Larvicidal activity of Ocimum campechianum, Ocotea quixos and Piper aduncum essential oils against Aedes aegypti

Aedes aegypti, the main arbovirus vector of the Yellow fever, Dengue, Chikungunya and Zika viruses, is widely distributed in tropical and subtropical areas throughout the world. Preventive control efforts have been implemented worldwide aimed at reducing its impact on human health. The recent reduction of chemicals available for vector control due to their negative impact on the environment and human health and the increase in mosquito resistance to insecticides have driven the research community to identify and evaluate sustainable alternatives to synthetic insecticides. In this study, the potential larvicidal effect of essential oils extracted from Ocimum campechianum, Ocotea quixos and Piper aduncum were tested in vitro. GC and GC–MS analyses showed that the main compounds were eugenol (18%), 1,8-cineole (39%) and dillapiole (48%), respectively. Susceptibility to essential oils was measured according to the WHO protocol. After 24 h, the mean percentage mortality ranged from 2.7 to 100% for P. aduncum, from 2.2 to 100% for O. campechianum, and from 2.9 to 100% for O. quixos. The highest potential was displayed by P. aduncum, followed by O. campechianum and O. quixos, with LC50 values of 25.7, 69.3 and 75.5 ppm, respectively. The rapid and effective larvicidal activity of these three oils led us to consider these results to be promising, also considering the possibility of local cultivation of these plants in tropical and subtropical areas and the simple technology for their large-scale preparation and production. Further studies are needed to evaluate the individual components and their activity as larvicides.

Download Full-text

Larvicidal Activity of Essential Oils From Piper Species Against Strains of Aedes aegypti (Diptera: Culicidae) Resistant to Pyrethroids

Frontiers in Plant Science ◽

10.3389/fpls.2021.685864 ◽

2021 ◽

Vol 12 ◽

Author(s):

Adalberto Alves Pereira Filho ◽

Grasielle C. D‘Ávila Pessoa ◽

Lydia F. Yamaguchi ◽

Mariana Alves Stanton ◽

Artur M. Serravite ◽

...

Keyword(s):

Aedes Aegypti ◽

Essential Oils ◽

Larvicidal Activity ◽

Positive Control ◽

World Health ◽

Vector Insect ◽

Insect Populations ◽

Resistant Strains ◽

Health Organization ◽

Piper Aduncum

The continuous and indiscriminate use of insecticides has been responsible for the emergence of insecticide resistant vector insect populations, especially in Aedes aegypti. Thus, it is urgent to find natural insecticide compounds with novel mode of action for vector control. The goal of this study was to investigate the larvicidal activity of essential oils (EOs) from Piper species against A. aegypti characterized as resistant and susceptible strains to pyrethroids. The EOs from leaves of 10 Piper species were submitted to the evaluation of larvicidal activity in populations of A. aegypti in agreement with the (World Health Organization, 2005) guidelines. The resistance of the strains characterized by determining the lethal concentrations (LCs) with the insecticide deltamethrin (positive control). The major compounds of the EOs from Piper species was identified by GC-MS. The EOs from Piper aduncum, P. marginatum, P. gaudichaudianum, P. crassinervium, and P. arboreum showed activity of up to 90% lethality at 100 ppm (concentration for screening). The activities of the EOs from these 6 species showed similar LCs in both susceptible strain (Rockefeller) and resistant strains (Pampulha and Venda Nova) to pyrethroids. The major compounds identified in the most active EO were available commercially and included β-Asarone, (E)-Anethole, (E)-β-Caryophyllene, γ-Terpinene, p-Cymene, Limonene, α-Pinene, and β-Pinene. Dillapiole was purified by from EO of P. aduncum. The phenylpropanoids [Dillapiole, (E)-Anethole and β-Asarone] and monoterpenes (γ-Terpinene, p-Cymene, Limonene, α-Pinene, and β-Pinene) showed larvicidal activity with mortality between 90 and 100% and could account for the toxicity of these EOs, but the sesquiterpene (E)-β-Caryophyllene, an abundant component in the EOs of P. hemmendorffii and P. crassinervium, did not show activity on the three populations of A. aegypti larvae at a concentration of 100 ppm. These results indicate that Piper's EOs should be further evaluated as a potential larvicide, against strains resistant to currently used pesticides, and the identification of phenylpropanoids and monoterpenes as the active compounds open the possibility to study their mechanism of action.

Download Full-text

Chemical Composition of Heracleum pastinacifolium subsp. transcaucasicum and subsp. incanum Essential Oils, and Their Biting Deterrent and Larvicidal Activity Against Aedes aegypti

Planta Medica ◽

10.1055/s-0032-1307597 ◽

2012 ◽

Vol 78 (05) ◽

Cited By ~ 2

Author(s):

N Tabanca ◽

G Özek ◽

A Ali ◽

A Duran ◽

E Hamzaoğlu ◽

...

