scholarly journals Delineation of fucosyltransferase activities with thiol reagents

1979 ◽  
Vol 181 (3) ◽  
pp. 767-769 ◽  
Author(s):  
D Kessel ◽  
T H Chou
Keyword(s):  
Blood Cell ◽  
The Other ◽  
Terminal Galactose ◽  
Thiol Reagent

The thiol reagent dithiothreitol inhibits the activity of a core GDP-fucose-N-acetylglucosaminide alpha-6-fucosyltransferase in plasma and blood-cell homogenates, while promoting the activity of alpha-2- and alpha-3-fucosyltransferases. The latter enzymes catalyse transfer of fucose on to terminal galactose and subterminal N-acetylglucosamine residues respectively. A thiol-blocking reagent N-ethylmaleimide does not affect the activity of the alpha-6-fucosyltransferase, but inhibits the other two enzymes. These results indicate the presence of a critical disulphide linkage in the alpha-6-fucosyltransferase, and provide a means of delineation of different fucosyltransferases.

scholarly journals Effects of dietary Mannan oligosaccharide (MOS) on grown, survival rate, intestinal morphology and blood cell count of the golden trevally fish (Gnathanodon specious)

2019 ◽  
Vol 19 (4A) ◽  
pp. 241-250
Author(s):  
Dang Tran Tu Tram ◽  
Nguyen Thi Nguyet Hue ◽  
Ho Son Lam ◽  
Nguyen Truong Tan Tai ◽  
Dao Thi Hong Ngoc
Keyword(s):  
Survival Rate ◽  
Blood Cell ◽  
Blood Cells ◽  
White Blood Cells ◽  
Intestinal Morphology ◽  
The Other ◽  
Control Group ◽  
Blood Cell Count ◽  
Mannan Oligosaccharide ◽  
Erythrocyte Density

The golden trevally fishes (Gnathanodon specious) (2.19 ± 0.23 g) were cultured in glass tanks with density of 20 fishes/tank and they were fed supplemental diets of different MOS concentrations (0; 0.2; 0.4 and 0.6%) for 90 days. Collected data included growth rate, survival rate and some hematological characteristics of this fish. The results demonstrated that MOS supplementation did not affect growth performance, erythrocyte density and blood cell size, however the survival rate was significantly increased. On the other hand, the total number of white blood cells (BC) on the 60th day in the fish fed with MOS supplements (5.78–6.96 × 104TB/mm3) was higher than that in the control group (only 5.43 × 104TB/mm3) with the largest total leukocytes (6.96 ± 0.50 × 104TB /mm3) at 0.2% MOS (p < 0.05).

Effect of the Red Blood Cells on the Primary Thrombus Formation

2007 ◽  
Author(s):  
Koichiro Yano ◽  
Daisuke Mori ◽  
Ken-ichi Tsubota ◽  
Takuji Ishikawa ◽  
Shigeo Wada ◽  
...  
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Blood Flow ◽  
Blood Cell ◽  
Blood Cells ◽  
Thrombus Formation ◽  
The Other ◽  
Biochemical Processes ◽  
Mechanical Factors ◽  
Molecular Bridge

It has been pointed out that some mechanical factors play important roles in a series of physiological or biochemical processes during the thrombus formation. Recently, many studies including the authors’ work qualitatively demonstrated how the thrombus is regulated under the influences of the blood flow and the intercellular molecular bridge using computational fluid dynamics techniques[1–4]. They verified the importance of the balance of them in the process of the thrombus formation. However, few studies have taken into account the existence of the other cell constituents than the platelet such as red blood cell (RBC).

scholarly journals Molecular Modelling of Human Red Blood Cell Pyruvate Kinase: Structural Implications of a Novel G1091 to A Mutation Causing Severe Nonspherocytic Hemolytic Anemia

Blood ◽  
1997 ◽  
Vol 90 (12) ◽  
pp. 4987-4995 ◽  
Author(s):  
Wouter W. van Solinge ◽  
Rob J. Kraaijenhagen ◽  
Gert Rijksen ◽  
Richard van Wijk ◽  
Bjarne B. Stoffer ◽  
...  
Keyword(s):  
Blood Cell ◽  
Pyruvate Kinase ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Molecular Modelling ◽  
Human Liver ◽  
The Other ◽  
Salt Bridges ◽  
Compound Heterozygotes ◽  
Allosteric Properties

