scholarly journals Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark

2021 ◽  
Vol 332 ◽  
pp. 08005
Author(s):  
Aminah Dalimunthe ◽  
Dewi Pertiwi ◽  
Mahatir Muhammad ◽  
Denny Satria
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Litsea Cubeba ◽  
Ferric Reducing Antioxidant Power ◽  
Phenolic And Flavonoid

Free radicals also play a role in the pathology of various degenerative diseases such as cancer, rheumatism, coronary heart disease, cataracts, and others. Free radicals can come from within the body (endogenous) and outside the body (exogenous). Litsea cubeba (Lour,) is a Lauraceae family plant which have contents volatile oils which used as antimicrobial, anticancer on breast cancer, pesticide, antideppressants, antiinflammation, antioxidant, and neuro pharmacology. The extract was prepared using water with the soxhletation method. The antioxidant activity was determined with the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-Azinobis [3-ethylbenzothiazoline-6)-sulfonic acid] -diammonium salt (ABTS) and Ferric Reducing Antioxidant Power (FRAP) methods. Total flavonoid and total phenolic content were determined with colorimetric methods. Antioxidant activity measured as IC50 was 23.37 ± 0.42 µg/mL; 111.21 ± 0.42 and 109.01 ± 0.28 respectively. The extract was found to contain high levels of total phenolic (282.93 ± 0.33 mg GAE/g) and total flavonoid 7.49 ± 0.51 mg QE/g). The results reveal that ethanol extract of Litsea cubeba Lour. Bark has antioxidant potential. The further analysis is to isolation antioxidant compound.

scholarly journals PENENTUAN KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN DADAP MERAH (Erythrina fusca Lour) SECARA SPEKTROFOTOMETRI UV-Vis

2019 ◽  
Vol 11 (1) ◽  
pp. 09-16
Author(s):  
Epi Supri Wardi ◽  
Zulkarni R Zulkarni R ◽  
Desy Nurdianti
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Determination of total phenolate and antioxidant activity of red leaf extract (Erythrina fusca Lour) was done by UV-Vis spectrophotometry. This study aims to determine the total phenolic content and antioxidant activity of hexane, ethyl acetate and ethanol extract. The extracts were prepared using a non-polar-maseration method with hexane, ethyl acetate and ethanol solvents. The results showed total phenolic concentration using the Folin-Ciocalteu method were 0.412 g/100 g in the hexane extract, 1.782 g/100 g in the ethyl acetate extract and 5.455 g/100 g in the ethanol extract. Antioxidant activity conducted by using FRAP method (Ferric Reducing Antioxidant Power) were obtained 0,682 mmol Fe (II)/100 g at hexane extract, 5,186 mmol Fe (II)/100 g at ethyl acetate extract and 10,591 mmol Fe (II)/100 g on the ethanol extract. The antioxidant activity of gallic acid as standard was 44.356 g mmol Fe (II)/100g.

scholarly journals Antibacterial and antioxidant activities of ethanol extract of Artocarpus lacucha Buch-Ham. leaves against Propionibacterium acne

2021 ◽  
Vol 332 ◽  
pp. 08007
Author(s):  
Panal Sitorus ◽  
Mahatir Muhammad ◽  
Henni Cintya ◽  
and Denni Satria
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Degenerative Diseases ◽  
Inhibitor Concentration ◽  
Propionibacterium Acne

Antioxidants can neutralize free radicals that can affect biological systems in the body that can cause degenerative diseases. Antibacterial active molecules have been identified from plants, and these chemicals are being utilized to develop new and promising treatments. The Mobe plant (Artocarpus lacucha Buch-Ham.) is a species of Artocarpus member of the Moraceae family. Includes a high proportion of phenolic compounds (flavonoids and phenolic acid). This research obtained to know about the biological activity of Artocarpus lacucha Buch-Ham and their total flavonoid and total phenolic content. The extract was prepared using the soxhletation technique using ethanol as the solvent. Antibacterial activity was determined using minimum inhibitor concentration. Propionibacterium acne can be inhibited at concentrations ranging from 300 ppm to 25 ppm. The DPPH technique was used to assess antioxidant activity. The samples’ total flavonoid and phenolic content were measured colorimetrically. The DPPH test revealed an antioxidant activity of 23.33 g/mL, which was used to compute the IC50. The extract’s total phenolic (117.44 mg GAE/g) and flavonoid (3.54 mg QE/g) content were determined to be high.

scholarly journals Effect of loloh sembung (Blumea balsamifera) maturity stage on antioxidant activity

