scholarly journals Antibiotic susceptibility test based on the dielectrophoretic behavior of elongated Escherichia coli with cephalexin treatment

2011 ◽  
Vol 5 (2) ◽  
pp. 021102 ◽  
Author(s):  
Cheng-Che Chung ◽  
I-Fang Cheng ◽  
Wen-Horng Yang ◽  
Hsien-Chang Chang
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Antibiotic Susceptibility Test

scholarly journals Antimicrobial Susceptibility Pattern of Uropathogenic Bacteria in RMMC Hospital of Chidambaram

2019 ◽  
Vol 9 (4-A) ◽  
pp. 334-339
Author(s):  
A. Gnanasekaran ◽  
P. Manikandan ◽  
J. Vigneshwari ◽  
P. Poongothai ◽  
P. K. Senthilkumar
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Urine Samples ◽  
Resistance Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Antibiotic Susceptibility Test ◽  
Life Threatening ◽  
Uropathogenic Bacteria

Background: In every year millions of people were affected by the Urinary Tract Infection. It was creating a serious health issue. Aim: The present study was to analysis of the uropathogenic bacteria in patients were attended RMMC Hospital and their antibiotic resistance pattern, in vitro detection of haemolysis virulent factor of uropathogenic. Material and Methods: All urine samples were tested by the standard microbiological procedure. Kirby-Bauer method used for the Antibiotic Susceptibility Test according to the CLSI guidelines. Commercially available antibiotics were used. Blood Agar used for the detection of haemolysis. Results: A total of 261 urine samples were included in this study. We isolated a total of 103 positive cultures. 12% of Gram-positive, 83% of Gram-negative bacteria and 3% of Candida fungi. Escherichia coli was the most predominant bacteria (54%) followed by Klebsiella sp (15%), Staphylococcus aureus (12%), Pseudomonas aeruginosa (12%), Proteus (1%) and fungi Candida (3%). Mostly female patients’ sample were analysed and the inpatient higher majority than the outpatients. Conclusion: Escherichia coli are the common bacteria to cause of UTI. Nowadays most of the uropathogens are to resistance to the overall antibiotics. This kind of reactions creating the life-threatening of humans. Keywords: Antibiotic, Antibiotic Susceptibility Test, Uropathogens, Resistance, Haemolysis

scholarly journals Bloodstream infections initiated by ESBL-producing Escherichia coli in neonates and infants at two hospitals in Yaoundé, Cameroon

2021 ◽  
Vol 15 (3) ◽  
pp. 1222-1233
Author(s):  
Carole Suzie Lacmago Titsamp ◽  
Hortense Kamga Gonsu ◽  
Simon Ngamli Fewou
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Bloodstream Infection ◽  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Blood Cultures ◽  
Susceptibility Test ◽  
Coagulase Negative Staphylococci ◽  
Antibiotic Susceptibility Test ◽  
Neonates And Infants

Early diagnosis and probabilistic antibiotic therapy based on known bacterial ecology and antibiotic sensibility can reduce mortality and morbidity in pathologies caused by a bacterial infection. This study aimed at determining the prevalence and risk factors of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli isolated from blood cultures of neonates and infants population. We conducted a cross-sectional study during which pathogenic bloodstream isolates were identified. Antibiotic susceptibility test was performed on Escherichia coli isolates and phenotypic confirmation of ESBL production by Escherichia coli was performed by a double-disc synergy test. Over the course of this study, 298 blood cultures were performed and 129 (43.3%) positive cultures were obtained. Of the 129 bacterial isolates, 90 (69.7%) were Escherichia coli and 39 (30.2%) were other bacteria strains that included Klebsiella oxytoca, Streptococcus pneumonia, and Coagulase-negative staphylococci. Antibiotic susceptibility test indicated that Escherichia coli isolates were resistant to cephalosporin, penicillin, sulfonamide, and aminoglycoside antibiotic families. Further analysis indicated that 31 (34.4%) Escherichia coli strains were ESBL producers and risk factors for bloodstream infection by ESBL-producing Escherichia coli were prior to exposure to antibiotics and immune system depression. These findings clearly extend our understanding of the type of resistant initiated by ESBL-producing Escherichia coli in bloodstream infection of neonates, and infants and also provides useful information that can guide the establishment of an efficient therapeutic strategy for the community- and hospital-acquired bloodstream infection.

scholarly journals Antibiotic Resistance Profile of Escherichia coli and Klebsiella spp Isolated from Post-weaned Piglet Faeces under Penicillin-streptomycin Combination Therapy (Penstrep®) in Abidjan, Côte d’Ivoire

