Different Distributions of Kinetically Apparent Molecular Varieties of α2-Macroglobulin in Health and in Patients with Cryptogenic Fibrosing Alveolitis

1981 ◽  
Vol 60 (3) ◽  
pp. 261-265
Author(s):  
R. M. Topping ◽  
A. H. Craven ◽  
Susan Whiting ◽  
B. G. Rigden ◽  
M. Turner-Warwick ◽  
...  
Keyword(s):  
Protein Separation ◽  
Proteinase Inhibitors ◽  
Adult Population ◽  
Kinetic Method ◽  
Binding Modes ◽  
Fibrosing Alveolitis ◽  
Cryptogenic Fibrosing Alveolitis ◽  
Α2 Macroglobulin ◽  
Molecular Interpretation ◽  
Proteinase Inhibition

1. Proteinase inhibitors have been studied in whole serum by using a kinetic method that avoids potentially damaging protein separation procedures. 2. The α2-macroglobulin of an individual can be allocated unambiguously into one of seven categories according to the binding of trypsin to inhibitor in two kinetically apparent binding modes (β- and α-modes). 3. The distribution of α2-macroglobulin β:α ratios in a healthy adult population is defined, and shown to be independent of sex and age. 4. The distribution of β: α ratios in a group of patients with cryptogenic fibrosing alveolitis was found to be significantly different (P < 0.005) from the normal distribution. 5. Changes in the β: α ratio were noted in five of six patients with cryptogenic fibrosing alveolitis after treatment, but on no occasion when two healthy subjects were assessed a total of nine times. 6. The molecular interpretation and the possible importance of altered proteinase inhibition in inflammation and fibrosis are discussed.

Tenascin immunoreactivity in cryptogenic fibrosing alveolitis

1995 ◽  
Vol 175 (4) ◽  
pp. 415-420 ◽  
Author(s):  
W. A. H. Wallace ◽  
S. E. M. Howie ◽  
D. Lamb ◽  
D. M. Salter
Keyword(s):  
Fibrosing Alveolitis ◽  
Cryptogenic Fibrosing Alveolitis

scholarly journals Contents of plasmin, α1-antitrypsin and α2-macroglobulin in blood of patients with cerebral infarction and discircular encephalopathy before the treatment

2004 ◽  
Vol 3 (3) ◽  
pp. 86-89
Author(s):  
N. A. Kuznetsova ◽  
T. S. Chirikova ◽  
N. A. Krayushkina ◽  
V. N. Zorina
Keyword(s):  
Serum Concentration ◽  
Cerebral Infarction ◽  
Patient Group ◽  
Proteinase Inhibitors ◽  
Healthy People ◽  
Differential Diagnostics ◽  
Control Group ◽  
Serum Samples ◽  
Level Increase ◽  
Α2 Macroglobulin

The aim of this investigation was to define the concentrations of serum proteinase — plasmin — and some of proteinase inhibitors, such as α2-macroglobulin (MG) and α1-antitrypsin (AT), in order to study their role in cerebral infarction (CI) and discircular encephalopathy (DE) pathogenesis. We have investigated 30 patients with cerebral infarction, 30 patients with discircular encephalopathy and 60 serum samples of the healthy people as a control. Plasmin (Pl), AT and MG levels have been defined with rocket immunoelectrophoresis method. We have found a significant Pl decrease in both group patients as compared to the control. The decrease has been (95,83 ± 4,3) mkg/ml in CI patients’ serum, (83,49 ± 3,79) mkg/ml in DE patients and (112,98 ± 2,66) mkg/ml in the control group. AT concentration has been increased in DE patients’ serum [(2,44 ± 0,15) g/l], MG concentration has decreased significantly [(1,08 ± 0/09) g/l] as compared to the control. On the contrary, MG serum concentration (1,72 ± 0/08 g/l) in CI patient group has not differed from the control [(1,64 ± 0,04) g/l] but it has been accompanied by AT level increase [(2,50 ± 0,16) g/l at patients and (1,78 ± 0,04) g/l at control]. We think that these differences can be used during differential diagnostics of the investigated diseases.

