scholarly journals Effects of reciprocal hybridization on cadmium accumulation in F1 hybrids of Solanum diphyllum and Solanum nigrum

2020 ◽  
Vol 218 ◽  
pp. 03006
Author(s):  
Renyan Liao ◽  
LingYu Ma
Keyword(s):  
Cadmium Accumulation ◽  
Solanum Nigrum ◽  
Pot Experiment ◽  
F1 Hybrids ◽  
F1 Hybrid ◽  
Cd Accumulation ◽  
Significant Difference ◽  
Accumulation Ability ◽  
Soil Cd

A pot experiment was conducted to study the effects of reciprocal hybridization on cadmium (Cd) accumulation in F1 hybrid of Solanum diphyllum and Solanum nigrum by Cd treatment (the final soil Cd concentration was 10 mg/kg). The results showed that the Cd content of F1 hybrid was between the parents. In stems, leaves and shoots, the Cd content of DN (S. diphyllum male × S. nigrum female) and ND (S. nigrum male × S. diphyllum female) F1 hybrid increased by 14.09% and 17.34%, 8.08% and 11.14%, 9.5% and 12.04% respectively compared with S. nigrum, and decreased by 13.34% and 10.87%, 6.52% and 3.88%, 7.44% and 5.0% respectively compared with S. diphyllum, with no significant difference in roots. Therefore, the accumulation ability of Cd in F1 hybrids was improved to a certain extent compared to S. nigrum, but decreased to a certain extent compared to S. diphyllum.

scholarly journals Adsorption of Cadmium by Brassica juncea (L.) Czern. and Brassica pekinensis (Lour.) Rupr in Pot Experiment

2021 ◽  
Vol 14 (1) ◽  
pp. 429
Author(s):  
Rongrong Ying ◽  
Bing Xia ◽  
Xiaowen Zeng ◽  
Rongliang Qiu ◽  
Yetao Tang ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Contaminated Soil ◽  
Human Life ◽  
Translocation Factor ◽  
Cadmium Accumulation ◽  
Pot Experiment ◽  
Cd Accumulation ◽  
Biomass Reduction ◽  
Brassica Pekinensis ◽  
Soil Cd

Heavy metal pollution in farmland threatens human life. It is not clear whether crops can adsorb heavy metals. In this study, the cadmium accumulation and tolerance in Chinese cabbage Brassica pekinensis (cv. Xiaoza-56) and the known Cd-accumulator Brassica juncea in hydroponics and pot experiment were investigated. Furthermore, we evaluated their potential on the phytoextraction of Cd-contaminated soil. The hydroponics with 1–50 μM Cd concentrations showed that both B. juncea and B. pekinensis had high Cd accumulation and tolerance with translocation factor closed to 1 at Cd levels < 25 μM. The pot study conducted with 5 to 100 mg Cd kg−1 soil indicated that B. juncea showed less tolerance and accumulation to Cd than B. pekinensis, especially at higher Cd levels. The bioconcentration factor was much higher than 1 in both B. juncea and B. pekinensis grown in <40 mg Cd kg−1 soil without showing biomass reduction. In the model evaluation, the ability of B. juncea and B. pekinensis to reduce the initial soil Cd concentration of 20 and 5 mg kg−1 to specific targets with a lower or higher biomass of 4 or 20 t ha−1, respectively. The above results indicate that B. juncea and B. pekinensis (cv. Xiaoza-56), which the latter is a better candidate for Cd phytoextraction in moderated Cd-contaminated soil. The results provide a reference for Cd pollution control.

scholarly journals Enhanced Cadmium Accumulation and Tolerance in Transgenic Hairy Roots of Solanum nigrum L. Expressing Iron-Regulated Transporter Gene IRT1

Life ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 324
Author(s):  
Peng Ye ◽  
Menghua Wang ◽  
Teng Zhang ◽  
Xiaoyu Liu ◽  
He Jiang ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Cadmium Accumulation ◽  
Solanum Nigrum ◽  
Antioxidant Enzyme Activities ◽  
Transporter Gene ◽  
Cd Stress ◽  
Cd Accumulation ◽  
Transgenic Hairy Roots ◽  
Solanum Nigrum L ◽  
Iron Regulated Transporter

