scholarly journals Applying methods of replication and recovery of potato microplants (Solanum tuberosum l.) in seed production

2020 ◽  
Vol 203 ◽  
pp. 02003
Author(s):  
Irina Kim ◽  
Elena Barsukova ◽  
Petr Fisenko ◽  
Tatyana Chekushkina ◽  
Alena Chibizova ◽  
...  
Keyword(s):  
Plant Breeding ◽  
Seed Production ◽  
Viral Infections ◽  
Solanum Tuberosum L ◽  
Far East ◽  
Potato Spindle Tuber Viroid ◽  
Propagation Rate ◽  
Potato Leaf ◽  
Potato Varieties

Potatoes are strongly affected by pests and by pathogens of fungal, bacterial and viral nature. The most common and economically significant potato viruses are Y (PVY), X (PVX), S (PVS), M (PVM), and potato leaf twisting virus (PLRV). The development of a virus-free bio-resource collection in vitro is the basis for plant breeding development and transferring seed production to a healthier foundation. In this regard, the aim of this research was to apply methods of recovery and select optimal conditions for in vitro propagation of a collection of virus-free potato varieties. A collection of 22 healthy virus-free potato varieties was developed and kept in vitro in the FSBSI “FSC of Agricultural Biotechnology of the Far East named after A. K. Chaika". The recovery from viruses through joint use of tissue culture (apexes 2-4 mm) and chemotherapy (ribavirin) of the new potato variety Avgustin was carried out. The recovered test-tube plants, as well as the samples of six in vitro potato varieties that are in demand in plant breeding and seed production (Smak, Sante, Yantar, Zhukovsky ranny, Dachny, Adretta), were tested by enzyme immunoassay method (ELISA) for latent infection with viruses Y (PVY), X (PVX), S (PVS), M (PVM), and L (PLRV). The evaluation for Potato Spindle Tuber Viroid (PSTVd) was performed using PCR method. As a result of the study, no viral infections were detected in the recovered material and plants in vitro. The composition of nutrient medium for the microclonal propagation of potatoes that provides maximum value of the propagation rate is detected.

The Study of Rhizogenes of Potato Varieties (Solanum tuberosum L.) in vitro

2013 ◽  
pp. 69-72 ◽  
Author(s):  
Ksenia Guseva ◽  
Irina Borodulina ◽  
Elena Myakisheva ◽  
Otari Tavartkiladze
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Potato Varieties

scholarly journals Micropropagation of Environmental Stress Tolerant Local Potato (Solanum tuberosum L.) Varieties of Bangladesh

2014 ◽  
Vol 24 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Fazlima Parveen ◽  
Mahmuda Khatun ◽  
Aparna Islam
Keyword(s):  
Shoot Apex ◽  
Large Scale ◽  
Solanum Tuberosum L ◽  
In Vitro Regeneration ◽  
Nodal Explants ◽  
Direct Regeneration ◽  
Nodal Segment ◽  
Scale Production ◽  
Potato Varieties

In vitro response of four explants namely, leaf, shoot apex, nodal and internodal, in three stress tolerant Bangladeshi potato varieties, viz. Zaubilati, Shadaguti and Challisha were tested. Of all the varieties, Shadaguti responded best for all the explants. Among the explants nodal segment responded best, followed by shoot apex. For all these explants and varieties, shoot regeneration response was tested in response to two cytokines, BAP and Kn. When compared between BAP and Kn supplementation, Kn treatment performed better than that of BAP for nodal and shoot apex, while opposite was observed for remaining explants. Interestingly, hormone free basal PROP medium was found to be best for nodal explants of both Zaubilati and Challisha varieties. While nodal explants of Shadaguti showed the best result in 0.5 mg/l Kn. Shoot apex  performed best in 0.5 mg/l Kn  for all the varieties. As an explant both internode and leaf did not perform well. Direct regeneration from these explants was found best in PROP + 0.2 mg/l GA3 + 0.5 mg/l IAA;  1.0 mg/l BAP, 1.5 mg/l BAP and 2.0 mg/l BAP for Zaubilai, Challisha and Shadaguti, respectively. For rooting, of the in vitro grown shoots half strength of PROP medium supplemented with 0.5 mg/l IBA was found to be best. Cent percent survival of transplanted plantlets was recorded. The successful protocol for in vitro regeneration was developed which can be used for large scale production of these abiotic stress tolerant potato varieties. Plant Tissue Cult. & Biotech. 24(1): 101-109, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19218

scholarly journals Effect of the potato virus PVS0 as a biotic stress factor in plants of Siberian potato varieties (Solanum tuberosum L) on the level of soluble peroxidase enzymes

