Single-lens mass measurement in the high-magnification microlensing event Gaia19bld located in the Galactic disc

In this paper, we review the astrophysical application of gravitational microlensing. After introducing the history of gravitational lensing, we present the key equations and concept of microlensing. The most frequent microlensing events are single-lens events and historically it has been used for searching dark matter in the form of compact astrophysical halo objects in the Galactic halo. We discuss about the degeneracy problem in the parameters of lens and perturbation effects that can partially break the degeneracy between the lens parameters. The rest of paper is about the astrophysical applications of microlensing. One of the important applications is in the stellar physics by probing the surface of source stars in the high magnification microlensing events. The astrometric and polarimetric observations will be complimentary for probing the atmosphere and stellar spots on the surface of source stars. Finally we discuss about the future projects as space-based telescopes for parallax and astrometry observations of microlensing events. With this project, we would expect to produce a complete stellar and remnant mass function and study the structure of Galaxy in term of distribution of stars along our line of sight towards the center of galaxy.

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The Microstructure of Shock-Synthesized Diamond

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006163x ◽

1967 ◽

Vol 25 ◽

pp. 324-325

Author(s):

Lucien F. Trueb

Keyword(s):

Single Crystals ◽

Preferred Orientation ◽

High Magnification ◽

Texture Pattern ◽

Shock Process ◽

New Type ◽

Diffraction Diagram

A new type of synthetic industrial diamond formed by an explosive shock process has been recently developed by the Du Pont Company. This material consists of a mixture of two basically different forms, as shown in Figure 1: relatively flat and compact aggregates of acicular crystallites, and single crystals in the form of irregular polyhedra with straight edges.Figure 2 is a high magnification micrograph typical for the fibrous aggregates; it shows that they are composed of bundles of crystallites 0.05-0.3 μ long and 0.02 μ. wide. The selected area diffraction diagram (insert in Figure 2) consists of a weak polycrystalline ring pattern and a strong texture pattern with arc reflections. The latter results from crystals having preferred orientation, which shows that in a given particle most fibrils have a similar orientation.

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Macromolecular structures of biological specimens are not obscured by controlled osmium impregnation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100076639 ◽

1983 ◽

Vol 41 ◽

pp. 606-607

Author(s):

Klaus-Ruediger Peters ◽

Samuel A. Green

Keyword(s):

Thin Films ◽

Electrical Conductivity ◽

Critical Point ◽

Metal Films ◽

High Magnification ◽

Osmium Impregnation ◽

Instrumental Resolution ◽

20 Nm ◽

Continuous Metal

High magnification imaging of macromolecules on metal coated biological specimens is limited only by wet preparation procedures since recently obtained instrumental resolution allows visualization of topographic structures as smal l as 1-2 nm. Details of such dimensions may be visualized if continuous metal films with a thickness of 2 nm or less are applied. Such thin films give sufficient contrast in TEM as well as in SEM (SE-I image mode). The requisite increase in electrical conductivity for SEM of biological specimens is achieved through the use of ligand mediated wet osmiuum impregnation of the specimen before critical point (CP) drying. A commonly used ligand is thiocarbohvdrazide (TCH), first introduced to TEM for en block staining of lipids and glvcomacromolecules with osmium black. Now TCH is also used for SEM. However, after ligand mediated osinification nonspecific osmium black precipitates were often found obscuring surface details with large diffuse aggregates or with dense particular deposits, 2-20 nm in size. Thus, only low magnification work was considered possible after TCH appl ication.

