Virulence markers in Aeromonas hydrophila and Aeromonas veronii biovar sobria isolates from freshwater fish and from a diarrhoea case

2002 ◽  
Vol 93 (3) ◽  
pp. 414-419 ◽  
Author(s):  
C.J. Gonzalez-Serrano ◽  
J.A. Santos ◽  
M.L. Garcia-Lopez ◽  
A. Otero
Keyword(s):  
Freshwater Fish ◽  
Aeromonas Hydrophila ◽  
Aeromonas Veronii ◽  
Virulence Markers

scholarly journals Caracterización de la microbiota intestinal en robalo (Centropomus sp.) y aislamiento de bacterias probióticas potenciales

2020 ◽  
Vol 31 (3) ◽  
pp. e16036
Author(s):  
Adrian Edú Zatán Valdiviezo ◽  
Deysy Castillo Chunga ◽  
Arnaldo Edward Castañeda Vargas ◽  
Manuel Alberto Feria Zevallos ◽  
Odalis Epifanía Toledo Valdiviezo ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Vibrio Harveyi ◽  
Aeromonas Veronii

El objetivo del estudio fue caracterizar la microbiota del tracto intestinal de robalo (Centropomus sp), proveniente de medio natural y cautiverio, además de aislar e identificar bacterias con potencial probiótico. La microbiota intestinal se caracterizó mediante metagenómica dirigida al gen 16S ARNr. Además, se sembraron muestras de mucus intestinal en medio de cultivo MRS para el aislamiento bacteriano. La identificación molecular se hizo mediante la secuenciación del gen 16S ARNr. Las capacidades probióticas se evidenciaron mediante ensayos proteolíticos y de antagonismo bacteriano frente a Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii y Vibrio harveyi. Los géneros bacterianos con mayor abundancia fueron Clostridium, Photobacterium, Cetobacterium y Rubritepida. Las especies bacterianas, incluyendo Klebsiella sp, dos cepas de Weisiella cibaria y Lactococcus sp fueron aisladas en agar MRS a partir del tracto intestinal. Las cepas W. cibaria mostraron amplio espectro antagonista y actividad proteolítica positiva.

scholarly journals Aeromonas veronii Infection in Commercial Freshwater Fish: A Potential Threat to Public Health

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 608 ◽  
Author(s):  
Tong Li ◽  
Sayed Haidar Abbas Raza ◽  
Bintong Yang ◽  
Yufeng Sun ◽  
Guiqin Wang ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
16S Rdna ◽  
Virulence Genes ◽  
Serine Proteinase ◽  
Ctenopharyngodon Idella ◽  
Potential Threat ◽  
16S Rdna Sequence ◽  
Aeromonas Veronii ◽  
Culture Characteristics ◽  
Polymerase Chain

Aeromonas veronii is an important pathogen causing freshwater fish sepsis and ulcer syndrome. An increasing number of cases have demonstrated its significance as an aquatic zoonotic agent. The purpose of this study was to ensure the safety of freshwater products by evaluating the infection status of edible freshwater fish. In this experiment, we isolated A. veronii from several species of apparently healthy freshwater fish, including Carassius auratus, Cyprinus carpio, Ctenopharyngodon idella, and Silurus asotus. A. veronii was identified through bacterial staining, culture characteristics, and 16S rDNA gene sequence. In addition, polymerase chain reaction (PCR) was used to investigate the distribution of seven major virulence genes, including aerolysin (aer: 88.51%), cytotoxic enterotoxin (act: 71.26%), serine proteinase (ser: 54.02%), adhesin (Aha: 40.23%), phospholipase (lip: 45.98%), nuclease (exu: 51.72%), and quorum sensing-controlled virulence factor (LuxS: 59.77%). In total, 496 strains of Aeromonas were isolated, including 87 strains of A. veronii. The isolates of A. veronii were Gram-negative, rod-shaped bacteria, and the colonies are yellow on Rimler-Shotts (RS) medium and showed greater than 99% homology with A. veronii ATCC35624 according to analyses of the 16S rDNA sequence. Nearly 50% of the A. veronii isolates carried at least four or more virulence genes, 25% of the isolates carried at least five types of virulence genes, and 59.77% isolates carried the LuxS gene, and the isolates carrying more virulence genes were found to be more virulent. These results are of great significance for further improving the food safety assessment of freshwater aquatic products.

scholarly journals Genome Sequence of Aeromonas hydrophila ATCC 7966T: Jack of All Trades