2019 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
I Gusti Ayu Wita Kusumawati ◽  
I Nengah Reyunika ◽  
Ida Bagus Agung Yogeswara ◽  
I Gede Mustika ◽  
I Made Wisnu Adi Putra ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Maturity Stage ◽  
Antioxidant Power ◽  
Positive Correlation ◽  
Leaf Maturity ◽  
Maturity Stages ◽  
Young Leaves ◽  
Ferric Reducing Antioxidant Power

<p><strong>ABSTRAK</strong></p><p><strong></strong> <strong>Latar </strong>belakang<strong>:</strong> Loloh sembung (Blumea balsamifera) adalah minuman tradisional masyarakat Bali yang digunakan untuk mengobati penyakit. Perbedaan kematangan daun sembung akan mempengaruhi aktivitas antioksidan loloh sembung.</p><p><strong>Tujuan:</strong> Penelitian ini bertujuan untuk menentukan aktivitas antioksidan loloh sembung yang dibuat dari berbagai jenis kematangan daun sembung yang diekstrak dengan menggunakan pelarut air.</p><p><strong>Metode:</strong> Serbuk daun sembung pada tingkat kematangan yang berbeda (muda, dewasa dan tua) direbus untuk menghasilkan loloh sembung. Analisis kandungan antioksidan meliputi analisis total fenolik, total kandungan flavonoid dan aktivitas antioksidan ferric reducing antioxidant power (FRAP).</p><p><strong>Hasil:</strong> Hasil analisis menunjukkan bahwa daun sembung dengan tingkat kematangan tua menunjukkan kandungan total fenolik dan aktivitas antioksidan yang tinggi dibandingkan dengan daun dewasa dan muda, yaitu masing-masing sebesar 0,85±0,005 GAE/g dan 0,66 ±0,003 mmol Fe2+/g sampel. Sedangkan, daun dengan tingkat kematangan dewasa menunjukkan total flavonoid yang tinggi, yaitu sebesar 0,39±0,006 QE/g. Berdasarkan korelasi Pearson, perbedaan tingkat kematangan daun menunjukkan korelasi positif dengan kandungan total fenolik, total flavonoid dan aktivitas antioksidan (FRAP).</p><p><strong>Kesimpulan:</strong> Perbedaan tingkat kematangan daun sembung menunjukkan korelasi positif dengan kandungan total fenolik, total flavonoid dan aktivitas antioksidan (FRAP). Penelitian ini menunjukkan bahwa loloh sembung berpotensi sebagai minuman fungsional yang memanfaatkan kearifan lokal.</p><p><strong> KATA KUNCI</strong>: tingkat kematangan daun, total fenolik, total flavonoid, FRAP</p><p> </p><p><strong>ABSTRACT</strong></p><p><strong> Background:</strong> A Loloh sembung (Blumea balsamifera) is a traditional herbal drink from Bali and widely used to treat several diseases by Balinese people. Sembung leaf at different maturity stages would affect antioxidant activity of loloh sembung.</p><p><strong>Objectives:</strong> The objective of the research was determined antioxidant activity of loloh sembung prepare from different maturity stages of sembung leaf and extracted using water.</p><p><strong>Methods:</strong> Sembung leaves powder at different maturity stages (young, mature and old) was boiled to produce loloh sembung. The analyses of antioxidant activity of loloh sembung included total phenolic content (TPC), total flavonoid content (TFC) and ferric reducing antioxidant power (FRAP).</p><p><strong>Results:</strong> The results showed that old leaves were significantly higher in TPC and FRAP values compare to mature and young leaves, i.e 0.8575±0.005 GAE/g and 0.6625±0.003 mmol Fe2+/g sample respectively. However, the mature leaves revealed significantly high TFC, i.e 0.3972±0.006 QE/g. Based on Pearson’s correlation coefficient, the difference of maturity stage exhibited positive correlation with TPC, TFC and FRAP.</p><p><strong>Conclusion:</strong> The different of maturity stage exhibite showed positive correlation with TPC, TFC and FRAP. This study suggested that loloh sembung had a promising prospect as functional drink based on local wisdom.</p><p><strong>KEYWORDS:</strong> leaf maturity stage, loloh sembung, total flavonoids, total phenolic (italic)</p>

scholarly journals PENENTUAN KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN DADAP MERAH (Erythrina fusca Lour) SECARA SPEKTROFOTOMETRI UV-Vis