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Conformation of eukaryotic ribosomal RNAs

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100076561 ◽

1983 ◽

Vol 41 ◽

pp. 592-593

Author(s):

M. Boublik ◽

G. Thornton ◽

G. Oostergetel ◽

J.F. Hainfeld ◽

J.S. Wall

Keyword(s):

Molecular Weight ◽

Mass Measurement ◽

Carbon Film ◽

Structural Complexity ◽

Scanning Transmission Electron Microscope ◽

Electron Microscopic ◽

Pure Nitrogen ◽

Freeze Dried ◽

Scanning Transmission ◽

Partially Unfolded

Understanding the structural complexity of ribosomes and their role in protein synthesis requires knowledge of the conformation of their components - rRNAs and proteins. Application of dedicated scanning transmission electron microscope (STEM), electrical discharge of the support carbon film in an atmosphere of pure nitrogen, and determination of the molecular weight of individual rRNAs enabled us to obtain high resolution electron microscopic images of unstained freeze-dried rRNA molecules from BHK cells in a form suitable for evaluation of their 3-D structure. Preliminary values for the molecular weight of 28S RNA from the large and 18S RNA from the small ribosomal subunits as obtained by mass measurement were 1.84 x 106 and 0.97 x 106, respectively. Conformation of rRNAs consists, in general, of alternating segments of intramolecular hairpin stems and single stranded loops in a proportion which depends on their ionic environment, the Mg++ concentration in particular. Molecules of 28S RNA (Fig. 1) and 18S RNA (not shown) obtained by freeze-drying from a solution of 60 mM NH+4 acetate and 2 mM Mg++ acetate, pH 7, appear as partially unfolded coils with compact cores suggesting a high degree of ordered secondary structure.

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SEM Examination of a Used Diamond Knife

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066310 ◽

1971 ◽

Vol 29 ◽

pp. 452-453

Author(s):

J. Temple Black

Keyword(s):

Electron Microscope ◽

High Voltage ◽

High Magnification ◽

Metallic Materials ◽

Wide Spread ◽

Thin Sections ◽

Transmission Electron ◽

Scanning Transmission ◽

Scanning Electron ◽

Diamond Knife

Since its introduction by Fernandez-Moran, the diamond knife has gained wide spread usage as a common material for cutting of thin sections of biological and metallic materials into thin films for examination in the transmission electron microscope. With the development of high voltage E.M. and scanning transmission E.M., microtomy applications will become increasingly important in the preparation of specimens. For those who can afford it, the diamond knife will thus continue to be an important tool to accomplish this effort until a cheaper but equally strong and sharp tool is found to replace the diamond, glass not withstanding.In Figs. 1 thru 3, a first attempt was made to examine the edge of a used (β=45°) diamond knife by means of the scanning electron microscope. Because diamond is conductive, first examination was tried without any coating of the diamond. However, the contamination at the edge caused severe charging during imaging. Next, a thin layer of carbon was deposited but charging was still extensive at high magnification - high voltage settings. Finally, the knife was given a light coating of gold-palladium which eliminated the charging and allowed high magnification micrographs to be made with reasonable resolution.

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Analysis of the Anterior Secretory Cells of Onchidohs Muricata (Nudibranchia)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099490 ◽

1981 ◽

Vol 39 ◽

pp. 614-615

Author(s):

Roy Skidmore

Keyword(s):

Endoplasmic Reticulum ◽

Secretory Granules ◽

Golgi Body ◽

The Body ◽

Secretory Cells ◽

Secretory Vesicles ◽

High Magnification ◽

Large Numbers ◽

Membrane Bound ◽

Sole Of The Foot

The long-necked secretory cells in Onchidoris muricata are distributed in the anterior sole of the foot. These cells are interspersed among ciliated columnar and conical cells as well as short-necked secretory gland cells. The long-necked cells contribute a significant amount of mucoid materials to the slime on which the nudibranch travels. The body of these cells is found in the subepidermal tissues. A long process extends across the basal lamina and in between cells of the epidermis to the surface of the foot. The secretory granules travel along the process and their contents are expelled by exocytosis at the foot surface.The contents of the cell body include the nucleus, some endoplasmic reticulum, and an extensive Golgi body with large numbers of secretory vesicles (Fig. 1). The secretory vesicles are membrane bound and contain a fibrillar matrix. At high magnification the similarity of the contents in the Golgi saccules and the secretory vesicles becomes apparent (Fig. 2).

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