Solanum nigrum L., a hyperaccumulator of cadmium (Cd), is regarded as a promising candidate for phytoremediation of heavy metal pollution. In the present study, the hairy roots of Solanum nigrum L. were selected as a model plant system to study the potential application of Iron-regulated Transporter Gene (IRT1) for the efficient phytoremediation of Cd pollution. The transgenic hairy roots of Solanum nigrum L. expressing the IRT1 gene from Arabidopsis thaliana were successfully obtained via the Agrobacterium tumegaciens-mediated method. Expression of IRT1 reduced Cd stress-induced phytotoxic effects. Significantly superior root growth, increased antioxidant enzyme activities, decreased reactive oxygen species (ROS) levels, and less cell apoptosis were observed in the transgenic hairy roots of Solanum nigrum L. compared to the wild-type lines under Cd stress. Enhanced Cd accumulation was also carried out in the transgenic hairy roots compared to the control (886.8 μg/g vs. 745.0 μg/g). These results provide an important understanding of the Cd tolerance mechanism of transgenic IRT1 hairy roots of Solanum nigrum L., and are of particular importance to the development of a transgenic candidate for efficient phytoremediation process.

scholarly journals The Addition of KNO3 and (NH4)3PO4 Maybe Improves the Ability to Accumulate Cd by Regulating the Redistribution of Nutrients in Bermudagrass

2021 ◽  
Author(s):  
Shuduan Tan ◽  
Bin Chen ◽  
Zhongshu Liu ◽  
Meng Dong ◽  
Hongbing Yu ◽  
...  
Keyword(s):  
Dry Weight ◽  
Water Soluble ◽  
Available N ◽  
Cd Accumulation ◽  
Chlorophyll Contents ◽  
Hydroponic Experiment ◽  
Significant Difference ◽  
Potential Activity ◽  
Enrichment Efficiency ◽  
Accumulation Ability

Abstract The application of fertilizer can improve the Cd enrichment efficiency of plants, but the changes of nitrogen (N), phosphorus (P), potassium (K) in the growth medium have different impacts on Cd enrichment efficiency of plants. In this study, hydroponic experiment by adding different KNO3 and (NH4)3PO4 was carried out to study whether and how the addition of available N, P and K can improve Cd accumulation. The four treatments were CK, 0.4 mg kg-1 Cd (Cd), 0.4 mg kg-1 Cd and KNO3 (COK), 0.4 mg kg-1 Cd and (NH4)3PO4 (CNP). The results showed that the chlorophyll contents, PSII maximum photochemical quantum efficiency (Fv/Fm) and PSII potential activity (Fv/Fo) of COK and CNP were higher than that of CD. There was no significant difference between CK and CNP (P > 0.05). The N, P and K contents and Cd concentrations of roots and stems of COK and CNP were significantly higher than those of CD(P＜0.05).The BCF of COK and CNP were also significantly higher than that of CD, increasing by 39.8% and 37.6%, respectively. The root, stem and leaf dry weight of COK and the root and stem dry weight of CNP were higher than that of CD, and the biomass of the whole basin was significantly higher than that of CD (P＜0.05), but with no significant difference from that of CK (P＞0.05). In addition to stem K accumulation of CNP, N, P and K allocated to root and stem in COK and CNP were significantly higher than that in CD (P＜0.05). There was no significant difference in pH and water soluble Cd content among CD, COK and CNP (P＞0.05). it was concluded that plants can improve their Cd accumulation ability by changing biomass and nutrient allocation. The conclusion provides a theoretical basis for improving soil environmental factors by improving fertilization strategies of related plants so as to improve Cd enrichment ability.

scholarly journals Study on cadmium accumulation characteristics of Solanum nigrum with different ploidies

2019 ◽  
Vol 136 ◽  
pp. 07012
Author(s):  
Yunmin Huan ◽  
Zhouyang Jiu ◽  
Huixuan Zhou ◽  
Haoran Zhang ◽  
Yong Huang ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Translocation Factor ◽  
Cadmium Accumulation ◽  
Solanum Nigrum ◽  
Pot Experiment ◽  
Accumulation Factor ◽  
Cadmium Content ◽  
Accumulation Characteristics ◽  
Cadmium Contaminated Soil

