Decreased expression of pigment epithelium-derived factor within the penile tissues contributes to erectile dysfunction in diabetic rats

2018 ◽  
Vol 132 (20) ◽  
pp. 2175-2188 ◽  
Author(s):  
Hongjie Qiao ◽  
Yuanyuan Zhang ◽  
Wenwen Lin ◽  
Yu-Feng Wang ◽  
Cristina M. Furdui ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Erectile Dysfunction ◽  
Pigment Epithelium ◽  
Diabetic Rats ◽  
No Synthase ◽  
Inflammatory Factor ◽  
Potential Implication ◽  
Pigment Epithelium Derived Factor ◽  
Tnf Α ◽  
Anti Inflammatory

Increased production of reactive oxygen species (ROS) and inflammation are major contributors to the development and progression of diabetes-associated erectile dysfunction (DMED). As an endogenous antioxidant and anti-inflammatory factor, the potential implication of pigment epithelium-derived factor (PEDF) in DMED has not been revealed. To assess the potential antioxidant and anti-inflammatory functions of PEDF in DMED, we first demonstrated that PEDF was significantly decreased at the levels of the mRNA and protein in the penis of diabetic rats compared with normal controls. To test the hypothesis that decreased the penile levels of PEDF are associated with oxidative stress and inflammation in DMED, an adenovirus expressing PEDF (Ad-PEDF) or the same titer of control virus (Ad-GFP) was intracavernously administered at 2 weeks after diabetic onset. After 6 weeks of treatment, we found that administration of Ad-PEDF could significantly increase erectile response to cavernosal nerve stimulation in the diabetic rats by restoring the endothelial NO synthase (eNOS), P-eNOS, and neuronal NO synthase (nNOS) protein levels to the standard levels represented in normal rats and by suppressing the levels of tumor necrosis factor-α (TNF-α) and oxidative stress. In conclusion, the present data indicated that the antioxidant and anti-inflammatory potential of PEDF plays important role in restoring erectile function by the inhibition of oxidative stress and TNF-α production.

Anti-inflammatory effects of pigment epithelium-derived factor in diabetic nephropathy

2008 ◽  
Vol 294 (5) ◽  
pp. F1166-F1173 ◽  
Author(s):  
Joshua J. Wang ◽  
Sarah X. Zhang ◽  
Robert Mott ◽  
Ying Chen ◽  
Ryan R. Knapp ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
High Glucose ◽  
Matrix Protein ◽  
Pigment Epithelium ◽  
Diabetic Rats ◽  
Renoprotective Effect ◽  
Pigment Epithelium Derived Factor ◽  
Control Virus ◽  
Anti Inflammatory ◽  
Proinflammatory Factors

Previously, we have reported that pigment epithelium-derived factor (PEDF) ameliorates albuminuria and inhibits matrix protein deposition in the kidney of streptozotocin (STZ)-induced diabetic rats, suggesting a renoprotective effect of PEDF in early stages of diabetic nephropathy. As inflammation is a major contributor to the development and progression of diabetic nephropathy, we examined in the present study whether PEDF inhibits renal inflammation in diabetic kidney. Diabetic rats received an intravenous injection of an adenovirus expressing PEDF (Ad-PEDF) or the same titer of a control virus. Three wk after the injection, diabetic rats treated with the control virus showed significantly elevated renal levels of proinflammatory factors such as ICAM-1, MCP-1, TNF-α, and VEGF compared with age-matched nondiabetic controls. Ad-PEDF effectively suppressed the overexpression of these proinflammatory factors in diabetic kidneys. In cultured primary human renal mesangial cells (HMC), the high-glucose medium-induced upregulation of VEGF and MCP-1 was largely blocked by PEDF. Furthermore, PEDF inhibited high glucose-induced activation of NF-κB, a key transcription factor mediating inflammatory responses, and hypoxia-inducible factor-1, a major activator of VEGF expression in HMC. These results suggest that the renoprotective effect of PEDF against diabetic nephropathy may be partially through its anti-inflammatory activity, likely by blocking the NF-κB and HIF-1 pathways.

