Curcumin Promotes Collagen Type I, Keratinocyte Growth Factor-1, and Epidermal Growth Factor Receptor Expressions in the In Vitro Wound Healing Model of Human Gingival Fibroblasts

European Journal of Dentistry ◽

10.1055/s-0040-1715781 ◽

2020 ◽

Author(s):

Auspreeya Rujirachotiwat ◽

Supaporn Suttamanatwong

Keyword(s):

Gene Expression ◽

Wound Healing ◽

Growth Factor ◽

Keratinocyte Growth Factor ◽

Growth Factor Receptor ◽

Collagen Type I ◽

Type I ◽

Wound Healing Model ◽

Healing Model

Abstract Objective Curcumin promotes oral wound healing; however, the underlying mechanism remains unknown. We hypothesized that curcumin may regulate gene expression in human gingival fibroblasts (hGFs). This study investigated the effect of curcumin on the expression of wound healing–related genes, collagen type I (COL1), keratinocyte growth factor (KGF)-1, and epidermal growth factor receptor (EGFR), in the in vitro wound healing model of hGFs, as well as the signaling pathway involved in the regulation of these genes by curcumin. Materials and Methods The hGFs were treated with curcumin in the unwounded condition and in the in vitro wound healing model (scratch assay). Gene expression was determined by quantitative polymerase chain reaction. PD98059 was used to elucidate whether extracellular signal regulated kinase (ERK) signaling is involved in the curcumin-regulated gene expression in hGFs. Cell migration was also analyzed by the scratch assay. Statistical Analysis Data were analyzed by independent t-test or one-way analysis of variance (ANOVA) followed by Tukey’s Honestly Significant Difference ( HSD) test. Results In unwounded hGFs, curcumin significantly increased KGF-1 and EGFR expressions but not COL1 mRNA expression. Interestingly, curcumin significantly upregulated COL1, KGF-1, and EGFR expressions in the in vitro wound healing model. Furthermore, PD98059 significantly decreased the curcumin-induced COL1 and EGFR expressions, but did not significantly affect KGF-1 upregulation by curcumin. However, hGF migration was not affected by curcumin treatment. Conclusion Curcumin induced KGF-1 and EGFR expressions in unwounded hGFs. In the in vitro wound healing model, curcumin upregulated COL1 and EGFR expression via the ERK pathway and increased KGF-1 expression, possibly by an ERK-independent mechanism.

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Curcumin upregulates transforming growth factor-β1, its receptors, and vascular endothelial growth factor expressions in an in vitro human gingival fibroblast wound healing model

BMC Oral Health ◽

10.1186/s12903-021-01890-9 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Auspreeya Rujirachotiwat ◽

Supaporn Suttamanatwong

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Transforming Growth Factor ◽

Vegf Expression ◽

Wound Healing Model ◽

Β Receptor ◽

Endothelial Growth Factor ◽

Healing Model ◽

Tgf Β1

Abstract Background Curcumin accelerates healing of oral wounds; however, the responsible mechanisms remain underexplored. Our hypothesis is curcumin regulates the expression of wound healing-related genes in human gingival fibroblasts (hGFs). This study investigated whether curcumin regulates transforming growth factor (TGF)-β1, type I TGF-β receptor (TGF-βRI), type II TGF-β receptor (TGF-βRII), and vascular endothelial growth factor (VEGF) expression in unwounded hGFs and an in vitro hGF wound healing model. Methods The cytotoxicity of curcumin was evaluated using the MTT assay. Unwounded hGFs were treated with non-cytotoxic concentrations of curcumin for 24 h. Gene expression was determined by quantitative polymerase chain reaction. Then, hGFs were treated with 1 µM curcumin in an in vitro wound healing model. PD98059 pretreatment was performed to determine whether extracellular signal-regulated kinase (ERK) signaling was required for regulation of gene expression by curcumin. Results Curcumin at 0.1–20 µM caused no significant change in cell viability. In unwounded hGFs, curcumin had no significant effect on TGF-β1, TGF-βRI, TGF-βRII, or VEGF expression. Conversely, curcumin significantly upregulated the expression of these genes in the in vitro wound healing model. PD98059 significantly attenuated the curcumin-stimulated TGF-βRI, TGF-βRII, and VEGF expression, whereas it had no effect on TGF-β1 expression. Conclusions Curcumin upregulated TGF-β1, TGF-βRI, TGF-βRII, and VEGF expression in an in vitro hGF wound healing model. The ERK pathway is required for TGF-βRI, TGF-βRII, and VEGF induction by curcumin. Our findings support the development of curcumin as a therapeutic agent for gingival ulcers.

