scholarly journals Adapting of a Method for Qualitative and Quantitative Determination of Squalene in Distillation Cuts of Sunflower Oil

2021 ◽  
Vol 32 ◽  
pp. 03007
Author(s):  
Irina Dubrovskaya ◽  
Evgeniy Gerasimenko ◽  
Margarita Slobodyanik ◽  
Sergey Sonin
Keyword(s):  
Sample Preparation ◽  
Quantitative Determination ◽  
Sunflower Oil ◽  
Integrated Approach ◽  
Raw Material ◽  
List Type ◽  
Mass Spectral ◽  
Qualitative And Quantitative ◽  
Mass Spectrometric Detector

Squalene is a naturally-occurring dihydro-triterpene hydrocarbon (C30H50) with six double bonds, which is an intermediate in the biosynthesis of phytosterol or cholesterol in plants or animals. The sources of squalene and the main methods forsqualene production and determination are consideredin brief. Sunflower oil distillation cuts have been selected as the subject of the study, since they area promising secondary raw material for the industrial squalene production. The methods of sample preparation and quantification of squalene in sunflower oil distillation cuts applying gas chromatography in combination with mass spectrometry have been adapted. The aim of the studyis to create an integrated approach to determining the qualitative and quantitative content of squalene in distillation cuts of vegetable oils. To achieve the goal of the study, the following tasks have been solved: – Amethod of sample preparation of distillation cuts for determination of squalene has been adapted; – A method of qualitative and quantitative determination of squalene in distillation cuts has been modified. As a result of this study, a technique for sample preparation of distillation cuts was proposed as well as a method for the qualitative and quantitative (absolute calibrationmethod) determination of squalene in distillation cuts of sunflower oil. To implement the technique, a Kristall 5000 gas chromatograph equipped with a mass spectrometric detector was used. Squalene and background components were recorded using the NIST 11 mass spectral database.

scholarly journals Substantiation of technological parameters of the oil extract obtained from Urtica dioica roots based on the yield determination of phytosterols using capillary gas chromatography method

2021 ◽  
pp. 27-36
Author(s):  
Mariana Fedorovska ◽  
Inna Yarema ◽  
Natalia Polovko ◽  
Iryna Ivanchuk
Keyword(s):  
Quantitative Determination ◽  
Sunflower Oil ◽  
Corn Oil ◽  
Urtica Dioica ◽  
Raw Material ◽  
Gas Liquid Chromatography ◽  
Capillary Chromatography ◽  
Optimal Parameters ◽  
Technological Parameters

Due to the content of phytosterols, extractive preparations of Urtica dioica roots are able to show antiandrogenic effect in the case of external therapy of men and women with androgenic alopecia. Oil extracts (OE) are characterized by several advantages when applied to the skin of the scalp compared to water-alcohol extracts. For the development of OE technology from Urtica dioica roots, it is important to choose the optimal extraction parameters, which are based on the quantitative determination of phytosterols in the extractant and the studied samples of extracts. The aim of the work is to choose the optimal parameters for obtaining OE from Urtica dioica roots based on quantitative determination of phytosterols content in experimental samples of OE by gas capillary chromatography. Materials and methods. Objects of the research – Urtica dioica root, refined corn oil, refined sunflower oil, samples of oil extracts. Determination of phytosterol content in experimental samples was carried out by gas capillary chromatography (chromatograph “Crystal 2000”, manufacturer – research and production company “Analytics”). Results. 5 different compounds of steroid structure (stigmasterol, β-sitosterol, etc.) were identified in sunflower oil by gas liquid chromatography, and 10 (campesterol, 2-α stigmasterol, β-sitosterol, Δ5-avenosterol, etc.) were identified in sunflower oil. The quantitative content of β-sitosterol in the sum of sterols of corn oil was significantly higher compared to the content of this substance in sunflower oil and amounted to 59.33 %. Optimal technological parameters were established considering the peculiarities of extraction with oil extractant and quantitative determination of the amount of phytosterols and β-sitosterol in experimental samples of OE. The total content of plant sterols in OE, including considering their amount in the extractant, was in the range of 7880 mg/kg; the amount of β-sitosterol was 4638 mg/kg. Conclusion. The choice of optimal parameters for obtaining OE from UDR based on determination of phytosterol yield by gas capillary chromatography was experimentally substantiated, namely: extractant – corn oil, raw material-extract ratio – 1: 5, extraction time – 6 h, extraction method – maceration

ADAPTING THE METHODOLOGY FOR THE QUANTITATIVE DETERMINATION OF SQUALENE IN SUNFLOWER OIL DISTILLATION CHASES

2021 ◽  
Author(s):  
И.А. ДУБРОВСКАЯ ◽  
Е.О. ГЕРАСИМЕНКО ◽  
М.В. СЛОБОДЯНИК ◽  
С.А. СОНИН ◽  
Н.В. АЛПАТОВА ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Sample Preparation ◽  
Quantitative Determination ◽  
Vegetable Oils ◽  
Wide Spectrum ◽  
Physiological Activity ◽  
Raw Material ◽  
Mass Detection ◽  
Chromatography Method

