scholarly journals Widefield phototransient imaging for visualizing 3D motion of resonant particles in scattering environments

Nanoscale ◽  
2022 ◽  
Author(s):  
Matz Liebel ◽  
Franco V. A. Camargo ◽  
Giulio Cerullo ◽  
Niek F. van Hulst

We show how ultrafast holographic transient microscopy can be used to identify, visualise and 3D track dynamically moving non-fluorescent nanoparticles in large volumes-of-view and in the presence of non-specific scattering background.

Author(s):  
Jonathan Kenneth Sinclair ◽  
Lindsay Bottoms

AbstractRecent epidemiological analyses in fencing have shown that injuries and pain linked specifically to fencing training/competition were evident in 92.8% of fencers. Specifically the prevalence of Achilles tendon pathology has increased substantially in recent years, and males have been identified as being at greater risk of Achilles tendon injury compared to their female counterparts. This study aimed to examine gender differences in Achilles tendon loading during the fencing lunge.Achilles tendon load was obtained from eight male and eight female club level epee fencers using a 3D motion capture system and force platform information as they completed simulated lunges. Independent t-tests were performed on the data to determine whether differences existed.The results show that males were associated with significantly greater Achilles tendon loading rates in comparison to females.This suggests that male fencers may be at greater risk from Achilles tendon pathology as a function of fencing training/ competition.


2020 ◽  
Vol 17 ◽  
Author(s):  
Dilawar Hassan ◽  
Hadi Bakhsh ◽  
Asif M. Khurram ◽  
Shakeel A. Bhutto ◽  
Nida S. Jalbani ◽  
...  

Background: The optical properties of nanomaterials have evolved enormously with the introduction of nanotechnology. The property of materials to absorb and/or emit specific wavelength has turned them into one of the most favourite candidates to be effectively utilized in different sensing applications e.g organic light emission diodes (OLEDs) sensors, gas sensors, biosensors and fluorescent sensors. These materials have been reported as a sensor in the field of tissue and cell imaging, cancer detection and detection of environmental contaminants etc. Fluorescent nanomaterials are heling in rapid and timely detection of various contaminants that greatly impact the quality of life and food, that is exposed to these contaminants. Later, all the contaminants have been investigated to be most perilous entities that momentously affect the life span of the animals and humans who use those foods which have been contaminated. Objective: In this review, we will discuss about various methods and approaches to synthesize the fluorescent nanoparticles and quantum dots (QDs) and their applications in various fields. The application will include the detection of various environmental contaminants and bio-medical applications. We will discuss the possible mode of action of the nanoparticles when used as sensor for the environmental contaminants as well as the surface modification of some fluorescent nanomaterials with anti-body and enzyme for specific detection in animal kingdom. We will also describe some RAMAN based sensors as well as some optical sensing-based nanosensors. Conclusion: Nanotechnology has enabled to play with the size, shape and morphology of materials in the nanoscale. The physical, chemical and optical properties of materials change dramatically when they are reduced to nanoscale. The optical properties can become choosy in terms of emission or absorption of wavelength in the size range and can result in production of very sensitive optical sensor. The results show that the use of fluorescent nanomaterials for the sensing purposes are helping a great deal in the sensing field.


Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.


2021 ◽  
Author(s):  
Leah M. Johnson ◽  
Jeffrey B. Mecham ◽  
Sai Archana Krovi ◽  
Maria M. Moreno Caffaro ◽  
Shyam Aravamudhan ◽  
...  

Fluorescent nanoparticles (NPs) comprising polyethylene terephthalate (PET) with a hydrodynamic diameter of 158 ± 2 nm were synthesized in a bottom-up approach.


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Gordon J. Hedley ◽  
Tim Schröder ◽  
Florian Steiner ◽  
Theresa Eder ◽  
Felix J. Hofmann ◽  
...  

AbstractThe particle-like nature of light becomes evident in the photon statistics of fluorescence from single quantum systems as photon antibunching. In multichromophoric systems, exciton diffusion and subsequent annihilation occurs. These processes also yield photon antibunching but cannot be interpreted reliably. Here we develop picosecond time-resolved antibunching to identify and decode such processes. We use this method to measure the true number of chromophores on well-defined multichromophoric DNA-origami structures, and precisely determine the distance-dependent rates of annihilation between excitons. Further, this allows us to measure exciton diffusion in mesoscopic H- and J-type conjugated-polymer aggregates. We distinguish between one-dimensional intra-chain and three-dimensional inter-chain exciton diffusion at different times after excitation and determine the disorder-dependent diffusion lengths. Our method provides a powerful lens through which excitons can be studied at the single-particle level, enabling the rational design of improved excitonic probes such as ultra-bright fluorescent nanoparticles and materials for optoelectronic devices.


2014 ◽  
Vol 7 (S1) ◽  
Author(s):  
Se won Yoon ◽  
Jeong woo Lee ◽  
Soo ji Park ◽  
Woong sik Park ◽  
Moon jeong Kim

2021 ◽  
Vol 355 ◽  
pp. 109108
Author(s):  
Manish Anand ◽  
Jed A. Diekfuss ◽  
Alexis B. Slutsky-Ganesh ◽  
Dustin R. Grooms ◽  
Scott Bonnette ◽  
...  

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