Abstract We present a novel G1091 to A mutation in the human liver and red blood cell (RBC) pyruvate kinase (PK) gene causing severe hemolytic anemia. In two families, three children were severely PK-deficient compound heterozygotes exhibiting the G1091 to A mutation and a common G1529 to A mutation on the other allele. In one family, the mother, a G1091 to A heterozygote, later had a second baby with a new husband, also a G1091 to A carrier. The baby was homozygous for the G1091 to A mutation and died 6 weeks after birth from severe hemolysis. Both mutant alleles were expressed at the RNA level. The G1091 to A mutation results in the substitution of a conserved glycine by an aspartate in domain A of RBC PK, whereas the G1529 to A mutation leads to the substitution of a conserved arginine residue with glutamine in the C-domain. Molecular modelling of human RBC PK, based on the crystal structure of cat muscle PK, shows that both mutations are located outside the catalytic site at the interface of domains A and C. The mutations are likely to disrupt the critical conformation of the interface by introducing alternative salt bridges. In this way the Gly364 to Asp and Arg510 to Gln substitutions may cause PK deficiency by influencing the allosteric properties of the enzyme.

Evaluation of Platelet Concentrates with the New Dideco Blood Cell Separator: Excel

1993 ◽  
Vol 16 (5_suppl) ◽  
pp. 130-134 ◽  
Author(s):  
F. Schooneman
Keyword(s):  
Blood Cell ◽  
Platelet Activation ◽  
Complement Activation ◽  
The Other ◽  
Platelet Concentrates ◽  
Cell Separator ◽  
Good Compromise ◽  
Blood Cell Separator ◽  
The Future

The Excel Cell Separator produced by Dideco represents a good compromise between automation and Platelet efficiency. Platelet activation is a phenomenon that we can find with the concentrates of all cell separators. Although, the complement activation of Excel is extremely lower in comparison with the same data obtained using all the other cell separators, the 5-day storage is acceptable. It will be important to research the bacteriological controls. Like the pH of the platelet bags, because in certain cases it was too low. For the future it will be interesting to study the possibility to predict the quantity of platelets collected having a system that is adaptable with the donor parameters.

Studies in Red Blood Cell Preservation 1. Effect of the Other Formed Elements

Vox Sanguinis ◽  
1990 ◽  
Vol 58 (2) ◽  
pp. 85-89 ◽  
Author(s):  
T. J. Greenwalt ◽  
C. Zehner Sostok ◽  
U. J. Dumaswala
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
The Other ◽  
Cell Preservation

scholarly journals Incorporation and metabolism of exogenous GM1 ganglioside in rat liver

1986 ◽  
Vol 237 (1) ◽  
pp. 147-155 ◽  
Author(s):  
R Ghidoni ◽  
M Trinchera ◽  
B Venerando ◽  
A Fiorilli ◽  
S Sonnino ◽  
...  
Keyword(s):  
Rat Liver ◽  
Degradation Process ◽  
Major Product ◽  
The Other ◽  
Gm1 Ganglioside ◽  
Lysosomal Fraction ◽  
Long Chain Base ◽  
Terminal Galactose ◽  
Subcellular Level ◽  
Long Chain

The pathways of metabolic processing of exogenously administered GM1 ganglioside in rat liver was investigated at the subcellular level. The GM1 used was 3H-labelled at the level of long-chain base ([Sph(sphingosine)-3H]GM1) or of terminal galactose ([Gal-3H]GM1). The following radioactive compounds, derived from exogenous GM1, were isolated and chemically characterized: gangliosides GM2, GM3, GD1a and GD1b (nomenclature of Svennerholm [(1964) J. Lipid Res. 5, 145-155] and IUPAC-IUB Recommendations [(1977) Lipids 12, 455-468]); lactosylceramide, glucosylceramide and ceramide; sphingomyelin. GM2, GM3, lactosylceramide, glucosylceramide and ceramide, relatively more abundant shortly after GM1 administration, were mainly present in the lysosomal fraction and reflected the occurrence of a degradation process. 3H2O was also produced in relevant amounts, indicating complete degradation of GM1, although no free long-chain bases could be detected. GD1a and GD1b, relatively more abundant later on after administration, were preponderant in the Golgi-apparatus fraction and originated from a biosynthetic process. More GD1a was produced starting from [Sph-3H]GM1 than from [Gal-3H]GM1, and radioactive GD1b was present only after [Sph-3H]GM1 injection. This indicates the use of two biosynthetic routes, one starting from a by-product of GM1 degradation, the other implicating direct sialylation of GM1. Both routes were used to produce GD1a, but only the first one for producing GD1b. Sphingomyelin was the major product of GM1 processing, especially at the longer times after injection, and arose from a by-product of GM1 degradation, most likely ceramide.

