Profiles of Phenolic Acids and Triterpene Glycosides in Commercial and Cultivated Black Cohosh

Planta Medica ◽  
2019 ◽  
Vol 85 (14/15) ◽  
pp. 1160-1167
Author(s):  
Marian Bittner ◽  
Regina Schenk ◽  
Andreas Springer ◽  
Matthias F. Melzig
Keyword(s):  
Phenolic Acids ◽  
Triterpene Glycoside ◽  
Triterpene Glycosides ◽  
Black Cohosh ◽  
Herbal Products ◽  
Chromatographic Fingerprints ◽  
Actaea Racemosa ◽  
Wild Harvesting ◽  
Cultivated Plant

AbstractThe medicinal plant Actaea racemosa is a perennial, whose rhizome (black cohosh rhizome) is usually wild harvested on a multiton scale to meet market requirements. Since this North American species is increasingly endangered, cultivation is needed. Even though studies prove that cultivation is possible, it has not been widely established. This may be due to a different quality of cultivated material, which does not comply with current pharmacopoeial requirements. This study compares contents and chromatographic fingerprints of phenolic acids and triterpene glycosides in different types of black cohosh rhizomes. Commercial batches from wild harvests were compared to individual plants from the wild and from cultivation. Phenolic acidsʼ contents and profiles were generally comparable between wild harvesting and cultivation. On the contrary, the total triterpene glycoside content was significantly lower in cultivation (p ≤ 0.001). In individual plants, different profiles of triterpene glycosides occurred. Possibly, specimen or chemotype selection for cultivation would cause a shift of the triterpene glycoside profile of cultivation batches away from the common pattern found in batches from wild harvesting. Potentially, such differences have an impact on the efficacy of black cohosh herbal products, if cultivated plant material is used for manufacturing.

scholarly journals Influence of Rhizome Propagule Size on Yields and Triterpene Glycoside Concentrations of Black Cohosh [Actaea racemosa L. syn Cimicifuga racemosa (L.) Nuttal]

HortScience ◽  
2007 ◽  
Vol 42 (1) ◽  
pp. 61-64 ◽  
Author(s):  
Joe-Ann McCoy ◽  
Jeanine M. Davis ◽  
N. Dwight Camper ◽  
Ikhlas Khan ◽  
Avula Bharathi
Keyword(s):  
Triterpene Glycoside ◽  
Agricultural Research ◽  
Forest Edge ◽  
Research Field ◽  
Black Cohosh ◽  
Forest Site ◽  
Cimicifuga Racemosa ◽  
Actaea Racemosa ◽  
Shade Cloth ◽  
Disturbed Forest

Black cohosh [Actaea racemosa L. syn. Cimicifuga racemosa (L.) Nuttal] is a native North American medicinal plant traditionally harvested for its rhizomes and roots. Black cohosh products were listed in the top 10 selling herbal supplements from 2002 to 2005. As a result of increasing commercial demand, there is a need to develop propagation protocols suitable for production purposes to replace current methods of harvesting from wild populations. The objectives of this study were to 1) determine optimal rhizome propagule division size for successful regeneration, 2) analyze triterpene glycoside concentrations, 3) quantify survival rates after 3 years of production, and 4) evaluate net yield results. Experimental sites included a shade cloth structure in an agricultural research field, a shaded forest interior, and a shaded, disturbed forest edge. Plant emergence, growth, and survival were assessed at each site over a 3-year period. Optimal rhizome division size for propagation was a 10 to 30-g section originating from terminal rhizome portions. Rhizome survival averaged 97% among all treatments tested by year 3 at three sites. No differences in mean triterpene glycoside concentrations were detected between rhizome size classes or sites tested. Mean cimiracemoside concentrations ranged from 0.80 to 1.39 mg·g–1 d/w tissue, deoxyactein 0.47 to 0.92 mg·g–1, and actein 10.41 to 13.69 mg·g–1. No differences in triterpene levels were detected between flowering and nonflowering plants, nor were yields reduced. Net yields from a shade cloth production site were 9 and 17 times higher than a disturbed forest edge and forest site respectively. Black cohosh is a strong candidate for commercial propagation under adequate site selection.

Validation and optimization of qPCR method for identification of Actaea Racemosa (Black cohosh) NHPs

2020 ◽  
Author(s):  
Jeevitha Shanmughanandhan ◽  
Dhivya Shanmughanandhan ◽  
Subramanyam Ragupathy ◽  
Thomas A Henry ◽  
Steven G Newmaster
Keyword(s):  
Dietary Supplements ◽  
Raw Materials ◽  
Black Cohosh ◽  
Powder Materials ◽  
Target Species ◽  
Herbal Products ◽  
Hydrolysis Probe ◽  
Actaea Racemosa ◽  
Probe Assay ◽  
Species Specific

Abstract BACKGROUND Actaea racemosa (Black cohosh) herbal dietary supplements are commonly used to treat menopausal symptoms in women. However, there is a considerable risk of contamination of A. racemosa herbal products in the natural health product (NHP) industry, impacting potential efficacy. Authentication of A. racemosa products is challenging because of the standard, multi-part analytical chemistry methods that may be too costly and not appropriate for both raw and finished products. OBJECTIVE In this paper, we discuss about developing and validating quick alternative biotechnology methods to authenticate A. racemosa herbal dietary supplements, based on the use of a species-specific hydrolysis PCR probe assay. METHODS A qPCR based species-specific hydrolysis probe assay was designed, validated, and optimized for precisely identifying the species of interest using the following analytical validation criteria: (1) specificity (accuracy) in determining the target species ingredient, while not identifying other non-target species, (2) sensitivity in detecting the smallest amount of the target material, and (3) reliability (repeatability and reproducibility) in detecting the target species in raw materials on a real-time PCR platform. RESULTS The results show that the species-specific hydrolysis probe assay was successfully developed for the raw materials and powders of A. racemosa. The specificity of the test was 100% to the target species. The efficiency of the assay was observed to be 99%, and the reliability of the assay was 100% for the raw/starting and powder materials. CONCLUSION The method developed in this study can be used to authenticate and perform qualitative analysis of A. racemosa supplements.

