Evaluation of the Interference of Hemoglobin Variant J-Bangkok on Glycated Hemoglobin (HbA1c) Measurement by Five Different Methods

2017 ◽  
Vol 125 (10) ◽  
pp. 655-660
Author(s):  
Dong-Mei Wen ◽  
Sheng-Nan Xu ◽  
Wei-Jia Wang ◽  
Xiu-Ming Zhang ◽  
Ming-Huan Suo ◽  
...  
Keyword(s):  
High Performance ◽  
Glycated Hemoglobin ◽  
Clinical Laboratory ◽  
Hemoglobin Variant ◽  
Deming Regression ◽  
Significant Difference ◽  
The Difference ◽  
Immuno Assay ◽  
Hba1c Measurement ◽  
Significant Interference

Abstract Objective The interference of the hemoglobin variant (Hb J-Bangkok) was evaluated on 4 different glycated hemoglobin assays and compared with a reference immuno assay. Methods An overall test of coincidence of 2 least-squares linear regression lines was performed to determine whether the presence of Hb J-Bangkok caused a statistically significant difference in HbA1c results compared with a reference immuno assay. Statistical analysis was performed on the difference of the estimated average glucose calculated from HbA1c values and fasting plasma glucose in the Hb J-Bangkok variant group using the different detection systems. Deming regression analysis was used to determinate whether Hb J-Bangkok had a significant interference on HbA1c results using an HbA1c±10% relative bias at 6% and 9% HbA1c as evaluation limits. Results Turbidimetric inhibition immunoassay method, and enzymatic methods were not affected by Hb J-Bangkok. However, Hb J-Bangkok showed statistically significant interference to the two ion-exchange high-performance liquid chromatography methods. Conclusion When performing HbA1c tests, clinical laboratory personnel should identify the Hb variant and select the appropriate methods or use alternative indicators.

scholarly journals Effects of Hemoglobin (Hb) E and HbD Traits on Measurements of Glycated Hb (HbA1c) by 23 Methods

2008 ◽  
Vol 54 (8) ◽  
pp. 1277-1282 ◽  
Author(s):  
Randie R Little ◽  
Curt L Rohlfing ◽  
Steve Hanson ◽  
Shawn Connolly ◽  
Trefor Higgins ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Comparative Method ◽  
Deming Regression ◽  
Significant Difference ◽  
Patients With Diabetes ◽  
Ion Exchange Hplc ◽  
Clinically Significant ◽  
Hba1c Measurement ◽  
Significant Interference

Abstract Background: Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbD are the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measurement in the presence of HbE and/or HbD traits. Methods: We evaluated 23 HbA1c methods; 9 were immunoassay methods, 10 were ion-exchange HPLC methods, and 4 were capillary electrophoresis, affinity chromatography, or enzymatic methods. An overall test of coincidence of 2 least-squares linear regression lines was performed to determine whether the presence of HbE or HbD traits caused a statistically significant difference from HbAA results relative to the boronate affinity HPLC comparative method. Deming regression analysis was performed to determine whether the presence of these traits produced a clinically significant effect on HbA1c results with the use of ±10% relative bias at 6% and 9% HbA1c as evaluation limits. Results: Statistically significant differences were found in more than half of the methods tested. Only 22% and 13% showed clinically significant interference for HbE and HbD traits, respectively. Conclusions: Some current HbA1c methods show clinically significant interferences with samples containing HbE or HbD traits. To avoid reporting of inaccurate results, ion-exchange chromatograms must be carefully examined to identify possible interference from these Hb variants. For some methods, manufacturers’ instructions do not provide adequate information for making correct decisions about reporting results.

scholarly journals Voxelotor Treatment Interferes With Quantitative and Qualitative Hemoglobin Variant Analysis in Multiple Sickle Cell Disease Genotypes

2020 ◽  
Vol 154 (5) ◽  
pp. 627-634
Author(s):  
Nicola J Rutherford-Parker ◽  
Sean T Campbell ◽  
Jennifer M Colby ◽  
Zahra Shajani-Yi
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
High Performance ◽  
Clinical Laboratory ◽  
The United States ◽  
Cell Disease ◽  
Hemoglobin Variant ◽  
Hbd Punjab ◽  
The Impact