Погоны дистилляции растительных масел являются перспективным вторичным сырьем для получения сквалена, обладающего широким спектром физиологической активности и являющегося ценным компонентом пищевых продуктов функционального и специализированного назначения, фармпрепаратов и коcметических средств. В настоящее время в Российской Федерации отсутствуют стандартизированные методы количественного определения сквалена в липидных матрицах, в том числе в погонах дистилляции растительных масел, что делает актуальным проведение исследований по адаптации существующих методик для решения данной проблемы. Целью исследования является выбор и адаптация эффективного и доступного метода идентификации и количественного определения сквалена в погонах дистилляции растительных масел. Задачи исследования состоят в разработке методики пробоподготовки, а также в адаптации метода газовой хроматографии для идентификации и количественного определения сквалена в погонах дистилляции растительных масел. В статье рассмотрены природные источники сквалена и основные методы его определения. Предложена методика пробоподготовки и адаптирован метод газовой хроматографии с масс-детектированием для количественного определения сквалена в погонах дистилляции растительных масел. Проведена верификация и определены основные метрологические характеристики метода. Установлено, что предлагаемый метод позволяет определять сквален в погонах дистилляции растительных масел в диапазоне концентраций от 0,1 до 5% с погрешностью не более ± 0,012%. Distillation straps of vegetable oils are a promising secondary raw material for the production of squalene, which has a wide spectrum of physiological activity and is a valuable component of food products for functional and specialized purposes, pharmaceuticals and cosmetics. Currently, in the Russian Federation there are no standardized methods for the quantitative determination of squalene in lipid matrices, including in the distillation strands of vegetable oils, which makes it urgent to carry out studies on the adaptation of existing methods to solve this problem. The aim of the study is to select and adapt an effective, fairly simple and accessible method for the identification and quantitative determination of squalene in distillation strains of vegetable oils. The objectives of the study are to develop a method for sample preparation, as well as to adapt the gas chromatography method for the identification and quantitative determination of squalene in distillation strands of vegetable oils. A method for sample preparation is proposed and the method of gas chromatography with mass detection is adapted for the quantitative determination of squalene in distillation strands of vegetable oils. Verification was carried out and the main metrological characteristics of the method were determined. It has been established that the proposed method makes it possible to determine squalene in distillation straps of vegetable oils in the concentration range from 0,1 to 5% with an error of no more than ± 0,012%.

Qualitative and quantitative determination of trace aldehydes and ketones in food preservative propionic acid for quality improvement

2021 ◽  
Author(s):  
Yiwen Cao ◽  
Shenjie Zhu ◽  
Lin Zhang ◽  
Qun Cui ◽  
Haiyan Wang
Keyword(s):  
Quality Improvement ◽  
Quantitative Analysis ◽  
Quantitative Determination ◽  
Propionic Acid ◽  
Qualitative And Quantitative Analysis ◽  
Food Preservative ◽  
Qualitative And Quantitative ◽  
Aldehydes And Ketones ◽  
Aldehyde Content

Aiming at the difficulty in qualitative and quantitative analysis of trace compositions in food preservative propionic acid, the trace compositions and the key components influencing the total aldehyde content in...

Qualitative and quantitative determination of porphyrins in urine, blood and feces

1987 ◽  
Vol 20 (4) ◽  
pp. 290-291
Author(s):  
M. Moss ◽  
C.S.Y. Wong ◽  
T. McMahon ◽  
C. Cousins ◽  
C. Jacklyn
Keyword(s):  
Quantitative Determination ◽  
Qualitative And Quantitative

Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS

2011 ◽  
Vol 66 (1) ◽  
pp. 102-108 ◽  
Author(s):  
Shihu Wei ◽  
Peicheng Zhang ◽  
Xizhi Feng ◽  
Hiroyuki Kodama ◽  
Changyuan Yu ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Qualitative And Quantitative ◽  
Esi Ms

scholarly journals Determination of Free and Bound Maleic Hydrazide Residues in Tobacco by High Performance Liquid Chromatography

1994 ◽  
Vol 16 (2) ◽  
pp. 57-64 ◽  
Author(s):  
BS Dattilo ◽  
S Gallo ◽  
G Lionetti ◽  
SG Rossi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
High Performance ◽  
Maleic Hydrazide ◽  
Dilute Solution ◽  
Tobacco Leaves ◽  
Qualitative And Quantitative ◽  
Curve Method

AbstractA new method is described for the qualitative and quantitative determination of both free and bound maleic hydrazide residues in tobacco leaves and cigarette filler by high performance liquid chromatography. Analyses were carried out by hydrolyzing samples of ground tobacco with 4 N hydrochloric acid for 40 minutes under reflux followed by sample chromatography, running isocratic elutions with a dilute solution of phosphoric acid. The quantitative determination of maleic hydrazide was performed by light absorption at 320 nm, by the calibration curve method. Recoveries of maleic hydrazide added to tobacco samples were greater than 90 %. The detection limit of the method, determined on ground tobacco leaves, was at least 5 ppm. The results obtained by this procedure and by the ISO standard method no. 4876 are in good accordance.

Qualitative and quantitative determination of major saponins in Paris and Trillium by HPLC-ELSD and HPLC–MS/MS

2010 ◽  
Vol 878 (29) ◽  
pp. 2943-2948 ◽  
Author(s):  
Shuli Man ◽  
Wenyuan Gao ◽  
Yanjun Zhang ◽  
Jieyin Wang ◽  
Wanshun Zhao ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Qualitative And Quantitative
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