scholarly journals The spectrum of molecules of medium mass as endogenous intoxication indicator in quails with mycotoxicosis

2020 ◽  
Vol 210 ◽  
pp. 06010
Author(s):  
Elena Kuzminova ◽  
Evgeny Dolgov ◽  
Marina Semenenko ◽  
Denis Osepchuk ◽  
Ksenia Semenenko
Keyword(s):  
Blood Serum ◽  
Aflatoxin B1 ◽  
Pathological Process ◽  
Maximum Permissible Level ◽  
Permissible Level ◽  
Endogenous Intoxication ◽  
Medium Mass ◽  
Different Types ◽  
Distribution Index

The paper presents the results of determining the level of molecules of medium mass (MMM) in the blood serum of poultry with experimental mycotoxicosis. The study revealed a relationship between the concentration of MMM in the blood serum and the severity of the pathological process in poultry with different types of mycotoxicosis. The most pronounced changes occur in mycotoxicosis due to the combined intake of T-2 toxin and aflatoxin B1 into the poultry body, even when their concentration individually does not exceed the maximum permissible level. In this case, a significant increase in the concentration of MMM in the blood serum is recorded – in 1.8 times (λ = 254 nm) and in 2.2 times (λ = 280 nm), with an increase in the MMM distribution index. Next, according to the severity of endogenous intoxication, follows mycotoxicosis caused by aflatoxin B1, and the lowest values were recorded with T-2 toxicosis.

scholarly journals Antioxidant-rela ted blood prooxidative patients with acute respiratory symptoms of children with the disease in the mouth

2015 ◽  
Vol 17 (4) ◽  
Author(s):  
N. O. Hevkalyuk
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Iron Content ◽  
Respiratory Symptoms ◽  
Pathological Process ◽  
Endogenous Intoxication

<p>There was set the intensification of lipid peroxidation, as evidenced by the increasing content of DC and MDA<br />in serum of patients with ARV I children on the background discoordination functioning of the AOC , which manifests<br />itself in an increase in activity of ceruloplasmin and the decrease in saturation of transferrin and iron content of<br />catalase in blood serum, the growth of endogenous intoxication, the severity of which depends on the severity of<br />the pathological process.</p>

scholarly journals Interrelation between endogenous intoxication indicators and nitric oxide metabolites in the infectious process in chronic viral hepatitis C

2016 ◽  
Vol 97 (4) ◽  
pp. 540-544
Author(s):  
N V Galeeva ◽  
V Kh Fazylov ◽  
I Kh Valeeva
Keyword(s):  
Nitric Oxide ◽  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Blood Serum ◽  
Nitrogen Oxide ◽  
Antiviral Therapy ◽  
Chronic Hepatitis C ◽  
Endogenous Intoxication ◽  
Medium Mass ◽  
Nitric Oxide Metabolites

Aim. To study dynamics and interrelation between final nitric oxide metabolites (nitrites) and endogenous intoxication index (medium mass molecules) in patients with chronic hepatitis C with the effect of antiviral therapy on them.Methods. The study included 99 patients with chronic hepatitis C. The combination therapy for 48 weeks in patients with genotype 1 chronic hepatitis C with pegylated interferon (peginterferon alfa-2a and alfa-2b) in combination with ribavirin and for 24 weeks with daily standard interferon alfa-2b administration in patients with virus genotypes 2 and 3 was conducted. The nitric oxide level was estimated by the content of the nearest metabolite (nitrite) in blood serum using the P.P. Golikov method. Medium mass molecules in the blood serum were determined by the N.I. Gabrielyan method.Results.In patients with chronic hepatitis C high values of nitrogen oxide metabolites and the medium mass molecules in the blood serum were revealed, whereas the level of nitroxidemia and endogenous intoxication by the values of medium mass molecules was associated with the viral replicative capacity value and alanine aminotransferase serum levels. Amid the combination antiviral therapy the nature of nitroxidergy changed with the normalization of nitrogen oxide indicators by 24 weeks and an increase to 48 weeks of treatment. There were significantly positive correlative relations between pronouncement of levels of nitric oxide metabolites and the medium mass molecules before the start of antiviral therapy (R=0.292629, pConclusion. Chronic hepatitis C promotes increase in nitrite ion content and medium mass molecules in the blood serum, their level is associated with virus replication activity and cytolysis severity; antiviral therapy has an effect on the level of endogenous intoxication with the normalization of content of the nitrite ions and medium mass molecules in blood serum by 24th week of treatment.

