scholarly journals Identification of Bioactive Compounds in Polar and Nonpolar Extracts of Araujia sericifera

2017 ◽  
Vol 4 (03) ◽  
pp. e93-e103
Author(s):  
Martina Palomino-Schätzlein ◽  
Mary Montaño ◽  
Pablo Escrig ◽  
Herminio Boira ◽  
Avelino Corma ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Moderate Activity ◽  
Human Colon Carcinoma Cell ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Breast Carcinoma Cell Lines ◽  
Conduritol F

Abstract Araujia sericifera is a native perennial, climbing laticiferous shrub from South America that is currently naturalized in many other countries. Previous data describe promising properties for A. sericifera, but no systematic study of its bioactive compounds and possible medicinal applications has been conducted to date. In the present study, aerial parts of A. sericifera (leaves, stems, and fruits) were explored by combining GC-MS and NMR spectroscopy analysis for both nonpolar (hexane) and polar (methanol) extracts. The hexanic extracts contained high amounts of pentacyclic triterpenes including two new metabolites, 3-tigloyl germanicol (18) and 3-tigloyl lupeol (19). The methanolic extracts revealed the presence of luteolin-7-glucoside (24), trigonelline (22), and conduritol F (23) as the main constituents. A multivariate study of a meaningful number of extracts allowed us to determine the distribution of compounds inside the plant. A cytotoxic evaluation in vitro showed that both leaf and fruit hexanic extracts presented a moderate activity against human breast carcinoma cell lines (MDA-MB-453 and MCF-7) and human colon carcinoma cell line (HCT-116) by the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay.

scholarly journals The Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus

2021 ◽  
Author(s):  
Ameneh Shokati ◽  
Masoud Soleimani ◽  
Saeid Abroun
Keyword(s):  
Colorectal Cancer ◽  
Lactobacillus Acidophilus ◽  
Carcinoma Cell ◽  
Lactobacillus Rhamnosus ◽  
Human Colon ◽  
Human Colon Carcinoma Cell ◽  
Colon Carcinoma Cell Line ◽  
Human Colon Carcinoma ◽  
Caco2 Cells

Abstract Background: The epidemiological studies indicated that colorectal cancer is one of the most common types of cancer in the world and is considered a leading cause of cancer-related death. The present study aimed to investigate the inhibitory effect of lactobacillus acidophilus supernatant (LAS) and lactobacillus rhamnosus supernatant (LRS) on the growth and invasiveness of the human colon carcinoma cell line (Caco2) in-vitro. Methods: In this experimental study, the anti-proliferative activity and anti-invasion potential of LAS and LRS were determined by MTT and transwell chambers assays, respectively. The expression of mitochondrial membrane potential-9 (MMP-9) and matrix metalloproteinase-12 (MMP12) genes were analyzed by real-time PCR.Results: The results indicated that supernatants of these two lactobacilli had cytotoxic effects on Caco-2 cells at a concentration of 25% v/v and higher. Thus, the minimum concentrations (25% V/V) of supernatants were chosen for further experiments. LAS and LRS could significantly suppress the invasiveness of Caco-2 cells. Also, the expression of MMP12 was significantly increased in Caco-2 cells when treated with LAS, whereas LRS had no significant effect on the invasive capacity and the gene expression levels of MMP12. The expression of MMP-9 was statistically decreased in Caco2 cells treated with LAS and LRS (P<0.00001).Conclusion: In general, it was shown that LAS and LRS exert anti-cancer activity against the growth, invasion, and metastasis of Caco2 cells in-vitro. It seems that these two bacteria could be used as prophylactic and therapeutic agents for the prevention and treatment of colorectal cancer.

scholarly journals Synthesis, Spectral Characterization, andIn VitroCytotoxicity of N-2′-Hydroxyethyl-Substituted Azacholestanes Prepared from 6-Oxocholestanes by Modified Schmidt Reaction

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Shahab A. A. Nami ◽  
Suraiya Khan ◽  
Mahboob Alam ◽  
M. Mushfiq ◽  
Dong-Ung Lee ◽  
...  
Keyword(s):  
Cell Line ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Spectroscopic Characterization ◽  
Carcinoma Cell Line ◽  
High Yield ◽  
Spectroscopic Techniques ◽  
Human Colon Carcinoma Cell ◽  
Schmidt Reaction

The present paper reports the synthesis and spectroscopic characterization of few N-2′-hydroxyethyl-substituted azacholestanes using BF3-OEt2, TiCl4, SnCl4, and H2SO4as catalysts in moderate yields by a modified version of Schmidt reaction. A notable feature is the passivity of SnCl4in case of 3β-acetoxy-N-2′-hydroxyethyl-6-aza-B-homo-5α-cholestan-7-one and 3β-chloro-N-2′-hydroxyethyl-6-aza-B-homo-5α-cholestan-7-one. However, the reaction was unsuccessful in case of N-2′-Hydroxyethyl-6-aza-B-homo-5α-cholestan-7-one. Another striking aspect is the attainment of high yield in case of H2SO4as catalyst. The semisolid compounds are characterized using various spectroscopic techniques such as FT-IR,1H-NMR and mass spectra, and microanalytical data. A reaction mechanism has been proposed on the basis of previous studies. Moreover, the compounds have also been screened for theirin vitrocytotoxicity against human colon carcinoma cell line, HCT116, and human liver hepatocellular carcinoma cell line, HepG2, using doxorubicin as standard. On the basis of IC50values, 3β-chloro-N-2′-hydroxyethyl-6-aza-B-homo-5α-cholestan-7-one (5) was found to inhibit the cancer cells most effectively.