2019 ◽  
pp. 1-10
Author(s):  
A. N. T. Koné ◽  
N. K. N’gbesso ◽  
B. K. Guédé ◽  
M. B. Ouattara ◽  
F. K. Konan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Combination Therapy ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Weaned Piglets ◽  
Klebsiella Species ◽  
Antibiotic Resistance Profile ◽  
Antibiotic Susceptibility Test ◽  
Klebsiella Spp

Aim: The current study was aimed at evaluating the antibiotic resistance profile of Escherichia coli and Klebsiella spp isolated from post-weaned piglets treated with penicillin-streptomycin combination therapy. Study Design:  Bacteriological study. Place and Duration of Study: Laboratory of the National Reference Center for antibiotics at Institut Pasteur Côte d’Ivoire, between March 2018 and June 2018. Methodology: Escherichia coli and Klebsiella species were isolated from post weaned piglets stool on Mac Conkey medium added up separately with penicillin, streptomycin and combined penicillin-streptomycin and identified. Antibiotic susceptibility test was performed using disk diffusion method on Müller-Hinton agar. Results: Escherichia coli and Klebsiella species resistance to penicillin, streptomycin and combined penicillin-streptomycin evaluated, respectively, reached 80.5% (Day 0) to 92.0% (Day 4); 17.0% (Day 0) to 39.9% (Day 4) and 31.3% (Day 0) to 70.9% (Day 4) for piglets treated with Penstrep®. In addition, antibiotic susceptibility test carried out for Escherichia coli and Klebsiella spp strains isolated from piglets treated with Penstrep® revealed resistance to amoxicillin for each bacterial species at a rate of 86.0% and 89.0%. Furthermore, control piglets showed lesser resistance to streptomycin (E.coli 31.0% and Klebsiella spp 38.0%) than those treated with the combination therapy Penstrep® (E. coli 73.0% and Klebsiella spp 48.0%). As for netilmicin, imipenem and colistin, no resistance was detected for treated piglets as well as untreated ones. Conclusion: The combination therapy with Penstrep® has increased Escherichia coli and Klebsiella species resistance to antibiotics tested in the current study.

scholarly journals Enterococci used as complementary indicator of fecal contamination to assess water quality from public schools in the city of Curitiba, Paraná, Brazil

2020 ◽  
Vol 23 ◽  
Author(s):  
Mariana Amabile Waideman ◽  
Vivian Plaça Teixeira ◽  
Elisa Hizuru Uemura ◽  
Tânia Montenegro Stamford ◽  
Diego Averaldo Guiguet Leal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Public Schools ◽  
Antibiotic Susceptibility ◽  
Fecal Contamination ◽  
Susceptibility Test ◽  
Antibiotic Susceptibility Test ◽  
The City ◽  
Microbiological Indicators

Abstract The study evaluated the drinking water quality from 45 public schools in the city of Curitiba, Brazil. Water samples were harvested from two different points at each school: the first one, drinking fountain used by students (n=45) and the second, kitchen faucet (n=45), comprising 90 samples. The samples were submitted to analysis according to current legislation, such as: dosage of free chlorine; colony counts of heterotrophic bacteria; presence of total coliform bacteria and Escherichia coli. In addition, an enterococci analysis was also included as a complementary microbiological indicator of fecal contamination. The isolated strains of enterococci were identified and submitted to an antibiotic susceptibility test. In relation to the legal parameters, 35.5% (n=16) of the 45 schools evaluated did not meet the quality water standards set by the Brazilian legislation. Although none of the 90 samples exhibited the presence Escherichia coli, 6.6% (n=6) of the analyzed samples presented enterococci contamination. Regarding the antibiotic susceptibility test, the isolated strains demonstrated a similar behavior, became resistant to six antibiotics and had intermediate sensitivity to one (Streptomycin) of the nine antibiotics tested. The drinking water destined for human consumption in the public schools may pose health risks for students since the fecal contamination by enterococci was confirmed, and it could be observed that the water may be a source of genes from bacteria resistant to antimicrobials. This study reinforces the importance of monitoring complementary microbiological indicators to assure water quality, seeing that fecal contamination was detected only when alternative parameters were inserted, emphasizing the relevance of including microbiological indicators beyond what the legislation requires to evaluate the drinking water more effectively and to safeguard public health.

scholarly journals Bio-activities of Tetracycline and Algae Food Supplement Algo-Bio® on Escherichia coli Antimicrobial Resistance Isolated from Piglet’s Intestinal Flora

2019 ◽  
pp. 1-8
Author(s):  
Amine Naty Tadiogo Kone ◽  
Baguy Mohamed Ouattara ◽  
Bertin Tiekoura ◽  
Fernique Kouadio Konan ◽  
Eugène Koffi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Antibiotic Susceptibility ◽  
Intestinal Flora ◽  
Susceptibility Test ◽  
Diffusion Method ◽  
Bacteriological Study ◽  
Antibiotic Susceptibility Test ◽  
Resistance Rates ◽  
Study Laboratory

