scholarly journals Kinetic of Biomass Growth and Protein Formation on Rice Bran Fermentation Using Rhizopus oryzae

2018 ◽  
Vol 156 ◽  
pp. 01023 ◽  
Author(s):  
Andhika Sukma ◽  
Bakti Jos ◽  
Siswo Sumardiono
Keyword(s):  
Solid State ◽  
Rice Bran ◽  
Solid State Fermentation ◽  
Nutritional Value ◽  
Rhizopus Oryzae ◽  
Ash Content ◽  
Rice Grain ◽  
Biomass Growth ◽  
Rice Husks ◽  
Tray Bioreactor

Rice bran is the outermost part of the rice grain wrapped in rice husks. Rice bran contains macro and micro nutrients that are bound to fibers that affect psychochemical properties when used in food products. The purpose of this study was to increase the nutritional value of rice bran with solid state fermentation using Rhizopus oryzae FNCC 6011 in tray bioreactor at 30°C for 120 h. The results showed an increase in protein, fat and ash content by 58.5%, 124.5%, and 18.6%, respectively, while carbohydrate and fiber content decreased respectively by 25.6% and 51.2%. The deceleration model successfully depicts the profile of biomass growth and improved protein. The kinetic parameters obtained k, A, and YPX were 0.0536/h, 5.2537, and 0.1821 g/g, respectively.

scholarly journals Optimization of solid‐state fermentation condition for crude protein enrichment of rice bran using Rhizopus oryzae in tray bioreactor

2021 ◽  
Vol 26 (1) ◽  
pp. 33
Author(s):  
Andhika Cahaya Titisan Sukma ◽  
Herawati Oktavianty ◽  
Siswo Sumardiono
Keyword(s):  
Solid State ◽  
Rice Bran ◽  
Crude Protein ◽  
Rhizopus Oryzae ◽  
P Value ◽  
Substrate Thickness ◽  
Fermentation Condition ◽  
Protein Enrichment ◽  
Fermentation Temperature ◽  
Tray Bioreactor

Enhancement of crude protein content in rice bran with the solid‐state fermentation method in tray bioreactor using Rhizopus oryzae FNCC 6011 has been investigated. This research aimed to optimize the fermentation condition using the response surface methodology (RSM). The central composite design (CCD) with three independent variables, including substrate thickness (1 to 3 cm), fermentation temperature (28 to 32 °C), and nutrient concentration of KH2PO4 (2 to 6 g/L) used to determine the crude protein enrichment. The quadratic model has successfully described the effect of variable interactions on responses very well as indicated by the F value and p‐value are 11.20 and 0.0041, respectively. The multiple correlation coefficients (R2) of 0.9438 indicated that 94.38% of the model data has approached the actual data with a deviation of 5.62%. The interaction between the variable substrate thickness and the fermentation temperature is the most influential variable on the crude protein enrichment of rice bran, indicated by the highest F value of 24.08 and the lowest p‐value of 0.0027. The highest protein increase of 62.51% was obtained at 2 cm substrate thickness, fermentation temperature of 30 °C, and KH2PO4 concentration of 4 g/L.

scholarly journals Solid-state fermentation for the enrichment and extraction of proteins and antioxidant compounds in rice bran by Rhizopus oryzae

2012 ◽  
Vol 55 (6) ◽  
pp. 937-942 ◽  
Author(s):  
Larine Kupski ◽  
Eliane Cipolatti ◽  
Meritaine da Rocha ◽  
Melissa dos Santos Oliveira ◽  
Leonor de Almeida Souza-Soares ◽  
...  
Keyword(s):  
Solid State ◽  
Rice Bran ◽  
Solid State Fermentation ◽  
Rhizopus Oryzae ◽  
Antioxidant Compounds

scholarly journals The effect of substrate composition on the activity of amylase and cellulase by Trichoderma harzianum strains under solid state fermentation

2019 ◽  
Vol 1 (2) ◽  
pp. 41-48
Author(s):  
Toga Pangihotan Napitupulu ◽  
Nathaniel Reinhart Silaban ◽  
Atit Kanti ◽  
I Made Sudiana
Keyword(s):  
Solid State ◽  
Rice Bran ◽  
Solid State Fermentation ◽  
Trichoderma Harzianum ◽  
Cellulase Activity ◽  
Cellulolytic Enzyme ◽  
Rice Grain ◽  
White Rice ◽  
Plant Growth Promoting ◽  
Fungal Consortium

Trichoderma harzianum is a filamentous fungus that has been known to have biocontrol and plant growth-promoting ability. However, the propagation of this fungus particularly through solid state fermentation (SSF) and characterization of its enzyme activity as one the indicator of quality of fermentation process are still needed to be explored further. Rice grain and its derivative products have economically as well as nutrient composition features beneficial as substrates of fungal propagation through SSF. Therefore, the aim of this study was to investigate the effect of white rice, rice bran, and combination of white rice and rice bran on the activity of amylase and cellulase by Trichoderma harzianum strains under SSF. Two strains of the fungus, InaCC F116 and InaCC F89, as well as their consortium were employed as fungal inoculants. After closed fermentation in dark chamber at 30 ± 1°C for 7 days, the activity of amylolytic as well as cellulolytic enzyme was assayed. The result showed that the presence of rice bran as a substrate increased the activity of crude amylase and crude cellulase. In all substrates, the strain F116 has low activity of both enzymes. The fungal consortium improves the activity of crude enzymes in all substrates. Therefore, the amylase and cellulase activity by T. harzianum in SSF condition were strain- as well as substrate-dependent.

scholarly journals Optimization of Cultural Conditions for Solid State Fermentation of Amylase Production by Aspergillus species

2013 ◽  
Vol 14 (1) ◽  
pp. 67-74
Author(s):  
Bina Gautam ◽  
Tika B Karki ◽  
Om Prakash Panta
Keyword(s):  
Solid State ◽  
Rice Bran ◽  
Solid State Fermentation ◽  
Food Industry ◽  
Raw Materials ◽  
Morphological Characteristics ◽  
Amylolytic Enzyme ◽  
Cultural Conditions ◽  
Amylase Production ◽  
Selection Of

Amylase is an amylolytic enzyme used in food industry which is generally produced by Aspergillus spp. under solid state fermentation. The present study is concerned with the isolation, screening and selection of suitable strains of Aspergillus spp. and optimization of cultural conditions for the biosynthesis of amylase. Rice and wheat brans were used as substrates which are readily available inexpensive raw materials for amylase production. From 85 samples of rice and wheat grains, 55 colonies obtained on potato dextrose agar (PDA) were suspected to be Aspergillus oryzae and only 35 colonies possessed the morphological characteristics similar to that of A. oryzae indicating the isolates were most likely the strains of A. oryzae. Of all the fungal isolates of Aspergillus spps., Asp.31 gave maximum production of amylase (720.782 IUgds-1) in solid state fermentation media. This strain was selected as a parental strain for optimization for cultural conditions. The obtained data were analyzed using SPSS- 11.5 program. Of all the substrates (rice bran, wheat bran and their mixture), rice bran was the best for producing amylase of highest activity 611.614 IUgds-1.The highest enzyme activity of 698.749 IUgds-1 was observed at 50% initial moisture level of the substrate. The optimum temperature was 25°C for producing the crude amylase enzyme with amylase activity of 577.757 IUgds-1. Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 67-74 DOI: http://dx.doi.org/10.3126/njst.v14i1.8924

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