Saltatory Rolling Circle Amplification (SRCA): a Novel Nucleic Acid Isothermal Amplification Technique Applied for Rapid Detection of Shigella Spp. in Vegetable Salad

2017 ◽  
Vol 11 (2) ◽  
pp. 504-513 ◽  
Author(s):  
Zhiyan Wang ◽  
Qian Yang ◽  
Yunzhe Zhang ◽  
Zhaoxiang Meng ◽  
Xiaoyan Ma ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rapid Detection ◽  
Rolling Circle Amplification ◽  
Isothermal Amplification ◽  
Rolling Circle ◽  
Shigella Spp ◽  
Amplification Technique

A netlike rolling circle nucleic acid amplification technique

The Analyst ◽  
2015 ◽  
Vol 140 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Xiaoli Zhu ◽  
Chang Feng ◽  
Bin Zhang ◽  
Hui Tong ◽  
Tao Gao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Nucleic Acid Amplification ◽  
Amplification Efficiency ◽  
Rolling Circle ◽  
Isothermal Nucleic Acid Amplification ◽  
Amplification Technique ◽  
Nucleic Acid Amplification Technique ◽  
Network Morphology

An isothermal nucleic acid amplification technique termed as netlike rolling circle amplification is proposed. Dense and uniform network morphology of amplified products is first observed, suggesting the ultrahigh amplification efficiency.

scholarly journals Strategies for Isothermal Amplification of Nucleic Acids: are they Ready to be Applied in Point of Care Diagnosis of Mycosis?

2020 ◽  
Vol 11 (3) ◽  
pp. 10559-10571
Keyword(s):  
Nucleic Acid ◽  
Clinical Laboratory ◽  
Multiple Displacement Amplification ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Isothermal Amplification ◽  
Nucleic Acid Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Isothermal Nucleic Acid Amplification

The early detection of invasive fungal infection (IFD) is significant in order to decrease mortality in susceptible patients. There is, therefore, a need for sensitive and specific fungal species detection assays in a clinical laboratory for early targeted therapy. The isothermal amplification method may be useful for the screening of fungal isolates, especially in resource-poor settings. Therefore, our aim was to review the isothermal nucleic acid amplification methods and their applications in fungal pathogen detection. Out of 50 reported studies, 28, 12, 6, 2, and 2 studies used the isothermal-based assays of a loop-mediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASBA), rolling circle amplification (RCA), multiple displacement amplification (MDA) and polymerase Spiral Reaction (PSR), respectively. Thirty-two studies used clinical samples, 18 pure culture, and four environmental samples. The diagnostic accuracy of isothermal nucleic acid amplification testing for pathogenic fungal was reported as high (sensitivity 0.89–1.0 and specificity 0.63–1.0) in all studies irrespective of the sample tested. Although the isothermal-based assays showed high sensitivity and specificity in reported studies, it is still poorer than that of PCR assays. However, improving the assay to make it simpler, more effective, and inexpensive compared with newer PCR methods are still needed.

Signal-on electrochemical DNA (E-DNA) sensor for accurate quantification of nicking-assisted rolling circle amplification (N-RCA) products with attomolar sensitivity

2021 ◽  
Author(s):  
Mengmeng Li ◽  
Dandan Li ◽  
Guidan Huang ◽  
Linying Zhou ◽  
Qilin Wen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Diagnosis ◽  
Rolling Circle Amplification ◽  
High Specificity ◽  
Dna Sensor ◽  
Rolling Circle ◽  
Specificity And Sensitivity ◽  
Nucleic Acid Analysis ◽  
Amplification Technique ◽  
Accurate Quantification

Rolling circle amplification (RCA) has become increasingly important amplification technique in nucleic acid analysis, immunoassay, and molecular diagnosis due to its high specificity and sensitivity. However, the accurate quantification of...

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

The analysis of proteins and small molecules based on sterically tunable nucleic acid hyperbranched rolling circle amplification

2017 ◽  
Vol 91 ◽  
pp. 136-142 ◽  
Author(s):  
Hai Shi ◽  
Xiaoxia Mao ◽  
Xiaoxia Chen ◽  
Zihan Wang ◽  
Keming Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Small Molecules ◽  
Rolling Circle Amplification ◽  
Rolling Circle

Mitigating the Non-Specific Amplification in RCA: Assessment of the Role of Ligation and Exonuclease Digestion in Circular DNA Preparation

2021 ◽  
Author(s):  
Vandana Kuttappan Nair ◽  
Chandrika Sharma ◽  
Mrittika Sengupta ◽  
Souradyuti Ghosh
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
False Positive ◽  
Rolling Circle Amplification ◽  
Circular Dna ◽  
Rolling Circle ◽  
Specific Amplification ◽  
Exonuclease Digestion ◽  
Sticky End

<b>Layman Summary: </b>Rolling circle amplification (RCA) is a popular and extensively used bioanalytical tool. Like any nucleic acid amplifications, non-specific amplification may occur in it and risk generating false positive readouts. The work described in the manuscript investigates non-specific amplification in RCA as a function of ligation and exonuclease digestion assays during the synthesis of circular DNA. In particular, it investigates and compares the role of three different ligation techniques, namely splint-padlock ligation, cohesive end (sticky end ligation), and self-annealing ligation. In addition, it also probes the role of single exonuclease vs dual exonuclease digestions. We employed real time fluorescence to quantify the effect of these factors. Finally, our work hypothesizes the possible origins of non-specific amplification in RCA.

Ultrasensitive version of nucleic acid sequence-based amplification (NASBA) utilizing nicking and extension chain reaction system

Nanoscale ◽  
2021 ◽  
Author(s):  
Yong Ju ◽  
Hyo Yong Kim ◽  
Jun Ki Ahn ◽  
Hyun Gyu Park
Keyword(s):  
Nucleic Acid ◽  
Reaction System ◽  
Isothermal Amplification ◽  
Nucleic Acid Sequence ◽  
Chain Reaction ◽  
Rna Targets ◽  
System P ◽  
Amplification Technique ◽  
Extension Chain

Nucleic acid sequence-based amplification (NASBA) is a transcription-based isothermal amplification technique especially designed for the detection of RNA targets. The NASBA basically relies on the linear production of T7 RNA...

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