Abstract Objectives Voxelotor was recently approved for use in the United States as a treatment for sickle cell disease (SCD) and has been shown to interfere with the quantitation of hemoglobin (Hb) S percentage. This study aimed to determine the effect of voxelotor on the quantitation of hemoglobin variant levels in patients with multiple SCD genotypes. Methods In vitro experiments were performed to assess the impact of voxelotor treatment on hemoglobin variant testing. Whole blood samples were incubated with voxelotor and then analyzed by routinely used quantitative and qualitative clinical laboratory methods (high-performance liquid chromatography [HPLC], capillary zone electrophoresis [CZE], and acid and alkaline electrophoresis). Results Voxelotor modified the α-globin chain of multiple hemoglobins, including HbA, HbS, HbC, HbD-Punjab, HbE, HbA2, and HbF. These voxelotor-hemoglobin complexes prevented accurate quantitation of multiple hemoglobin species, including HbS, by HPLC and CZE. Conclusions Technical limitations in quantifying HbS percentage may preclude the use of HPLC or CZE for monitoring patients treated with voxelotor. Furthermore, it is unclear whether HbS-voxelotor complexes are clinically equivalent to HbS. Consensus guidelines for reporting hemoglobin variant percentages for patients taking voxelotor are needed, as these values are necessary for determining the number of RBC units to exchange in acute situations.

Liquid-chromatographic determination of serotonin in serum and plasma.

1978 ◽  
Vol 24 (9) ◽  
pp. 1509-1514 ◽  
Author(s):  
S Sasa ◽  
C L Blank ◽  
D C Wenke ◽  
C A Sczupak
Keyword(s):  
High Performance ◽  
Clinical Laboratory ◽  
Chromatographic Determination ◽  
Present Scheme ◽  
Long Term Storage ◽  
Significant Difference ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Using high-performance liquid chromatography with electrochemical detection, we determined serotonin in plasma from parkinsonian patients being treated with L-3,4-dihydroxyphenylalanine or N-(DL-seryl)-N'(2,3,4-trihydroxybenzyl)hydrochloride plus L-3,4-dihydroxyphenylalanine ("Sinemet") and in serum from a blood bank, from "normal" persons, and a pooled specimen from a hospital clinical laboratory. The values obtained for the two groups of Parkinson's disease patients showed no significant difference. Long-term storage on solid CO2 was xhown to be an adequate technique for preserving samples. The mean (+/-SEAM) normal value obtained for serotonin in serum was 146 +/- 46 microgram/liter (n = 23), a result in harmony with that previously obtained [Clin. Chem. 20, 812 (1974)] by fluorometry. In comparison to other methods for measurement of serotonin in serum or plasma, we believe that the present scheme offers greater selectivity, sensitivity, and precision.

scholarly journals Analysis of incapacitation during +Gz induced loss of consciousness

2021 ◽  
Vol 65 ◽  
pp. 91-96
Author(s):  
MD Sharma ◽  
P Biswal ◽  
N Taneja ◽  
A Agarwal
Keyword(s):  
Air Force ◽  
High Performance ◽  
Age Groups ◽  
Rapid Onset ◽  
Loss Of Consciousness ◽  
Video Recordings ◽  
Operational Capabilities ◽  
Significant Difference ◽  
The Mean ◽  
The Difference

Introduction: Occurrence of G induced loss of consciousness (G-LOC) during centrifuge training is a known entity. The Indian Air Force is currently undertaking high G training of its fighter pilots in High Performance Human Centrifuge (HPHC) which has significantly higher operational capabilities. The study aimed to analyse the incapacitation periods and myoclonic jerks associated with G-LOC occurring during HPHC training. Material and Methods: Records of episodes of 161 G-LOC during closed loop Rapid Onset Rates (ROR) runs over a period of 7 years from 2011 to 2017 were analyzed. The video recordings of these G-LOC episodes were assessed in terms of the participant demographics, +Gz onset rates, incapacitation periods, and presence or absence of myoclonic jerks during the G-LOC episodes. Descriptive statistics was applied to analyze the incapacitation periods and the duration of the myoclonic flail movements. Single tailed t-test was used to analyze the difference between the incapacitation periods of the aircrew who suffered myoclonic flail movements and those who did not. One-way ANOVA was carried out to assess the differences in incapacitation periods, if any, between aircrew of different age groups. Significance was set at P < 0.05. Results: Of these 161 episodes of G-LOC, 43.5% were seen in trainee aircrew. The mean Absolute Incapacitation Period (AIP), Relative Incapacitation Period (RIP) and Total Incapacitation Period (TIP) was 6.9 ± 2.3 s, 12.2 ± 4.7 s, and 19.1 ± 5.5 respectively. Age and flying experience did not show any significant effect with any of the incapacitation periods (p>0.05). The TIP correlated better with the RIP than with the AIP (Pearson’s correlation values of 0.9 and 0.52 respectively). Myoclonic flail movements were witnessed in 25.5% of G-LOC episodes with an average duration of 5.3 s and more than 50% occurring at ≥8Gz. The mean duration of RIP was significantly higher (P=0.03) when G-LOC was associated with myoclonic flail movements, whereas, the duration of AIP did not show any significant difference. Conclusion: The shorter incapacitation periods observed in the study compared to that reported in most of the previous studies could be attributed to the faster Gz offset rates of the present HPHC. Episodes of G-LOC having myoclonic movements were found to have higher relative incapacitation periods. Even though these movements were associated with G-LOC occurring at higher Gz levels, the durations were independent of the Gz levels.