To study the cadmium accumulation characteristics of Solanum nigrum with different ploidies, the biomass, cadmium content and cadmium accumulation of diploid (Solanum photeinocarpum), tetraploid (Solanum photeinocarpum) and hexaploid (Solanum nigrum) were measured by a pot experiment. The results showed that the biomass of roots, stems, leaves and shoots of S. nigrum plants with different ploidy all ranked as follows: hexaploid > tetraploid > diploid. Cadmium content and cadmium accumulation in stems, leaves and shoots of hexaploid plants were significantly higher than those in tetraploid and diploid plants, and the cadmium translocation factor and translocation accumulation factor of hexaploid were the largest, too. Therefore, hexaploid S. nigrum had a highest ability to extract and transport cadmium, which can be used as an ideal plant for remediation of cadmium-contaminated soil.

scholarly journals Effects of hyperaccumulator plant straw on biomass and cadmium accumulation of lettuce

2019 ◽  
Vol 136 ◽  
pp. 07005
Author(s):  
Le Liang ◽  
Ran Zhang ◽  
Yan Zhao ◽  
Ying Zhu ◽  
Qiaoman Ao ◽  
...  
Keyword(s):  
Cadmium Accumulation ◽  
Solanum Nigrum ◽  
Cd Stress ◽  
Bidens Pilosa ◽  
Cd Uptake ◽  
Cd Accumulation ◽  
Accumulator Plant ◽  
Hyperaccumulator Plant ◽  
Straw Application ◽  
Ground Part

To study the effects of hyper-accumulator plant straw on the biomass and cadmium (Cd) accumulation of lettuce (Lactuca sativa), the pot experiments were conducted to study the effects of straw application of three hyper-accumulator plants (Solanum nigrum, Bidens pilosa and Galinsoga parviflora) on the biomass and Cd accumulation of lettuce under Cd stress. The results show that: compared with no straw application, the biomass of lettuce was increased after applying three kinds of hyper-accumulator plant straw: S. nigrum, B. pilosa and G. parviflora, Cd content in the above ground part of lettuce was increased by 4.46%, 1.20% and 0.63% respectively, compared with that of non-application, and Cd content in the root of lettuce was decreased. The application of three kinds of hyperaccumulator plant straw promoted the growth of lettuce and increased Cd uptake by lettuce of aerial part.

Joint Effect of Cadmium and Chlorimuron-Ethyl on Cadmium Accumulation of Wheat

2012 ◽  
Vol 518-523 ◽  
pp. 426-429
Author(s):  
Cai Xia Jin ◽  
Qi Xing Zhou ◽  
Jing Fan ◽  
Xue Ying Song
Keyword(s):  
Negative Relationship ◽  
Cadmium Accumulation ◽  
Joint Effect ◽  
Antagonistic Effect ◽  
Mature Stage ◽  
Cd Accumulation ◽  
Wheat Shoot ◽  
Chlorimuron Ethyl ◽  
Wheat Tissues ◽  
Soil Cd

The joint effects of Cd and Chlorimuron-ethyl(CE) on Cd accumulation in wheat shoot/root and SPT coefficient of Cd was carried out. Cd concentration in shoot and root increased with increasing soil Cd, but Cd concentration in shoot/root was lower than the same treatment when CE was added, and negative relationship between Cd concentration in shoot (root) and concentration of CE were significant (p<0.05 or p<0.01). There was antagonistic effect of Cd and low CE on Cd accumulation in wheat tissues. Cd concentration in shoot decreased during the period of growth, but the concentration of Cd in root increased from the elongation stage to the florescence, and then decreased from the florescence to mature stage when the concentration of soil Cd was less than 100 mg kg-1, and it decreased when the concentration of soil Cd was 100 mg kg-1. The SPT values of different growth periods decreased during the period of growth.