METHODS FOR SIMULATION OF ACUTE BRAIN ISCHEMIA: PATHOPHYSIOLOGICAL SUBSTANTIATION OF SELECTION AND IMPORTANCE FOR CLINICAL PRACTICE

2019 ◽  
pp. 142-152
Author(s):  
Б.И. Гельцер ◽  
Э.В. Слабенко ◽  
Ю.В. Заяц ◽  
В.Н. Котельников
Keyword(s):  
Clinical Practice ◽  
Experimental Studies ◽  
Cerebrovascular Diseases ◽  
Experimental Models ◽  
Pathological Process ◽  
Ischemic Brain ◽  
Actual Clinical Practice ◽  
Acute Cerebral Ischemia ◽  
Different Types ◽  
Acute Brain Ischemia

Одним из основных требований к разработке экспериментальных моделей цереброваскулярных заболеваний является их максимальная приближенность к реальной клинической практике. В работе систематизированы данные по основным методам моделирования острой ишемии головного мозга (ОИГМ), представлена их классификация, анализируются данные о преимуществах и недостатках той или иной модели. Обсуждаются результаты экспериментальных исследований по изучению патогенеза ОИГМ с использованием различных моделей (полной и неполной глобальной, локальной и мультифокальной ишемии) и способов их реализации (перевязка артерий, клипирование, коагуляция, эмболизация и др.). Особое внимание уделяется «стабильности» последствий острого нарушения мозгового кровообращения: необратимых ишемических повреждений головного мозга или обратимых с реперфузией заданной продолжительности. Отмечается, что важное значение в этих исследованиях должно принадлежать современным методам прижизненной визуализации очагов острого ишемического повреждения, что позволяет оценивать динамику патологического процесса. Предлагаемый метод отвечает требованиям гуманного обращения с животными. Подчеркивается, что выбор релевантной модели ОИГМ определяется задачами предстоящего исследования и технологическими ресурсами научной лаборатории. Development of experimental models for acute forms of cerebrovascular diseases is essential for implementation of methods for their prevention and treatment. One of the principal requirements to such models is their maximum approximation to actual clinical practice. This review systematized major models of acute cerebral ischemia (ACI), their classification, and presented information about their advantages and shortcomings. Also, the review presented results of experimental studies on pathophysiological mechanisms of different types of modeled ACI (complete and incomplete global, local, and multifocal ischemia) and methods for creating these models (arterial ligation, clipping, coagulation, embolization, etc.). Particular attention was paid to “stability” of the consequences of acutely impaired cerebral circulation - an irreversible ischemic brain injury or a reversible injury with reperfusion of a given duration. The authors emphasized that in such studies, a special significance should be given to intravital imaging of acute ischemic damage foci using modern methods, which allow assessing the dynamics of the pathological process and meet the requirements to humane treatment of animals. The choice of a relevant ACI model is determined by objectives of the planned study and the technological resources available at the research laboratory.

scholarly journals Evaluation of the nitrate and nitrite content of vegetables commonly grown in Slovenia

2017 ◽  
Vol 12 (2) ◽  
Author(s):  
Veronika Kmecl ◽  
Tea Knap ◽  
Dragan Žnidarčič
Keyword(s):  
European Union ◽  
Human Body ◽  
Daily Intake ◽  
Agricultural Area ◽  
Segmented Flow ◽  
Maximum Permissible Level ◽  
Eu Legislation ◽  
Permissible Level ◽  
Nitrate And Nitrite ◽  
Nitrite Content

Nitrate (NO<sub>3</sub><sup>–</sup>) and nitrite (NO<sub>2</sub><sup>–</sup>) levels of a total 1195 samples of nine different vegetables (lettuce, potato, cabbage, carrot, string beans, tomato, cucumber, cauliflower and pepper) collected at several locations of an intensive agricultural area in Slovenia were analysed during a period of 13 years. The content of NO<sub>2</sub><sup>–</sup> and NO<sub>3</sub><sup>–</sup> ions in commercial mature samples was determined using a segmented flow analyser. The average NO<sub>3</sub><sup>–</sup> content was the highest in lettuce (962 mg/kg), cabbage (795 mg/kg), string beans (298 mg/kg), carrot (264 mg/kg), cauliflower (231 mg/kg), potato (169 mg/kg) and was moderately high in cucumber (93 mg/kg) and pepper (69 mg/kg). A low NO<sub>3</sub><sup>–</sup> content was found in tomato (2<sup>–</sup> did not exceed 0.5 mg/kg, with the exception of potato (1.08 mg/kg). Six samples of lettuce exceeded the maximum permissible level of NO<sub>3</sub><sup>–</sup> according to current European Union (EU) legislation. Based on the results of our investigation, we assessed the approximate daily intake (DI) of NO<sub>3</sub>– and NO<sub>2</sub><sup>–</sup> to human body. The results indicated that with the consumption of potato, the daily intake per inhabitant is close to the acceptable DI permitted in EU.