2020 ◽  
Vol 132 (3) ◽  
pp. 28-42
Author(s):  
I.V. Kirgizova ◽  
◽  
S.B. Chachina ◽  
Keyword(s):  
Solanum Tuberosum ◽  
Environmental Factors ◽  
Biotic Stress ◽  
Western Siberia ◽  
Viral Infections ◽  
Solanum Tuberosum L ◽  
Potato Varieties ◽  
Potato Plants ◽  
Soluble Peroxidase ◽  
Peroxidase Enzymes

. Currently, domestic potato varieties are affected by phytopathogenic viral infections, which lead to a decrease in yield up to 80%, therefore. Therefore, it is relevant to study the system of protecting potato plants from biotic environmental factors. The aim of the work is to study the levels of the antioxidant enzyme peroxidase in response to the effect of the viral infection PVS, which is the least studied and widespread infection in Western Siberia

scholarly journals In vitro micropropagation and ex vitro rooting of some potato varieties

2019 ◽  
Vol 9 (4) ◽  
pp. 679-689
Author(s):  
D. A. Durnikin ◽  
N. A. Kolpakov ◽  
K. Y. Guseva ◽  
A. V. Matsyura
Keyword(s):  
Acetic Acid ◽  
Nutrient Medium ◽  
Solanum Tuberosum L ◽  
Ex Vitro ◽  
Potato Varieties ◽  
Clonal Micropropagation ◽  
In Vitro Micropropagation ◽  
Indolyl Acetic Acid ◽  
To Receive

The article presents the results on selection of optimal concentrations of nutrient media components and nutrient solution at the stages of clonal micropropagation of potato varieties Lyubava, Kemerovochanin, and Tuleevskiy (actual reproduction, rooting in vitro, adaptation to ex vitro conditions). The influence of some components of the nutrient medium (sucrose, agar-agar, growth regulators, namely α-naphthyl acetic acid, β-indolyl acetic acid, and β-indolyl propionic acid) was studied at the stages of reproduction and rooting in order to obtain regenerants of the studied potato varieties. The best development of plants on nutrient medium with addition of 4 g L-1 of agar-agar was revealed. The addition of sucrose in the concentration of 3-5% contributed to the formation of more internodes. The influence of naphthyl acetic acid, β-indolyl oil, and β-indolyl propionic acids in different concentrations on the rhizogenesis of regenerating plants of three potato varieties at the stages of reproduction and establishment of different types and concentrations of auxins was studied. The researches have shown that a one-stage method of adaptation of Solanum tuberosum L. varieties of Lyubava, Kemerovochanin, and Tuleevskiy to the conditions of ex vitro cultivation with the use of hydroponic installation is characterized by efficiency and allows to receive plants with well-developed root system. The efficiency of using the hydroponic plant for adaptation of potato regenerants at the final stage of micropropagation is shown.

Continous replication of potato spindle tuber viroid (PSTV) in permanent cell cultures of potato and tomato

1981 ◽  
Vol 1 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Hans-Peter Mühlbach ◽  
Heinz L. Sänger
Keyword(s):  
De Novo ◽  
Solanum Tuberosum L ◽  
Polyacrylamide Gel Electrophoresis ◽  
Potato Spindle Tuber Viroid ◽  
Callus Cultures ◽  
Wild Type ◽  
Infectivity Assay ◽  
Permanent Cell ◽  
One Year

The continuous replication of potato spindle tuber viroid (PSTV) in callus cultures from PSTV-infected wild-type potato (Solanum dem/ssum L.) and tomato (Lycopersicon peruvianum L. Mill) plants and in cell suspensions derived from potato protoplasts (Solanum tuberosum L.) inoculated in vitro is described. The persistence of PSTV replication in these cell lines through at least 14 subculture passages, which corresponds to a continous replication over a period of more than one year, was demonstrated by infectivity assay and by polyacrylamide-gel electrophoresis of isolated nucleic acids. This continuous synthesis de novo of PSTV was substantiated by the incorporation of [3H]uridine and of [32P]orthophosphate into viroid RNA.

Utilization of Shoot Cuttings for Elimination of PLRV and PVY by Thermotherapy and Chemotherapy from Potato (Solanum tuberosum L.)

