scholarly journals Antimicrobial and cytotoxic activity of green synthesis silver nanoparticles targeting skin and soft tissue infectious agents

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Javier Mussin ◽  
Viviana Robles-Botero ◽  
Rocío Casañas-Pimentel ◽  
Florencia Rojas ◽  
Letizia Angiolella ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Soft Tissue ◽  
Indigenous Peoples ◽  
Aqueous Extract ◽  
Mononuclear Cells ◽  
Infectious Agents ◽  
Peripheral Blood Mononuclear ◽  
Innovative Strategies ◽  
Northeastern Argentina

AbstractCombining traditional medicine with nanotechnology therefore opens the door to innovative strategies for treating skin and soft tissue infections (SSTIs) and also contributes to the fight against the rise of antimicrobial resistance. Acanthospermum australe (Loefl.) Kuntze is a medicinal plant used by indigenous peoples in northeastern Argentina to treat SSTIs. Spherical and stable silver nanoparticles (AgNPs) of 14 ± 2 nm were synthesized from the aqueous extract of A. australe and silver nitrate. The antimicrobial activity against main species causing SSTIs and cytotoxicity on peripheral blood mononuclear cells of AgNP solution and its synthesis components were evaluated. Compared to its synthesis components, AgNP solution showed greater antimicrobial activity and lower cytotoxicity. The antimicrobial activity of AgNPs was due to the silver and not to the metabolites of the aqueous extract present on the surface of the nanoparticles. The plant extract played an important role in the formation of stable AgNPs and acted as a modulator of cytotoxic and immune responses.

scholarly journals Evaluation of antimicrobial activity of synthesised silver nanoparticles using Thymus kotschyanus aqueous extract

2018 ◽  
Vol 12 (8) ◽  
pp. 1114-1117 ◽  
Author(s):  
Maryam Gholami ◽  
Kiana Shahzamani ◽  
Abdolrazagh Marzban ◽  
Hamed Esmaeil Lashgarian
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Thymus Kotschyanus

scholarly journals In VitroandIn VivoCytogenotoxic Effects of Hot Aqueous Extract ofAchyrocline satureioides(Lam.) DC.

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
L. N. Cariddi ◽  
M. C. Sabini ◽  
F. M. Escobar ◽  
R. Bacchetti ◽  
I. Montironi ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Mononuclear Cells ◽  
Micronucleus Test ◽  
Cell Damage ◽  
Human Peripheral Blood ◽  
Chemical Study ◽  
Blue Dye ◽  
Peripheral Blood Mononuclear ◽  
Toxicological Studies

In this work we extend the toxicological studies of hot aqueous extract ofA. satureioides(As-HAE) evaluating cytotoxic and apoptotic effects on human peripheral blood mononuclear cells (PBMCs). We also determine genotoxic action of this extractin vivo. In addition, the extract was chemically characterized. Finally, we established a comparison with previous data of cold aqueous extract. The As-HAE induced cytotoxicity on PBMCs determined by trypan blue dye exclusion (CC50= 653 μg/mL) and MTT (CC50= 588 μg/mL) assays being more toxic than cold extract. However, As-HAE as well as cold extract did not induce apoptosis measured by Hoechst 33258 staining, TUNEL assay, and DNA fragmentation analysis. Thein vivomicronucleus test showed that As-HAE exerted cytogenotoxic effects on bone marrow of mice, contrary to what was observed with cold extract. The chemical study of As-HAE allowed identifying the flavonoids found in cold extract: luteolin, quercetin, and 3-O-methylquercetin, but at higher concentrations. We suggest that toxic effects induced by As-HAE could be due to high concentrations of these flavonoids. Given that As-HAE is the most used in folkloric medicine, its administration should be controlled in order to prevent potential cell damage.

scholarly journals Bactericidal and Cytotoxic Properties of Silver Nanoparticles

2019 ◽  
Vol 20 (2) ◽  
pp. 449 ◽  
Author(s):  
Chengzhu Liao ◽  
Yuchao Li ◽  
Sie Tjong
Keyword(s):  
Silver Nanoparticles ◽  
Mammalian Cells ◽  
Food Packaging ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Umbilical Vein ◽  
Drug Carriers ◽  
Wound Dressings ◽  
Circulation System ◽  
Peripheral Blood Mononuclear

Silver nanoparticles (AgNPs) can be synthesized from a variety of techniques including physical, chemical and biological routes. They have been widely used as nanomaterials for manufacturing cosmetic and healthcare products, antimicrobial textiles, wound dressings, antitumor drug carriers, etc. due to their excellent antimicrobial properties. Accordingly, AgNPs have gained access into our daily life, and the inevitable human exposure to these nanoparticles has raised concerns about their potential hazards to the environment, health, and safety in recent years. From in vitro cell cultivation tests, AgNPs have been reported to be toxic to several human cell lines including human bronchial epithelial cells, human umbilical vein endothelial cells, red blood cells, human peripheral blood mononuclear cells, immortal human keratinocytes, liver cells, etc. AgNPs induce a dose-, size- and time-dependent cytotoxicity, particularly for those with sizes ≤10 nm. Furthermore, AgNPs can cross the brain blood barrier of mice through the circulation system on the basis of in vivo animal tests. AgNPs tend to accumulate in mice organs such as liver, spleen, kidney and brain following intravenous, intraperitoneal, and intratracheal routes of administration. In this respect, AgNPs are considered a double-edged sword that can eliminate microorganisms but induce cytotoxicity in mammalian cells. This article provides a state-of-the-art review on the synthesis of AgNPs, and their applications in antimicrobial textile fabrics, food packaging films, and wound dressings. Particular attention is paid to the bactericidal activity and cytotoxic effect in mammalian cells.