scholarly journals Activity of kallikrein-kinin system at patients with asiderotic anemia

2004 ◽  
Vol 3 (2) ◽  
pp. 56-61
Author(s):  
N. N. Zhilkova
Keyword(s):  
Disease Duration ◽  
Proteinase Inhibitors ◽  
Stable State ◽  
Continuous Treatment ◽  
Kinin System ◽  
Α2 Macroglobulin ◽  
Short Disease Duration ◽  
Severity Of The Disease ◽  
Kallikrein Kinin System ◽  
Severity Degree

The aim of investigation is to study the state of kallikrein-kinin system (KKS) and proteinase inhibitors at patients with asiderotic anemia prior to and after the treatment. 58 patients with asiderotic anemia prior to the treatment and in dynamics after 3 weeks have been examined. KKS state has been estimated by kallikrein, prekallikrein, proteinase α1-inhibitor (Pα1I) and α2-macroglobulin (α2-MG) levels in blood plasma having been investigated by Paskhina’s et al.chromatographic method and by Nartikova’s and Paskhina’s unified enzymatic method.Patients with asiderotic anemia had the rise of KKS activity. Its change differences depending on the disease duration have been revealed. At short disease duration a regulated KKS activation and its stable state have been marked. After the treatment at patients with medium severity degree it happens a normalization of all KKS parameters, at patients with serious anemia the high activity of Pα1I persists. The continuous treatment of anemia has led to the development of pathological type activation that had persisted at patients after the treatment.It has been revealed that the degree of KKS activation intensity and its renewal after the implemented treatment depended on the duration and severity of the disease.

scholarly journals DIAGNOSTIC POSSIBILITIES OF MODERN BIOCHEMICAL STUDY OF SPUTUM FROM PATIENTS WITH CYSTIC FIBROSIS (LITERATURE REVIEW)

2019 ◽  
Vol 64 (1) ◽  
pp. 24-28 ◽  
Author(s):  
A. V. Kozlov ◽  
O. A. Gusyakova ◽  
A. A. Ereshchenko ◽  
A. V. Khaliulin
Keyword(s):  
Cystic Fibrosis ◽  
Molecular Mechanisms ◽  
Proteinase Inhibitors ◽  
Biochemical Study ◽  
Cost Effective ◽  
Laboratory Method ◽  
Non Invasive ◽  
Cost Effective Method ◽  
Α2 Macroglobulin ◽  
Biochemical Studies

The review presents the pathobiochemical and molecular mechanisms of sputum formation in patients with cystic fibrosis associated with the pathophysiological features of the disease. Statistical data on the prevalence of this pathology in the world and in the Russian Federation are presented. The mechanisms of sputum formation and disorders of the mucociliary apparatus, leading to the accumulation of viscous bronchopulmonary secret in cystic fibrosis, are considered. The principles of the relationship between the rheological properties of sputum and the formation of inflammation in the lungs with the addition of a concomitant specific microflora in the bronchopulmonary system in patients with cystic fibrosis are presented. Describes the opportunities for biochemical studies of sputum of patients with this pathology: determining the activity of enzymes (myeloperoxidase), the content of proteinase inhibitors (α2-macroglobulin and α1-antitrypsin) and proinflammatory cytokines (IL-8 and TNFa), concentrations of iron and ferriferous proteins (lactoferrin and ferritin), which makes biochemical studies of sputum available, non-invasive, quick and cost-effective method of diagnosis, which can be widely used as an auxiliary laboratory method and makes it possible to use these metabolites as diagnostic markers to assess the severity of inflammation and infection of the lower respiratory tract and predict the development of respiratory complications in patients with cystic fibrosis.

Exposure to Commonly Prescribed Drugs and the Etiology of Cryptogenic Fibrosing Alveolitis

1998 ◽  
Vol 157 (3) ◽  
pp. 743-747 ◽  
Author(s):  
RICHARD HUBBARD ◽  
ANDREA VENN ◽  
CHRIS SMITH ◽  
MARIE COOPER ◽  
IAN JOHNSTON ◽  
...  
Keyword(s):  
Fibrosing Alveolitis ◽  
Cryptogenic Fibrosing Alveolitis
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