scholarly journals DNA Barcoding of Swamp Sediment Bacterial Isolates for Swamp Aquaculture Probiotic

2018 ◽  
Vol 68 ◽  
pp. 01023
Author(s):  
Marini Wijayanti ◽  
Dade Jubaedah ◽  
Januar Ahlan Suhada ◽  
Siti Yuliani ◽  
Nabilah Saraswati ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Gene Bank ◽  
Bacillus Sp ◽  
Dna Barcodes ◽  
Bacterial Isolates ◽  
Streptomyces Sp ◽  
Blast Analysis ◽  
16S R Rna ◽  
Aquaculture Ponds ◽  
Rna Genes

Bacteria derived from swamp sediments and aquaculture ponds potential to be probiotics. The study aims to determine the sequence of 16S r RNA gene of isolate of probiotics candidate bacteria from sediment of swamp, to determine the phylogenetic tree between the bacterial species from isolates and Gene Bank data central for their potential as probiotic. The samples of bacteria resulted from pure isolation selected from the sediment of pond cultivation and swamp waters at Lebung Karangan Reservation, Ogan Ilir Regency, Indralaya, South Sumatra. This study was started from cultivating bacteria, extracting DNA of bacteria, amplification 16S r RNA genes by PCR, running electrophoresis, and sequencing the amplicon for determining DNA barcodes of bacteria from sediment of swamp and rearing pond. The result of BLAST analysis showed that KA isolate had the highest similarity 97% with Streptomyces sp. Hjorring101 from Denmark and RA isolate had the highest similarity 98% with Streptomyces sp. BD99 from Pakistan. KE isolate had the highest similarity 99% with Bacillus subtilis CESi5 from Japan and RE isolate had the highest similarity 93% with Bacillus sp.2bFR from Manado. All of isolates were bacteria potentially as swamp aquaculture probiotics.

scholarly journals Diversity of Biofilm-Forming Bacteria in Chinnamuttom Harbor of Southeast India

2021 ◽  
Vol 14 (4) ◽  
pp. 1793-1799
Author(s):  
P. Nithya
Keyword(s):  
Surface Water ◽  
Bacterial Species ◽  
Ship Hull ◽  
Rrna Gene ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Four Seasons ◽  
The Mean ◽  
Marine Environmental

Biofilms are species rich, partially due to highly effective powers of diffusion of the microorganisms and have wide tolerance to marine environmental conditions. Characteristically, the first organisms to respond to and convalesce from stress. The present study aims to isolate and identify the biofilm forming bacterial species, collected from surface water and substratum of the ship hull for four seasons at Chinnamuttom fishing harbor, Southeast coast of India, during June 2015 to May 2016. Among the mean concentration of bacterial isolates of both water and substratum of the station, maximum in monsoon and minimum in summer seasons. Totally 16 isolates were obtained, based on the adherence property, 8 isolates from surface water and 8 isolates from substratum of the ship hull. The samples were plated on Zobell marine agar medium for bacterial isolates of study area. The isolates of Bacillus sp., Flavobacterium sp., Pseudomonas sp., Aeromonas sp., Micrococcus sp., Vibrio sp., Salmonella sp., Staphylococcus sp., Shegella sp., Klebsiella sp., Corynebacterium sp., Enterobacter sp., Chromohalobacter sp., Bacillus sp., Escherichia coli and Bacilus sp. were reported in all the seasons at study area. The major diverse bacterial isolates were further characterized through morphological and molecular identification. Based on 16S rRNA gene sequences. Biofilm bacterial isolates were confirmed as Bacillus sp., and Pseudomonas sp.

