprecipitation. Small interfering RNA transfection was employed to verify the mechanisms. Results: We found that Escherichia coli-derived LPS aggravated ERS and ERS-mediated apoptosis in ERS-responsive IPEC-J2 cells. The crosstalk between nuclear glucose-regulated protein 78 (GRP78) and tumor protein 53 (p53) was verified to trigger this LPS-aggravated apoptosis of ERS-responsive intestinal cells. Conclusion: This novel finding implies that intestinal malfunctions might solely originate from the effects of Gram-negative bacteria on ERS-responsive intestinal cells. The regulation of ERS signaling (especially the crosstalk between nuclear GRP78 and p53) in ERS-responsive/rapidly growing intestines may help intestinal cells survive from Gram-negative bacterial infections.

scholarly journals A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cells

Lab on a Chip ◽  
2018 ◽  
Vol 18 (14) ◽  
pp. 2036-2046 ◽  
Author(s):  
Huanhuan Joyce Chen ◽  
Paula Miller ◽  
Michael L. Shuler
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Intestinal Epithelial Cells ◽  
3D Culture ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
Improved Model ◽  
Drug Studies ◽  
Human Intestinal Cells

A pumpless GI–Liver system using primary human intestinal epithelial cells serves as an improved model for drug studies.

scholarly journals Lactobacillus casei DN-114 001 Inhibits the Ability of Adherent-Invasive Escherichia coli Isolated from Crohn's Disease Patients To Adhere to and To Invade Intestinal Epithelial Cells

2005 ◽  
Vol 71 (6) ◽  
pp. 2880-2887 ◽  
Author(s):  
Isabelle Ingrassia ◽  
Antony Leplingard ◽  
Arlette Darfeuille-Michaud
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Epithelial Cells ◽  
Lactobacillus Casei ◽  
Intestinal Epithelial Cells ◽  
Probiotic Strain ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Inhibitory Effects ◽  
E Coli

ABSTRACT Ileal lesions in 36.4% of patients with Crohn's disease are colonized by pathogenic adherent-invasive Escherichia coli. The aim of this study was to determine the in vitro inhibitory effects of the probiotic strain, Lactobacillus casei DN-114 001, on adhesion to and invasion of human intestinal epithelial cells by adherent-invasive E. coli isolated from Crohn's disease patients. The experiments were performed with undifferentiated Intestine-407 cells and with undifferentiated or differentiated Caco-2 intestinal epithelial cells. Bacterial adhesion to and invasion of intestinal epithelial cells were assessed by counting CFU. The inhibitory effects of L. casei were determined after coincubation with adherent-invasive E. coli or after preincubation of intestinal cells with L. casei prior to infection with adherent-invasive E. coli. Inhibitory effects of L. casei on adherent-invasive E. coli adhesion to differentiated and undifferentiated intestinal epithelial cells reached 75% to 84% in coincubation and 43% to 62% in preincubation experiments, according to the cell lines used. Addition of L. casei culture supernatant to the incubation medium increased L. casei adhesion to intestinal epithelial cells and enhanced the inhibitory effects of L. casei. The inhibitory effects on E. coli invasion paralleled those on adhesion. This effect was not due to a bactericidal effect on adherent-invasive E. coli or to a cytotoxic effect on epithelial intestinal cells. As Lactobacillus casei DN-114 001 strongly inhibits interaction of adherent-invasive E. coli with intestinal epithelial cells, this finding suggests that the probiotic strain could be of therapeutic value in Crohn's disease.

scholarly journals Cell Surface-Associated Elongation Factor Tu Mediates the Attachment of Lactobacillus johnsonii NCC533 (La1) to Human Intestinal Cells and Mucins

2004 ◽  
Vol 72 (4) ◽  
pp. 2160-2169 ◽  
Author(s):  
Dominique Granato ◽  
Gabriela E. Bergonzelli ◽  
Raymond David Pridmore ◽  
Laure Marvin ◽  
Martine Rouvet ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Binding Capacity ◽  
Elongation Factor ◽  
Mass Spectrometry Analysis ◽  
Intestinal Cells ◽  
Elongation Factor Tu ◽  
Intestinal Epithelial ◽  
Lactobacillus Johnsonii ◽  
Proinflammatory Response

ABSTRACT The aim of this work was to identify Lactobacillus johnsonii NCC533 (La1) surface molecules mediating attachment to intestinal epithelial cells and mucins. Incubation of Caco-2 intestinal epithelial cells with an L. johnsonii La1 cell wall extract led to the recognition of elongation factor Tu (EF-Tu) as a novel La1 adhesin-like factor. The presence of EF-Tu at the surface of La1 was confirmed by analysis of purified outer surface protein extract by immunoblotting experiments, by electron microscopy, and by enzyme-linked immunosorbent assays of live bacteria. Furthermore, tandem mass spectrometry analysis proved that EF-TU was expressed at the La1 surface as an intact molecule. Using recombinant La1 EF-Tu protein, we were able to determine that its binding to intestinal cells and to mucins is pH dependent. Competition experiments suggested that EF-Tu has an important role in La1 mucin binding capacity. In addition, immunomodulation studies performed on HT29 cells showed that EF-Tu recombinant protein can induce a proinflammatory response in the presence of soluble CD14. Our in vitro results indicate that EF-Tu, through its binding to the intestinal mucosa, might participate in gut homeostasis.

scholarly journals The food additive E171 and titanium dioxide nanoparticles indirectly alter the homeostasis of human intestinal epithelial cells in vitro

2019 ◽  
Vol 6 (5) ◽  
pp. 1549-1561 ◽  
Author(s):  
Marie Dorier ◽  
David Béal ◽  
Céline Tisseyre ◽  
Caroline Marie-Desvergne ◽  
Muriel Dubosson ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Titanium Dioxide Nanoparticles ◽  
Food Additive ◽  
Repeated Exposure ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

Repeated exposure to E171 or TiO2-NPs, in vitro, induce moderate inflammation and mucus secretion in intestinal cells.

