Flexor tendon wound healing in vitro: The effect of lactate on tendon cell proliferation and collagen production

Abstract Background The skin wound healing process is regulated by various cytokines, chemokines, and growth factors. Recent reports have demonstrated that spermine/spermidine (SPD) promote wound healing through urokinase-type plasminogen activator (uPA)/uPA receptor (uPAR) signaling in vitro. Here, we investigated whether the systemic and topical administration of SPD would accelerate the skin wound-repair process in vivo.Methods A skin wound repair model was established using C57BL/6 J mice. SPD was mixed with white petrolatum for topical administration. For systemic administration, SPD mixed with drinking water was orally administered. Changes in wound size over time were calculated using digital photography.Results Systemic and topical SPD treatment significantly accelerated skin wound healing. The administration of SPD promoted the uPA/uPAR pathway in wound sites. Moreover, topical treatment with SPD enhanced the expression of IL-6 and TNF-α in wound sites. Scratch and cell proliferation assays revealed that SPD administration accelerated scratch wound closure and cell proliferation in vitro.Conclusion These results indicate that treatment with SPD promotes skin wound healing through activation of the uPA/uPAR pathway and induction of the inflammatory response in wound sites. The administration of SPD might contribute to new effective treatments to accelerate skin wound healing.

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The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods

Asian Journal of Pharmaceutical Research and Development ◽

10.22270/ajprd.v7i3.534 ◽

1970 ◽

Vol 7 (3) ◽

pp. 14-19 ◽

Cited By ~ 1

Author(s):

Hekdin Marsius Sipayung ◽

Jansen Silalahi ◽

Yuandani Y

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Protein Expression ◽

Scratch Wound ◽

Cell Proliferation Activity ◽

Proliferation Activity ◽

Cox 2 ◽

Nih 3T3 ◽

Vegf Protein

Objectives: The objective of this study was to investigate the activity of combination of hydrolyzed VCO (HVCO) and chitosan on NIH 3T3 cell proliferation activity, NIH 3T3 cell migration, COX-2 and VEGF protein expression. Design: In vitro cytotoxic assay was determined by MTT (MicrocultureTetrazoliumTehnique) assay, cell proliferation activity was measured by calculating cell viability incubated 24 hours, 48 hours and 72 hours, wound closure percentage was tested by scratch wound healing method, expression of COX-2 protein and VEGF protein were measured by immunocytochemical method. Interventions: The variable that was intervened in this study was the concentration of HVCO and chitosan. Main Outcome Measures: The main measurements carried out in this study were the absorbance value of HVCO and chitosan which was converted into viability cell, proliferation activity, percentage of wound closure, and percentage of COX-2 and VEGF protein expression. Results: Cytotoxic activity of HVCO and chitosan resulted the best concentration at 31.25 μg/ml, scratch wound healing assay from a combination HVCO and chitosan resulted the best migration of fibroblast cells at a ratio of 1:1 with HVCO 62.5 μg/ml and chitosan 62.5 μg/ml, combination of HVCO 62.5 μg/ml and chitosan 62.5 μg/ml (1:1) increased expression of COX-2 and VEGF. Conclusion: Combination of HVCO and chitosan could increase NIH 3T3 cell migration, COX-2 and VEGF protein expression. Combination of HVCO and chitosan had better wound healing activity in vitro than single use. Keywords: Rhizomucor miehei, viability, proliferation, migration, expression

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Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies

Clinical Oral Investigations ◽

10.1007/s00784-019-03156-9 ◽

2019 ◽

Vol 24 (2) ◽

pp. 569-584 ◽

Cited By ~ 9

Author(s):

Franz-Josef Strauss ◽

Jila Nasirzade ◽

Zahra Kargarpoor ◽

Alexandra Stähli ◽

Reinhard Gruber

Keyword(s):

