final assembly
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GigaScience ◽  
2022 ◽  
Vol 11 (1) ◽  
Author(s):  
Olli-Pekka Smolander ◽  
Daniel Blande ◽  
Virpi Ahola ◽  
Pasi Rastas ◽  
Jaakko Tanskanen ◽  
...  

Abstract Background The Glanville fritillary (Melitaea cinxia) butterfly is a model system for metapopulation dynamics research in fragmented landscapes. Here, we provide a chromosome-level assembly of the butterfly's genome produced from Pacific Biosciences sequencing of a pool of males, combined with a linkage map from population crosses. Results The final assembly size of 484 Mb is an increase of 94 Mb on the previously published genome. Estimation of the completeness of the genome with BUSCO indicates that the genome contains 92–94% of the BUSCO genes in complete and single copies. We predicted 14,810 genes using the MAKER pipeline and manually curated 1,232 of these gene models. Conclusions The genome and its annotated gene models are a valuable resource for future comparative genomics, molecular biology, transcriptome, and genetics studies on this species.


2022 ◽  
pp. 72-106
Author(s):  
Erick McGregor Roa-Badilla ◽  
Gabriela Jacobo-Galicia ◽  
Mildrend Ivett Montoya-Reyes ◽  
Ismael Mendoza-Muñoz ◽  
Juan Ceballos- Corral

Workers are exposed to different factors that can be detrimental to their well-being, being the most known safety and hygiene factors. A few years ago, the concepts of harmful factors for workers were updated by adding the psychosocial factors. Although there is literature on psychosocial factors and their effect on health, this work focused specifically on seeking the relationship between productivity and psychosocial factors to know how it impacts the organization. The investigation was applied in a case study for a doorknob locks manufacturing company in its final assembly area. Data collection was done by applying the questionnaire provided by NOM-035-STPS-2018 and the company's productivity databases for the subsequent analysis using statistical tools. The results were that, for the violence factor, its correlation with productivity was R2 = 0.8886, indicating a strong correlation with which it is concluded that there is an influence on productivity.


Author(s):  
Adolfo Cano-Carrasco ◽  
René Daniel Fornés-Rivera ◽  
María Del Carmen Vásquez-Torres ◽  
Arlene Amalia Guerrero-Portillo

This research addresses the problem of leveling workloads in a multi-product final assembly area. In which it was found that 27.4% of the time is used for set up and the current distribution presents areas of opportunity. The target was to implement improvement actions to make use of resources more efficient in the production process in the aforementioned area through Lean Manufacturing tools. The results obtained consist of eight products generated with the support of lean manufacturing support tools such as SMED, Workload Balancing and MUDA waste identification, achieving important results among which productivity in the area stands out from 109% to 125%, as well as a reduction in set-up time from 17 min to 4.4 min.


Author(s):  
Armagan Altinisik ◽  
Emre Bolova

The prediction model for the energy contributors is studied to define the effect of door components. The model is validated by experimental measurements on produced vehicles. The correlation of the control results and the evaluation methods among the representative customers, appraisers and senior experts are performed and compared by objective energy measurements. The subjective evaluation aims to clarify real customer expectations in door closing process and carry it to new product development phase. This will also improve the competency of appraisers in final assembly approval zones. The energy contributors for boot swing door is numerically calculated and validated by experimental measurements in easy and hard-closing doors. Subjective evaluations in 45 vehicles with different closing energies were performed by three different groups as representative customers, appraisers, and senior experts. The results were evaluated by statistical tools. The major energy contributors were found as air binding, seal resistance and check strap both in the calculations and in the experiments. The results demonstrated good correlation between numerical model and objective measurements. Besides, the subjective evaluations showed parallel trends with objective energy measurements. The variation in customer evaluations is more than appraisers and experts. Customers are severer in the evaluations of hard-closing doors and more sensitive in 10° door closing angle. This is a unique study for rear boot swing door closing effort in an LCV model. The study contains both definitions of factors, components that contributes in door closing energy and subjective evaluations to correlate the closing behaviors of representative customers, appraisers, and senior experts. The output of the study would be used to improve the vehicle control plan (VCP) and design standards in new model development phase.