Keyword(s):

Chemical Composition ◽

Aedes Aegypti ◽

Essential Oils ◽

Larvicidal Activity ◽

Heracleum Pastinacifolium

Download Full-text

Larvicidal Activity of essential oils from Brazilian plants against Aedes aegypti L.

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762004000500015 ◽

2004 ◽

Vol 99 (5) ◽

pp. 541-544 ◽

Cited By ~ 178

Author(s):

Eveline Solon Barreira Cavalcanti ◽

Selene Maia de Morais ◽

Michele Ashley A Lima ◽

Eddie William Pinho Santana

Keyword(s):

Aedes Aegypti ◽

Essential Oils ◽

Larvicidal Activity

Download Full-text

In Vitro Evaluation of Acute Toxicity of Five Citrus spp. Essential Oils towards the Parasitic Mite Varroa destructor

Pathogens ◽

10.3390/pathogens10091182 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1182

Author(s):

Roberto Bava ◽

Fabio Castagna ◽

Cristian Piras ◽

Ernesto Palma ◽

Giuseppe Cringoli ◽

...

Keyword(s):

Essential Oils ◽

Honey Bee ◽

Varroa Destructor ◽

Negative Impact ◽

Positive Control ◽

Citrus Limon ◽

Citrus Paradisi ◽

Bee Health ◽

Citrus Spp

Varroa destructor is the most important ectoparasitic mite of honey bees that has a negative impact on bee health and honey production. The control programs are mainly based on the use of synthetic acaricides that are often administered indiscriminately. All this has led to drug resistance that now represent a great concern for honey bee farming. The research for alternative products/methods for mites’ control is now mandatory. The aim of this study was to test whether Citrus spp. essential oils could diminish the growth of the V. destructor mite. In Calabria (southern Italy), plants of the Citrus genus are very common and grow both spontaneously and cultured. The essential oils used in this study were extracted from bergamot (Citrus bergamia), grapefruit (Citrus paradisi), lemon (Citrus limon), orange (Citrus sinensis), and mandarin (Citrus reticulata) by hydrodistillation. Every EO was in vitro tested against V. destructor. Each experimental replicate was performed using 35 viable adult female mites (5 for each EO) collected the same day from the same apiary and included negative controls (5 individuals exposed to acetone only) and positive controls (5 individuals exposed to Amitraz diluted in acetone). The essential oils (Eos) were diluted (0.5 mg/mL, 1 mg/mL, and 2 mg/mL) in HPLC grade acetone to obtain the working solution to be tested (50 µL/tube). Mite mortality was manually assessed after 1 h exposure under controlled conditions. The essential oils that showed the best effectiveness at 0.5 mg/mL were bergamot, which neutralized (dead + inactivated) 80% (p ≤ 0.001) of the parasites; grapefruit, which neutralized 70% (p ≤ 0.001); and lemon, which neutralized 69% of them. Interestingly, the positive control (Amitraz) at the same concentration neutralized 60% of the parasites. These results demonstrate that Calabrian bergamot, grapefruit, and lemon Eos consistently reduced V. destructor viability and open the possibility for their utilization to control this parasite in honey bee farming.

Download Full-text

Comparative Larvicidal Activity of Essential Oils from Three Medicinal Plants against Aedes aegypti L.

Chemistry & Biodiversity ◽

10.1002/cbdv.200900260 ◽

2010 ◽

Vol 7 (11) ◽

pp. 2801-2807 ◽

Cited By ~ 19

Author(s):

Flávia P. Freitas ◽

Silvério P. Freitas ◽

Gloria C. S. Lemos ◽

Ivo J. C. Vieira ◽

Geraldo A. Gravina ◽

...

Keyword(s):

Medicinal Plants ◽

Aedes Aegypti ◽

Essential Oils ◽

Larvicidal Activity

Download Full-text

Exposure to airborne gold nanoparticles: a review of current toxicological data on the respiratory tract

Journal of Nanoparticle Research ◽

10.1007/s11051-020-04966-9 ◽

2020 ◽

Vol 22 (8) ◽

Author(s):

Barbara De Berardis ◽

Magda Marchetti ◽

Anna Risuglia ◽

Federica Ietto ◽

Carla Fanizza ◽

...