AGGF1 is a novel anti-inflammatory factor associated with TNF-α-induced endothelial activation

2013 ◽  
Vol 25 (8) ◽  
pp. 1645-1653 ◽  
Author(s):  
Fang-Yuan Hu ◽  
Chong Wu ◽  
Yang Li ◽  
Ke Xu ◽  
Wen-Jing Wang ◽  
...  
Keyword(s):  
Endothelial Activation ◽  
Inflammatory Factor ◽  
Tnf Α ◽  
Anti Inflammatory

The Effect of Curcumin Nano-Suspension on Myocardial Fibrosis in Diabetic Rats

2020 ◽  
Vol 12 (10) ◽  
pp. 1215-1220
Author(s):  
Bingshuang Xue ◽  
Yi Xue ◽  
Jiaojiao Zhou ◽  
Qichao Yang
Keyword(s):  
Oxidative Stress ◽  
Myocardial Fibrosis ◽  
Hot Spot ◽  
Health And Safety ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Therapeutic Drugs ◽  
Tnf Α ◽  
Mda Content ◽  
And Oxidative Stress

The occurrence of complications of diabetic patients not only increases the difficulty and burden of treatment but also significantly affects the health and safety of patients. Traditional therapeutic drugs are prone to drug resistance, which affects the therapeutic effect. In recent years, the application of plant-derived natural compounds in the treatment of diseases has become a hot spot in the research of diabetes drugs. Curcumin has anti-tumor, anti-inflammation, anti-oxidation and antimicrobial effects, but the mechanism of its effect on cardiomyocytes in diabetic patients is not yet clear. In this study, curcumin was prepared into nano-preparations and its mechanism of action in the process of myocardial fibrosis in diabetic rats was further explored. We found that injection of curcumin nano-suspension can increase the LVIDd and LVFS of rats, while reducing the serum CKMB, LDH, AST and cTnI levels. Further exploration found that curcumin can reduce serum TNF- α and IL-1 β levels in diabetic rats, while increasing the SOD and GSH-Px activities of myocardial tissue, and reducing MDA content. These suggests that curcumin can reduce inflammation and oxidative stress in diabetic rats. Therefore, this study believes that curcumin nano-suspension can effectively inhibit diabetic cardiomyocyte fibrosis, oxidative stress, and inflammation and protect the rat myocardium.

scholarly journals Identification of Pigment Epithelium-Derived Factor as an Adipocyte-Derived Inflammatory Factor

2012 ◽  
Vol 18 (8) ◽  
pp. 1161-1168 ◽  
Author(s):  
Sangeeta S. Chavan ◽  
LaQueta K. Hudson ◽  
Jian Hua Li ◽  
Mahendar Ochani ◽  
Yael Harris ◽  
...  
Keyword(s):  
Pigment Epithelium ◽  
Inflammatory Factor ◽  
Pigment Epithelium Derived Factor

scholarly journals Antioxidant and Anti-Inflammatory Potential of Polyphenols Contained in Mediterranean Diet in Obesity: Molecular Mechanisms

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 985 ◽  
Author(s):  
Abdelhafid Nani ◽  
Babar Murtaza ◽  
Amira Sayed Khan ◽  
Naim Akhtar Khan ◽  
Aziz Hichami
Keyword(s):  
Oxidative Stress ◽  
Mediterranean Diet ◽  
Molecular Mechanisms ◽  
Nutrition Transition ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Metabolic Inflammation ◽  
Dietary Polyphenols ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
The Impact

Nutrition transition can be defined as shifts in food habits, and it is characterized by high-fat (chiefly saturated animal fat), hypercaloric and salty food consumption at the expense of dietary fibers, minerals and vitamins. Western dietary patterns serve as a model for studying the impact of nutrition transition on civilization diseases, such as obesity, which is commonly associated with oxidative stress and inflammation. In fact, reactive oxygen species (ROS) overproduction can be associated with nuclear factor-κB (NF-κB)-mediated inflammation in obesity. NF-κB regulates gene expression of several oxidant-responsive adipokines including tumor necrosis factor-α (TNF-α). Moreover, AMP-activated protein kinase (AMPK), which plays a pivotal role in energy homeostasis and in modulation of metabolic inflammation, can be downregulated by IκB kinase (IKK)-dependent TNF-α activation. On the other hand, adherence to a Mediterranean-style diet is highly encouraged because of its healthy dietary pattern, which includes antioxidant nutraceuticals such as polyphenols. Indeed, hydroxycinnamic derivatives, quercetin, resveratrol, oleuropein and hydroxytyrosol, which are well known for their antioxidant and anti-inflammatory activities, exert anti-obesity proprieties. In this review, we highlight the impact of the most common polyphenols from Mediterranean foods on molecular mechanisms that mediate obesity-related oxidative stress and inflammation. Hence, we discuss the effects of these polyphenols on a number of signaling pathways. We note that Mediterranean diet (MedDiet) dietary polyphenols can de-regulate nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) and NF-κB-mediated oxidative stress, and metabolic inflammation. MedDiet polyphenols are also effective in upregulating downstream effectors of several proteins, chiefly AMPK.

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