Cryptographic Hash Algorithms Based on Time Averaging Techniques

2007 ◽  
Author(s):  
Jurate Ragulskiene ◽  
Algimantas Fedaravicius ◽  
Minvydas Ragulskis ◽  
Theodore E. Simos ◽  
George Psihoyios ◽  
...  
2006 ◽  
Vol 35 (3) ◽  
Author(s):  
Loreta Saunorienė ◽  
Minvydas Ragulskis

Time averaging interferometry is an experimental technique widely applied in different areas of optical engineering. Typical example is an application of time averaging techniques for the analysis of fringes produced by a moiré grating formed on the surface of oscillating elastic structure. Unfortunately, time averaged moiré produces blurred images. Therefore, special digital image filtering is to be used for highlighting the pattern of time averaged fringes. Such a procedure is essential for the investigation of dynamical systems with non-harmonic motion as the produced moiré pattern is particularly blurred. This paper proposes some techniques for the sharpening of fringes in time averaged moiré patterns.


Author(s):  
Kathleen M. Marr ◽  
Mary K. Lyon

Photosystem II (PSII) is different from all other reaction centers in that it splits water to evolve oxygen and hydrogen ions. This unique ability to evolve oxygen is partly due to three oxygen evolving polypeptides (OEPs) associated with the PSII complex. Freeze etching on grana derived insideout membranes revealed that the OEPs contribute to the observed tetrameric nature of the PSIl particle; when the OEPs are removed, a distinct dimer emerges. Thus, the surface of the PSII complex changes dramatically upon removal of these polypeptides. The atomic force microscope (AFM) is ideal for examining surface topography. The instrument provides a topographical view of individual PSII complexes, giving relatively high resolution three-dimensional information without image averaging techniques. In addition, the use of a fluid cell allows a biologically active sample to be maintained under fully hydrated and physiologically buffered conditions. The OEPs associated with PSII may be sequentially removed, thereby changing the surface of the complex by one polypeptide at a time.


Author(s):  
M. Unser ◽  
B.L. Trus ◽  
A.C. Steven

Since the resolution-limiting factor in electron microscopy of biological macromolecules is not instrumental, but is rather the preservation of structure, operational definitions of resolution have to be based on the mutual consistency of a set of like images. The traditional measure of resolution for crystalline specimens in terms of the extent of periodic reflections in their diffraction patterns is such a criterion. With the advent of correlation averaging techniques for lattice rectification and the analysis of non-crystalline specimens, a more general - and desirably, closely compatible - resolution criterion is needed. Two measures of resolution for correlation-averaged images have been described, namely the differential phase residual (DPR) and the Fourier ring correlation (FRC). However, the values that they give for resolution often differ substantially. Furthermore, neither method relates in a straightforward way to the long-standing resolution criterion for crystalline specimens.


2018 ◽  
Author(s):  
Kristopher M. Kusnerik ◽  
◽  
Harley Means ◽  
Roger W. Portell ◽  
Michal Kowalewski

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 314 ◽  
Author(s):  
Tânia D. Tavares ◽  
Joana C. Antunes ◽  
Jorge Padrão ◽  
Ana I. Ribeiro ◽  
Andrea Zille ◽  
...  

