The SXFEL Upgrade: From Test Facility to User Facility

The Shanghai soft X-ray Free-Electron Laser facility (SXFEL), which is the first X-ray FEL facility in China, is being constructed in two phases: the test facility (SXFEL-TF) and the user facility (SXFEL-UF). The test facility was initiated in 2006 and funded in 2014. The commissioning of the test facility was finished in 2020. The user facility was funded in 2016 to upgrade the accelerator energy and build two undulator lines with five experimental end-stations. The output photon energy of the user facility will cover the whole water window range. This paper presents an overview of the SXFEL facility, including considerations of the upgrade, layout and design, construction status, commissioning progress and future plans.

The Levantine Intermediate Water in the western Mediterranean and its interactions with the Algerian Gyres: insights from 60 years of observation

The presence of two large scale cyclonic gyres in the Algerian basin influences the general and eddy circulation, but their effect on water mass transfer remain poorly characterized. Our study has confirmed the presence of these gyres using the first direct current measurements of the whole water column collected during the SOMBA-GE2014 cruise, specifically designed to investigate these gyres. Using cruise sections and a climatology from 60 years of in situ measurements, we have also shown the effect of these gyres on the distribution at intermediate depth of Levantine Intermediate Water (LIW) with warmer (~0.15 °C) and saltier (~0.02 g.kg−1) characteristics in the Algerian basin than in the Provençal basin. The Algerian gyres also impact horizontal density gradients with sinking of the isopycnals at the gyres’ centres. Temporal cross-correlation of LIW potential temperature referenced to the signal observed south of Sardinia reveal timescale of transit of 4 months to get to the centre of the Algerian basin. The LIW temperature and salinity trends over various periods are estimated to: +0.0017 ± 0.0014 °C.year−1 and +0.0017 ± 0.0003 year−1 respectively over the 1960–2017 period, and accelerating to +0.059 ± 0.072 °C.year−1 and +0.013 ± 0.006 year−1 over the 2013–2017 period.

Coordinated Diel Gene Expression of Cyanobacteria and Their Microbiome

Diel rhythms have been well recognized in cyanobacterial metabolisms. However, whether this programmed activity of cyanobacteria could elicit coordinated diel gene expressions in microorganisms (microbiome) that co-occur with cyanobacteria and how such responses in turn impact cyanobacterial metabolism are unknown. To address these questions, a microcosm experiment was set up using Lake Erie water to compare the metatranscriptomic variations of Microcystis cells alone, the microbiome alone, and these two together (whole water) over two day-night cycles. A total of 1205 Microcystis genes and 4779 microbiome genes exhibited significant diel expression patterns in the whole-water microcosm. However, when Microcystis and the microbiome were separated, only 515 Microcystis genes showed diel expression patterns. A significant structural change was not observed for the microbiome communities between the whole-water and microbiome microcosms. Correlation analyses further showed that diel expressions of carbon, nitrogen, phosphorous, and micronutrient (iron and vitamin B12) metabolizing genes were significantly coordinated between Microcystis and the microbiome in the whole-water microcosm. Our results suggest that diel fluxes of organic carbon and vitamin B12 (cobalamin) in Microcystis could cause the diel expression of microbiome genes. Meanwhile, the microbiome communities may support the growth of Microcystis by supplying them with recycled nutrients, but compete with Microcystis for iron.

A journey towards whole water certified reference materials for organic substances: measuring polycyclic aromatic hydrocarbons as required by the European Union Water Framework Directive

In 2000, the Water Framework Directive (WFD) came into force in the European Union with the aim of protecting and improving water quality. The priority substances established to be monitored are predominantly organic compounds, for which the WFD sets the requirement of ‘whole water sample’ analysis. This legislative requirement poses analytical challenges for the monitoring laboratories as well as technical challenges for reference materials producers. In the past, there were attempts to produce reference materials as quality assurance/quality control tools for measuring organic priority substances in whole water. A critical reflection on the approaches and solutions applied to prepare such kind of matrix reference materials is presented along with a discussion on the difficulties encountered by the analytical laboratories in analysing such complex matrices. The Certified Reference Material (CRM) ERM-CA100 can be considered as a pioneer for a ‘whole water’ CRM (containing humic acids) and has been designed for the analysis of polycyclic aromatic hydrocarbons (PAHs). Further developments seem to be necessary to upgrade the design towards a CRM which will also include suspended particulate matter, another basic constituent of natural surface water samples. Graphical abstract