scholarly journals Establishment of PIVKA-Ⅱ Reference Intervals From Hospital-stored Data: A Comparison Analysis

2021 ◽  
Author(s):  
Lewei Zhou ◽  
Qiyuan Su ◽  
Yan Yao ◽  
Meixian Xiang ◽  
Jiesheng Zhen ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Reference Intervals ◽  
Female Group ◽  
Comparison Analysis ◽  
Test Items ◽  
Health Group ◽  
Significant Difference ◽  
The Difference ◽  
Gender Specific ◽  
Indirect Reference

Abstract Objective The authors aimed to explore methods to establish indirect reference intervals for PIVKA-II from hospital-stored data.Method 7623 patient specimens of the Renmin Hospital of Wuhan University were collected. Indirect reference intervals were established based on the hospital-stored data with four different methods, including the Hoffmann method (HM), revised Hoffmann method (HMCDF), E-M algorithm-based method (EMBCT), and a recent estimator (KOSMIC). According to CLSI C28-A3 guidelines, 369 healthy specimens were collected. The authors tested the difference between reference intervals of gender-specific and age-specific subgroups using Harris and Boyd's test. Finally, the averaging result of estimates was calculated according to how likely each model was.Results The indirect reference intervals of PIVKA-II based on LIS data were 0 to 35.30 mAU/mL (HM), 0 to 31.48 mAU/mL (HMCDF), 0 to 30.78 mAU/mL (EMBCT), 0 to 36.17 mAU/mL (KOSMIC) and 0 to 31.48 mAU/mL (averaging) respectively, and the reference intervals based on healthy group were 0 to 32 mAU/mL. Compared with HM, EMBCT and KOSMIC, HMCDF and the averaging result was closer to those of the health group. Significant difference was detected between gender-partitioned subgroups, and the reference upper limit in the female group was smaller than the male group.Conclusions The authors established the indirect reference intervals of PIVKA-II for the Wuhan population, which could be used to the clinical reference intervals. The framework proposed could help clinical laboratory set their reference intervals of test items.

scholarly journals Pharmacokinetics and pharmacodynamics of a novel amoxicillin/sulbactam/prednisolone intramammary infusion in lactating cows after repeated administrations

2014 ◽  
Vol 59 (No. 5) ◽  
pp. 221-229 ◽  
Author(s):  
H. Li ◽  
GQ Wu ◽  
SS Tang ◽  
XL Xiao ◽  
JC Li
Keyword(s):  
High Performance ◽  
Similar Efficacy ◽  
Duration Of Action ◽  
Lactating Cows ◽  
Significant Difference ◽  
Liquid Chromatography Tandem Mass ◽  
Repeated Administrations ◽  
Tandem Mass Spectrometric ◽  
The Difference ◽  
High Concentrations

The aim of this study was to assess the pharmacokinetics (PK) and pharmacodynamics (PD) of a novel anti-mastitis preparation, amoxicillin/sulbactam/prednisolone intramammary infusion (CAIMM), containing 200 mg amoxicillin, 50 mg sulbactam and 10 mg prednisolone per 3 g formulation, in healthy lactating cows after repeated administrations. A parallel study was performed using the available combination product (Synulox<sup>&reg;</sup> LC) from Pfizer, with the aim of comparing the two formulations. The concentrations of drugs in quarter milk were determined using the ultra-high performance liquid chromatography tandem mass spectrometric (UPLC-MS/MS) method. No significant difference in the major PK parameters (C<sub>max</sub>, T<sub>max</sub>, MRT, t<sub>1/2&lambda;</sub>, and AUC<sub>last</sub>) was observed. The MIC<sub>90</sub> determined in 106 isolated Staphylococcus spp., 64 Streptococcus spp. and 18 Escherichia coli strains was 0.5, 0.25 and 2 &mu;g/ml, respectively. The PK/PD evaluation showed that the effective duration of action (t &gt; MIC<sub>90</sub>) for CAIMM (42 &plusmn; 2.46 h) was increased by 0.86 times compared with Synulox<sup>&reg;</sup> LC (34 &plusmn; 3.17&nbsp;h), but the difference was not significant (P &gt; 0.05). This pharmacokinetic and pharmacodynamic study revealed that CAIMM maintained high concentrations in quarter milk for the three ingredients after repeated intramammary administrations and a similar efficacy was achieved with Synulox<sup>&reg;</sup> LC. &nbsp;

scholarly journals Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Vani Chandrashekar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Rank Correlation ◽  
Beta Thalassemia ◽  
Compound Heterozygous ◽  
Hb A1c ◽  
Spearman’S Rank Correlation ◽  
Significant Difference ◽  
The Difference