DIVERSITY OF CULTURABLE BACTERIAL MICROBIOTA OF THE Eisenia foetida DIGESTIVE TRACT

2018 ◽  
Vol 41 (3) ◽  
pp. 255-264 ◽  
Author(s):  
J. Abraham Pérez-Pérez ◽  
David Espinosa-Victoria ◽  
Hilda V. Silva-Rojas ◽  
Lucía López-Reyes
Keyword(s):  
Bacterial Diversity ◽  
Digestive Tract ◽  
Bacterial Species ◽  
Brain Heart Infusion ◽  
Rrna Gene ◽  
Eisenia Foetida ◽  
Bacterial Isolates ◽  
Bacterial Microbiota ◽  
Decomposition Of Organic Matter ◽  
Earthworm Gut

Bacteria are an unavoidable component of the natural earthworm diet; thus, bacterial diversity in the earthworm gut is directly linked to decomposition of organic matter and development of the surrounding plants. The aim of this research was to isolate and to identify biochemically and molecularly the culturable bacterial microbiota of the digestive tract of Eisenia foetida. Earthworms were sourced from Instituto de Reconversión Productiva y Bioenergética (IRBIO) and Colegio de Postgraduados (COLPOS), México. Bacterial isolation was carried out on plates of Brain Heart Infusion (BHI) culture medium. Fifty six and 44 bacterial isolates were obtained from IRBIO and COLPOS, respectively. The population was composed of 44 Gram-negative and 56 Gram-positive isolates. Over 50 % of the bacterial isolates were rod-shaped cells. The 16S rRNA gene was sequenced and nine genera were identified in worms from IRBIO (Bacillus, Paenibacillus, Solibacillus, Staphylococcus, Arthrobacter, Pantoea, Stenotrophomonas, Acinetobacter and Aeromonas) and six in worms from COLPOS (Bacillus, Paenibacillus, Stenotrophomonas, Staphylococcus, Acinetobacter and Aeromonas). Bacillus was the predominant genus, with eight and six species in the oligochaetes from IRBIO and COLPOS, respectively. The most represented bacteria in the worms from both sites were Bacillus sp. and B. subtilis. The predominance of Bacillus was probably due to spore formation, a reproductive strategy that ensures survival and dispersion in the soil and oligochaetes digestive tract. The gut of E. foetida not only harbored bacterial species of agronomic importance but also species potentially pathogenic for humans (Staphylococcus warneri, Pantoea agglomerans and Stentrophomonas sp.). The larger bacterial diversity in worms from IRBIO could be due to their feeding on cattle manure, which is a rich source of bacteria.

scholarly journals Virulent secondary metabolites of entomopathogenic bacteria genera, Xenorhabdus and Photorhabdus, inhibit phospholipase A2 to suppress host insect immunity

2020 ◽  
Author(s):  
Md. Mahi Imam Mollah ◽  
Yonggyun Kim
Keyword(s):  
Secondary Metabolites ◽  
Bacterial Culture ◽  
Bacterial Species ◽  
Culture Broth ◽  
Insect Immunity ◽  
Bacterial Isolates ◽  
Bacterial Pathogenicity ◽  
Entomopathogenic Bacteria ◽  
Organic Extracts ◽  
Insecticidal Activities

Abstract Background: Xenorhabdus and Photorhabdus are entomopathogenic bacteria that cause septicemia and toxemia in insects. They produce secondary metabolites to induce host immunosuppression. Their metabolite compositions vary among bacterial species. Little is known about the relationship between metabolite compositions and the bacterial pathogenicity. The objective of this study was to compare pathogenicity and production of secondary metabolites of 14 bacterial isolates (species or strains) of Xenorhabdus and Photorhabdus. Results: All bacterial isolates exhibited insecticidal activities after hemocoelic injection to Spodoptera exigua (a lepidopteran insect) larvae, with median lethal doses ranging from 168.8 to 641.3 CFU per larva. Bacterial infection also led to immunosuppression by inhibiting eicosanoid biosynthesis. Bacterial culture broth was fractionated into four different organic extracts. All four organic extracts of each bacterial species exhibited insecticidal activities and resulted in immunosuppression. These organic extracts were subjected to GC-MS analysis which predicted 182 compounds, showing differential compositions for 14 bacteria isolates. There were positive correlations between total number of secondary metabolites produced by each bacterial culture broth and its bacterial pathogenicity based on immunosuppression and insecticidal activity. From these correlation results, 70 virulent compounds were selected from secondary metabolites of high virulent bacterial isolates by deducting those of low virulent bacterial isolates. These selected virulent compounds exhibited significant immunosuppressive activities by inhibiting eicosanoid biosynthesis. They also exhibited relatively high insecticidal activities. Conclusion: Virulence variation between Xenorhabdus and Photorhabdus is determined by their different compositions of secondary metabolites, of which PLA2 inhibitors play a crucial role.