Study of the thermal regime of a reservoir on the Qinghai-Tibetan Plateau, China

The Qinghai-Tibetan Plateau region has unique meteorological characteristics, with low air temperature, low air pressure, low humidity, little precipitation, and strong diurnal variation. A two-dimensional hydrodynamic CE-QUAL-W2 model was configured for the Pangduo Reservoir to better understand the thermal structure and diurnal variation inside the reservoir under the local climate and hydrological conditions on the Qinghai-Tibetan Plateau. Observation data were used to verify the model, and the results showed that the average error of the 6 profile measured monthly from August to December 2016 was 0.1°C, and the root-mean-square error (RMSE) was 0.173°C. The water temperature from August 2016 to September 2017 was simulated by inputting measured data as model inputs. The results revealed that the reservoir of the Qinghai-Tibetan Plateau was a typical dimictic reservoir and the water mixed vertically at the end of March and the end of October. During the heating period, thermal stratification occurred, with strong diurnal variation in the epilimnion. The mean variance of the diurnal water temperature was 0.10 within a 5 m water depth but 0.04 in the whole water column. The mixing mode of inflow changed from undercurrent, horizontal-invaded flow and surface layer flow in one day. In winter, the diurnal variation was weak due to the thermal protection of the ice cover, while the mean variance of diurnal water temperature was 0.00 within both 5 m and the whole water column. Compared to reservoirs in areas with low altitude but the same latitude, significant differences occurred between the temperature structure of the low-altitude reservoir and the Pangduo Reservoir (P<0.01). The Pangduo Reservoir presented a shorter stratification period and weaker stratification stability, and the annual average SI value was 26.4 kg/m2, which was only 7.5% that of the low-altitude reservoir. The seasonal changes in the net heat flux received by the surface layers determined the seasonal cycle of stratification and mixing in reservoirs. This study provided a scientific understanding of the thermal changes in stratified reservoirs under the special geographical and meteorological conditions on the Qinghai-Tibetan Plateau. Moreover, this model can serve as a reference for adaptive management of similar dimictic reservoirs in cold and high-altitude areas.

Possible Effect of Climate Change on Surface-Water Photochemistry: A Model Assessment of the Impact of Browning on the Photodegradation of Pollutants in Lakes during Summer Stratification. Epilimnion vs. Whole-Lake Phototransformation

Water browning in lakes (progressive increase of the content of chromophoric dissolved organic matter, CDOM) has the potential to deeply alter the photodegradation kinetics of pollutants during summer stratification. Browning, which takes place as a consequence of climate change in several Nordic environments, causes the thermocline to be shallower, because higher CDOM decreases the penetration of sunlight inside the water column. Using a model approach, it is shown in this paper that pollutants occurring in the epilimnion would be affected differently depending on their main photodegradation pathway(s): almost no change for the direct photolysis, slight decrease in the degradation kinetics by the hydroxyl radicals (•OH, but the resulting degradation would be too slow for the process to be effective during summer stratification), considerable decrease for the carbonate radicals (CO3•−), increase for the excited triplet states of CDOM (3CDOM*) and singlet oxygen (1O2). Because it is difficult to find compounds that are highly reactive with CO3•− and poorly reactive with 3CDOM*, the degradation rate constant of many phenols and anilines would show a minimum with increasing dissolved organic carbon (DOC), because of the combination of decreasing CO3•− and increasing 3CDOM* photodegradation. In contrast, overall photodegradation would always be inhibited by browning when the whole water column (epilimnion + hypolimnion) is considered, either because of slower degradation kinetics in the whole water volume, or even at unchanged overall kinetics, because of unbalanced distribution of photoreactivity within the water column.

Aquatic Microplastic Research—A Critique and Suggestions for the Future

While there are numerous papers on microplastics (mps) being published every week, there is a need for improvement for the field to mature. The papers reporting numbers found in water bodies cannot be compared because there are no standard methods for collection and analysis. It is clear that using nets for sampling misses most of the microfibers, which are the most abundant form when whole water samples are analyzed, and that microscopic identification has a very high error rate compared to chemical analytical equipment which can also identify the polymers. It is clear that most animals studied eat mps; we should learn what attracts the animals to the mps and what proportion pass right through and are defecated vs those that move into the tissues. It is considered that mps are a vector for transfer of toxic chemicals into the food chain. Let us investigate to what degree what proportion of contaminants are removed in the digestive system vs. staying bound tightly to the mps. Experimental studies should also use environmentally relevant doses and the shapes and sizes of mps that are most abundant in the environment.

Methods for measuring and modelling plastic transport and accumulation in large rivers

&lt;p&gt;Although freshwater systems are known to be the transport paths of plastic debris to the ocean, studies in rivers are rare. In recent years, measurements are advancing, but they hardly address the spatial distribution of plastic debris in the whole water column. Waste collecting activities in the Nationalpark Donau-Auen &amp;#8211; a part of the Austrian Danube River to the East of Vienna &amp;#8211; indicate that increasing quantities of plastic waste can also be found near the banks and within the inundation areas of our rivers. The EU financed project &quot;PlasticFreeDanube&quot; tries to find the sources, environmental impacts, transported amounts and paths, compositions and possible plastic accumulation zones.&lt;/p&gt;&lt;p&gt;A robust, net-based device was developed which can be applied at high flow velocities and discharges even at large rivers. The device consists of a strong and stable equipment carrier allowing a steady positioning. Three frames can be equipped with 1-2 nets each, having different mesh sizes exposed over the whole water column. The methodology was tested in the Austrian Danube River, showing a high heterogeneity of microplastic concentrations over the cross-section but also vertically over the depth. It was found that even higher amounts of plastic can be transported in a subsurface layer or even bottom-near.&lt;/p&gt;&lt;p&gt;Three-dimensional numerical modelling has proven to be a great support in describing and analyzing plastic particle transport in flowing waters. Flow fields near river engineering structures such as groynes and guiding walls were characterized by the models as they are known to be plastic accumulation zones. The models can be used for predicting potential accumulation zones in Danubian inundation areas and can provide recommendations for creating &amp;#8220;artificial&amp;#8221; accumulation zones where plastic can be more easily extracted from the river.&lt;/p&gt;