2019 ◽  
Vol 11 (1) ◽  
pp. 09-16
Author(s):  
Epi Supri Wardi ◽  
Zulkarni R Zulkarni R ◽  
Desy Nurdianti
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Determination of total phenolate and antioxidant activity of red leaf extract (Erythrina fusca Lour) was done by UV-Vis spectrophotometry. This study aims to determine the total phenolic content and antioxidant activity of hexane, ethyl acetate and ethanol extract. The extracts were prepared using a non-polar-maseration method with hexane, ethyl acetate and ethanol solvents. The results showed total phenolic concentration using the Folin-Ciocalteu method were 0.412 g/100 g in the hexane extract, 1.782 g/100 g in the ethyl acetate extract and 5.455 g/100 g in the ethanol extract. Antioxidant activity conducted by using FRAP method (Ferric Reducing Antioxidant Power) were obtained 0,682 mmol Fe (II)/100 g at hexane extract, 5,186 mmol Fe (II)/100 g at ethyl acetate extract and 10,591 mmol Fe (II)/100 g on the ethanol extract. The antioxidant activity of gallic acid as standard was 44.356 g mmol Fe (II)/100g.

scholarly journals Phytochemicals and Antioxidant Properties of Solvent Extracts from Purslane (Portulaca oleracea L.): A Preliminary Study

2020 ◽  
pp. 1-12 ◽  
Author(s):  
Mahmood Habibian ◽  
Ghorbanali Sadeghi ◽  
Ahmad Karimi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin E ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Scavenging Efficiency

A systematic study was conducted about the solvent effect on the purslane total phenolic, flavonoid, alkaloid, carotenoid, saponin, vitamin E, and vitamin C contents as well as to evaluate their contribution to overall antioxidant activity. Among three solvents investigated, water could extract the highest levels of solid and saponin contents, whereas methanol was more effective for obtaining phenolic, flavonoid, and alkaloid compounds than other solvents. Meanwhile, ethanol was the best choice for extracting carotenoids and vitamin E. In various experimental models including DPPH radical scavenging activity, ABTS+ radical scavenging activity, and ferric reducing antioxidant power, methanol extract consistently showed significantly greater antioxidant activity than other extracts, while ethanol extract provided the most efficient inhibiting activity against ferrous chloride-induced oxidation of linoleic acid. In addition, the scavenging efficiency of ethanol extract toward DPPH and ABTS+ radicals was significantly higher than the water extract, and vice versa was the case for ferric reducing antioxidant power. Overall, methanol was found to be the most favorable solvent for the extraction of phenolic, flavonoid, and alkaloid compounds that are promising as natural antioxidants. However, different solvents may be used to extract specific antioxidant compounds with distinctive antioxidant activity.

scholarly journals A Promising Nutraceutical Eriodictyon californicum, a “Holy Herb,” with its Healing Abilities against Oxidative Stress

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 64
Author(s):  
Allie Richards ◽  
Savita Chaurasia
Keyword(s):  
Hydroxyl Radicals ◽  
Ethanol Extract ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Daily Diet ◽  
Ferric Reducing Antioxidant Power ◽  
Eriodictyon Californicum ◽  
Phenolic And Flavonoid

Antioxidant-rich natural products can be included in the daily diet and represent a growing range of nutraceuticals. In search of effective nutraceutical agents, we studied the antioxidant potential of an herb, E. californicum, also known as yerba santa or “holy herb.” Ethanol extract of E. californicum leaves were screened for important classes of bioactive molecules. Total phenolic and flavonoid contents were quantified. Antioxidant capacity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and ferric reducing/antioxidant power (FRAP) assay. To explore the mechanism of action for antioxidant activity, the effect of the extract was studied on superoxide and hydroxyl radicals. Qualitative studies determined the presence of saponins, phlobatannins, phenols, tannins, terpenoids, cardiac glycosides, and steroids, and flavonoids in yerba santa. The leaves were found to be rich in phenol content (78.58 ± 0.016 μg GAE/mg) and flavonoid content (6.76 ± 0.003 μg QE/mg). At a concentration of 1.0 mg/mL, the extract showed 93.39% inhibition of DPPH radicals, 57.36% inhibition of superoxide radicals, and 80.89% inhibition of hydroxyl radicals. This study reveals that E. californicum leaves are a rich source of antioxidants and can be used as a nutraceutical.

scholarly journals Effect of Parinari curatellifolia Peel Flour on the Nutritional, Physical and Antioxidant Properties of Biscuits

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1262
Author(s):  
Shonisani Eugenia Ramashia ◽  
Felicia Matshepho Mamadisa ◽  
Mpho Edward Mashau
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Polyphenolic Compounds ◽  
Total Phenolic ◽  
Crude Fibre ◽  
Yellowness Index ◽  
Antioxidant Power ◽  
Parinari Curatellifolia ◽  
Ferric Reducing Antioxidant Power ◽  
The Impact

