Ultrasensitive version of nucleic acid sequence-based amplification (NASBA) utilizing nicking and extension chain reaction system

Nanoscale ◽  
2021 ◽  
Author(s):  
Yong Ju ◽  
Hyo Yong Kim ◽  
Jun Ki Ahn ◽  
Hyun Gyu Park
Keyword(s):  
Nucleic Acid ◽  
Reaction System ◽  
Isothermal Amplification ◽  
Nucleic Acid Sequence ◽  
Chain Reaction ◽  
Rna Targets ◽  
System P ◽  
Amplification Technique ◽  
Extension Chain

Nucleic acid sequence-based amplification (NASBA) is a transcription-based isothermal amplification technique especially designed for the detection of RNA targets. The NASBA basically relies on the linear production of T7 RNA...

scholarly journals Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 76
Author(s):  
Faiz Padzil ◽  
Abdul Razak Mariatulqabtiah ◽  
Wen Siang Tan ◽  
Kok Lian Ho ◽  
Nurulfiza Mat Isa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Isothermal Amplification ◽  
Screening Methods ◽  
Diagnostic Strategy ◽  
Chain Reaction ◽  
Loop Mediated Isothermal Amplification ◽  
Polymerase Chain

Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic acid screening methods such as polymerase chain reaction (PCR), qualitative (real-time) polymerase chain reaction (qPCR) and reverse-transcription qPCR (RT-qPCR). While such molecular detection methods are widely utilized as the benchmark, the invention of isothermal amplifications has also emerged as a reliable tool to improvise on-field diagnosis without dependence on thermocyclers. Among the established isothermal amplification technologies are loop-mediated isothermal amplification (LAMP), recombinant polymerase amplification (RPA), strand displacement activity (SDA), nucleic acid sequence-based amplification (NASBA), helicase-dependent amplification (HDA) and rolling circle amplification (RCA). This review highlights the past research on and future prospects of LAMP, its principles and applications as a promising point-of-care diagnostic method against avian viruses.

PrimRglo: A multiplexable quantitative real-time polymerase chain reaction system for nucleic acid detection

2012 ◽  
Vol 422 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Richard Lai ◽  
Fang Liang ◽  
Darnley Pearson ◽  
Graeme Barnett ◽  
David Whiley ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Reaction System ◽  
Nucleic Acid Detection ◽  
Chain Reaction ◽  
Polymerase Chain Reaction System ◽  
Polymerase Chain

Unexpected detection of DNA by nucleic acid sequence-based amplification technique

2004 ◽  
Vol 18 (4) ◽  
pp. 251-253 ◽  
Author(s):  
David Rodrı́guez-Làzaro ◽  
Joy Lloyd ◽  
John Ikonomopoulos ◽  
Maria Pla ◽  
Nigel Cook
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Sequence ◽  
Amplification Technique
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