2006 ◽  
Vol 188 (23) ◽  
pp. 8272-8282 ◽  
Author(s):  
Rekha Seshadri ◽  
Sam W. Joseph ◽  
Ashok K. Chopra ◽  
Jian Sha ◽  
Jonathan Shaw ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Environmental Protection Agency ◽  
Aeromonas Hydrophila ◽  
Resistance Mechanisms ◽  
Dissimilatory Sulfate Reduction ◽  
Virulence Markers ◽  
Virulence Potential ◽  
Lateral Flagellum ◽  
Wide Range ◽  
Polluted Waters

ABSTRACT The complete genome of Aeromonas hydrophila ATCC 7966T was sequenced. Aeromonas, a ubiquitous waterborne bacterium, has been placed by the Environmental Protection Agency on the Contaminant Candidate List because of its potential to cause human disease. The 4.7-Mb genome of this emerging pathogen shows a physiologically adroit organism with broad metabolic capabilities and considerable virulence potential. A large array of virulence genes, including some identified in clinical isolates of Aeromonas spp. or Vibrio spp., may confer upon this organism the ability to infect a wide range of hosts. However, two recognized virulence markers, a type III secretion system and a lateral flagellum, that are reported in other A. hydrophila strains are not identified in the sequenced isolate, ATCC 7966T. Given the ubiquity and free-living lifestyle of this organism, there is relatively little evidence of fluidity in terms of mobile elements in the genome of this particular strain. Notable aspects of the metabolic repertoire of A. hydrophila include dissimilatory sulfate reduction and resistance mechanisms (such as thiopurine reductase, arsenate reductase, and phosphonate degradation enzymes) against toxic compounds encountered in polluted waters. These enzymes may have bioremediative as well as industrial potential. Thus, the A. hydrophila genome sequence provides valuable insights into its ability to flourish in both aquatic and host environments.

scholarly journals The use of Cattapa Leaves Terminalia cattapa as Preventive and Curative Methods in Patin Catfish Pangasionodon hypophthalmus Infected With Aeromonas hydrophila

2008 ◽  
Vol 7 (1) ◽  
pp. 79 ◽  
Author(s):  
D. Wahjuningrum ◽  
N. Ashry ◽  
Sri Nuryati
Keyword(s):  
Freshwater Fish ◽  
Clinical Sign ◽  
Aeromonas Hydrophila ◽  
Susceptibility Test ◽  
Blood Picture ◽  
Antibacterial Test ◽  
Test Fish

<p>Motile Aeromonad Septicaemia (MAS) caused by <em>Aeromonas hydrophila</em> induced serious epidemics of ulcerative disease in freshwater fish including patin catfish <em>Pangasionodon hypophthalmus</em>. <em>In vitro</em> study for antibacterial test of cattapa leaves <em>Terminalia cattapa</em> (TC) were done previous to the <em>in vivo</em> test. The <em>in vitro</em> susceptibility test was performed at the dosages of 30, 60 and 90 g/l TC. At the <em>in vivo</em> test, fish were injected intramusculary with TC at the dosages of 60 g/l for the prevention and 120 g/l for curative efficacy. Results from blood picture, clinical sign and mortaliyt showed that TC were better and more effective as preventive than curative for MAS in patin catfish.</p> <p>Keywords :  <em>Terminalia cattapa</em>, <em>Aeromonas hydrophila</em>, patin catfish</p> <p> </p> <p>ABSTRAK</p> <p>Penyakit MAS (<em>Motile Aeromonad Septicaemia</em>) yang disebabkan oleh <em>Aeromonas hydrophila</em> sering menyebabkan wabah penyakit tukak pada ikan-ikan air tawar termasuk pada ikan patin <em>Pangasionodon hypophthalmus</em>. Uji antibakteri secara <em>in vitro</em> dari daun ketapang <em>Terminalia cattapa</em> (TC) dilakukan sebelum uji <em>in vivo</em>. Pada uji <em>in vitro</em> dilakukan pengujian aktivitas antibakteri TC terhadap <em>A. Hydrophila</em> pada dosis TC 30, 60 dan 90 g/l. Pada uji <em>in vivo</em>, ikan diinfeksi secara intramuskular dengan TC, untuk pencegahan dengan dosis 60 g/l TC dan pengobatan pada dosis 120 g/l TC. Hasil yang diperoleh dari gambaran darah, gejala klinis dan kematian ikan patin menunjukkan bahwa TC lebih baik dan sangat efektif untuk pencegahan daripada pengobatan untuk penyakit MAS pada ikan patin.</p> Kata kunci: <em>Terminalia cattapa</em>, <em>Aeromonas hydrophila</em>, ikan patin

scholarly journals Occurrence of parasitic and bacterial diseases in Thai freshwater fish