Viruses ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 15
Author(s):  
Veronica Sanchez ◽  
William Britt

The assembly of human cytomegalovirus (HCMV) and other herpesviruses includes both nuclear and cytoplasmic phases. During the prolonged replication cycle of HCMV, the cell undergoes remarkable changes in cellular architecture that include marked increases in nuclear size and structure as well as the reorganization of membranes in cytoplasm. Similarly, significant changes occur in cellular metabolism, protein trafficking, and cellular homeostatic functions. These cellular modifications are considered integral in the efficient assembly of infectious progeny in productively infected cells. Nuclear egress of HCMV nucleocapsids is thought to follow a pathway similar to that proposed for other members of the herpesvirus family. During this process, viral nucleocapsids must overcome structural barriers in the nucleus that limit transit and, ultimately, their delivery to the cytoplasm for final assembly of progeny virions. HCMV, similar to other herpesviruses, encodes viral functions that co-opt cellular functions to overcome these barriers and to bridge the bilaminar nuclear membrane. In this brief review, we will highlight some of the mechanisms that define our current understanding of HCMV egress, relying heavily on the current understanding of egress of the more well-studied α-herpesviruses, HSV-1 and PRV.


2021 ◽  
Author(s):  
Doni Riadi ◽  
◽  
Ade Geovania Azwar ◽  

Base Single Part is the main component of the cable installation that functions as a connector between cables in the aircraft. As a critical part, the Base Single Part is the most important sub-component that must be completed to be installed in the aircraft part. Base Single Part is a part that is used in all types of aircraft which are specially produced at PT. Dirgantara Indonesia, in one aircraft this component can be installed in the amount of 8000-9000 Base Single Part. In relation to the request from the Final Assembly Line section regarding the request for the Base Single Part component, there are still some products that do not meet the specifications so that they have to be repaired, such as holes that do not match the size, this causes the delivery order process to the Final Assembly Line section to be hampered and disruptive smooth production. The purpose of this research is to control the quality of the Base Single Part product by using a p control chart, and a causal diagram.The results of the study conclude that the calculation of the percentage level of defect in the Base Single Part component using the p control chart, there are 3 percentage points that are outside the control limit, namely in August with a disability percentage of 15.11%, in September 10.32%, and October 15.60%. Factors causing defects in Base Single Part components consist of 3 factors, human factors, method factors and machine factors. The human factor is workers who are less productive. Furthermore, the method factor is regarding the fluctuating production schedule and the last cause is the machine factor that lacks maintenance.


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
A. Mohan Kumar ◽  
R. Rajasekar ◽  
P. Manoj Kumar ◽  
R. Parameshwaran ◽  
Alagar Karthick ◽  
...  

The drilling process is the most essential in the final assembly of the mechanical parts. Natural fiber based composites replace traditional materials due to their advantages, such as strength to weight ratio, availability, and environmental hazards. In this work, an attempt has been made to find the minimum thrust force produced by the drill tool at specified drilling process parameters. The drilling process parameters used for the investigation are rotational speed, tool feed, and resins. The spindle speed and feed rate are selected with three levels. Three resin materials were used, namely, epoxy, polyester, and vinyl ester. Taguchi’s L27 orthogonal array was implemented. The result shows that the candlestick drill bit generated lesser thrust force at the specified drilling process parameters, followed by the twist and step cone drill bits.


Machines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 295
Author(s):  
José Ignacio Rojas-Sola ◽  
Gloria del Río-Cidoncha ◽  
Rafael Ortíz-Marín ◽  
Justino Trenas-Arbizu

This article shows an approach to the three-dimensional modelling of a sugarcane mill and its associated steam engine, designed by the Robey and Co. engineering company in 1869. In order to obtain the 3D CAD model of said invention, CATIA V5 R20 software has been employed. Various sources of starting material, from the basis of this research and found in the process of searching for relevant information, provide information on the main elements, operating conditions, and mechanism of the machine. Thanks to the three-dimensional geometric modelling carried out, it has been possible to explain, in detail, both its operation and the final assembly of the invention through the assemblies of its different subsets, thereby obtaining a virtual recreation that shows its operation. Likewise, a study has been carried out, from a mechanical engineering viewpoint, of the gear train that transmitted the movement, in order to ascertain the compression force exerted on the sugar cane. This research, related to industrial archaeology, therefore, enables the reader to understand a machine that constituted a milestone in the sugarcane industry, while paying tribute to the English engineer, Robert Robey.


2021 ◽  
Author(s):  
Michelangelo Martini ◽  
Jonathan A. Anaya Guarneros ◽  
Luigi Solari ◽  
Alejandra Bedoya ◽  
Mildred Zepeda‐Martínez ◽  
...  

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