scholarly journals Effect of different types of stress on adrenal gland parameters and adrenal hormones in the blood serum of male Wistar rats

2009 ◽  
Vol 61 (2) ◽  
pp. 187-194 ◽  
Author(s):  
M. Adzic ◽  
Ana Djordjevic ◽  
Jelena Djordjevic ◽  
Ana Niciforovic ◽  
Marija Radojcic
Keyword(s):  
Blood Serum ◽  
Wistar Rats ◽  
Adrenal Glands ◽  
Acute Stress ◽  
Serum Levels ◽  
Combined Stress ◽  
Adrenal Hormones ◽  
General Adaptation ◽  
Different Types ◽  
Male Wistar Rats

In the present study, we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex and medulla in mature male Wistar rats subjected to three different stress types: acute, chronic, and combined, i.e., chronic followed by acute stress. These parameters were correlated with adrenal activity as judged from serum levels of corticosterone and catecholamine, respectively, as well as with serum levels of ACTH and glucose. Under all three conditions, we observed bilaterally asymmetric and stress-type-independent hypertrophy of whole adrenals, as well as adrenal cortices and medullas. Under acute and combined stress, adrenal hypertrophy was followed by increase of adrenal hormones in the blood serum. However, under chronic stress, both cortical and medullar activities as judged from low or unaltered levels of the respective hormones and glucose were compromised and disconnected from the input signal of ACTH. Since all of the studied adrenal activities could be restored by subsequent acute stress, it is concluded that chronic isolation can be viewed as partly maladaptive stress with characteristics resembling stress resistance rather than the stress exhaustion stage of the general adaptation syndrome.

scholarly journals EXTRAPLEURAL PNEUMOLYSIS FOR CAVITARY PULMONARY TUBERCULOSIS TREATMENT

2020 ◽  
pp. 15-20
Author(s):  
P. G. Gadzhieva ◽  
D. B. Giller ◽  
A. A. Glotov ◽  
O. Sh. Kesaev ◽  
V. V. Koroev ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Multidrug Resistant ◽  
Pathological Process ◽  
Tuberculosis Treatment ◽  
Long Term Results ◽  
Filling Material ◽  
Different Types ◽  
Positive Effect ◽  
Observation Period

Objective. To increase efficiency of cavitary pulmonary tuberculosis treatment by developing modern indications and technology of extrapleural pneumolysis with filling material.Material and methods. We reviewed 25 patients with cavitary pulmonary tuberculosis and bacterial excretion who had undergone extrapleural pneumolysis with different types of filling material in 2004–2015. In 16 cases, we performed surgery in patients with one lung.Results. 56% of operated patients demonstrated extensively drug-resistant TB, 40% had multidrug-resistant TB. Cavities were closed and bacterial excretion ceased in 92% of patients at discharge. Long-term results with observation period from 1 to 12 years were analyzed in 24 patients, 17 (71%) of them had complete clinical efficacy.Conclusion. Extrapleural pneumolysis is a mini-invasive surgical treatment that can prove positive effect in patients with non-fibrotic changes in a cavity wall and pathological process in upper parts of the lungs. 

scholarly journals Зміни в вітамінній ланці антиоксидантної системи корів різних типів вищої нервової діяльності

2017 ◽  
Vol 19 (78) ◽  
pp. 81-85
Author(s):  
Yu.O. Sysyuk ◽  
V.I. Karpovskiy ◽  
O.V. Zhurenko ◽  
O.V. Danchuk ◽  
R.V. Postoy
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Blood Serum ◽  
Significant Influence ◽  
Weak Type ◽  
Nervous Activity ◽  
Winter Season ◽  
Tocopherol Content ◽  
Higher Nervous Activity ◽  
Different Types