scholarly journals Detection of Polymorphism in the Gene blaKPC2 of Local Klebsiella pneumoniae Isolated from Iraqi Patients

2017 ◽  
Vol 11 (1) ◽  
pp. 26-33
Author(s):  
Saadi Abd-Alkareem Jasim ◽  
Mohammed Maaroof ◽  
Najwa Ahmed
Keyword(s):  
Environmental Management ◽  
Klebsiella Pneumoniae ◽  
Dna Sequences ◽  
Microscopic Examination ◽  
Gene Bank ◽  
Bacterial Isolates ◽  
Wild Type ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Severe Infections

Carbapenemases are clinically important because they destroy and may confer a resistance to carbapenems, severe infections caused by carbapenemase producers is associated with increased mortality. To achieve this goal, 180 samples were collected from different clinical sources included 92 urine, 33 smears of wounds, 13 smears of burns and 42 sputum. The samples were taken from patients attended Al-Yarmouk Teaching Hospital and Ibin Baladi Hospital in Baghdad Governorate. Diagnosis of bacterial isolates was done depending upon the microscopic examination, the cultured characteristics and biochemical tests. DNA extracted from 84 samples. Accordingly, detection of blaKPC2 gene was conducted by using specific primers for amplification of blaKPC2 gene. Moreover, the sequencing of 910 bp for blaKPC2 gene was performed by the biotechnology lab. at the National Instrumentation Center for Environmental Management (NICEM). Such test has been implanted by using 3730XL as a DNA sequences. The obtained results were analyzed by blast at the National Center Biotechnology Information (NCBI) and detect polymorphism in blaKPC2 based on the Bio Edit. Consequently, 94 variations between 47 transversions, 43 transitions and 4 deletions nucleotide were noticed. In a sense the test showed 79% under sequence ID gb|CP009872.1| from 3037673 -3038166 number of nucleotide from K. pneumoniae subsp. pneumoniae strain KPNIH30 of Gene Bank, score (329) and expect 5e-86 with the wild type of blaKPC2 gene from Gene Bank. Finally, the results illustrated polymorphism between local strains of

Isolation, Characterization and Application of Protease Enzyme from Marine Bacteria

2021 ◽  
pp. 4236-4240
Author(s):  
Sneha S ◽  
Mrunal Palsokar ◽  
Vemula Sai Jahnavi ◽  
Anwesha Sarkar ◽  
K. V. Bhaskara Rao
Keyword(s):  
Marine Bacteria ◽  
Peptide Bond ◽  
Mangrove Sediment ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Sediment Samples ◽  
Protease Enzyme ◽  
Stain Removal ◽  
Marine Sediment Sample ◽  
Protease Assay

Protease constitutes the major group of catalytic enzymes which is involved in hydrolyzing peptide bond of proteins. Marine sediment sample were collected and protease producing bacterial isolates were identified by using casein as a substrate. The organisms were characterized by biochemical test and identified as Bacillus sp. In order to check for the production of protease enzyme, quantitative protease assay and Lowry’s method of protein estimation was carried out. The crude extract of protease was subjected for blood stain removal activity and the enzyme proved to be efficient which removed the stain in 15 min. The purpose of the current study is to isolate, identify, characterize and to carry out applications of protease enzyme from marine bacteria isolated from mangrove sediment samples.