Systematic Review ◽

Cell Proliferation ◽

Wound Healing ◽

Bone Regeneration ◽

Therapeutic Potential ◽

In Vitro Studies ◽

Bioactive Molecules ◽

Platelet Rich Fibrin ◽

Regenerative Dentistry

Abstract Objective To systematically assess the effects of platelet-rich fibrin (PRF) on in vitro cellular behavior. Methods A systematic electronic search using MEDLINE database was performed. In vitro studies using PRF were considered and articles published up to June 31, 2018 were screened. Eligible studies were selected based on the use of human PRF. Results In total, 1746 titles were identified with the search terms, from these 37 met the inclusion criteria and were chosen for data extraction. In addition, 16 new studies, mainly published in 2019, were also included in the analysis resulting in 53 studies. No meta-analysis could be performed due to the heterogeneity of study designs. Included studies show that PRF enhances proliferation, migration, adhesion, and osteogenic differentiation on a variety of cell types along with cell signaling activation. Furthermore, PRF reduces inflammation, suppresses osteoclastogenesis, and increases the expression of various growth factors in mesenchymal cells. Summary and conclusions Despite some notable differences of the studies, the overall findings suggest a positive effect of PRF on cell proliferation, migration, adhesion, differentiation, and inflammation pointing towards a therapeutic potential in regenerative dentistry. Clinical relevance PRF serves as a reservoir of bioactive molecules to support wound healing and bone regeneration. Although the cellular mechanisms by which PRF supports the clinical outcomes remain unclear, in vitro research provides possible explanations. This systematic review aims to provide an update of the existing research on how PRF affects basic physiological processes in vitro. The overall findings suggest that PRF induces cell proliferation, migration, adhesion, and differentiation along with possessing anti-inflammatory properties further supporting its therapeutic potential in wound healing and bone regeneration.

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Equine limb and oral fibroblasts in in vitro models of cell proliferation and wound contraction

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200028581 ◽

2009 ◽

Vol 2009 ◽

pp. 19-19

Author(s):

E Watts ◽

M T Rose

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Granulation Tissue ◽

Collagen Matrix ◽

In Vitro Models ◽

Scar Formation ◽

Wound Contraction

Wound healing in horses is particularly problematic compared to other species and limb wounds often exhibit complications such as exuberant granulation tissue, poor wound contraction and unsightly scars. In comparison, oral wounds heal without scar formation. Therefore, in vitro experiments were conducted to investigate the differences between equine oral and limb fibroblasts in terms of proliferation and their ability to contract a collagen matrix, a commonly used in vitro model of wound contraction.

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Effect of human platelet concentrate, serum and PDGF on RPE cell proliferation and wound healing in vitro

Der Ophthalmologe ◽

10.1007/s003470050378 ◽

1999 ◽

Vol 96 (2) ◽

pp. 77-81 ◽

Cited By ~ 2

Author(s):

Karl-Heinrich Velhagen ◽

Alexandra Druegg ◽

Peter Rieck

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Human Platelet ◽

Platelet Concentrate

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Application ofAntrodia camphorataPromotes Rat’s Wound HealingIn Vivoand Facilitates Fibroblast Cell ProliferationIn Vitro

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/317693 ◽

2015 ◽

Vol 2015 ◽

pp. 1-14 ◽

Cited By ~ 8

Author(s):

Zahra A. Amin ◽

Hapipah M. Ali ◽

Mohammed A. Alshawsh ◽

Pouya H. Darvish ◽

Mahmood A. Abdulla

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Granulation Tissue ◽

Inflammatory Cell ◽

Biological Activities ◽

Fibroblast Cell ◽

Parasitic Fungus ◽

Antrodia Camphorata

Antrodia camphoratais a parasitic fungus from Taiwan, it has been documented to possess a variety of pharmacological and biological activities. The present study was undertaken to evaluate the potential ofAntrodia camphorataethanol extract to accelerate the rate of wound healing closure and histology of wound area in experimental rats. The safety ofAntrodia camphoratawas determinedin vivoby the acute toxicity test andin vitroby fibroblast cell proliferation assay. The scratch assay was used to evaluate thein vitrowound healing in fibroblast cells and the excision model of wound healing was testedin vivousing four groups of adultSprague Dawleyrats. Our results showed that wound treated withAntrodia camphorataextract and intrasite gel significantly accelerates the rate of wound healing closure than those treated with the vehicle. Wounds dressed withAntrodia camphorataextract showed remarkably less scar width at wound closure and granulation tissue contained less inflammatory cell and more fibroblast compared to wounds treated with the vehicle. Masson’s trichrom stain showed granulation tissue containing more collagen and less inflammatory cell inAntrodia camphoratatreated wounds. In conclusion,Antrodia camphorataextract significantly enhanced the rate of the wound enclosure in rats and promotes thein vitrohealing through fibroblast cell proliferation.

