scholarly journals Enterococci used as complementary indicator of fecal contamination to assess water quality from public schools in the city of Curitiba, Paraná, Brazil

2020 ◽  
Vol 23 ◽  
Author(s):  
Mariana Amabile Waideman ◽  
Vivian Plaça Teixeira ◽  
Elisa Hizuru Uemura ◽  
Tânia Montenegro Stamford ◽  
Diego Averaldo Guiguet Leal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Public Schools ◽  
Antibiotic Susceptibility ◽  
Fecal Contamination ◽  
Susceptibility Test ◽  
Antibiotic Susceptibility Test ◽  
The City ◽  
Microbiological Indicators

Abstract The study evaluated the drinking water quality from 45 public schools in the city of Curitiba, Brazil. Water samples were harvested from two different points at each school: the first one, drinking fountain used by students (n=45) and the second, kitchen faucet (n=45), comprising 90 samples. The samples were submitted to analysis according to current legislation, such as: dosage of free chlorine; colony counts of heterotrophic bacteria; presence of total coliform bacteria and Escherichia coli. In addition, an enterococci analysis was also included as a complementary microbiological indicator of fecal contamination. The isolated strains of enterococci were identified and submitted to an antibiotic susceptibility test. In relation to the legal parameters, 35.5% (n=16) of the 45 schools evaluated did not meet the quality water standards set by the Brazilian legislation. Although none of the 90 samples exhibited the presence Escherichia coli, 6.6% (n=6) of the analyzed samples presented enterococci contamination. Regarding the antibiotic susceptibility test, the isolated strains demonstrated a similar behavior, became resistant to six antibiotics and had intermediate sensitivity to one (Streptomycin) of the nine antibiotics tested. The drinking water destined for human consumption in the public schools may pose health risks for students since the fecal contamination by enterococci was confirmed, and it could be observed that the water may be a source of genes from bacteria resistant to antimicrobials. This study reinforces the importance of monitoring complementary microbiological indicators to assure water quality, seeing that fecal contamination was detected only when alternative parameters were inserted, emphasizing the relevance of including microbiological indicators beyond what the legislation requires to evaluate the drinking water more effectively and to safeguard public health.

scholarly journals The rationale for introducing the indices «Generalized coliform bacteria» and «Escherichia coli» into the scheme of sanitary and microbiological control of water quality as indices of fecal contamination

2021 ◽  
Vol 99 (12) ◽  
pp. 1353-1359
Author(s):  
Angelika V. Zagainova ◽  
Galina M. Trukhina ◽  
Yury A. Rakhmanin ◽  
Tamara Z. Artemova ◽  
Marina A. Sukhina
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Fecal Contamination ◽  
Coliform Bacteria ◽  
E Coli ◽  
Intestinal Infections ◽  
Safety Of Drinking Water ◽  
Microbiological Indicators

Introduction. The increasing bacterial contamination of water bodies requires an increase in water quality control’s reliability to ensure epidemic safety against waterborne infections. Therefore, researchers in both Russia and Europe came to the conclusion that it is necessary to search for indicator microorganisms that can more accurately suggest the presence of pathogens. microorganisms in water than traditional indicators. The aim of the study was to justify the introduction of indicator indices of fecal contamination “generalized coliform bacteria” and Escherichia coli to assess the safety of drinking water Material and methods. The article provides an analysis of domestic and international regulatory documents and literary materials regulating the quality of drinking water in terms of sanitary and microbiological indicators and assessment criteria. The results of many years of experimental and field research carried out by research organizations and practical organizations of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare are presented. Results. On the territory of Russia, “general coliform bacteria,” is one of the indicator indices chosen according to the fermentation of lactose, determine the safety of drinking water. The water does not take into account pathogenic bacteria (Salmonella, Shigella) and a number of lactose-negative opportunistic bacteria, causative agents of intestinal infections. The study of microorganisms isolated from the feces of patients confirms the frequency of occurrence of lactose-negative microorganisms to varying from 20 to 100% of strains. With an annual trend towards a decrease in the percentage of non-standard drinking water samples in terms of microbiological indices, general intestinal infections (GII) of unknown etiology increase, i.e. risk of GII. If the quality of drinking water does not correspond to thermotolerant coliform bacteria (TCB), 95% of samples contain E. coli. Therefore, the determination of E. coli more reliably indicates the intake of fresh fecal contamination and provides efficiency in taking measures to eliminate an unfavorable situation than TCB. Conclusion. Reasons are given for the introduction of more reliable microbiological indicators of water safety control, such as - “generalized coliform bacteria” with the preservation of the abbreviation GCB, combining both lactose-positive and lactose-negative bacteria, determined by the sign of glucose fermentation, negative oxidase test and negative stain according to Gram and E. coli as an indicator of recent faecal contamination, which will allow the assessment of water quality for a wide range of bacteria of the order Enterobacterials, corresponding to the modern taxonomy of Enterobacteriaceae NCBI, will ensure harmonization with international requirements and the safety of drinking water for the population.