scholarly journals Sequencing an F1 hybrid of Silurus asotus and S. meridionalis enabled the assembly of high-quality parental genomes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Weitao Chen ◽  
Ming Zou ◽  
Yuefei Li ◽  
Shuli Zhu ◽  
Xinhui Li ◽  
...  
Keyword(s):  
De Novo ◽  
Parental Species ◽  
F1 Hybrids ◽  
Pelteobagrus Fulvidraco ◽  
F1 Hybrid ◽  
Short Reads ◽  
Final Assembly ◽  
Genome Complexity ◽  
Hybrid Genome ◽  
Silurus Asotus

AbstractGenome complexity such as heterozygosity may heavily influence its de novo assembly. Sequencing somatic cells of the F1 hybrids harboring two sets of genetic materials from both of the paternal and maternal species may avoid alleles discrimination during assembly. However, the feasibility of this strategy needs further assessments. We sequenced and assembled the genome of an F1 hybrid between Silurus asotus and S. meridionalis using the SequelII platform and Hi-C scaffolding technologies. More than 300 Gb raw data were generated, and the final assembly obtained 2344 scaffolds composed of 3017 contigs. The N50 length of scaffolds and contigs was 28.55 Mb and 7.49 Mb, respectively. Based on the mapping results of short reads generated for the paternal and maternal species, each of the 29 chromosomes originating from S. asotus and S. meridionalis was recognized. We recovered nearly 94% and 96% of the total length of S. asotus and S. meridionalis. BUSCO assessments and mapping analyses suggested that both genomes had high completeness and accuracy. Further analyses demonstrated the high collinearity between S. asotus, S. meridionalis, and the related Pelteobagrus fulvidraco. Comparison of the two genomes with that assembled only using the short reads from non-hybrid parental species detected a small portion of sequences that may be incorrectly assigned to the different species. We supposed that at least part of these situations may have resulted from mitotic recombination. The strategy of sequencing the F1 hybrid genome can recover the vast majority of the parental genomes and may improve the assembly of complex genomes.

scholarly journals Fitness of F1 hybrids between 10 maternal wild soybean populations and transgenic soybean

2021 ◽  
Author(s):  
Jin Yue Liu ◽  
Ze Wen Sheng ◽  
Yu Qi Hu ◽  
Qi Liu ◽  
Sheng Qiang ◽  
...  
Keyword(s):  
Seed Weight ◽  
Wild Soybean ◽  
F1 Hybrids ◽  
Weed Competition ◽  
Transgenic Soybean ◽  
Wild Progenitor ◽  
F1 Hybrid ◽  
Dry Biomass ◽  
100 Seed Weight ◽  
Pod Number

AbstractThe releasing of transgenic soybeans (Glycine max (L.) Merr.) into farming systems raises concerns that transgenes might escape from the soybeans via pollen into their endemic wild relatives, the wild soybean (Glycine soja Sieb. et Zucc.). The fitness of F1 hybrids obtained from 10 wild soybean populations collected from China and transgenic glyphosate-resistant soybean was measured without weed competition, as well as one JLBC-1 F1 hybrid under weed competition. All crossed seeds emerged at a lower rate from 13.33–63.33%. Compared with those of their wild progenitors, most F1 hybrids were shorter, smaller, and with decreased aboveground dry biomass, pod number, and 100-seed weight. All F1 hybrids had lower pollen viability and filled seeds per plant. Finally, the composite fitness of nine F1 hybrids was significantly lower. One exceptional F1 hybrid was IMBT F1, in which the composite fitness was 1.28, which was similar to that of its wild progenitor due to the similarities in pod number, increased aboveground dry biomass, and 100-seed weight. Under weed competition, plant height, aboveground dry biomass, pod number per plant, filled seed number per plant, and 100-seed weight of JLBC-1 F1 were lower than those of the wild progenitor JLBC-1. JLBC-1 F1 hybrids produced 60 filled seeds per plant. Therefore, F1 hybrids could emerge and produce offspring. Thus, effective measures should be taken to prevent gene flow from transgenic soybean to wild soybean to avoid the production F1 hybrids when releasing transgenic soybean in fields in the future.

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