The increased resistance of bacteria against conventional pharmaceutical solutions, the antibiotics, has raised serious health concerns. This has stimulated interest in the development of bio-based therapeutics with limited resistance, namely, essential oils (EOs) or antimicrobial peptides (AMPs). This study envisaged the evaluation of the antimicrobial efficacy of selected biomolecules, namely LL37, pexiganan, tea tree oil (TTO), cinnamon leaf oil (CLO) and niaouli oil (NO), against four bacteria commonly associated to nosocomial infections: Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa. The antibiotic vancomycin and silver nanoparticles (AgNPs) were used as control compounds for comparison purposes. The biomolecules were initially screened for their antibacterial efficacy using the agar-diffusion test, followed by the determination of minimal inhibitory concentrations (MICs), kill-time kinetics and the evaluation of the cell morphology upon 24 h exposure. All agents were effective against the selected bacteria. Interestingly, the AgNPs required a higher concentration (4000–1250 μg/mL) to induce the same effects as the AMPs (500–7.8 μg/mL) or EOs (365.2–19.7 μg/mL). Pexiganan and CLO were the most effective biomolecules, requiring lower concentrations to kill both Gram-positive and Gram-negative bacteria (62.5–7.8 μg/mL and 39.3–19.7 μg/mL, respectively), within a short period of time (averaging 2 h 15 min for all bacteria). Most biomolecules apparently disrupted the bacteria membrane stability due to the observed cell morphology deformation and by effecting on the intracellular space. AMPs were observed to induce morphological deformations and cellular content release, while EOs were seen to split and completely envelope bacteria. Data unraveled more of the potential of these new biomolecules as replacements for the conventional antibiotics and allowed us to take a step forward in the understanding of their mechanisms of action against infection-related bacteria.


Atmosphere ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 645
Author(s):  
Kristen Okorn ◽  
Michael Hannigan

As low-cost sensors have become ubiquitous in air quality measurements, there is a need for more efficient calibration and quantification practices. Here, we deploy stationary low-cost monitors in Colorado and Southern California near oil and gas facilities, focusing our analysis on methane and ozone concentration measurement using metal oxide sensors. In comparing different sensor signal normalization techniques, we propose a z-scoring standardization approach to normalize all sensor signals, making our calibration results more easily transferable among sensor packages. We also attempt several different physical co-location schemes, and explore several calibration models in which only one sensor system needs to be co-located with a reference instrument, and can be used to calibrate the rest of the fleet of sensor systems. This approach greatly reduces the time and effort involved in field normalization without compromising goodness of fit of the calibration model to a significant extent. We also explore other factors affecting the performance of the sensor system quantification method, including the use of different reference instruments, duration of co-location, time averaging, transferability between different physical environments, and the age of metal oxide sensors. Our focus on methane and stationary monitors, in addition to the z-scoring standardization approach, has broad applications in low-cost sensor calibration and utility.


2021 ◽  
Vol 75 (1) ◽  
pp. 39-70
Author(s):  
Lorna J. Smith ◽  
Wilfred F. van Gunsteren ◽  
Bartosz Stankiewicz ◽  
Niels Hansen

AbstractValues of 3J-couplings as obtained from NMR experiments on proteins cannot easily be used to determine protein structure due to the difficulty of accounting for the high sensitivity of intermediate 3J-coupling values (4–8 Hz) to the averaging period that must cover the conformational variability of the torsional angle related to the 3J-coupling, and due to the difficulty of handling the multiple-valued character of the inverse Karplus relation between torsional angle and 3J-coupling. Both problems can be solved by using 3J-coupling time-averaging local-elevation restraining MD simulation. Application to the protein hen egg white lysozyme using 213 backbone and side-chain 3J-coupling restraints shows that a conformational ensemble compatible with the experimental data can be obtained using this technique, and that accounting for averaging and the ability of the algorithm to escape from local minima for the torsional angle induced by the Karplus relation, are essential for a comprehensive use of 3J-coupling data in protein structure determination.


Author(s):  
Z. C. Ong ◽  
C. C. Lee

A novel modal analysis technique called impact-synchronous modal analysis (ISMA) was introduced in previous research. With the utilization of impact-synchronous time averaging (ISTA), this modal analysis can be performed in presence of ambient forces whereas the conventional analysis method requires machines to be totally shut down. However, lack of information of phase angles with respect to impact in ISMA has caused it to be labor-intensive and time-consuming. An automated impact device (AID) is introduced in this study in the effort to replace the manually operated impact hammer and prepare it to be used in the current practice of ISMA on the purpose of enhancing its effectiveness and practicability. Impact profile and isolation effect are noted to be the contributing parameters in this study. This paper devoted on calibrating and controlling of the AID which gives the desired impact profiles as compared to the manual impact hammer. The AID is found effective in the determination of dynamic characteristics when the device is isolated from the boundary condition of the test structure.


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