Primary culture of salamander intestinal epithelial cells from nests: whole cell Na+ currents induced by valine

1994 ◽  
Vol 267 (1) ◽  
pp. G59-G66
Author(s):  
J. F. White
Keyword(s):  
Epithelial Cells ◽  
Intestinal Mucosa ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cells ◽  
Small Intestinal Mucosa ◽  
Intestinal Epithelial ◽  
Patch Clamp Technique ◽  
Whole Cell ◽  
Inward Current ◽  
Small Intestinal

Methods are described for isolating the cell nests, subepithelial clusters of germinative cells, from salamander intestinal mucosa and for growing the nests in culture into polarized monolayers of intestinal epithelial cells. Cells were viable in culture for up to 3 wk. The capacity of the monolayer cells to engage in membrane transport was evaluated using the patch-clamp technique in the whole cell mode. L-Valine (25 mM) induced an inward current in small intestinal cells of 25.8 +/- 5.7 pA and depolarized the cell membrane 14.5 +/- 1.6 mV. L-Alanine and L-phenylalanine were similarly effective, whereas D-valine was ineffective. The Km of the transporter for valine was 90 mM. Replacement of bath Na with tris(hydroxymethyl)aminomethane eliminated the inward current induced by valine. The basal (solute-independent) inward current was also reduced by Na+ replacement. Glucose did not induce a Na+ current. In contrast to the effect of valine on small intestinal cells, large intestinal cells were unresponsive to valine. It is concluded that the cultured small intestinal cells possess Na-amino acid but not Na-sugar cotransport. This profile of behavior is characteristic of undifferentiated small intestinal cells. Primary cultures of salamander small intestinal cells should be useful for studying enterocyte function and the developmental biology of the small intestinal mucosa.

scholarly journals Lactobacillus Reuteri grows in the Microalga Isochrysis Galbana Generating a Fermented Compound that reduces the AIEC Bacteria Harmfulness

2021 ◽  
Author(s):  
E. Colantoni ◽  
F. Palone ◽  
V. Cesi ◽  
B. Leter ◽  
G. Sugoni ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Health ◽  
Culture Medium ◽  
Lactobacillus Reuteri ◽  
Intestinal Epithelial Cells ◽  
Isochrysis Galbana ◽  
Anaerobic Growth ◽  
Intestinal Epithelial ◽  
Sustainable Resources

Abstract Microalgae are currently considered as alternative sustainable resources for high-value bioproducts such as omega3 polyunsaturated fatty acids (PUFAs). Probiotics are assumed to benefit human health by their direct actions on the composition and function of gut microbiota. Aims of the study are: 1) to set up the anaerobic growth of the probiotic Lactobacillus reuteri (L. reuteri) in the omega3-rich microalga Isochrysis galbana (I. galbana); 2) to assess the potential role of the obtained fermented compound (FC) to control the harmfulness of adherent invasive Escherichia coli (AIEC) to intestinal epithelial cells. I. galbana powder solubilized in PBS was used for the anaerobic growth of L. reuteri. The lipidic content of I. galbana and FC was analyzed by GC-MS. Colorectal adenocarcinoma cells CACO2 and the AIEC strain LF82 were used for in vitro experiments. I. galbana is shown to be an excellent culture medium for growing L. reuteri. The obtained FC significantly reduces the AIEC adhesiveness and invasiveness to intestinal epithelial cells. We show for the first time that microalgae may represent an innovative culture medium to grow probiotics in anaerobiosis. The obtained FC shows beneficial properties for human health by controlling the harmfulness of AIEC bacteria.

scholarly journals Association of Protease Activity in Vibrio choleraeVaccine Strains with Decreases in Transcellular Epithelial Resistance of Polarized T84 Intestinal Epithelial Cells

2000 ◽  
Vol 68 (11) ◽  
pp. 6487-6492 ◽  
Author(s):  
Stephanie F. Mel ◽  
Karla Jean Fullner ◽  
Susan Wimer-Mackin ◽  
Wayne I. Lencer ◽  
John J. Mekalanos
Keyword(s):  
Epithelial Cells ◽  
Vibrio Cholerae ◽  
Protease Activity ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Culture Supernatants ◽  
Epithelial Resistance

ABSTRACT Culture supernatants prepared from reactogenic strains ofVibrio cholerae cause a decrease in the transcellular epithelial resistance of T84 intestinal cells. This decrease correlates with the presence of hemagglutinin/protease but not with the presence of other potential accessory toxins or proteases. These data suggest a possible role for hemagglutinin/protease in reactogenicity, although other factors may also contribute.

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