Quantification of polyphenols in powdered Actaea racemosa (black cohosh) rhizomes by NIR spectroscopy

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
M Bittner ◽  
A Kraehmer ◽  
MF Melzig
Keyword(s):  
Nir Spectroscopy ◽  
Black Cohosh ◽  
Actaea Racemosa

Black Cohosh (Actaea racemosa) for the Relief of Menopausal Indications: A Review

2020 ◽  
Vol 4 (1) ◽  
pp. 62-72
Author(s):  
Anirban Adhikary ◽  
Kakoli Halder ◽  
Debmalya Ghosh ◽  
Indranil Chatterjee ◽  
Suman Kumar Nath
Keyword(s):  
Black Cohosh ◽  
Actaea Racemosa

New Monitoring Strategy for the Quality Control in the Processing Practice of Scutellariae Radix

2020 ◽  
Vol 16 (3) ◽  
pp. 303-311
Author(s):  
Qi Huang ◽  
Chunsong Cheng ◽  
Lili Li ◽  
Daiyin Peng ◽  
Cun Zhang
Keyword(s):  
Quality Control ◽  
Monitoring Strategy ◽  
Scutellariae Radix ◽  
Chromatographic Fingerprints ◽  
New Strategy ◽  
Processed Products ◽  
Main Components ◽  
Processing Techniques ◽  
Multivariate Monitoring

Background: Scutellariae Radix (Huangqin) is commonly processed into 3 products for different clinical applications. However, a simple analytical method for quality control has rarely been reported to quickly estimate the degree of processing Huangqin or distinguish differently processed products or unqualified Huangqin products. Objective: To study a new strategy for quality control in the processing practice of Huangqin. Methods: Seven kinds of flavonoids that mainly exist in Huangqin were determined by HPLC-DAD. Chromatographic fingerprints were established to study the variation and discipline of the 3 processed products of Huangqin. PCA and OPLS-DA were used to classify differently processed products of Huangqin. Results: The results showed that baicalin and wogonoside were the main components in the crude and the alcohol Huangqin herb while baicalein and wogonin mainly existed in carbonized Huangqin. The results of mathematical statistics revealed that the processing techniques can make the quality of medicinal materials more uniform. Conclusion: This multivariate monitoring strategy is suitable for quality control in the processing of Huangqin.

DNA Barcode Identification of Black Cohosh Herbal Dietary Supplements

2012 ◽  
Vol 95 (4) ◽  
pp. 1023-1034 ◽  
Author(s):  
David A Baker ◽  
Dennis Wm Stevenson ◽  
Damon P LittLe
Keyword(s):  
Adverse Events ◽  
Dietary Supplements ◽  
Related Species ◽  
Plant Material ◽  
Dna Barcode ◽  
The Other ◽  
Black Cohosh ◽  
Test Plant ◽  
Actaea Racemosa ◽  
Validation Set

Abstract Black cohosh (Actaea racemosa) herbal dietary supplements are commonly consumed to treat menopausal symptoms, but there are reports of adverse events and toxicities associated with their use. Accidental misidentification and/or deliberate adulteration results in harvesting other related species that are then marketed as black cohosh. Some of these species are known to be toxic to humans. We have identified two matK nucleotides that consistently distinguish black cohosh from related species. Using these nucleotides, an assay was able to correctly identify all of the black cohosh samples in the validation set. None of the other Actaea species in the validation set were falsely identified as black cohosh. Of 36 dietary supplements sequenced, 27 (75%) had a sequence that exactly matched black cohosh. The remaining nine samples (25%) had a sequence identical to that of three Asian Actaea species (A. cimicifuga, A. dahurica, and A. simplex). Manufacturers should routinely test plant material using a reliable assay to ensure accurate labeling.

scholarly journals Physiological quality of Gliricidia sepium (Jacq.) Steud. (Leguminosae - Papilionoideae) seeds subjected to different storage conditions

2012 ◽  
Vol 36 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Renata Conduru Ribeiro Reis ◽  
Claudinéia Regina Pelacani ◽  
Cimille Gabrielle Cardoso Antunes ◽  
Bárbara França Dantas ◽  
Renato Delmondez de Castro
Keyword(s):  
Water Content ◽  
Seed Viability ◽  
Gliricidia Sepium ◽  
Storage Conditions ◽  
Physiological Processes ◽  
Plastic Bags ◽  
Starting Point ◽  
Cultivated Plant ◽  
Physiological Quality

Studies of seed physiological processes represent the starting point for the sustainable utilization of native or cultivated plant species from the caatinga biome for which germination studies are still scarce. In order to determine adequate methods for the conservation of Gliricidia sepium seeds, the physiological quality of propagules exposed to different conditions and times of storage were examined. Recently collected seeds of G. sepium were analyzed immediately, while the other sample was stored for three, six, nine, or twelve months in plastic or paper bags under refrigeration or at room temperature for further analysis. The germination rates of seeds from each storage regime were determined and these rates compared to their water content. It was observed that germination capacity was directly related to changes in seed humidity. Storing seeds in plastic bags yielded the best conservation, mainly when they were stored at low temperatures. However, even at temperatures near 25°C it was possible to prolong seed viability when their water content was controlled through the use of impermeable packing.

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