Hb A1c measurement is subject to interference by hemoglobin traits and this is dependent on the method used for determination. In this paper we studied the difference between Hb A1c measured by HPLC in hemoglobin traits and normal chromatograms. We also studied the correlation of Hb A1c with age. Hemoglobin analysis was carried out by high performance liquid chromatography. Spearman’s rank correlation was used to study correlation between A1c levels and age. Mann-WhitneyUtest was used to study the difference in Hb A1c between patients with normal hemoglobin and hemoglobin traits. A total of 431 patients were studied. There was positive correlation with age in patients with normal chromatograms only. No correlation was seen in Hb E trait or beta thalassemia trait. No significant difference in Hb A1c of patients with normal chromatograms and patients with hemoglobin traits was seen. There is no interference by abnormal hemoglobin in the detection of A1c by high performance liquid chromatography. This method cannot be used for detection of A1c in compound heterozygous and homozygous disorders.

Natural Occurrence of Fusarium Species, Fumonisin Production by Toxigenic Strains, and Concentrations of Fumonisins B1 and B2 in Conventional and Organic Maize Grown in Spain

2007 ◽  
Vol 70 (1) ◽  
pp. 151-156 ◽  
Author(s):  
AGUSTÍN ARIÑO ◽  
TERESA JUAN ◽  
GLORIA ESTOPAÑAN ◽  
JOSÉ F. GONZÁLEZ-CABO
Keyword(s):  
High Performance ◽  
Fusarium Species ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Farming System ◽  
Natural Occurrence ◽  
Significant Difference ◽  
The Difference ◽  
Organic Farming System ◽  
Fumonisins B1 And B2

Sixty samples of corn from both conventional and organic farms were tested for internal fungal contamination. Molds were identified to genus, and those belonging to the genus Fusarium were identified to species. Twenty isolates of Fusarium verticillioides were tested with a high-performance liquid chromatography–naphthalene dicarboxaldehyde–fluorescence method for their ability to produce fumonisins B1 and B2. The internal fungal infection in organic maize (63.20%) was significantly higher than that in conventional maize (40.27%) (P &lt; 0.05). However, the distribution of fungal genera indicated a significantly higher prevalence of Fusarium in conventional (34.93%) than in organic (18.15%) maize, making Fusarium the predominant fungus in conventional maize. This difference in mold distribution between organic and conventional maize was attributed to the difference in cultivation system. The dominant Fusarium species in both conventional and organic samples was F. verticillioides. There were no significant differences in the ability of 20 selected isolates of F. verticillioides to produce fumonisins on conventional or organic corn. Up to 13.3% of the conventional corn samples contained fumonisins B1 and B2 at mean concentrations of 43 and 22 ng/g, respectively. Organic corn samples had somewhat lower levels of contamination: 35 ng/g fumonisin B1 and 19 ng/g fumonisin B2 (P &gt; 0.05). The organic farming system, with well-balanced crop rotation, tillage, and compost fertilization, produced corn that was less likely to be contaminated with Fusarium species, although no significant difference in fumonisin concentrations was found between the two types of contaminated corn.

scholarly journals Microfinance and Performance of Micro and Small Enterprises: Does Training have an Impact

2017 ◽  
Vol 4 (1) ◽  
pp. 1 ◽  
Author(s):  
Syed Hussain Haider ◽  
Muzaffar Asad ◽  
Minaa Fatima ◽  
Rana Zain Ul Abidin
Keyword(s):  
Performance Indicators ◽  
High Performance ◽  
Small Enterprises ◽  
Access To Finance ◽  
Policy Makers ◽  
The People ◽  
Significant Difference ◽  
Micro And Small Enterprises ◽  
The Difference ◽  
And Performance

Access to finance is considered as very important for gaining high performance of Micro and Small Enterprises (MSEs). Yet it has been observed that despite the availability of access to finance the performance of several MSEs is poor, which is a major hurdle in the growth rate of MSEs. The purpose of this study was to find the difference in certain performance indicators of MSEs who owners have been given training against those whose owners have never been given any kind of training. In order to conduct study survey research has been conducted and a sample of 384 MSEs was selected on simple random basis. The findings revealed that all the performance indicators including sales increase, income increase, assets increase, employment increase, and meeting household expenses have shown a significant difference among the two groups. The findings of the study are very important for the policy makers and the people who are involved in microfinance industry.

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