scholarly journals Subcellular structure of bovine thyroid gland. The localization of the peroxidase activity in bovine thyroid

1978 ◽  
Vol 174 (3) ◽  
pp. 939-949 ◽  
Author(s):  
M J S De Wolf ◽  
A R Lagrou ◽  
H J J Hilderson
Keyword(s):  
Peroxidase Activity ◽  
Buoyant Density ◽  
Thyroid Tissue ◽  
Guaiacol Peroxidase ◽  
Subcellular Structure ◽  
The Third ◽  
Bovine Thyroid ◽  
Two Peaks ◽  
Second Peak ◽  
Membranous Material

1. After differential pelleting of bovine thyroid tissue the highest relative specific activities for plasma membrane markers are found in the L fraction whereas those for peroxidase activities (p-phenylenediamine, guaiacol and 3,3′-diaminobenizidine tetrachloride peroxidases) are found in the M fraction. 2. When M + L fractions were subjected to buoyant-density equilibration in a HS zonal rotor all peroxidases show different profiles. The guaiacol peroxidase activity always follows the distribution of glucose 6-phosphatase. 3. When a Sb fraction is subjected to Sepharose 2B chromatography three major peaks are obtained. The first, eluted at the void volume, consists of membranous material and contains most of the guaiacol peroxidase activity. Most of the protein (probably thyroglobulin) is eluted with the second peak. Solubilized enzymes are recovered in the third peak. 4. p-Phenylenediamine peroxidase activity penetrates into the gel on polyacrylamidegel electrophoresis, whereas guaiacol peroxidase activity remains at the sample zone. 5. DEAE-Sephadex A-50 chromatography resolves the peroxidase activities into two peaks, displaying different relative amounts of the different enzymic activities in each peak. 6. The peroxidase activities may be due to the presence of different proteins. A localization of guaiacol peroxidase in rough-endoplasmic-reticulum membranes (or in membranes related to them) seems very likely.

A study of thyroglobulin and peroxidase activity in the thyroid tissue of patients with non-endemic non-toxic nodular goitre

1991 ◽  
Vol 80 (4) ◽  
pp. 301-307 ◽  
Author(s):  
T. Mories ◽  
J. M. Miralles ◽  
A. Reglero ◽  
S. Felipe ◽  
J. J. Corrales ◽  
...  
Keyword(s):  
Peroxidase Activity ◽  
Thyroid Tissue ◽  
Enzymic Activity ◽  
Thyroid Peroxidase ◽  
Carbohydrate Composition ◽  
Nodular Goitre ◽  
Hormone Synthesis ◽  
Control Subjects ◽  
The Mean ◽  
Second Peak

1. A study was made of the activity of the enzyme thyroid peroxidase and of the concentration, carbohydrate composition and the degree of iodination of thyroglobulin in the thyroid glands of 60 patients with non-endemic non-toxic goitre in the nodular phase and in those of 25 control subjects. 2. Thyroid peroxidase activity was determined by the guaiacol assay and was significantly higher in patients with non-endemic non-toxic goitre than in control subjects (3.60 ± 2.51 and 2.07 ± 1.08 μmol of guaiacol oxidized min−1 g−1 of tissue, respectively; ranges 0.16–10.57 and 0.52–4.85 μmol of guaiacol oxidized min−1 g−1 of tissue, respectively; P <0.05). 3. Thyroglobulin was purified by precipitation with ammonium sulphate and Sephadex G-200 gel filtration. Two protein peaks were obtained which were identified as thyroglobulin and measured by radioimmunoassay. The concentration of thyroglobulin in the first peak was 98.94 (SD 84.87, range 0.60–455.54) mg/g of tissue for the patients with non-endemic, non-toxic goitre and 51.41 (SD 28.34, range 14.99–106.39) mg/g of tissue for the control subjects (P <0.01). The second peak showed 1.26 (SD 1.27, range 0.09–6.50) mg of thyroglobulin/mg of tissue for the group with non-endemic non-toxic goitre and 0.51 (SD 0.25, range 0.15–0.98 mg of thyroglobulin/mg of tissue) for the control subjects (P <0.01). 4. The carbohydrate composition of thyroglobulin was determined by acid hydrolysis and colorimetry, evaluating the levels of hexoses, hexosamines and sialic acid. The degree of glycosylation was significantly higher in thyroglobulin from patients with non-endemic non-toxic goitre (mean 16.46%, SD 5.68%, range 8.33–37.3% for the first peak, and mean 16.11%, SD 4.85%, range 5.30–33.10% for the second peak) than that from control subjects (mean 9.62%, SD 1.23%, range 7.56–12.02% for the first peak, and mean 8.68%, SD 0.99%, range 7.24–11.09% for the second peak). The values for each of the carbohydrates were significantly higher in the thyroglobulin from patients with non-endemic non-toxic goitre than in the control subjects, and there was also a positive correlation between them, except for the hexoses and hexosamines in the second peak of the thyroglobulin from the control subjects. 5. The thyroglobulin of the group with non-endemic non-toxic goitre was significantly less iodinated (mean 0.055%, SD 0.038%, range 0.021–0.161% in the first peak, and mean 0.30%, SD 0.22%, range 0.10–0.93% in the second peak) than that of the control subjects (mean 0.32%, SD 0.16%, range 0.07–0.64% in the first peak, and mean 0.77%, SD 0.17%, range 0.37–1.27% in the second peak). 6. A significant negative correlation was observed between the degree of thyroglobulin iodination and thyroid peroxidase activity in control subjects; however, this was not found in patients with non-endemic non-toxic goitre. The mean level of peroxidase activity in the latter group was 26.71% above what would be expected for the mean degree of thyroglobulin iodination found, indicating that other additional factors may govern the increase in enzymic activity. 7. Such findings point to the existence of an alteration in thyroglobulin that may decrease the efficiency of the protein for normal hormone synthesis, and this may be one of the reasons for the development and/or maintenance of non-endemic non-toxic goitre.