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Anlotininb as a Promising Inhibitor on Tumor Growth of Oral Squamous Cell Carcinoma Through Cell Apoptosis and Mitotic Catastrophe

10.21203/rs.3.rs-80678/v1 ◽

2020 ◽

Author(s):

Zhaoming Deng ◽

Wei Liao ◽

Wei Wei ◽

Guihua Zhong ◽

Chao He ◽

...

Keyword(s):

Gene Expression ◽

Cell Cycle ◽

Growth Factor ◽

Oral Cancer ◽

Cell Apoptosis ◽

Antitumor Effect ◽

Growth Factor Receptor ◽

Gene Expression Omnibus ◽

Mitotic Catastrophe

Abstract BackgroundOral squamous cell carcinoma (OSCC) has been one of the most malignant cancers in head and neck region. Anlotinib is a tyrosine kinase inhibitor targeting several receptors such as vascular endothelial growth factor receptor (VEGFR), fibroblast growth factor receptor (FGFR), platelet-derived growth factor receptor (PDGFR) and c-Kit. Here we investigated whether Anlotinib have any antitumor effect on oral cancer and tried to explore and explain the possible mechanism.MethodsData from The Cancer Genome Atlas and the Gene Expression Omnibus and Gene Expression Omnibus database was collected to analyze the relationship between the expression of vascular epithelial growth factor receptor 2 and the overall survival rate of OSCC. Oral cancer cell lines Cal-27 and SCC-25 were cultured to conduct all the experiments. In vitro experiments such as CCK-8, colony formation, cell cycle assay and cell apoptosis assay were conducted to detect cell proliferation ability and the change of cell phase and apoptosis. Proteins concerning cell cycle and cell apoptosis were visualized via western blot. α-Tubulin were visualized via immunofluorescence to detect cells undergoing mitotic catastrophe. ResultsHigher expression of VEGFR-2 was significantly related to poorer prognosis. Experiment in vitro demonstrated that cell proliferation was significantly inhibited(p<0.05) after Anlotinib administration and G2/M arrest and apoptosis were both detected in both cell lines. Cycle-related proteins promoting cell cycle progression and proteins related to cell survival were downregulated in Anlotinib group compared to the control group. Cell-death-related biomarker and phosphorylated histone 3 were upregulated in expression in Anlotinib group. Abnormal spindle apparatus was observed in cells undergoing mitotic catastrophe. ConclusionAnlotinib could exert an antitumor effect on oral cancer cells lines via apoptotic pathway and mitotic catastrophe pattern, presenting a promising potential therapy for patients with OSCC.

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Comparative assessment of pulsed electromagnetic fields (PEMF) and pulsed radio frequency energy (PRFE) on an in vitro wound healing model

International Journal of Applied Electromagnetics and Mechanics ◽

10.3233/jae-170129 ◽

2018 ◽

Vol 57 (4) ◽

pp. 427-437

Author(s):

Ozan Karaman ◽

Mehmet Gümüşay ◽

Emine Afra Demirci ◽

Adnan Kaya

Keyword(s):

Wound Healing ◽

Radio Frequency ◽

Electromagnetic Fields ◽

Comparative Assessment ◽

Pulsed Electromagnetic Fields ◽

Radio Frequency Energy ◽

Wound Healing Model ◽

Pulsed Electromagnetic ◽

Healing Model

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Investigation of the effects of prostaglandin E2 on equine superficial digital flexor tendon fibroblasts in vitro

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.3415/vcot-10-03-0044 ◽

2010 ◽

Vol 23 (06) ◽

pp. 417-423 ◽

Cited By ~ 4

Author(s):

J. M. Cissell ◽

S. C. Milton ◽

L. A. Dahlgren

Keyword(s):