The article presents the results of the studying the content of some vitamins in blood serum of cows with different types of higher nervous activity depending on the season. The purpose of the study was to determine the influence of the typological features of the nervous system on the content of separate vitamins in blood serum of cows depending on the season. The experiment was carried out on the basis of the farm «Kolos» village Borodianka, Kyiv region on clinically healthy cows of Ukrainian black-and-white dairy breed of 2–3rd lactation. On the basis of the investigation the conditioned reflex activity, 4 experimental groups of animals were formed with 4 most typical representatives of the identified types of higher nervous activity in each: 1st group – strong balanced mobile type, 2nd group – strong balanced inert type, 3rd group – strong unbalanced type, 4th group – weak type. The research material was blood serum, in which the content of vitamins A, E and C was determined by express method via liquid chromatography. The conducted investigation of ascorbic acid, retinol and tocopherol content in blood serum of cows with different types of higher nervous activity in winter and summer periods shown that its content is significantly dependent on the season. In particular, the content of ascorbic acid in blood serum of cows with different types of higher nervous activity in the winter season was lower by 22–27% in compare with its meaning in the summer season. It should be noted that the largest difference in content of vitamin C in blood serum depending on the season was found in animals of strong balanced inert and weak types of higher nervous activity (by 27.4%, P < 0.001 and 24.0%, P < 0.001 respectively). While in animals of strong balanced mobile and strong unbalanced types of higher nervous activity the content of vitamin C in blood serum was lower by 23.2% (P < 0.001) and 21.7% (P < 0.001), respectively. The content of fat-soluble vitamins in blood serum of cows with different types of higher nervous activity is also largely dependent on the season. Thus, the content of tocopherol was lower in the winter season by 35.8–41.6% (P < 0.001) depending on the type of higher nervous activity. And the retinol content was lower by 49.2–55.3% (P FU=3.0; P FU = 3.0; P < 0.05. However, the main properties of cortical processes did not have a significant influence on the content of retinol in blood serum – F = 2.8 < FU = 3.0; P = 0.06. The season has a significant influence on the content of the above mentioned vitamins – F = 126–348 > FU = 4.3; P < 0.001, which is obviously due to the level of supply the animal’s body with vitamins during different seasons. It should be noted that there is no relationship between the sources of variation (type of higher nervous activity and season), which obviously testifies to the absence of influence of the seasons on the main features of cortical processes (F = 0.37–1.64 < FU = 3.0; P = 0.208–0.775). Thus, the influence of the main features of cortical processes on the content of ascorbic acid, retinol and tocopherol in blood serum of cows is found. In animals of weak type of higher nervous activity the vitamin content in blood serum is lower than in animals of strong types. The lower content of ascorbic acid, retinol and tocopherol in blood serum of cows in the winter season, regardless the type of higher nervous activity, has been established.

scholarly journals WAYS TO IMPROVE THE RESULTS OF TREATMENT OF PATIENTS WITH CHOLELITHIASIS AND MECHANICAL JAUNDICE.

ASJ. ◽  
2021 ◽  
Vol 1 (56) ◽  
pp. 33-39
Author(s):  
R. Kalibatov ◽  
O. Logvina ◽  
R. Kalmykova
Keyword(s):  
Bile Ducts ◽  
Surgical Intervention ◽  
Pathological Process ◽  
Control Group ◽  
Results Of Treatment ◽  
Different Types ◽  
Complications Of Cholelithiasis ◽  
Personalized Approach ◽  
Complications And Mortality ◽  
Medium Weight

Purpose. To create a personalized tactic of surgical treatment of patients with BSD and MJ considering the stage of the pathological process and thereby improve the results of treatment. Materials and methods. The experience of diagnosis and treatment of 537 patients with complications of cholelithiasis, manifested by mechanical jaundice, was analyzed. The first stage was 537 minimally invasive decompressions of the biliary tract, the second-500 different types of endosurgical interventions. Results. Depending on the tactical principles, all the subjects were divided into two groups. In one (control) group (n = 249), patients were admitted from 2010 to 2014. Their treatment was empirical: decompression of the bile tree was performed at different times, and surgery was performed without taking into account the stages of the pathological process and the severity of the patients. In the other analyzed group (n = 251), patients were admitted to the hospital from 2015 to 2019. Their treatment tactics were personalized: taking into account the stages of mechanical jaundice. individual terms of decompression of the bile ducts and the choice of surgical intervention were determined. When comparing the results obtained, the advantage of the personalized approach was established: a decrease in the percentage of postoperative complications and mortality was revealed. Conclusion. In patients with cholelithiasis and jaundice, the severity of the condition is due to hyperbilirubinemia. Stage 2 of mechanical jaundice-determined by the severity of cytolysis in hepatic insufficiency, manifested by hypertransaminasemia. The severity of cholangitis is determined by purulent-inflammatory, as well as septic signs of the disease, characterized by leukocytosis, circulating immune complexes and medium-weight molecules. In the cholestatic stage of jaundice development, treatment is planned, in the cytolytic stage — urgent, and in cholangitis-emergency. The use of personalized tactics for the treatment of patients with breast cancer has reduced the number of complications and mortality.

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