Identification of novel candidate phosphatidic acid-binding proteins involved in the salt-stress response of Arabidopsis thaliana roots

2013 ◽  
Vol 450 (3) ◽  
pp. 573-581 ◽  
Author(s):  
Fionn McLoughlin ◽  
Steven A. Arisz ◽  
Henk L. Dekker ◽  
Gertjan Kramer ◽  
Chris G. de Koster ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Phosphatidic Acid ◽  
Stress Responses ◽  
Ribosomal Proteins ◽  
Binding Proteins ◽  
Affinity Purification ◽  
Lipid Binding ◽  
Biotic And Abiotic Stress ◽  
Salt Stress Response

PA (phosphatidic acid) is a lipid second messenger involved in an array of processes occurring during a plant's life cycle. These include development, metabolism, and both biotic and abiotic stress responses. PA levels increase in response to salt, but little is known about its function in the earliest responses to salt stress. In the present study we have combined an approach to isolate peripheral membrane proteins of Arabidopsis thaliana roots with lipid-affinity purification, to identify putative proteins that interact with PA and are recruited to the membrane in response to salt stress. Of the 42 putative PA-binding proteins identified by MS, a set of eight new candidate PA-binding proteins accumulated at the membrane fraction after 7 min of salt stress. Among these were CHC (clathrin heavy chain) isoforms, ANTH (AP180 N-terminal homology) domain clathrin-assembly proteins, a putative regulator of potassium transport, two ribosomal proteins, GAPDH (glyceraldehyde 3-phosphate dehydrogenase) and a PI (phosphatidylinositol) 4-kinase. PA binding and salt-induced membrane recruitment of GAPDH and CHC were confirmed by Western blot analysis of the cellular fractions. In conclusion, the approach of the present study is an effective way to isolate biologically relevant lipid-binding proteins and provides new leads in the study of PA-mediated salt-stress responses in roots.

Identification of novel candidate phosphatidic acid-binding proteins involved in the salt-stress response of Arabidopsis thaliana roots

2013 ◽  
Vol 451 (2) ◽  
pp. 343-343 ◽  
Author(s):  
F. Mcloughlin ◽  
S. A. Arisz ◽  
H. L. Dekker ◽  
G. Kramer ◽  
C. G. de Koster ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Response ◽  
Phosphatidic Acid ◽  
Binding Proteins ◽  
Salt Stress Response

scholarly journals CRK2 enhances salt tolerance in Arabidopsis thaliana by regulating endocytosis and callose deposition in connection with PLDα1

2018 ◽  
Author(s):  
Kerri Hunter ◽  
Sachie Kimura ◽  
Anne Rokka ◽  
Cuong Tran ◽  
Masatsugu Toyota ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Subcellular Localization ◽  
Stress Responses ◽  
Negative Regulator ◽  
Biotic And Abiotic Stress ◽  
Callose Deposition ◽  
Receptor Like Kinase ◽  
Stress Dependent

AbstractHigh salinity has become an increasingly prevalent source of stress to which plants need to adapt. The receptor-like protein kinases (RLKs), including the cysteine-rich receptor-like kinase (CRK) subfamily, are a highly expanded family of transmembrane proteins in plants and are largely responsible for communication between cells and the extracellular environment. Various CRKs have been implicated in biotic and abiotic stress responses, however their functions on a cellular level remain largely uncharacterized. Here we have shown that CRK2 enhances salt tolerance at the germination stage in Arabidopsis thaliana. We identified CRK2 as a negative regulator of endocytosis, under both normal growth conditions and salt stress. We also established that functional CRK2 is required for salt-induced callose deposition. In doing so, we revealed a novel role for callose deposition, in response to increased salinity, and demonstrated its importance for salt tolerance during germination. Using fluorescently tagged proteins we observed specific changes in CRK2’s subcellular localization in response to various stress treatments. Many of CRK2’s cellular functions were dependent on phospholipase D (PLD) activity, as were the subcellular localization changes. Thus we propose that CRK2 acts downstream of PLD during salt stress to regulate endocytosis and promote callose deposition, and that CRK2 adopts specific stress-dependent subcellular localization patterns in order to carry out its functions.One sentence summaryThe receptor-like kinase CRK2 acts in connection with PLDα1 to regulate endocytosis and callose deposition at plasmodesmata, enhancing salt tolerance in Arabidopsis thaliana.

scholarly journals Phosphatidic acid mediates salt stress response by regulation of MPK6 in Arabidopsis thaliana

2010 ◽  
Vol 188 (3) ◽  
pp. 762-773 ◽  
Author(s):  
Lijuan Yu ◽  
Jianing Nie ◽  
Chunyan Cao ◽  
Yakang Jin ◽  
Min Yan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Response ◽  
Phosphatidic Acid ◽  
Salt Stress Response

scholarly journals Interaction of SOS2 with Nucleoside Diphosphate Kinase 2 and Catalases Reveals a Point of Connection between Salt Stress and H2O2 Signaling in Arabidopsis thaliana