Gene Expression ◽

Matrix Protein ◽

Collagen Type ◽

Flexor Tendon ◽

Cell Metabolism ◽

Collagen Type I ◽

Type I ◽

Matrix Metalloproteinase 1 ◽

Superficial Digital Flexor Tendon

Summary Objectives: To evaluate the effects of pros-taglandin E2 (PGE2) treatment on the metabolism of equine tendon fibroblasts in vitro to aid in investigating the response of tendon fibroblasts to injury and novel therapeutics. Methods: Superficial digital flexor tendon fibroblasts isolated via collagenase digestion from six young adult horses were grown in monolayer in four concentrations of PGE2 (0, 10, 50, 100 ng/ml) for 48 hours. Cells and medium were harvested for gene expression (collagen types I and III, cartilage oligomeric matrix protein [COMP], decorin, and matrix metalloproteinase-1, –3, and –13), biochemical analysis (glycosaminoglycan, DNA, and collagen content), and cytological staining. Results: Gene expression for collagen type I was significantly increased at 100 ng/ml PGE2 compared to 10 and 50 ng/ml. There were not any significant differences detected for gene expression of collagen type III, COMP or dec-orin or for biochemical content and cell morphology. Clinical significance: Under the conditions investigated, exogenous treatment of equine tendon fibroblasts with PGE2 failed to alter cell metabolism in a manner useful as a model of tendon injury. A model that applies cyclic strain to a three dimensional construct seeded with tendon fibroblasts may prove to be a more useful model and merits further investigation for this purpose. The ability to assess cellular responses in an environment where the cells are supported within the extracellular matrix may prove beneficial.

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Loliolide Presents Antiapoptosis and Antiscratching Effects in Human Keratinocytes

International Journal of Molecular Sciences ◽

10.3390/ijms20030651 ◽

2019 ◽

Vol 20 (3) ◽

pp. 651 ◽

Cited By ~ 5

Author(s):

Sang Park ◽

Dong Kim ◽

Sunggyu Kim ◽

Laura Lorz ◽

Eunju Choi ◽

...

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Signaling Pathway ◽

Keratinocyte Growth Factor ◽

Growth Factor Receptor ◽

Ethanol Extract ◽

Human Keratinocytes ◽

Freshwater Algae ◽

And Migration ◽

Induced Apoptosis

Loliolide is a monoterpenoid hydroxylactone present in freshwater algae that has anti-inflammatory and antiaging activity; however, its effects on ultraviolet-damaged skin have yet to be elucidated. This study investigated the antiapoptosis and wound-healing effects of loliolide using HaCaT cells (a human keratinocyte cell line). Loliolide inhibited the expression of reactive oxygen species (ROS) induced by ultraviolet radiation as well as wrinkle formation-related matrix metalloproteinase genes and increased the expression of the damage repair-related gene SIRT1. The apoptosis signaling pathway was confirmed by Western blot analysis, which showed that loliolide was able to reduce the expression of caspases 3, 8, and 9, which are related to ROS-induced apoptosis. In addition, Western blotting, reverse-transcription polymerase chain reaction (PCR), and real-time PCR analyses showed that loliolide enhanced the expression of the epidermal growth factor receptor signaling pathway (PI3K, AKT) and migration factors, such as K6, K16, and K17; keratinocyte growth factor; and inflammatory cytokines, such as interleukin (IL)-1, IL-17, and IL-22 expressed during the cellular scratching process, suggesting a putative wound-healing ability. Because of the antiapoptosis and antiscratching effects on skin of both loliolide and loliolide-rich Prasiola japonica ethanol extract, we consider the former to be an important compound used in the cosmeceutical industry.

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Collagen Type I Containing Hybrid Hydrogel Enhances Cardiomyocyte Maturation in a 3D Cardiac Model

Polymers ◽

10.3390/polym11040687 ◽

2019 ◽

Vol 11 (4) ◽

pp. 687 ◽

Cited By ~ 4

Author(s):

Sam G. Edalat ◽

Yongjun Jang ◽

Jongseong Kim ◽

Yongdoo Park

Keyword(s):

Growth Factor ◽

Collagen Type ◽

Transforming Growth Factor ◽

Embryonic Stem ◽

Collagen Type I ◽

Transforming Growth Factor Β1 ◽

Type I ◽

Hybrid Hydrogel ◽

Cardiac Model

In vitro maturation of cardiomyocytes in 3D is essential for the development of viable cardiac models for therapeutic and developmental studies. The method by which cardiomyocytes undergoes maturation has significant implications for understanding cardiomyocytes biology. The regulation of the extracellular matrix (ECM) by changing the composition and stiffness is quintessential for engineering a suitable environment for cardiomyocytes maturation. In this paper, we demonstrate that collagen type I, a component of the ECM, plays a crucial role in the maturation of cardiomyocytes. To this end, embryonic stem-cell derived cardiomyocytes were incorporated into Matrigel-based hydrogels with varying collagen type I concentrations of 0 mg, 3 mg, and 6 mg. Each hydrogel was analyzed by measuring the degree of stiffness, the expression levels of MLC2v, TBX18, and pre-miR-21, and the size of the hydrogels. It was shown that among the hydrogel variants, the Matrigel-based hydrogel with 3 mg of collagen type I facilitates cardiomyocyte maturation by increasing MLC2v expression. The treatment of transforming growth factor β1 (TGF-β1) or fibroblast growth factor 4 (FGF-4) on the hydrogels further enhanced the MLC2v expression and thereby cardiomyocyte maturation.

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