Long noncoding RNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429 in melanoma

Melanoma is the most malignant skin cancer, which account for most of skin-cancer-related deaths. Long noncoding RNA (lncRNA) is a class of noncoding RNAs with crucial roles in many cancers. However, the roles of lncRNAs in melanoma have not been well studied. In the present study, using public available data and clinical tissues samples, we found that lncRNA ILF3-AS1 is up-regulated in melanoma tissues and cell lines, and correlated with poor prognosis of melanoma patients. Functional experiments showed that knockdown of ILF3-AS1 inhibits melanoma cell proliferation, migration, and invasion. Mechanistically, we found that ILF3-AS1 interacts with EZH2, promotes the binding of EZH2 to the miR-200b/a/429 promoter, and represses miR-200b/a/429 expression. The expression of ILF3-AS1 is negatively correlated with that of miR-200b/a/429 in melanoma tissues. Moreover, inhibition of miR-200b/a/429 abrogates the biological roles of ILF3-AS1 knockdown on melanoma cell proliferation, migration, and invasion. In conclusion, these results demonstrate that melanoma-upregulated lncRNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429, and imply that ILF3-AS1 may be a potential prognostic biomarker and therapeutic target for melanoma.

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Long noncoding RNA HEIH promotes melanoma cell proliferation, migration and invasion via inhibition of miR-200b/a/429

Bioscience Reports ◽

10.1042/bsr20170682 ◽

2017 ◽

Vol 37 (3) ◽

Cited By ~ 29

Author(s):

Haiying Zhao ◽

Guoping Xing ◽

Yingying Wang ◽

Zengxiang Luo ◽

Guoyan Liu ◽

...

Keyword(s):

Cell Proliferation ◽

Melanoma Cell ◽

Noncoding Rna ◽

Prognostic Biomarker ◽

Ectopic Expression ◽

Migration And Invasion ◽

Transcriptional Inhibition ◽

Melanoma Cell Proliferation ◽

Melanoma Patients ◽

Poor Outcomes

Long noncoding RNAs (lncRNAs) are frequently dysregulated and have important roles in many diseases, particularly cancers. lncRNA-HEIH was first identified in hepatocellular carcinoma (HCC). The expression, clinical significance and roles of lncRNA-HEIH in melanoma are still unknown. In the present study, we found that lncRNA-HEIH is highly expressed in melanoma tissues and cell lines, associated with advanced clinical stages, and predicts poor outcomes in melanoma patients. Functional assays showed that ectopic expression of lncRNA-HEIH promotes melanoma cell proliferation, migration and invasion. Knockdown of lncRNA-HEIH inhibits melanoma cell proliferation, migration and invasion. Mechanistically, we revealed that lncRNA-HEIH directly binds to miR-200b/a/429 promoter and represses miR-200b/a/429 transcription. The expression of miR-200b is inversely associated with lncRNA-HEIH in melanoma tissues. Furthermore, overexpression of miR-200b/a/429 abrogates melanoma cell proliferation, migration and invasion enhanced by lncRNA-HEIH. In conclusion, we identified lncRNA-HEIH as a key oncogene in melanoma via transcriptional inhibition of miR-200b/a/429. Our data suggested that lncRNA-HEIH may serve as a promising prognostic biomarker and therapeutic target for melanoma.

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Abstract 2451: Long noncoding RNA OVAAL promotes melanoma cell proliferation through translational suppression of p27

10.1158/1538-7445.am2018-2451 ◽

2018 ◽

Author(s):

YUNA YUAN ZHANG ◽

Xu Guang Yan ◽

Margaret Farrelly ◽

Hamed Yari ◽

Yuchen Feng ◽

...

Keyword(s):

Cell Proliferation ◽

Melanoma Cell ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Melanoma Cell Proliferation ◽

Translational Suppression

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Up-regulation of long noncoding RNA FALEC predicts poor prognosis and promotes melanoma cell proliferation through epigenetically silencing p21

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2017.11.060 ◽

2017 ◽

Vol 96 ◽

pp. 1371-1379 ◽

Cited By ~ 17

Author(s):

Nana Ni ◽

Hao Song ◽

Xiaopo Wang ◽

Xiulian Xu ◽

Yiqun Jiang ◽

...