scholarly journals The study of multipeaked type-I X-ray bursts in the neutron star low-mass X-ray binary 4U 1636 − 536 with RXTE

2020 ◽  
Vol 501 (1) ◽  
pp. 168-178
Author(s):  
Chen Li ◽  
Guobao Zhang ◽  
Mariano Méndez ◽  
Jiancheng Wang ◽  
Ming Lyu
Keyword(s):  
Neutron Star ◽  
Flux Ratio ◽  
Light Curves ◽  
Type I ◽  
X Ray ◽  
Soft State ◽  
Peak Flux ◽  
Low Mass ◽  
Two Peaks ◽  
Second Peak

ABSTRACT We have found and analysed 16 multipeaked type-I bursts from the neutron-star low-mass X-ray binary 4U 1636 − 53 with the Rossi X-ray Timing Explorer (RXTE). One of the bursts is a rare quadruple-peaked burst that was not previously reported. All 16 bursts show a multipeaked structure not only in the X-ray light curves but also in the bolometric light curves. Most of the multipeaked bursts appear in observations during the transition from the hard to the soft state in the colour–colour diagram. We find an anticorrelation between the second peak flux and the separation time between two peaks. We also find that in the double-peaked bursts the peak-flux ratio and the temperature of the thermal component in the pre-burst spectra are correlated. This indicates that the double-peaked structure in the light curve of the bursts may be affected by enhanced accretion rate in the disc, or increased temperature of the neutron star.

Monoamine Oxidase in Bovine Thyroid Tissue

1986 ◽  
Vol 18 (11) ◽  
pp. 750-753 ◽  
Author(s):  
A. Masini-Repiso ◽  
A. Cabanillas ◽  
M. Andrada ◽  
A. Coleoni
Keyword(s):  
Monoamine Oxidase ◽  
Thyroid Tissue ◽  
Bovine Thyroid

scholarly journals Reflectance Spectrum of a Color Blue Generated by Curved Multilayer and Iridescent of the Elytron of the Calidea Signata Bug

2016 ◽  
Vol 12 (36) ◽  
pp. 1
Author(s):  
Issaka Ouedraogo ◽  
Serge Wendsida Igo ◽  
Priscilla Simonis ◽  
Alioune Ouedraogo ◽  
Belkacem Zeghmati
Keyword(s):  
Multilayer Structure ◽  
Reflectance Spectrum ◽  
Main Peak ◽  
Blue Color ◽  
Transfer Matrices ◽  
Microscopy Characterization ◽  
Two Peaks ◽  
Second Peak ◽  
Scanning Electron Microscopy Characterization ◽  
Scanning Electron

This paper focuses on the study of the origins of blue iridescent reflections of the elytron of Calidea Signata, of the pentatomidae family which is commonly known as bug. Indeed, we use the spectrophotometry measurements, scanning electron microscopy characterization technique, and a ray tracing code program combined with the transfer matrices method, to explain the structure which is responsible for the color of the blue reflections and the iridescent effect. Thus, we noticed that the color of the bug as a result of two peaks. The first and main peak is located at 485.5 nm, corresponding to the blue color. The second peak, is less intense at 525 nm, corresponding to the green-blue color. The numerical reproduction of the reflectance spectrum gives 510.25 nm for the main peak, and 539.5 nm for the secondary peak. These results confirm that the multilayer structure is responsible for the blue color of the Calidea Signata bug. Lastly, the curve of the multilayer is responsible for the iridescence.

scholarly journals The magnitude of variation in temperature within a year has an effect on the seasonal variations of chickenpox incidence in Japan