2007 ◽  
Vol 27 (22) ◽  
pp. 7771-7780 ◽  
Author(s):  
Paul E. Verslues ◽  
Giorgia Batelli ◽  
Stefania Grillo ◽  
Fernanda Agius ◽  
Yong-Sig Kim ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Stress Responses ◽  
Double Mutant ◽  
Nucleoside Diphosphate Kinase ◽  
Salt Resistance ◽  
Interacting Proteins ◽  
Salt Stress Response ◽  
Oxygen Signaling ◽  
Nucleoside Diphosphate Kinase 2

ABSTRACT SOS2, a class 3 sucrose-nonfermenting 1-related kinase, has emerged as an important mediator of salt stress response and stress signaling through its interactions with proteins involved in membrane transport and in regulation of stress responses. We have identified additional SOS2-interacting proteins that suggest a connection between SOS2 and reactive oxygen signaling. SOS2 was found to interact with the H2O2 signaling protein nucleoside diphosphate kinase 2 (NDPK2) and to inhibit its autophosphorylation activity. A sos2-2 ndpk2 double mutant was more salt sensitive than a sos2-2 single mutant, suggesting that NDPK2 and H2O2 are involved in salt resistance. However, the double mutant did not hyperaccumulate H2O2 in response to salt stress, suggesting that it is altered signaling rather than H2O2 toxicity alone that is responsible for the increased salt sensitivity of the sos2-2 ndpk2 double mutant. SOS2 was also found to interact with catalase 2 (CAT2) and CAT3, further connecting SOS2 to H2O2 metabolism and signaling. The interaction of SOS2 with both NDPK2 and CATs reveals a point of cross talk between salt stress response and other signaling factors including H2O2.

scholarly journals Identification of Salt Stress Responding Genes Using Transcriptome Analysis in Green Alga Chlamydomonas reinhardtii

2018 ◽  
Vol 19 (11) ◽  
pp. 3359 ◽  
Author(s):  
Ning Wang ◽  
Zhixin Qian ◽  
Manwei Luo ◽  
Shoujin Fan ◽  
Xuejie Zhang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Transcriptome Analysis ◽  
Stress Responses ◽  
Electron Flow ◽  
Saline Stress ◽  
Alternative Source ◽  
Salt Stress Response ◽  
Stress Responding

Salinity is one of the most important abiotic stresses threatening plant growth and agricultural productivity worldwide. In green alga Chlamydomonas reinhardtii, physiological evidence indicates that saline stress increases intracellular peroxide levels and inhibits photosynthetic-electron flow. However, understanding the genetic underpinnings of salt-responding traits in plantae remains a daunting challenge. In this study, the transcriptome analysis of short-term acclimation to salt stress (200 mM NaCl for 24 h) was performed in C. reinhardtii. A total of 10,635 unigenes were identified as being differently expressed by RNA-seq, including 5920 up- and 4715 down-regulated unigenes. A series of molecular cues were screened for salt stress response, including maintaining the lipid homeostasis by regulating phosphatidic acid, acetate being used as an alternative source of energy for solving impairment of photosynthesis, and enhancement of glycolysis metabolism to decrease the carbohydrate accumulation in cells. Our results may help understand the molecular and genetic underpinnings of salt stress responses in green alga C. reinhardtii.

scholarly journals Quantitative Proteome and PTMome Analysis of Arabidopsis Thaliana Root Responses to Persistent Osmotic and Salinity Stress.

2021 ◽  
Author(s):  
M C Rodriguez ◽  
D Mehta ◽  
M Tan ◽  
R G Uhrig
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Responses ◽  
Protein Level ◽  
Economic Losses ◽  
Plant Responses ◽  
Protein Abundance ◽  
Lysine Acetylation ◽  
Label Free ◽  
Proteome Level

ABSTRACT Abiotic stresses such as drought result in large annual economic losses around the world. As sessile organisms, plants cannot escape the environmental stresses they encounter, but instead must adapt to survive. Studies investigating plant responses to osmotic and/or salt stress have largely focused on short-term systemic responses, leaving our understanding of intermediate to longer-term adaptation (24 h - days) lacking. In addition to protein abundance and phosphorylation changes, evidence suggests reversible lysine acetylation may also be important for abiotic stress responses. Therefore, to characterize the protein-level effects of osmotic and salt stress, we undertook a label-free proteomic analysis of Arabidopsis thaliana roots exposed to 300 mM Mannitol and 150 mM NaCl for 24 h. We assessed protein phosphorylation, lysine acetylation and changes in protein abundance, detecting significant changes in 245, 35 and 107 total proteins, respectively. Comparison with available transcriptome data indicates that transcriptome- and proteome-level changes occur in parallel, while PTMs do not. Further, we find significant changes in PTMs and protein abundance involve different proteins from the same networks, indicating a multifaceted regulatory approach to prolonged osmotic and salt stress. In particular, we find extensive protein-level changes involving sulphur metabolism under both osmotic and salt conditions as well as changes in protein kinases and transcription factors that may represent new targets for drought stress signaling. Collectively, we find that protein-level changes continue to occur in plant roots 24 h from the onset of osmotic and salt stress and that these changes differ across multiple proteome levels.

