Development of a fast and efficient separation method of secondary metabolites from Rhodiola rosea roots by high performance countercurrent chromatography

AbstractThis study aimed to comprehensively analyze primary and secondary metabolites of three different-colored (white, pale green, and green) pak choi cultivars (Brassica rapa subsp. chinensis) using gas chromatography attached with time-of-flight mass spectrometry (GC-TOFMS) and high-performance liquid chromatography (HPLC). In total, 53 primary metabolites were identified and subjected to partial least-squares discriminant analysis. The result revealed a significant difference in the primary and secondary metabolites between the three pak choi cultivars. In addition, 49 hydrophilic metabolites were detected in different cultivars. Total phenolic and glucosinolate contents were highest in the pale green and green cultivars, respectively, whereas total carotenoid and chlorophyll contents were highest in the white cultivar. Superoxide dismutase activity, 2,2-diphenyl-1-picrylhydraz scavenging, and reducing power were slightly increased in the white, pale green, and green cultivars, respectively. In addition, a negative correlation between pigments and phenylpropanoids was discovered by metabolite correlation analysis. This approach will provide useful information for the development of strategies to enhance the biosynthesis of phenolics, glucosinolates, carotenoids, and chlorophyll, and to improve antioxidant activity in pak choi cultivars. In addition, this study supports the use of HPLC and GC-TOFMS-based metabolite profiling to explore differences in pak choi cultivars.

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Cytokinin Mediated Increased In Vitro Production of Secondary Metabolites with Special Reference to Solasodine in Solanum erianthum

Planta Medica International Open ◽

10.1055/a-1484-9750 ◽

2021 ◽

Vol 8 (02) ◽

pp. e62-e68

Author(s):

Jeeta Sarkar ◽

Nirmalya Banerjee

Keyword(s):

Secondary Metabolites ◽

High Performance ◽

Culture Medium ◽

In Vitro Production ◽

Leaf Extracts ◽

Plant Parts ◽

Regenerated Plants ◽

Vitro Production ◽

Regenerated Plantlets

AbstractSteroid alkaloid solasodine is a nitrogen analogue of diosgenin and has great importance in the production of steroidal medicines. Solanum erianthum D. Don (Solanaceae) is a good source of solasodine. The aim of this study was to evaluate the effect of different cytokinins on the production of secondary metabolites, especially solasodine in the in vitro culture of S. erianthum. For solasodine estimation, field-grown plant parts and in vitro tissues were extracted thrice and subjected to high-performance liquid Chromatography. Quantitative analysis of different secondary metabolites showed that the amount was higher in the in vitro regenerated plantlets compared to callus and field-grown plants. The present study critically evaluates the effect of the type of cytokinin used in the culture medium on solasodine accumulation in regenerated plants. The highest solasodine content (46.78±3.23 mg g-1) was recorded in leaf extracts of the in vitro grown plantlets in the presence of 6-γ,γ-dimethylallylamino purine in the culture medium and the content was 3.8-fold higher compared to the mother plant.

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A general method for the separation of triphenylphosphine oxide and reaction products using high performance countercurrent chromatography

Journal of Chromatography A ◽

10.1016/j.chroma.2013.10.089 ◽

2014 ◽

Vol 1323 ◽

pp. 49-56 ◽

Cited By ~ 4

Author(s):

Neil A. Edwards ◽

Gail Fisher ◽

Guy H. Harris ◽

Nikki Kellichan

Keyword(s):

High Performance ◽

Triphenylphosphine Oxide ◽

Countercurrent Chromatography ◽

Reaction Products ◽

General Method

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Identification of tuliposide G, a novel glucoside ester-type tuliposide, and its distribution in tulip

Zeitschrift für Naturforschung C ◽

10.1515/znc-2019-0176 ◽

2020 ◽

Vol 75 (3-4) ◽

pp. 75-86

Author(s):

Taiji Nomura ◽

Yasuo Kato

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Secondary Metabolites ◽

High Performance ◽

Enzymatic Degradation ◽

Degradation Products ◽

Tulipa Gesneriana ◽

Tissue Extracts ◽

Spectroscopic Analyses ◽

Chemotaxonomic Marker

AbstractTuliposides (Pos) are major defensive secondary metabolites in tulip (genus Tulipa), having 4-hydroxy-2-methylenebutanoyl and/or (3S)-3,4-dihydroxy-2-methylenebutanoyl groups at the C-1 and/or C-6 positions of d-glucose. The acyl group at the C-6 position is converted to antimicrobial lactones, tulipalins, by tuliposide-converting enzymes (TCEs). In the course of a survey of tulip tissue extracts to identify novel Pos, we found a minute high-performance liquid chromatography peak that disappeared following the action of a TCE, and whose retention time differed from those of known Pos. Spectroscopic analyses of the purified compound, as well as its enzymatic degradation products, revealed its structure as 5″-O-(6-O-(4′-hydroxy-2′-methylenebutanoyl))-β-d-glucopyranosyl-(2″R)-2″-hydroxymethyl-4″-butyrolactone, which is a novel glucoside ester-type Pos. We gave this compound the trivial name ‘tuliposide G’ (PosG). PosG accumulated in bulbs, at markedly lower levels than 6-PosA (the major Pos in bulbs), but was not found in any other tissues. Quantification of PosG in bulbs of 52 types of tulip, including 30 cultivars (Tulipa gesneriana) and 22 wild Tulipa spp., resulted in the detection of PosG in 28 cultivars, while PosG was present only in three wild species belonging to the subgenus Tulipa, the same subgenus to which tulip cultivars belong, suggesting the potential usefulness of PosG as a chemotaxonomic marker in tulip.

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