scholarly journals Endogenous feline leukemia virus siRNA transcription may interfere with exogenous FeLV infection

2021 ◽  
Author(s):  
Elliott S. Chiu ◽  
Sue VandeWoude
Keyword(s):  
Mononuclear Cells ◽  
Cell Function ◽  
Leukemia Virus ◽  
Endogenous Retroviruses ◽  
Domestic Cat ◽  
Feline Leukemia Virus ◽  
Infectious Agents ◽  
Biological Functions ◽  
Peripheral Blood Mononuclear ◽  
Solo Ltrs

AbstractEndogenous retroviruses (ERVs) are increasingly recognized for biological impacts on host cell function and susceptibility to infectious agents, particularly in relation to interactions with exogenous retroviral progenitors (XRVs). ERVs can simultaneously promote and restrict XRV infections using different mechanisms that are virus- and host-specific. The majority of endogenous-exogenous retroviral interactions have been evaluated in experimental mouse or chicken systems which are limited in their ability to extend findings to naturally infected outbred animals. Feline leukemia virus (FeLV) has a relatively well-characterized endogenous retrovirus with a coexisting virulent exogenous counterpart and is endemic worldwide in domestic cats. We have previously documented an association between endogenous FeLV LTR copy number and abrogated exogenous FeLV in naturally infected cats and experimental infections in tissue culture. Analyses described here examine limited FeLV replication in experimentally infected peripheral blood mononuclear cells. We further examine NCBI Sequence Read Archive RNA transcripts to evaluate enFeLV transcripts and RNA interference precursors. We find that lymphoid-derived tissues, which are experimentally less permissive to exogenous FeLV infection, transcribe higher levels of enFeLV under basal conditions. Transcription of enFeLV-LTR segments is significantly greater than other enFeLV genes. We documented transcription of a 21-nt miRNA just 3′ to the enFeLV 5′-LTR in the feline miRNAome of all datasets evaluated (n=27). Our findings point to important biological functions of enFeLV transcription linked to solo LTRs distributed within the domestic cat genome, with potential impacts on domestic cat exogenous FeLV susceptibility and pathogenesis.ImportanceEndogenous retroviruses (ERVs) are increasingly implicated in host cellular processes and susceptibility to infectious agents, specifically regarding interactions with exogenous retroviral progenitors (XRVs). Exogenous feline leukemia virus (FeLV) and its endogenous counterpart (enFeLV) represent a well characterized, naturally occurring XRV-ERV dyad. We have previously documented an abrogated FeLV infection in both naturally infected cats and experimental fibroblast infections that harbor higher enFeLV proviral loads. Using an in silico approach, we provide evidence of miRNA-transcription that are produced in tissues most important for FeLV infection, replication, and transmission. Our findings point to important biological functions of enFeLV transcription linked to solo-LTRs distributed within the feline genome, with potential impacts on domestic cat exogenous FeLV susceptibility and pathogenesis. This body of work provides additional evidence of RNAi as a mechanism of viral interference and is a demonstration of ERV exaptation by the host to defend against related XRVs.

SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF PERGULARIA DAEMIA AND ANALYSIS OF ANTIMICROBIAL ACTIVITY

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (08) ◽  
pp. 25-29
Author(s):  
Jambuwant A. Kadam ◽  
Mahesh A Karale ◽  
Pushpa Karale
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Visual Observation ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
E Coli ◽  
Antimicrobial Potential ◽  
Transmission Electron ◽  
Research Findings

The present work deals with the green synthesis of silver nanoparticle from aqueous extract of Pergularia daemia as reducing agent and evaluation of the antimicrobial potential of synthesized green nanoparticles (GNPs). The synthesized silver nanoparticles (SNPs) were characterized by UltravioletVisible absorption spectroscopy (UV-Vis) and high-resonance transmission electron microscopy (TEM) analysis. Visual observation showed that the color of the fresh leaf extracts of P. daemia turned into dark brown after incubation of 24 h with Ag precursors. The TEM analysis showed that nanoparticles were spherical in shape and the size was found to be in the range of 7-22 nm. The green synthesized nanoparticles showed concentration dependent (25 µg/mL, 50 µg/mL and 100 µg/mL) noteworthy antimicrobial activity against E. coli, S. aureus and B. subtilis with ciprofloxacin as a standard. Research findings conclude that GNPs possess superior antimicrobial potential and it is a new option to combat antibiotic resistance.

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