2007 ◽  
Vol 7 ◽  
pp. 1
Author(s):  
S. P. Dhital ◽  
B. M. Sakha ◽  
H. T. Lim
Keyword(s):  
Plant Height ◽  
Solanum Tuberosum L ◽  
Combined Treatment ◽  
Leaf Roll ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potato Leaf ◽  
Virus Elimination ◽  
Elisa Testing ◽  
Das Elisa

Heat and ribavirin treatments were applied for the elimination of potato leaf roll virus (PLRV) and potato virus Y (PVY) from the potato genotype F 9-99. The explants, rooted young plantlets, cultured on MS medium with and without ribavirin (20 mg l<sup>-1</sup>) were subjected to thermotherapy (35ºC/31ºC, 4 h alternating periods) and room temperature (25ºC) for 30 days. Double antibody sandwich - enzyme linked immunosorbent assay (DAS-ELISA) testing following the therapies revealed that ribavirin alone was enable to eliminate 10% each of PLRV and PVY, whereas along with thermotherapy its efficacy increased to 25% PLRV and 20% PVY elimination. In another experiment, three potato genotypes F 9-99 infected with PLRV and PVY, Gui Valley with PLRV infection and Rose Valley with PVY infection were evaluated. Ribavirin (20 mg l<sup>-1</sup>) and ASA (acetylsalicylic acid) (10<sup>-5</sup> M) were supplemented in liquid culture medium alone or in combination with thermotherapy for three successive cycles of 30-36 days interval. Heat and/or ribavirin suppressed survival and plant height whereas ASA promoted the survival as well as plant height even under heat treatment. After each cycle, the effect of treatment on virus elimination was evaluated by DAS-ELISA. The combined application of ribavirin and ASA with thermotherapy after three cycles of treatment showed up to 47.4% PLRV and 57.9% PVY elimination in F 9-99. In the case of single viral infection of PLRV or PVY the same combined treatment showed up to 58.8% PLRV and 61.1% PVY free plantlets. Virus elimination was also confirmed by transplanting in vitro grown plantlets in a net house and then retesting after 45 days of in vivo growth. This novel technique would be highly efficient for virus elimination within a short duration in diverse genotypes of potato. <i>Nepal Journal of Science and Technology </i> 7 2006

Effect of Kinetin on Growth and Development of Microtubers of Potato (Solanum Tuberosum L.)

2021 ◽  
Vol 1 (4) ◽  
pp. 48-54
Author(s):  
Kumari Meenakshi ◽  
Keyword(s):  
Solanum Tuberosum ◽  
Growth And Development ◽  
Solanum Tuberosum L ◽  
Shoot Length ◽  
Central Research ◽  
Fresh Weight ◽  
Potato Varieties ◽  
Yield Parameters

The present study was conducted at Central Research Potato Institute, Campus, Modipuram, India to assess the effect of kinetin on the growth and development of microtubers formation in vitro in two potato varieties Kufri Bahar and Kufri Surya. The lower concentration of kinetin (0.75 mg/l) showed decreased parameters like the number of microtubers, fresh weight of microtubers, eyes per microtuber. Higher concentration of kinetin (1.5 mg/l and 2.25 mg/l) led to increasing parameters as compared to control but decreased shoot length in both the varieties but kinetin at (2.25 mg/l) concentration gave best results for in vitro microtuberization for quality and yield parameters.

scholarly journals Interactive effect of genotype and medium on microtuberization of potatoes (Solanum tuberosum L.) grown in vitro

2020 ◽  
Vol 7 (1) ◽  
pp. 8-25
Author(s):  
Kadi Zahia ◽  
Rached Kanouni Malika ◽  
Khammar Sara ◽  
Chebout Abderrezeq ◽  
Khammar Hichem ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
First Generation ◽  
Culture Media ◽  
Solanum Tuberosum L ◽  
Interactive Effect ◽  
Potato Varieties ◽  
Morphological Aspects ◽  
Growing Conditions

AbstractThis work investigates the interactive effect of six culture media and three photoperiods (darkness, 16h/8h, 8h/16h) on the microtuberization of four potato varieties (Spunta, Désirée, Kondor and Bartina). The objective is to determine the best tuberization under these growing conditions. The measured parameters which are related to the suitability of the tuberization characteristics are: the morphological aspects (shape, position) and the biometric ones (number and diameter of tubers). The obtained results permit to valorize the meristems that constitute the starting explant, and their good organogenetic skills to provide the first generation of micropropagation with healthy vitroplants in a sufficient quantity. Thus, the grown vitroplants on the medium (MS/2+BAP+COU) presented the best values which are related to the percentage of tuberization, the number of microtubercles / vitroplants and weight of tubers. Moreover, the Bartina genotype showed a remarkable superiority over its media and under a 16h/8h photoperiod except for the diameter of the tubers, where this genotype had the best diameter over the medium (MS/2+KIN) under an 8h photoperiod.

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