Studies in Red Blood Cell Preservation - 1. Effect of the Other Formed Elements

Vox Sanguinis ◽  
1990 ◽  
Vol 58 (2) ◽  
pp. 85-89
Author(s):  
C. Zehner Sostok ◽  
U.J. Dumaswala ◽  
T.J. Greenwalt
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
The Other ◽  
Cell Preservation

Physiological effects of deuterium on dogs

1961 ◽  
Vol 201 (2) ◽  
pp. 357-362 ◽  
Author(s):  
Dorice M. Czajka ◽  
Asher J. Finkel ◽  
Conrad S. Fischer ◽  
Joseph J. Katz
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Deuterium Oxide ◽  
Myocardial Damage ◽  
Serum Glucose ◽  
The Body ◽  
The Other ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Blood Cell Count

The physiological consequences of the deuterium isotope effect in large mammals were studied in two dogs, one of which was maintained at 20% concentration of D2O in the body fluids for 50 days, and the other at the toxic range of 33–35% for a brief period. Deuteration of the dogs was effected by replacement of ordinary water with deuterium oxide in both food and drink. Hemoglobin, hematocrit, and red blood cell count dropped but the white blood cell count was essentially unaffected although there was a progressive lymphopenia and granulocytosis. Serum glucose was decreased, especially at higher deuterium levels. Total serum cholesterol values were also diminished although the esters were essentially unchanged. Serum sodium and both NPN and BUN were within normal limits except for a terminal elevation of the latter. Serum potassium was slightly lowered for a brief period after 3 weeks. Electrocardiograms showed ST segment coving and elevation and an increase in the QT ratio that suggested nonspecific myocardial damage; these changes reverted to normal while the dog was still deuterated at a level of 20%. Both dogs exhibited neuromuscular disturbances, in one case definite weakness of the hind legs and in the other, fine muscle tremors.

scholarly journals IV. On the structure of the red blood-corpuscle of oviparous vertebrata

1869 ◽  
Vol 17 ◽  
pp. 346-350
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
The Other ◽  
Red Cell ◽  
Blood Corpuscle ◽  
Fundamental Distinction ◽  
Long Time ◽  
History Of

The red blood-cell has been perhaps more frequently and fully examined than any other animal structure; certainly none has evoked such various and even contradictory opinions of its nature. But without attempting here any history of these, it may be shortly said that amongst the conclusions now, and for a long time past, generally accepted, a chief one is that a fundamental distinction exists between the red corpuscle of Mammalia and that of the other vertebrate classes—that the red cell of the oviparous vertebrata possesses a nucleus which is not to be found in the corpuscle of the other class. This great distinction between the classes has of late years been over and over again laid down in the strongest and most unqualified terms. But I venture to ask for a still further examination of this important subject.

Presentation patterns and prognosis of 109 isolated venous injuries in 99 patients

2019 ◽  
Vol 34 (10) ◽  
pp. 698-706 ◽  
Author(s):  
Stavros K Kakkos ◽  
Ioannis A Tsolakis ◽  
George Markopoulos ◽  
Ioannis Maroulis ◽  
Efstratios Koletsis ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Odds Ratio ◽  
The Other ◽  
Outcome Predictors ◽  
Iatrogenic Injuries ◽  
Body Retention ◽  
All Cause Mortality ◽  
External Causes ◽  
Related Mortality

Objectives To identify outcome predictors of isolated venous injuries (VIs). Methods Retrospective analysis of prospectively collected information. Results A total of 99 patients with 109 isolated VI were included. All-cause mortality was 18/99 (18%) and mortality related to the VI was 10/99 (10%). On multivariate analysis, independent predictors of all-cause mortality included age (odds ratio – OR – 1.06, p = 0.042), external cause – trauma and foreign body retention – of VI (OR 34.62, p = 0.002) and the number of red blood cell units transfused intraoperatively (OR 2.10, p < 0.001), while independent predictors of VI-related mortality included external cause of VI (OR 47.60, p = 0.001) and the number of red blood cell units transfused intraoperatively (OR 1.72, p = 0.003). Conclusions VIs due to external causes have a high mortality rate. On the other hand, VIs due to internal causes (iatrogenic injuries during a surgical procedure) are managed promptly and have a very low mortality related to the VI.

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