2020 ◽  
Vol 8 (10) ◽  
pp. 210-214
Author(s):  
Chutharat Kanchan ◽  
◽  
Puttachat Imjai ◽  
Nukoon Kanchan ◽  
Aonanong Chaiyara ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Aeromonas Hydrophila ◽  
Fish Stock ◽  
Parasitic Diseases ◽  
Fish Pathogens ◽  
Anabas Testudineus ◽  
Bacterial Diseases ◽  
Staphylococcus Epidermis ◽  
Channa Striata ◽  
Local Fisherman

The present study was aimed to investigate the occurrence of parasitic and bacterial diseases in some freshwater fish in NongBo reservoir, Maha Sarakham Province. The sampling was randomly collect from three sites by gillnets, bag nets and cast nets from the local fisherman during October 2015 to September 2016. A total of eight freshwater fish species were identified namely Oreochromis niloticus, Hampala dispar, Pristolepis fasciata, Trichopodus pectoralis, Anabas testudineus, Oxyeleotris marmorata, Channa striata and Clarias batrachus. The parasitic diseases found on the sampled fish specied were external parasites such as Trichodina sp., Dactylogyrus sp. and Digenea gen.sp. The bacterial diseases identified were Aeromonas hydrophila, Acinetobacter baumannii, Plesiomonas shigelloides, Serratia odorifera and Staphylococcus epidermis. The dominant species of bacterium was Aeromonas hydrophila. This study revealed that the natural fish stock could be risk from the fish pathogens both parasites and bacteria. Keywords: Reservoir, external parasite, fish pathogens.

Protein fingerprinting profiles in different strains of Aeromonas hydrophila isolated from diseased freshwater fish

2005 ◽  
Vol 21 (4) ◽  
pp. 587-591 ◽  
Author(s):  
Basanta Kumar Das ◽  
Surya Kanta Samal ◽  
Biswa Ranjan Samantaray ◽  
Prem Kumar Meher
Keyword(s):  
Freshwater Fish ◽  
Aeromonas Hydrophila ◽  
Protein Fingerprinting ◽  
Different Strains

scholarly journals Insight Into Whole Genome of Aeromonas veronii Isolated From Freshwater Fish by Resistome Analysis Reveal Extensively Antibiotic Resistant Traits

2021 ◽  
Vol 12 ◽  
Author(s):  
Rungnapa Sakulworakan ◽  
Putita Chokmangmeepisarn ◽  
Nguyen Dinh-Hung ◽  
Elayaraja Sivaramasamy ◽  
Ikuo Hirono ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Whole Genome Sequencing ◽  
Freshwater Fish ◽  
Genome Sequencing ◽  
Whole Genome ◽  
Antibiotic Resistant ◽  
Aeromonas Veronii ◽  
Insight Into

Aeromonas veronii outbreaks in tilapia farming caused relatively high mortalities, and the bacteria was resistant to many kinds of antimicrobials used in Thailand aquaculture. According to the CLSI standard, the determination of antimicrobials efficacy has been limited to phenotypic analyses, and a genomics study is required. This research aimed to analyze the resistome of A. veronii isolated from diseased tilapia in Chainat, Nong Khai, and Uttaradit provinces in Thailand. A total of 12 isolates of A. veronii were identified based on the gyrB sequencing and then, the MIC values to eight antimicrobials (AMP, AML, GEN, ENR, OXO, OTC, SXT, and FFC) were determined. According to the MIC patterns, whole genome sequencing (WGS) of five representatives and resistome analysis were performed, including 15 genomes of A. veronii isolated from freshwater fish available in the NCBI. All tilapia isolates were susceptible to FFC but resistant to AML and AMP while OTC resistance was the most dominant. In addition to the WGS analysis, 4.5 Mbp of A. veronii was characterized. A total of 20 ARGs were detected by resistome analysis and 16 genes were shared among the A. veronii population. In conclusion, A. veronii strains isolated from tilapia exhibited a resistance to several antimicrobials and multidrug resistance (MDR) which was related to the presence of multiple ARGs. Aeromonas veronii shared the ARGs in their population worldwide with a possibility of a plasmid-mediated acquisition due to the presence of resistance islands.

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