The use of fractal analysis to describe in vitro drug effects: Effect of lovastatin on CaCo-2 cell differentiation

1994 ◽  
Vol 52 ◽  
pp. 352-353
Author(s):  
Beverly E. Maleeff ◽  
Timothy K. Hart ◽  
Anne M. Klinkner ◽  
C. Robbie Waites ◽  
Peter J. Bugelski ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Carcinoma Cell ◽  
Drug Effects ◽  
Human Colon ◽  
Human Colon Carcinoma Cell ◽  
Polycarbonate Membrane ◽  
Cell Height ◽  
Human Colon Carcinoma ◽  
Inhibit Cell Growth

Inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoAR) have been shown to inhibit cell growth and to affect cell differentiation in a variety of in vitro systems. The human colon carcinoma cell line CaCo-2, when grown on permeable membranes, undergoes high order differentiation into a well-organized monolayer of columnar enterocytes. However, in the presence of the HMG CoAR inhibitor lovastatin, these cells fail to differentiate, lose contact inhibition and overgrow the culture support. Changes in cell differentiation can be described by measurements of mean cell height (MCH) or mean monolayer height (MMH), and are illustrated with representative micrographs. In this study, we have applied fractal geometry in addition to traditional morphometry to quantitatively describe the effect of lovastatin on CaCo-2 cell differentiation in vitro.CaCo-2 cells were plated at sub-confluency (∽ 60,000 cells/cm2) on collagen-coated Transwell™ permeable polycarbonate membrane supports (Costar Corp., Cambridge, MA) in culture dishes and allowed to attach for at least 24 hours.

Anticancer effects of amooranin in human colon carcinoma cell line in vitro and in nude mice xenografts

2006 ◽  
Vol 119 (10) ◽  
pp. 2443-2454 ◽  
Author(s):  
Cheppail Ramachandran ◽  
P.K. Raveendran Nair ◽  
Arturo Alamo ◽  
Curtis Bruce Cochrane ◽  
Enrique Escalon ◽  
...  
Keyword(s):  
Cell Line ◽  
Colon Carcinoma ◽  
Nude Mice ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Human Colon Carcinoma Cell ◽  
Colon Carcinoma Cell Line ◽  
Anticancer Effects ◽  
Human Colon Carcinoma

scholarly journals The alpha v beta 6 integrin promotes proliferation of colon carcinoma cells through a unique region of the beta 6 cytoplasmic domain.

1994 ◽  
Vol 127 (2) ◽  
pp. 547-556 ◽  
Author(s):  
M Agrez ◽  
A Chen ◽  
R I Cone ◽  
R Pytela ◽  
D Sheppard
Keyword(s):  
Amino Acid ◽  
Colon Carcinoma ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Cytoplasmic Domain ◽  
Human Colon Carcinoma Cell ◽  
Unique Region ◽  
Fibronectin Receptor

Cell-matrix interactions are assumed to be important in regulating differentiation and tumor cell growth; however, the precise roles of individual matrix receptors in producing cellular responses are still unclear. We have previously described the alpha v beta 6 integrin, an epithelial cell fibronectin receptor expressed in many carcinoma cell lines. Here we show that heterologous expression of alpha v beta 6 in a human colon carcinoma cell line (SW480) enhances the proliferative capacity of these cells, both in vitro and in vivo in nude mice. This property of alpha v beta 6 correlates with the presence of an 11-amino acid region at the COOH terminus of the beta 6 cytoplasmic domain. This 11-amino acid sequence is required for the growth stimulatory effect, but not for other functions of the beta 6 cytoplasmic domain, such as promoting cell adhesion and focal contact localization.

In Vitro chemosensitivity test of SK-302B on human colon carcinoma cell lines

1996 ◽  
Vol 19 (4) ◽  
pp. 261-263 ◽  
Author(s):  
Soo Kie Kim ◽  
Chan Mug Ahn ◽  
Tae-Ue Kim ◽  
Sun-Ju Choi ◽  
Yoon Sun Park ◽  
...  
Keyword(s):  
Colon Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Chemosensitivity Test ◽  
Human Colon Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Human Colon Carcinoma ◽  
In Vitro Chemosensitivity Test
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