scholarly journals Efficacy of Incorporation Platelet Rich Plasma into Gelatine Hydrogel Scaffold between Impregnated and Drop Method

2021 ◽  
Vol 41 ◽  
pp. 05002
Author(s):  
Erlina Sih Mahanani ◽  
Fannisa Afrilyana Ulzanah

Tissue Engineering which involve three main component such as scaffold, platelet-rich plasma (PRP) and cells is expected to support in bone regeneration. Gelatin hidrogel scaffold is planted have a function as cell environment and PRP provide growth factor to support differentiation of cells. The success of tissue engineering is affected by number of PRP which is contained in scaffold. The purpose of this study is to compare the incorporation process between impregnated and drop method to gelatin hidrogel scaffold. PRP was prepared from three donors of whole blood, and twice sentrifugation by 450 rcf for 5 minutes and 1500 rcf for 7 minutes. PRP was incorporated into 3 gelatin hidrogel scaffolds for each methods. The remnant of PRP which didn’t incorporate were calculated the number of platelet with giemsa stainning. Platelet which loaded were the reduction result of number platelet before incorporate with platelet remnant. Data of the result were analyzed using independent sample t test. Result show the significant was 0.262 (p>0.05) there’s no significane different between impregnated and drop method for incorporating PRP into gelatin hidrogel scaffold. The number of platelet which incorporated in gelatin hidrogel scaffold were effected by characteristic of scaffold such as structure, interface adherence, porosity and swelling ability. The good characteristic of scaffold could be obtain from synthesis and good fabrication technique.

2016 ◽  
Vol 22 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Jagoda M. Jalowiec ◽  
Matteo D'Este ◽  
Jennifer Jane Bara ◽  
Jessica Denom ◽  
Ursula Menzel ◽  
...  

2016 ◽  
Vol 105 ◽  
pp. 249-263 ◽  
Author(s):  
Yang Song ◽  
Can Yu ◽  
Chunli Wang ◽  
Xingshuang Ma ◽  
Kang Xu ◽  
...  

Gels ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 44
Author(s):  
Marfa N. Egorikhina ◽  
Yulia P. Rubtsova ◽  
Diana Ya. Aleynik

The most difficult issue when using tissue engineering products is enabling the ability to store them without losing their restorative capacity. The numbers and viability of mesenchymal stem cells encapsulated in a hydrogel scaffold after cryostorage at −80 °C (by using, individually, two kinds of cryoprotectors—Bambanker and 10% DMSO (Dimethyl sulfoxide) solution) for 3, 6, 9, and 12 months were determined, with subsequent assessment of cell proliferation after 96 h. The analysis of the cellular component was performed using fluorescence microscopy and the two fluorochromes—Hoechst 3334 and NucGreenTM Dead 488. The experimental protocol ensured the preservation of cells in the scaffold structure, retaining both high viability and proliferative activity during storage for 3 months. Longer storage of scaffolds led to their significant changes. Therefore, after 6 months, the proliferative activity of cells decreased. Cryostorage of scaffolds for 9 months led to a decrease in cells’ viability and proliferative activity. As a result of cryostorage of scaffolds for 12 months, a decrease in viability and proliferative activity of cells was observed, as well as pronounced changes in the structure of the hydrogel. The described scaffold cryostorage protocol could become the basis for the development of storage protocols for such tissue engineering products, and for helping to extend the possibilities of their clinical use while accelerating their commercialization.


Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 879
Author(s):  
Anton Manakhov ◽  
Elizaveta Permyakova ◽  
Sergey Ershov ◽  
Svetlana Miroshnichenko ◽  
Mariya Pykhtina ◽  
...  

The immobilization of viable proteins is an important step in engineering efficient scaffolds for regenerative medicine. For example, angiogenin, a vascular growth factor, can be considered a neurotrophic factor, influencing the neurogenesis, viability, and migration of neurons. Angiogenin shows an exceptional combination of angiogenic, neurotrophic, neuroprotective, antibacterial, and antioxidant activities. Therefore, this protein is a promising molecule that can be immobilized on carriers used for tissue engineering, particularly for diseases that are complicated by neurotrophic and vascular disorders. Another highly important and viable protein is apoliprotein A1. Nevertheless, the immobilization of these proteins onto promising biodegradable nanofibers has not been tested before. In this work, we carefully studied the immobilization of human recombinant angiogenin and apoliprotein A1 onto plasma-coated nanofibers. We developed a new methodology for the quantification of the protein density of these proteins using X-ray photoelectron spectroscopy (XPS) and modeled the XPS data for angiogenin and apoliprotein A1 (Apo-A1). These findings were also confirmed by the analysis of immobilized Apo-A1 using fluorescent microscopy. The presented methodology was validated by the analysis of fibronectin on the surface of plasma-coated poly(ε-caprolactone) (PCL) nanofibers. This methodology can be expanded for other proteins and it should help to quantify the density of proteins on surfaces using routine XPS data treatment.


2015 ◽  
Vol 6 (2) ◽  
pp. 184-194 ◽  
Author(s):  
Ethan Nyberg ◽  
Christina Holmes ◽  
Timothy Witham ◽  
Warren L. Grayson

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