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HvRNASET2 Regulate Connective Tissue and Collagen I Remodeling During Wound Healing Process

Frontiers in Physiology ◽

10.3389/fphys.2021.632506 ◽

2021 ◽

Vol 12 ◽

Author(s):

Nicolò Baranzini ◽

Laura Pulze ◽

Gianluca Tettamanti ◽

Francesco Acquati ◽

Annalisa Grimaldi

Keyword(s):

Immune Response ◽

Wound Healing ◽

Muscle Tissue ◽

Tissue Regeneration ◽

Immune Modulation ◽

Healing Process ◽

Wound Healing Process ◽

Collagen Production

Several studies have recently demonstrated that the correct regeneration of damaged tissues and the maintaining of homeostasis after wounds or injuries are tightly connected to different biological events, involving immune response, fibroplasia, and angiogenetic processes, in both vertebrates and invertebrates. In this context, our previous data demonstrated that the Hirudo verbana recombinant protein rHvRNASET2 not only plays a pivotal role in innate immune modulation, but is also able to activate resident fibroblasts leading to new collagen production, both in vivo and in vitro. Indeed, when injected in the leech body wall, which represents a consolidated invertebrate model for studying both immune response and tissue regeneration, HvRNASET2 induces macrophages recruitment, fibroplasia, and synthesis of new collagen. Based on this evidence, we evaluate the role of HvRNASET2 on muscle tissue regeneration and extracellular matrix (ECM) remodeling in rHvRNASET2-injected wounded leeches, compared to PBS-injected wounded leeches used as control. The results presented here not only confirms our previous evidence, reporting that HvRNASET2 leads to an increased collagen production, but also shows that an overexpression of this protein might influence the correct progress of muscle tissue regeneration. Moreover, due to its inhibitory effect on vasculogenesis and angiogenesis, HvRNASET2 apparently interfere with the recruitment of the myoendothelial vessel-associated precursor cells that in turn are responsible for muscle regeneration during wound healing repair.

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A bioactive exopolysaccharide from marine bacteria Alteromonas sp. PRIM-28 and its role in cell proliferation and wound healing in vitro

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2019.03.048 ◽

2019 ◽

Vol 131 ◽

pp. 10-18 ◽

Cited By ~ 6

Author(s):

T.G. Sahana ◽

P.D. Rekha

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Marine Bacteria

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In Vitro Wound Healing Potential of Flavonoid C-Glycosides from Oil Palm (Elaeis guineensis Jacq.) Leaves on 3T3 Fibroblast Cells

Antioxidants ◽

10.3390/antiox9040326 ◽

2020 ◽

Vol 9 (4) ◽

pp. 326 ◽

Cited By ~ 1

Author(s):

Mohamad Shazeli Che Zain ◽

Soo Yee Lee ◽

Murni Nazira Sarian ◽

Sharida Fakurazi ◽

Khozirah Shaari

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Oil Palm ◽

Elaeis Guineensis ◽

Fibroblast Cells ◽

Crude Extracts ◽

Elaeis Guineensis Jacq ◽

And Migration ◽

Proliferation And Migration

Oil palm (Elaeis guineensis Jacq.) leaves (OPL) are widely available at zero cost in Southeast Asia countries, especially in Malaysia and Indonesia due to large scale oil palm plantations. OPLs contain a large amount of flavonoids in particular flavonoid C-glycosides, which are known to possess useful biological properties including antioxidant and wound healing properties. The present study aimed at evaluating the wound healing efficacy of OPL in various solvent extracts and flavonoid enriched fractions and to determine the contribution of flavonoid C-glycosides (orientin, isoorientin, vitexin and isovitexin) using in-vitro scratch assay on 3T3 fibroblast cells. Solvent crude extracts with different polarity were screened and the most active extract was subjected to acid hydrolysis. The crude and acid hydrolysed extracts were further enriched using macroporous resins, XAD7HP. UHPLC-UV/PDA and LC-MS/MS analysis were applied for identification and confirmation of flavonoid C-glycosides. The wound healing properties comprised of cell viability, cell proliferation and cell migration were studied. Allantoin was used as a positive control to compare the efficacy among the tested samples. The results revealed all OPL crude extracts, flavonoid enriched fractions and flavonoid C-glycosides were non-toxic at concentrations below 25 µg/mL and showed better cell proliferation and migration activities at low concentrations than higher concentrations.. This study also demonstrated orientin, isoorientin, vitexin and isovitexin presented in OPL extracts and flavonoid enriched fractions stimulated proliferation and migration of 3T3 fibroblast cells. Hence, these findings may pose potential therapeutic bioactive agents for wound healing by enhancing fibroblast proliferation and migration.

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