Long noncoding RNA MEG3 suppresses cell proliferation, migration and invasion, induces apoptosis and paclitaxel-resistance via miR-4513/PBLD axis in breast cancer cells

AbstractThe role of organic anion transporting polypeptide 1B3 (SLCO1B3) in breast cancer is still controversial. The clinical immunohistochemical results showed that a greater proportion of patients with negative lymph nodes, AJCC stage I, and histological grade 1 (P < 0.05) was positively correlated with stronger expression of SLCO1B3, and DFS and OS were also increased significantly in these patients (P = 0.041, P = 0.001). Further subgroup analysis showed that DFS and OS were significantly enhanced with the increased expression of SLCO1B3 in the ER positive subgroup. The cellular function assay showed that the ability of cell proliferation, migration and invasion was significantly enhanced after knockdown of SLCO1B3 expression in breast cancer cell lines. In contrast, the ability of cell proliferation, migration and invasion was significantly reduced after overexpress the SLCO1B3 in breast cancer cell lines (P < 0.05). Overexpression or knockdown of SLCO1B3 had no effect on the apoptotic ability of breast cancer cells. High level of SLCO1B3 expression can inhibit the proliferation, invasion and migration of breast cancer cells, leading to better prognosis of patients. The role of SLCO1B3 in breast cancer may be related to estrogen. SLCO1B3 will become a potential biomarker for breast cancer diagnosis and prognosis assessment.

Download Full-text

ILF3-AS1 promotes cell proliferation and inhibits cell apoptosis of breast cancer by binding with miR-4429 to upregulate RAB14

Human & Experimental Toxicology ◽

10.1177/0960327121989422 ◽

2021 ◽

pp. 096032712198942

Author(s):

Xiaoxue Zhang ◽

Xianxin Xie ◽

Kuiran Gao ◽

Xiaoming Wu ◽

Yanwei Chen ◽

...

Keyword(s):

Breast Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Breast Cancer Cell ◽

Cell Apoptosis ◽

Breast Cancer Cells ◽

Migration And Invasion ◽

Cancer Cell Proliferation ◽

Breast Cancer Cell Proliferation ◽

Cancer Tissues

As one of the leading causes of cancer-related deaths among women, breast cancer accounts for a 30% increase of incidence worldwide since 1970s. Recently, increasing studies have revealed that the long non-coding RNA ILF3-AS1 is involved in the progression of various cancers. Nevertheless, the role of ILF3-AS1 in breast cancer remains largely unknown. In the present study, we found that ILF3-AS1 was highly expressed in breast cancer tissues and cells. ILF3-AS1 silencing inhibited breast cancer cell proliferation, migration and invasion, and promoted cell apoptosis. ILF3-AS1 bound with miR-4429 in breast cancer cells. Moreover, RAB14 was a downstream target of miR-4429, and miR-4429 expression was negatively correlated with RAB14 or ILF3-AS1 expression in breast cancer tissues. The result of rescue experiments demonstrated that overexpression of RAB14 can reverse the inhibitory effect of ILF3-AS1 knockdown on breast cancer cell proliferation, migration and invasion. Overall, ILF3-AS1 promotes the malignant phenotypes of breast cancer cells by interacting with miR-4429 to regulate RAB14, which might offer a new insight into the underlying mechanism of breast cancer.

Download Full-text

Long noncoding RNA HOTAIR promotes invasion of breast cancer cells through chondroitin sulfotransferase CHST15

International Journal of Cancer ◽

10.1002/ijc.32319 ◽

2019 ◽

Vol 145 (9) ◽

pp. 2478-2487 ◽

Cited By ~ 14

Author(s):

Liang‐Chih Liu ◽

Yuan‐Liang Wang ◽

Pei‐Le Lin ◽

Xiang Zhang ◽

Wei‐Chung Cheng ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Breast Cancer Cells

Download Full-text

Long noncoding RNA H19 acts as a miR ‐340‐3p sponge to promote epithelial‐mesenchymal transition by regulating YWHAZ expression in paclitaxel‐resistant breast cancer cells

Environmental Toxicology ◽

10.1002/tox.22938 ◽

2020 ◽

Vol 35 (9) ◽

pp. 1015-1028 ◽

Cited By ~ 1

Author(s):

Lei Yan ◽

Shuo Yang ◽

Cheng‐Xu Yue ◽

Xin‐Yu Wei ◽

Wei Peng ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Breast Cancer Cells ◽

Epithelial Mesenchymal Transition ◽

Mesenchymal Transition

Download Full-text

Long noncoding RNA LINC02582 acts downstream of miR-200c to promote radioresistance through CHK1 in breast cancer cells

Cell Death and Disease ◽

10.1038/s41419-019-1996-0 ◽

2019 ◽

Vol 10 (10) ◽

Cited By ~ 11

Author(s):

Baiyao Wang ◽

Jieling Zheng ◽

Rong Li ◽

Yunhong Tian ◽

Jie Lin ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Breast Cancer Cells ◽

Molecular Mechanisms ◽

Inverse Correlation ◽

Deubiquitinating Enzyme ◽

Downstream Target ◽

Damage Response

Abstract Radiotherapy is essential to treat breast cancer and microRNA (miRNA) miR-200c is considered as a radiosensitizer of breast cancer. However, the molecular mechanisms by which miR-200c regulates radiosensitivity remain largely unknown. In the present study, we showed that induction of miR-200c led to widespread alteration in long noncoding RNA (lncRNA) expression in breast cancer cells. We identified lncRNA LINC02582 as a target of miR-200c. Inhibition of LINC02582 expression increased radiosensitvity, while overexpression of LINC02582 promoted radioresistance. Mechanistically, LINC02582 interacts with deubiquitinating enzyme ubiquitin specific peptidase 7 (USP7) to deubiquitinate and stabilize checkpoint kinase 1 (CHK1), a critical effector kinase in DNA damage response, thus promoting radioresistance. Furthermore, we detected an inverse correlation between the expression of miR-200c vs. LINC02582 and CHK1 in breast cancer samples. These findings identified LINC02582 as a downstream target of miR-200c linking miR-200c to CHK1, in which miR-200c increases radiosensitivity by downregulation of CHK1.

Download Full-text