Keyword(s):

Cell Proliferation ◽

Melanoma Cell ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Poor Prognosis ◽

Melanoma Cell Proliferation

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Long Noncoding RNA CTD-2589M5.4 Inhibits Ovarian Cancer Cell Proliferation, Migration, and Invasion Via Downregulation of the Extracellular Matrix–Receptor Interaction Pathway

Cancer Biotherapy & Radiopharmaceuticals ◽

10.1089/cbr.2020.4429 ◽

2021 ◽

Author(s):

Yuanyuan Gu ◽

Ke Huang ◽

Min Zhang ◽

Fang Teng ◽

Lili Ge ◽

...

Keyword(s):

Ovarian Cancer ◽

Extracellular Matrix ◽

Cell Proliferation ◽

Cancer Cell ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Ovarian Cancer Cell ◽

Migration And Invasion ◽

Receptor Interaction ◽

Cancer Cell Proliferation

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Long Noncoding RNA MEG3 Suppresses Glioma Cell Proliferation, Migration, and Invasion by Acting as a Competing Endogenous RNA of miR-19a

Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics ◽

10.3727/096504017x14886689179993 ◽

2017 ◽

Vol 25 (9) ◽

pp. 1471-1478 ◽

Cited By ~ 60

Author(s):

Nan Qin ◽

Gui-Feng Tong ◽

Li-Wei Sun ◽

Xiao-Lin Xu

Keyword(s):

Cell Proliferation ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Glioma Cell ◽

Migration And Invasion ◽

Competing Endogenous Rna ◽

Glioma Cell Proliferation

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Long noncoding RNA MEG3 suppresses cell proliferation, migration and invasion, induces apoptosis and paclitaxel-resistance via miR-4513/PBLD axis in breast cancer cells

Cell Cycle ◽

10.1080/15384101.2020.1839700 ◽

2020 ◽

Vol 19 (23) ◽

pp. 3277-3288

Author(s):

Mingzhi Zhu ◽

Fang Wang ◽

Hailong Mi ◽

Lin Li ◽

Jing Wang ◽

...

Keyword(s):

Breast Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Breast Cancer Cells ◽

Migration And Invasion ◽

Paclitaxel Resistance

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Regulation of melanoma malignancy by the RP11-705C15.3/miR-145-5p/NRAS/MAPK signaling axis

Cancer Gene Therapy ◽

10.1038/s41417-020-00274-5 ◽

2020 ◽

Author(s):

Xiang-jun Chen ◽

Sha Liu ◽

Dong-mei Han ◽

De-zhi Han ◽

Wei-jing Sun ◽

...

Keyword(s):

Cell Proliferation ◽

Melanoma Cell ◽

Molecular Mechanisms ◽

Ectopic Expression ◽

Therapeutic Targets ◽

Mapk Signaling ◽

Migration And Invasion ◽

Functional Assays ◽

Melanoma Cell Proliferation ◽

Signaling Axis

AbstractMelanoma is a common lethal skin cancer. Dissecting molecular mechanisms driving the malignancy of melanoma may uncover potential therapeutic targets. We previously identified miR-145-5p as an important tumor-suppressive microRNA in melanoma. Here, we further investigated the roles of long non-coding RNAs (lncRNAs) in melanoma. We identified RP11-705C15.3, a regulator of miR-145-5p, as an oncogenic lncRNA in melanoma. RP11-705C15.3 competitively bound miR-145-5p, relieved the repressive roles of miR-145-5p on its target NRAS, upregulated NRAS expression, and activated MAPK signaling. In vitro functional assays revealed that ectopic expression of RP11-705C15.3 promoted melanoma cell proliferation, inhibited apoptosis, and promoted migration and invasion. Silencing of RP11-705C15.3 repressed melanoma cell proliferation, induced apoptosis, and repressed migration and invasion. Notably, the roles of RP11-705C15.3 in melanoma cell proliferation, apoptosis, migration and invasion are reversed by miR-145-5p overexpression. In vivo functional assays revealed that RP11-705C15.3 promoted melanoma tumor growth and metastasis, which were also reversed by miR-145-5p overexpression. Furthermore, we investigated the expression of RP11-705C15.3 in clinical melanoma tissues and found that RP11-705C15.3 was increased in melanoma tissues. High expression of RP11-705C15.3 was positively correlated with thickness, ulceration, metastasis, and inferior overall survival. Taken together, our findings suggest RP11-705C15.3 as a novel oncogene in melanoma, and highlight that the RP11-705C15.3/miR-145-5p/NRAS/MAPK signaling axis may be potential therapeutic targets for melanoma.

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