scholarly journals Evaluation of antimicrobial activity of synthesised silver nanoparticles using Thymus kotschyanus aqueous extract

2018 ◽  
Vol 12 (8) ◽  
pp. 1114-1117 ◽  
Author(s):  
Maryam Gholami ◽  
Kiana Shahzamani ◽  
Abdolrazagh Marzban ◽  
Hamed Esmaeil Lashgarian
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Javier Mussin ◽  
Viviana Robles-Botero ◽  
Rocío Casañas-Pimentel ◽  
Florencia Rojas ◽  
Letizia Angiolella ◽  
...  

AbstractCombining traditional medicine with nanotechnology therefore opens the door to innovative strategies for treating skin and soft tissue infections (SSTIs) and also contributes to the fight against the rise of antimicrobial resistance. Acanthospermum australe (Loefl.) Kuntze is a medicinal plant used by indigenous peoples in northeastern Argentina to treat SSTIs. Spherical and stable silver nanoparticles (AgNPs) of 14 ± 2 nm were synthesized from the aqueous extract of A. australe and silver nitrate. The antimicrobial activity against main species causing SSTIs and cytotoxicity on peripheral blood mononuclear cells of AgNP solution and its synthesis components were evaluated. Compared to its synthesis components, AgNP solution showed greater antimicrobial activity and lower cytotoxicity. The antimicrobial activity of AgNPs was due to the silver and not to the metabolites of the aqueous extract present on the surface of the nanoparticles. The plant extract played an important role in the formation of stable AgNPs and acted as a modulator of cytotoxic and immune responses.


INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (08) ◽  
pp. 25-29
Author(s):  
Jambuwant A. Kadam ◽  
Mahesh A Karale ◽  
Pushpa Karale

The present work deals with the green synthesis of silver nanoparticle from aqueous extract of Pergularia daemia as reducing agent and evaluation of the antimicrobial potential of synthesized green nanoparticles (GNPs). The synthesized silver nanoparticles (SNPs) were characterized by UltravioletVisible absorption spectroscopy (UV-Vis) and high-resonance transmission electron microscopy (TEM) analysis. Visual observation showed that the color of the fresh leaf extracts of P. daemia turned into dark brown after incubation of 24 h with Ag precursors. The TEM analysis showed that nanoparticles were spherical in shape and the size was found to be in the range of 7-22 nm. The green synthesized nanoparticles showed concentration dependent (25 µg/mL, 50 µg/mL and 100 µg/mL) noteworthy antimicrobial activity against E. coli, S. aureus and B. subtilis with ciprofloxacin as a standard. Research findings conclude that GNPs possess superior antimicrobial potential and it is a new option to combat antibiotic resistance.


2019 ◽  
Vol 27 (11) ◽  
pp. 1155-1160
Author(s):  
Narayanasamy Tamil Selvi ◽  
Rangaswamy Navamathavan ◽  
Hak Yong Kim ◽  
Rajkumar Nirmala

Author(s):  
HEMADEVI N ◽  
VENKATALAKSHMI P

Objective: The proposed study is carried out to synthesize and characterize silver nanoparticles (AgNPs) using rhizomes of Asparagus racemosus and to evaluate its antimicrobial activity in vitro. Methods: Aqueous extract of A. racemosus rhizomes was prepared and subjected to preliminary phytochemical screening. AgNPs of A. racemosus rhizomes were prepared using standard procedure. The synthesized particles were characterized using ultraviolet-visible spectrophotometry, Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopic (SEM) analysis. Further, these nanoparticles were subjected to in vitro antimicrobial studies. Results: Preliminary phytochemical analysis showed the presence of tannins, phlobatannins, saponins, flavonoids, terpenoids, triterpenoids, alkaloids, carbohydrates, proteins, anthraquinones, polyphenols, and glycosides. The study of synthesized AgNPs showed a sharp absorbance at 410 nm. The results of FTIR analysis confirmed the presence of phenol, alkanes, aliphatic amine, secondary alcohol, alkenes, and aromatic amines. SEM analysis of the AgNPs revealed higher density polydispersed AgNPs of various sizes. The AgNPs synthesized from rhizome extract showed higher toxicity toward tested microorganisms when compared to extract alone. Conclusion: It has been concluded that silver nanoparticles of A. racemosus could be a good source of plant-derived antimicrobials.


Author(s):  
M. Linga Rao ◽  
Bhumi G ◽  
Savithramma N

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent.  The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals.  In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica.  The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM).  The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size.  These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species.  These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.


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