scholarly journals Isolation, Characterization, and Identification of Bacterial Population from Textile and Tannery Effluents of Bangladesh

2016 ◽  
Vol 29 (2) ◽  
pp. 84-88
Author(s):  
A Hakim ◽  
S Hoque ◽  
SM Ullah
Keyword(s):  
Bacterial Population ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Tannery Effluents ◽  
Bacterial Composition ◽  
Degrading Bacteria ◽  
Diagnostic Characteristics ◽  
Metal Treatment

Ten effluent samples from two different sites located at Hazaribagh tannery belt and Dhaka EPZ, Savar were collected. This study aimed to compare the bacterial composition isolated from tannery and textile effluents and to investigate the occurrence of metal toxicity tolerant and dye degrading bacteria and to select the potential strains for the use in bioremediation. The average bacterial count of HT and DETDE varied in between 3.35×106 and 5.45×106 cfu/mL and 4.8×106 and 7.75×106cfu/mL, respectively. A total of 12 bacterial isolates were characterized as strains of Bacillus, Staphylococcus, and Pseudomonas. A few, however, were re-cultured on other recommended media for verification of diagnostic characteristics. Maximum numbers of bacterial species were isolated from textile effluent. The results showed that a Gram-positive bacillus with a yellow pigment was considered as a major group of the population. Among them three isolates were identified based on alignments of partial sequence of 16S rRNA gene. These are also being used in different wastewater and metal treatment plants all over the world.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 84-88

Screening of new bacterial isolates with antifungal activity and application of selected Bacillus sp. cultures for biocontrol of Fusarium graminearum under field conditions

2018 ◽  
Vol 113 ◽  
pp. 22-28 ◽  
Author(s):  
Karina Stumbriene ◽  
Renata Gudiukaite ◽  
Roma Semaskiene ◽  
Povilas Svegzda ◽  
Akvile Jonaviciene ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Graminearum ◽  
Field Conditions ◽  
Bacillus Sp ◽  
Bacterial Isolates

scholarly journals Influence of the Diversity of Bacterial Isolates from Drinking Water on Resistance of Biofilms to Disinfection

2010 ◽  
Vol 76 (19) ◽  
pp. 6673-6679 ◽  
Author(s):  
Lúcia Chaves Simões ◽  
Manuel Simões ◽  
Maria João Vieira
Keyword(s):  
Drinking Water ◽  
Sodium Hypochlorite ◽  
Bacterial Species ◽  
Acinetobacter Calcoaceticus ◽  
Single Species ◽  
Bacterial Isolates ◽  
Multispecies Biofilms

ABSTRACT Single- and multispecies biofilms formed by six drinking water-isolated bacterial species were used to assess their susceptibilities to sodium hypochlorite (SHC). In general, multispecies biofilms were more resistant to inactivation and removal than single biofilms. Total biofilm inactivation was achieved only for Acinetobacter calcoaceticus single-species biofilms and for those multispecies biofilms without A. calcoaceticus. Biofilms with all bacteria had the highest resistance to SHC, while those without A. calcoaceticus were the most susceptible. A. calcoaceticus formed single biofilms susceptible to SHC; however, its presence in multispecies biofilms increased their resistance to disinfection.

scholarly journals Production of Invertase from Actinomycetes Isolated from Soils in Eliogbolo and Oyigbo, Rivers State using Submerged Fermentation Technique

2019 ◽  
pp. 1-8
Author(s):  
T. O. Ozoude ◽  
O. K. Agwa ◽  
E. C. Wokoma
Keyword(s):  
Yeast Extract ◽  
Bacterial Species ◽  
Sugar Content ◽  
Research Work ◽  
Pharmaceutical Formulations ◽  
Botanical Garden ◽  
Reducing Sugar ◽  
The Other ◽  
Lag Phase ◽  
Streptomyces Sp