2001 ◽  
Vol 126 (2) ◽  
pp. 269-277 ◽  
Author(s):  
A. KOKAZE ◽  
M. YOSHIDA ◽  
Y. SEKINE ◽  
M. ISHIKAWA ◽  
T. KUROKOCHI ◽  
...  
Keyword(s):  
Seasonal Variations ◽  
Close Correlation ◽  
Daily Temperature ◽  
Size And Shape ◽  
Different Types ◽  
Close Relationship ◽  
The Difference ◽  
Two Peaks ◽  
Second Peak

We investigated the epidemic pattern of chickenpox incidence among 47 prefectures in Japan. There were two peaks in chickenpox incidence in all prefectures. The first peaks appear at almost the same time in a year, while the second peaks occur at different times with relatively different types of size and shape. The feature of the second peak might characterize the epidemic pattern of chickenpox. We first introduced the second peak index, that is, the ratio of the difference between the incidence at the point of the second peak and the minimum incidence between the first and second peaks to the difference between the incidence at the point of the second peak and the minimum incidence in the year. There was a close correlation between the second peak index and the magnitude of variation in temperature within a year corresponding to the difference between the maximum and the minimum of the monthly mean of the highest daily temperature. This is the first article focusing on the close relationship between the second peak of epidemic pattern of chickenpox incidence and the variation of temperature within a year.

scholarly journals Thyrotoxicosis and the consumption of bovine thyroid tissue

QJM ◽  
2020 ◽  
Author(s):  
S Senthilkumaran ◽  
F Benita ◽  
P Thirumalaikolundusubramanian ◽  
K Swaminathan
Keyword(s):  
Thyroid Tissue ◽  
Bovine Thyroid

Heat Transfer Measurements of Oblong Pins

2014 ◽  
Author(s):  
Kathryn L. Kirsch ◽  
Karen A. Thole
Keyword(s):  
Heat Transfer ◽  
Gas Turbine ◽  
Flow Direction ◽  
Effective Means ◽  
Transfer Enhancement ◽  
Pin Fin ◽  
The Third ◽  
Pin Fins ◽  
Two Peaks ◽  
Pin Fin Arrays

Pin fin arrays are employed as an effective means for heat transfer enhancement in the internal passages of a gas turbine blade, specifically in the blade’s trailing edge. Various shapes of the pin itself have been used in such arrays. In this study, oblong pin fins are investigated whereby their long axis is perpendicular to the flow direction. Heat transfer measurements were taken at the pin mid-span with unheated endwalls to isolate the pin heat transfer. Results show important differences in the heat transfer patterns between a pin in the first row and a pin in the third row. In the third row, wider spanwise spacing allows for two peaks in heat transfer over the pin surface. Additionally, closer streamwise spacing leads to consistently higher heat transfer for the same spanwise spacing. Due to the blunt orientation of the pins, the peak in heat transfer occurs off the stagnation point.

Relationship between Activities of Enzymes for the Removal of O2·- and H2O2 and Nitrogenase in Root Nodules of Phaseolus vulgaris L.

1995 ◽  
Vol 50 (7-8) ◽  
pp. 543-551
Author(s):  
Bernhard Epping ◽  
Alexander P. Hansen ◽  
Peter Martin
Keyword(s):  
Superoxide Dismutase ◽  
Phaseolus Vulgaris ◽  
Ascorbate Peroxidase ◽  
Peroxidase Activity ◽  
Rhizobium Leguminosarum ◽  
Nitrogenase Activity ◽  
Root Nodules ◽  
Hypersensitive Reaction ◽  
Guaiacol Peroxidase ◽  
Bacterial Strains

Abstract Nodules of Rhizobium leguminosarum bv. phaseoli in symbiosis with Phaseolus vulgaris were compared with regard to their nitrogenase activity and activities of enzymes involved in the removal of O2·- and H2O2 as well as total ascorbate content. Activities of catalase (EC 1.11.1.6), ascorbate peroxidase (EC 1.11.1.11), and total ascorbate content were consist­ently higher in nodules inhabited by bacterial strains with higher nitrogenase activity. Values for superoxide dismutase (EC 1.15.11), and guaiacol peroxidase activity did not differ for the bacterial strains compared. On the other hand, when different plant cultivars were inoculated with the same bacterial strain, high nitrogenase activity did not correlate with a higher activ­ity of the oxygen scavenging enzyms or a higher content of total ascorbate. In this case, values for guaiacol peroxidase activity were greatly enhanced in nodules with lower nitrogen­ ase activity. This may be part of a hypersensitive reaction of the plant cultivar against the bacterial symbiotic partner. Inhibition of catalase activity in the nodules by addition of triazole to the nutrient solution did not alter nitrogenase activity within the first nine hours after addition. It can be concluded that the activity of catalase, ascorbate peroxidase, and superoxide dismutase is not generally coupled to nitrogenase activity in root nodules of P. vulgaris.

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