Aims: This study aimed at evaluating the effect of Algo-Bio® use on Escherichia coli resistance strains isolated from piglets intestinal flora. Study Design: Bacteriological study. Place and Duration of Study: Laboratory of the National Reference Center for antibiotics at Institute Pasteur Côte d’Ivoire, between March 2018 and June 2018. Methodology: A breeding of three batches of two piglets was carried out, then treatments with tetracycline and Algo-Bio® were administered to them. Enterobacteria was isolated on Mac Conkey medium added up with tetracycline and resistance rates were determined. Escherichia coli resistant strains have been identified and antibiotic susceptibility test was performed using disk diffusion method on Müller-Hinton agar. Results: Enterobacteria resistance rates increased ranging from 18.4% (D0) to 81.5% (D4) to tetracycline-treated piglets and respectively from 25.7% (D0) to 29% (D4) and from 22.3% (D0) to 24.5% (D4), in control piglets and those treated with Algo-Bio®. Antibiotic susceptibility test of Escherichia coli strains isolated from piglets treated with tetracycline showed high resistance to ceftazidime (83.3%), amoxicillin (76.9%) and tetracycline (92.3%) with 39.4% strains producing ESBL, 23.7% producing PHL and 5.2% of producing CHP on D4. Escherichia coli strains isolated from control piglets and Algo-Bio® treated piglets revealed a decrease of ESBL respectively from 17.6% (D0) to 13.7% (D4) and from 12.5% ​​(D0) to 6.4% (D4). Conclusion: The study showed that the use of Algo-Bio® does not induce an evolution of antimicrobial resistance in Microbiota strains and consequently this dietary supplement can be used as a good alternative to antibiotics.

Epidemiology, risk factors and antimicrobial resistance of Escherichia coli bacteremia

2021 ◽  
Vol 11 (Number 2) ◽  
pp. 58-67
Author(s):  
Mahjuba Umme Salam ◽  
Selina Yasmin ◽  
Md. Rashedul Haque ◽  
Sharmin Ahmed ◽  
Shahidul Alam ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Antibiotic Susceptibility ◽  
Antimicrobial Agents ◽  
Blood Stream Infection ◽  
Incidence Rates ◽  
Diffusion Method ◽  
Antibiotic Susceptibility Test ◽  
Multiple Drugs

Background: Escherichia coli is a common causative of blood stream infection having potentials to produce significant morbidity and mortality. This organism also has the ability to develop resistance against antimicrobial agents. Knowing its epidemiology, risk factors and antimicrobial resistance patterns can help preventing and managing bacteremia caused by this organism. Materials and methods: This was across sectional observational study carried out from February 2017 to February 2018 on 64 blood culture positive Escherichia coli infected patients admitted in Medicine inpatient of a medical college hospital. Age, sex, mode of acquisition of infection, history of prior empiric antibiotic treatment, duration of hospital stay, development of complication were observed and noted. Antibiotic susceptibility test for all isolates was performed by Kirby-Bauer disc diffusion method. Predesigned semi-structured data collection from was used and collected data were analyzed manually and expressed in descriptive statistical terms. Results: Of the 64 enrolled patients, 47(73.43%) were female. Average age of affection was 53.48±20.65 years and increased incidence rates (51.56%) was observed at age >60 years. Infection was communityacquired in 35.84% cases and urinary tract infection was the most frequent (46.3) risk factor. More than eighty seven percent of samples showed resistance to at least one antimicrobial agent and resistance to multiple drugs was associated with complications. Conclusion: Escherichia coli bacteremia has high incidence rates for antimicrobial resistance and mortality. Continuous surveillance and antibiotic susceptibility pattern monitoring is essential to develop regional antibiotic therapy protocols.

scholarly journals Microdilution Antibiotic Susceptibility Test: Examination of Certain Variables

1973 ◽  
Vol 26 (5) ◽  
pp. 658-665 ◽  
Author(s):  
R. C. Tilton ◽  
L. Lieberman ◽  
E. H. Gerlach
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Antibiotic Susceptibility Test

scholarly journals Improve antibiotic susceptibility test and its quality control by linear-gradient plate

2017 ◽  
Vol 47 (7) ◽  
pp. 740-749
Author(s):  
Qing ZHANG ◽  
Yin ZHANG ◽  
Jing QI ◽  
YanBo LUO ◽  
LuLu LI ◽  
...  
Keyword(s):  
Quality Control ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Linear Gradient ◽  
Antibiotic Susceptibility Test ◽  
Gradient Plate
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