The use of commercial sources of invertase for the production of High fructose syrup and other pharmaceutical formulations has been a problem due to its high ash content and cost implication, therefore there is a need to explore bacterial species that can produce invertase. In this study, actinomycetes strains were isolated from soils from a botanical garden, sugar cane sites and garden egg at Elogbolo and Oyigbo respectively. Yeast extract agar; Czapeks Dox agar and Arginine glycerol agar were used for the isolation of actinomycetes. The study showed that Czapeks Dox agar gave the highest population count followed by Yeast extract and Arginine glycerol agar. The dominated genus of actinomycetes observed were species of Streptomyces designated as OZMU 1; OZMU 2; OZMU 3 and OZMU 4. The results showed that the reducing sugar content of the Streptomyces sp. had similar reducing sugar liberation and consumption behavior without any lag phase, with OZMU 1 concentration peak at 12 h (0.12 g/l), and OZMU 3 (0.15 g/l).  But OZMU 2 indicated a lag phase from 6-12h, increased from 12-24 h (0.18 g/l), but activity declined dramatically at 36 h and 48 h. The reducing sugar content for Streptomyces sp. OZMU 4 revealed longer lag phase, which was almost constant throughout the investigation period from 6-36 h. But at 48 h (0.06 g/l)  a decline in the reducing sugar content was noticed compared to the other isolates, indicating that all the available reducing sugar were completely consumed by the other strains. The research work revealed the ability of indigenous actinomycetes strains to produce invertase enzyme which has high conversion efficiency and can be used in various pharmaceutical and nutraceutical industries.

scholarly journals Virulent secondary metabolites of entomopathogenic bacteria genera, Xenorhabdus and Photorhabdus, inhibit phospholipase A2 to suppress host insect immunity

2020 ◽  
Author(s):  
Md. Mahi Imam Mollah ◽  
Yonggyun Kim
Keyword(s):  
Secondary Metabolites ◽  
Bacterial Culture ◽  
Bacterial Species ◽  
Culture Broth ◽  
Insect Immunity ◽  
Bacterial Isolates ◽  
Bacterial Pathogenicity ◽  
Entomopathogenic Bacteria ◽  
Organic Extracts ◽  
Insecticidal Activities

Abstract Background: Xenorhabdus and Photorhabdus are entomopathogenic bacteria that cause septicemia and toxemia in insects. They produce secondary metabolites to induce host immunosuppression. Their metabolite compositions vary among bacterial species. Little is known about the relationship between metabolite compositions and the bacterial pathogenicity. The objective of this study was to compare pathogenicity and production of secondary metabolites of 14 bacterial isolates (species or strains) of Xenorhabdus and Photorhabdus. Results: All bacterial isolates exhibited insecticidal activities after hemocoelic injection to Spodoptera exigua (a lepidopteran insect) larvae, with median lethal doses ranging from 168.8 to 641.3 CFU per larva. Bacterial infection also led to immunosuppression by inhibiting eicosanoid biosynthesis. Bacterial culture broth was fractionated into four different organic extracts. All four organic extracts of each bacterial species exhibited insecticidal activities and resulted in immunosuppression. These organic extracts were subjected to GC-MS analysis which predicted 182 compounds, showing differential compositions for 14 bacteria isolates. There were positive correlations between total number of secondary metabolites produced by each bacterial culture broth and its bacterial pathogenicity based on immunosuppression and insecticidal activity. From these correlation results, 70 virulent compounds were selected from secondary metabolites of high virulent bacterial isolates by deducting those of low virulent bacterial isolates. These selected virulent compounds exhibited significant immunosuppressive activities by inhibiting eicosanoid biosynthesis. They also exhibited relatively high insecticidal activities.Conclusion: Virulence variation between Xenorhabdus and Photorhabdus is determined by their different compositions of secondary metabolites, of which PLA2 inhibitors play a crucial role.

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