Keyword(s):

Gold Nanoparticles ◽

Human Health ◽

Large Scale ◽

Current Knowledge ◽

Engineered Nanoparticles ◽

In Vivo Studies ◽

Field Exposure ◽

The Impact

AbstractIn recent years, the introduction of innovative low-cost and large-scale processes for the synthesis of engineered nanoparticles with at least one dimension less than 100 nm has led to countless useful and extensive applications. In this context, gold nanoparticles stimulated a growing interest, due to their peculiar characteristics such as ease of synthesis, chemical stability and optical properties. This stirred the development of numerous applications especially in the biomedical field. Exposure of manufacturers and consumers to industrial products containing nanoparticles poses a potential risk to human health and the environment. Despite this, the precise mechanisms of nanomaterial toxicity have not yet been fully elucidated. It is well known that the three main routes of exposure to nanomaterials are by inhalation, ingestion and through the skin, with inhalation being the most common route of exposure to NPs in the workplace. To provide a complete picture of the impact of inhaled gold nanoparticles on human health, in this article, we review the current knowledge about the physico-chemical characteristics of this nanomaterial, in the size range of 1–100 nm, and its toxicity for pulmonary structures both in vitro and in vivo. Studies comparing the toxic effect of NPs larger than 100 nm (up to 250 nm) are also discussed.

Download Full-text

Chemical Composition and Larvicidal Activity against Aedes Aegypti of Essential Oils from Croton Zehntneri

Natural Product Communications ◽

10.1177/1934578x0700201208 ◽

2007 ◽

Vol 2 (12) ◽

pp. 1934578X0700201 ◽

Cited By ~ 9

Author(s):

Hélcio S. Santos ◽

Gilvandete M. P. Santiago ◽

João P. P. de Oliveira ◽

Angela M. C. Arriaga ◽

Délcio D. Marques ◽

...

Keyword(s):

Chemical Composition ◽

Aedes Aegypti ◽

Essential Oils ◽

Larvicidal Activity ◽

Plant Parts ◽

Main Component

The chemical composition of the essential oils from leaves, stalks and inflorescences of Croton zehntneri obtained by hydrodistillation were analyzed by GC-MS and CG-FID. E-Anethole was the main component of the essential oils of all plant parts. Essential oils of leaves, stalks, inflorescences and E-anethole were tested at different concentrations against instar III larvae of Aedes aegypti and showed LC50 values of 56.2 ± 0.3, 51.3 ± 0.3, 57.5 ± 0.1 and 69.2 ± 0.5 μg/mL, respectively.

Download Full-text

Larvicidal Activity of Myrtaceae Essential Oils and Their Components Against Aedes aegypti, Acute Toxicity on Daphnia magna, and Aqueous Residue

Journal of Medical Entomology ◽

10.1603/me10108 ◽

2011 ◽

Vol 48 (2) ◽

pp. 405-410 ◽

Cited By ~ 49

Author(s):

Hye-Mi Park ◽

Junheon Kim ◽

Kyu-Sik Chang ◽

Byung-Seok Kim ◽

Yu-Jung Yang ◽

...

Keyword(s):

Acute Toxicity ◽

Aedes Aegypti ◽

Essential Oils ◽

Daphnia Magna ◽

Larvicidal Activity ◽

Aqueous Residue

Download Full-text

Insecticidal and Biting Deterrent Activities of Magnolia grandiflora Essential Oils and Selected Pure Compounds against Aedes aegypti

Molecules ◽

10.3390/molecules25061359 ◽

2020 ◽

Vol 25 (6) ◽

pp. 1359

Author(s):

Abbas Ali ◽

Nurhayat Tabanca ◽

Betul Demirci ◽

Vijayasankar Raman ◽

Jane M. Budel ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Screening Program ◽

Negative Control ◽

Magnolia Grandiflora ◽

Plant Parts ◽

Pure Compounds ◽

Index Value

In our natural products screening program for mosquitoes, we tested essential oils extracted from different plant parts of Magnolia grandiflora L. for their insecticidal and biting deterrent activities against Aedes aegypti. Biting deterrence of seeds essential oil with biting deterrence index value of 0.89 was similar to N,N-diethyl-3-methylbenzamide (DEET). All the other oils were active above the solvent control but the activity was significantly lower than DEET. Based on GC-MS analysis, three pure compounds that were only present in the essential oil of seed were further investigated to identify the compounds responsible for biting deterrent activity. 1-Decanol with PNB value of 0.8 was similar to DEET (PNB = 0.8), whereas 1-octanol with PNB value of 0.64 showed biting deterrence lower than 1-decanol and DEET. The activity of 1-heptanol with PNB value of 0.36 was similar to the negative control. Since 1-decanol, which was 3.3% of the seed essential oil, showed biting deterrence similar to DEET as a pure compound, this compound might be responsible for the activity of this oil. In in vitro A & K bioassay, 1-decanol with MED value of 6.25 showed higher repellency than DEET (MED = 12.5). Essential oils of immature and mature fruit showed high toxicity whereas leaf, flower, and seeds essential oils gave only 20%, 0%, and 50% mortality, respectively, at the highest dose of 125 ppm. 1-Decanol with LC50 of 4.8 ppm was the most toxic compound.

Download Full-text