scholarly journals Escherichia coli (E. coli) as an Indicator of Fecal Contamination in Water: A Review

2019 ◽  
Author(s):  
Farhan Mohammad Khan ◽  
Rajiv Gupta
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Fecal Coliform ◽  
Fecal Contamination ◽  
Waterborne Diseases ◽  
Fecal Matter ◽  
E Coli ◽  
Detection Techniques ◽  
Pollution Indicator

Escherichia coli or E. coli is a member of the fecal coliform group and is a more specific indicator of fecal contamination than other fecal coliform species, its presence indicate possibly presence of harmful bacteria which will cause diseases and it also suggests the extent as well as the nature of the contaminants. E. coli bacteria able to survive in water for 4 – 12 weeks and at present, it appears as an indicator to provide the accurate bacterial contamination of fecal matter in drinking water, because of the availability of simple, affordable, fast, sensitive and exact detection techniques. According to the laboratory experiment based techniques, 24 - 48 hours are required for the bacterial concentration to be reported. So, there is a necessity for continuous monitoring. Techniques for detection of many pathogenic bacterial strains are not yet available, sometimes days to weeks are required to get the results. To overcome the difficulties, expensive and time-consuming techniques are required to detect, count and identify the presence of specific bacterial strain. Public health relies on online monitoring of water quality that depends majorly on examination of fecal indicator bacteria, thus protection of health requires fecal pollution indicator so that it is not required to analyze drinking water to overcome the problems associated with waterborne diseases. This paper will brief the classification, sources, survival of E. coli bacteria and its correlation with basic water quality parameters in water sources.```

scholarly journals Antimicrobial Susceptibility Pattern of Uropathogenic Bacteria in RMMC Hospital of Chidambaram

2019 ◽  
Vol 9 (4-A) ◽  
pp. 334-339
Author(s):  
A. Gnanasekaran ◽  
P. Manikandan ◽  
J. Vigneshwari ◽  
P. Poongothai ◽  
P. K. Senthilkumar
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Urine Samples ◽  
Resistance Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Antibiotic Susceptibility Test ◽  
Life Threatening ◽  
Uropathogenic Bacteria

Background: In every year millions of people were affected by the Urinary Tract Infection. It was creating a serious health issue. Aim: The present study was to analysis of the uropathogenic bacteria in patients were attended RMMC Hospital and their antibiotic resistance pattern, in vitro detection of haemolysis virulent factor of uropathogenic. Material and Methods: All urine samples were tested by the standard microbiological procedure. Kirby-Bauer method used for the Antibiotic Susceptibility Test according to the CLSI guidelines. Commercially available antibiotics were used. Blood Agar used for the detection of haemolysis. Results: A total of 261 urine samples were included in this study. We isolated a total of 103 positive cultures. 12% of Gram-positive, 83% of Gram-negative bacteria and 3% of Candida fungi. Escherichia coli was the most predominant bacteria (54%) followed by Klebsiella sp (15%), Staphylococcus aureus (12%), Pseudomonas aeruginosa (12%), Proteus (1%) and fungi Candida (3%). Mostly female patients’ sample were analysed and the inpatient higher majority than the outpatients. Conclusion: Escherichia coli are the common bacteria to cause of UTI. Nowadays most of the uropathogens are to resistance to the overall antibiotics. This kind of reactions creating the life-threatening of humans. Keywords: Antibiotic, Antibiotic Susceptibility Test, Uropathogens, Resistance, Haemolysis