scholarly journals Association of transcription factor WRKY56 gene from Populus simonii × P. nigra with salt tolerance in Arabidopsis thaliana

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7291 ◽  
Author(s):  
Lei Wang ◽  
Wenjing Yao ◽  
Yao Sun ◽  
Jiying Wang ◽  
Tingbo Jiang
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Germination Rate ◽  
Wrky Transcription Factor ◽  
Biotic And Abiotic Stress ◽  
Populus Simonii ◽  
Reading Frame

The WRKY transcription factor family is one of the largest groups of transcription factor in plants, playing important roles in growth, development, and biotic and abiotic stress responses. Many WRKY genes have been cloned from a variety of plant species and their functions have been analyzed. However, the studies on WRKY transcription factors in tree species under abiotic stress are still not well characterized. To understand the effects of the WRKY gene in response to abiotic stress, mRNA abundances of 102 WRKY genes in Populus simonii × P. nigra were identified by RNA sequencing under normal and salt stress conditions. The expression of 23 WRKY genes varied remarkably, in a tissue-specific manner, under salt stress. Since the WRKY56 was one of the genes significantly induced by NaCl treatment, its cDNA fragment containing an open reading frame from P. simonii × P. nigra was then cloned and transferred into Arabidopsis using the floral dip method. Under salt stress, the transgenic Arabidopsis over-expressed the WRKY56 gene, showing an increase in fresh weight, germination rate, proline content, and peroxidase and superoxide dismutase activity, when compared with the wild type. In contrast, transgenic Arabidopsis displayed a decrease in malondialdehyde content under salt stress. Overall, these results indicated that the WRKY56 gene played an important role in regulating salt tolerance in transgenic Arabidopsis.

scholarly journals Comparison between the Transcriptomes of ‘KDML105’ Rice and a Salt-Tolerant Chromosome Segment Substitution Line

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 742
Author(s):  
Nopphawitchayaphong Khrueasan ◽  
Panita Chutimanukul ◽  
Kitiporn Plaimas ◽  
Teerapong Buaboocha ◽  
Meechai Siangliw ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Pyruvate Dehydrogenase ◽  
Rice Genome ◽  
Pyruvate Dehydrogenase Kinase ◽  
Chromosome 1 ◽  
Salt Tolerant ◽  
Substitution Lines ◽  
Salt Stress Response

‘KDML105’ rice, known as jasmine rice, is grown in northeast Thailand. The soil there has high salinity, which leads to low productivity. Chromosome substitution lines (CSSLs) with the ‘KDML105’ rice genetic background were evaluated for salt tolerance. CSSL18 showed the highest salt tolerance among the four lines tested. Based on a comparison between the CSSL18 and ‘KDML105’ transcriptomes, more than 27,000 genes were mapped onto the rice genome. Gene ontology enrichment of the significantly differentially expressed genes (DEGs) revealed that different mechanisms were involved in the salt stress responses between these lines. Biological process and molecular function enrichment analysis of the DEGs from both lines revealed differences in the two-component signal transduction system, involving LOC_Os04g23890, which encodes phototropin 2 (PHOT2), and LOC_Os07g44330, which encodes pyruvate dehydrogenase kinase (PDK), the enzyme that inhibits pyruvate dehydrogenase in respiration. OsPHOT2 expression was maintained in CSSL18 under salt stress, whereas it was significantly decreased in ‘KDML105’, suggesting OsPHOT2 signaling may be involved in salt tolerance in CSSL18. PDK expression was induced only in ‘KDML105’. These results suggested respiration was more inhibited in ‘KDML105’ than in CSSL18, and this may contribute to the higher salt susceptibility of ‘KDML105’ rice. Moreover, the DEGs between ‘KDML105’ and CSSL18 revealed the enrichment in transcription factors and signaling proteins located on salt-tolerant quantitative trait loci (QTLs) on chromosome 1. Two of them, OsIRO2 and OsMSR2, showed the potential to be involved in salt stress response, especially, OsMSR2, whose orthologous genes in Arabidopsis had the potential role in photosynthesis adaptation under salt stress.

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