This study investigated the impact of Parinari curatellifolia peel flour on the nutritional, physical and antioxidant properties of formulated biscuits. Biscuits enriched with 5%, 10%, 15% and 20% of Parinari (P). curatellifolia peel flour were formulated and characterised. Thermal, physicochemical, polyphenolic compounds and antioxidant properties of flour and biscuits were determined. The incorporation of P. curatellifolia peel flour significantly increased (p < 0.05) thermal properties (onset, peak and conclusion temperatures) of flour. However, enthalpy of gelatinisation, viscosity and pH of flour samples decreased. Nutritional analysis revealed an increase in ash (0.74% to 2.23%) and crude fibre contents (0.39% to 2.95%) along with an increase of P. curatellifolia peel flour levels. Protein content and carbohydrates decreased while moisture content was insignificantly affected by the addition of P. curatellifolia peel flour. The L*, b* values and whiteness index of formulated biscuits decreased while parameter a* value (10.76 to 21.89) and yellowness index (69.84 to 102.71) decreased. Physical properties such as diameter (3.57 mm to 3.97 mm), spread ratio (2.67 to 3.45) and hardness (1188.13 g to 2432.60 g) increased with the inclusion levels of peel flour while weight and thickness decreased. The inclusion of P. curatellifolia improved the polyphenolic compounds and antioxidant activity of biscuits with values of total flavonoids content ranging from 0.028 to 0.104 mg CE/g, total phenolic content increasing from 20.01 mg to 48.51 mg GAE/g, ferric reducing antioxidant power (FRAP) increasing from 108.33 mg to 162.67 mg GAE/g and DPPH (2,2-diphenyl-1-picrylhydrazyl) from 48.70% to 94.72%. These results lead to the recommendation of the utilisation of P. curatellifolia peel flour to enhance the nutritional value, polyphenolic compounds and antioxidant activity of bakery products such as biscuits.

scholarly journals Phytochemicals and Antioxidant Activity in Oat-Buckwheat Dough and Cookies with Added Spices or Herbs

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2267
Author(s):  
Małgorzata Starowicz ◽  
Saruhan Arpaci ◽  
Joanna Topolska ◽  
Małgorzata Wronkowska
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Weight Basis ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Wide Range ◽  
Confectionery Products ◽  
Ferric Reducing Antioxidant Power ◽  
Phytochemical Contents

The aim of this study was to determine the phytochemicals and antioxidant activity in oat-buckwheat doughs and cookies with the addition of ten selected spices or herbs (2 g/100 g flours weight basis). The used spices and herbs, as was expected, showed a wide range of bioactive molecules, namely phenolic acids and flavonoids, and they are a rich source of components with antioxidant potential. All analysed oat-buckwheat dough showed higher antioxidant activity potential and higher total phenolic content (TPC) compared to cookies. The highest TPC was found in clove, both dough and cookies, with its addition showing the highest ferric reducing antioxidant power. Generally, cookies with the addition of spice/herbs showed higher phytochemical contents and antioxidant activity compared to oat-buckwheat cookies without the condiment. The technology of obtaining confectionery products, like oat-buckwheat cookies, that will favor the protection of bioactive compounds should still be improved.

scholarly journals Comparative Evaluation of Argania spinosa and Olea europaea Leaf Phenolic Compounds and their Antioxidant Activity

Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Total Flavonoids Content

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.

scholarly journals Changes of antioxidant activity in honey after heat treatment

2013 ◽  
Vol 31 (No. 6) ◽  
pp. 601-606 ◽  
Author(s):  
G. Šarić ◽  
K. Marković ◽  
D. Vukičević ◽  
E. Lež ◽  
M. Hruškar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Significant Difference ◽  
Acacia Honey ◽  
Different Temperatures ◽  
Ferric Reducing Antioxidant Power

We determined how the antioxidant activity and total phenolic content of honey changed after being subjected to a high temperature. Antioxidant activity was determined using two methods &ndash; FRAP (ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. Total phenolic content was determined by modified Folin-Ciocalteu method. The research was conducted on 31 samples of acacia honey and 8 samples of chestnut honey. All measurements were done at two temperatures &ndash; at 23&deg;C (room temperature) and after 5 min of heating at 95&deg;C. The obtained results show uneven changes of antioxidant activity and total phenolic content among individual samples, i.e. in some samples antioxidant activity decreased after heating, while in others it increased. The same applies to the total phenolic content. Statistical analysis of the results (t-test) showed no statistically significant differences between the results measured at two different temperatures (P &gt; 0.05) in all three methods used, and in both types of honey. The only statistically significant difference (P &lt; 0.05) was observed when using DPPH method in acacia honey.

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