scholarly journals Exploring the potential value of miR-148b-3p, miR-151b and miR-27b-3p as biomarkers in acute ischemic stroke

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Xiuli Cheng ◽  
Pengcheng Kan ◽  
Zhuolin Ma ◽  
Yaru Wang ◽  
Wen Song ◽  
...  

Cerebrovascular disease is the main cause of death in the world. Here, we explored whether circulating serum miR-148b-3p, miR-151b and miR-27b-3p could be as potential diagnostic biomarkers for diagnosing acute ischemic stroke. Seventy-seven IS patients and forty-two healthy controls matched for age and sex were enrolled in the present study. Blood samples were drawn from IS patients within the 24 h. The correlation analysis was performed by Spearman. The ability to distinguish patients from healthy controls was determined by receiver operating characteristic (ROC) curve. The expression of circulating serum miR-148b-3p was significantly decreased, whereas miR-151b and miR-27b-3p were elevated significantly compared with controls. ROC analysis showed area under the ROC curve (AUC) of miR-148b-3p, miR-151b and miR-27b-3p to be 0.6647, 0.6852 and 0.6657, respectively. While the AUC increased to 0.8103 for the combination of miR-148b-3p and miR-27b-3p. Blood miR-151b level was negatively correlated with insulin-like growth factor-1 (IGF-1), and miR-27b-3p level was negatively correlated with IGF-1 and insulin-like growth factor binding protein-3, respectively. Our findings suggest that miR-148b-3p, miR-151b and miR-27b-3p may serve as blood-based biomarkers for diagnosing ischemic stroke patients, and the combination of miR-148b-3p and miR-27b-3p may be more powerful.

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e99186 ◽  
Author(s):  
Jian-Hua Tang ◽  
Li-Li Ma ◽  
Tian-Xia Yu ◽  
Juan Zheng ◽  
Hui-Juan Zhang ◽  
...  

2021 ◽  
Author(s):  
Jie Hu ◽  
Birou Zhong ◽  
Yuqian Guo

Abstract Background: Neuropathic pain (NeP) characterized by neuroplasticity and neuroinflammatory change is a common complication associated with spinal metastasis. However, there are no reliable candidates for diagnosis and treatment. Recently, cancer research has incorporated molecules into the treatment of patients with NeP, therefore, it is necessary to find key molecules of NeP to provide new targets for diagnosis and treatment.Methods: We analyzed RNA-seq data around the expression of ENPP6 based on bioinformatic methods, including differentially expressed genes (DEGs), Gene Ontology (GO), protein-protein interaction (PPI) network, Kyoto Encyclopedia of Genes and Genomes (KEGG) and GSEA analyses, receiver operating characteristic (ROC) curve, immune cell infiltration and mutation analysis.Results: We divided with pain samples into the High and Low ENPP6 expression groups. A total of 231 DEGs were identified. GO and KEGG analysis showed that DEGs were mainly enriched in Inflammation and cancer associated pathways. GSEA analysis showed that DEGs was significantly enriched in ARF3 and P38/MK2, RHO and RAS, and BRAFT and AKT1/E17K pathway. Pearson’s correlation analysis showed that the expression of ENPP6 was significantly correlated with autophagy phenotype and immunophenotype. Immune infiltrating analysis showed that activated NK cells were significantly highly expressed in Low group. ROC analysis of ENPP6 suggested that the area under the ROC curve was 0.925. Mutation sites analysis showed that most of the mutations in ENPP4-7 were phosphorylation sites.Conclusion: This study provides novel insights into molecular mechanisms underlying NeP, and identifying ENPP6 may serve as potentially diagnostic biomarkers and/or therapeutic targets for NeP.


2021 ◽  
Vol 17 (2) ◽  
pp. 206
Author(s):  
Jeeun Lee ◽  
Jeongjae Lee ◽  
Minwoo Lee ◽  
Jae-Sung Lim ◽  
Jin Hyouk Kim ◽  
...  

Author(s):  
Hala Shaheen ◽  
Sayed Sobhy ◽  
Sherine El Mously ◽  
Manal Niazi ◽  
Mohammed Gomaa

2015 ◽  
Vol 6 (4) ◽  
pp. 264-275 ◽  
Author(s):  
Vasileios-Arsenios Lioutas ◽  
Freddy Alfaro-Martinez ◽  
Francisco Bedoya ◽  
Chen-Chih Chung ◽  
Daniela A. Pimentel ◽  
...  

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