scholarly journals Bloodstream infections initiated by ESBL-producing Escherichia coli in neonates and infants at two hospitals in Yaoundé, Cameroon

2021 ◽  
Vol 15 (3) ◽  
pp. 1222-1233
Author(s):  
Carole Suzie Lacmago Titsamp ◽  
Hortense Kamga Gonsu ◽  
Simon Ngamli Fewou
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Bloodstream Infection ◽  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Blood Cultures ◽  
Susceptibility Test ◽  
Coagulase Negative Staphylococci ◽  
Antibiotic Susceptibility Test ◽  
Neonates And Infants

Early diagnosis and probabilistic antibiotic therapy based on known bacterial ecology and antibiotic sensibility can reduce mortality and morbidity in pathologies caused by a bacterial infection. This study aimed at determining the prevalence and risk factors of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli isolated from blood cultures of neonates and infants population. We conducted a cross-sectional study during which pathogenic bloodstream isolates were identified. Antibiotic susceptibility test was performed on Escherichia coli isolates and phenotypic confirmation of ESBL production by Escherichia coli was performed by a double-disc synergy test. Over the course of this study, 298 blood cultures were performed and 129 (43.3%) positive cultures were obtained. Of the 129 bacterial isolates, 90 (69.7%) were Escherichia coli and 39 (30.2%) were other bacteria strains that included Klebsiella oxytoca, Streptococcus pneumonia, and Coagulase-negative staphylococci. Antibiotic susceptibility test indicated that Escherichia coli isolates were resistant to cephalosporin, penicillin, sulfonamide, and aminoglycoside antibiotic families. Further analysis indicated that 31 (34.4%) Escherichia coli strains were ESBL producers and risk factors for bloodstream infection by ESBL-producing Escherichia coli were prior to exposure to antibiotics and immune system depression. These findings clearly extend our understanding of the type of resistant initiated by ESBL-producing Escherichia coli in bloodstream infection of neonates, and infants and also provides useful information that can guide the establishment of an efficient therapeutic strategy for the community- and hospital-acquired bloodstream infection.

scholarly journals Contamination of Escherichia coli Drinking Water Refills on Drinking Water Depots in Malang City

2019 ◽  
Vol 7 (1) ◽  
pp. 9
Author(s):  
Wiwik Kusmawati ◽  
Lia Rahayu
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
The City ◽  
Maximum Limit

Data from the Malang City Health Office shows the increasing number of refill drinking water depots in the city of Malang until 2013 there were 163 drinking water depots scattered in each Malang Subdistrict. The purpose of this study was to determine the contamination of Escherichia coli refill drinking water in drinking water depots in Malang. The samples taken were 20 refill drinking water depots in Malang. Sampling is done by simple random sampling. Escherichia coli examination using the Most Probable Number Test. Of the 20 samples tested, 2 samples met the requirements of drinking water quality according to PERMENKES and 18 other samples containing the number of coliform bacteria exceeding the maximum limit of 0 per 100 ml of water. There were Escherichia coli in 10 samples while 8 other samples contained other coliform bacteria.

scholarly journals Antibiotic Resistance Profile of Escherichia coli and Klebsiella spp Isolated from Post-weaned Piglet Faeces under Penicillin-streptomycin Combination Therapy (Penstrep®) in Abidjan, Côte d’Ivoire

2019 ◽  
pp. 1-10
Author(s):  
A. N. T. Koné ◽  
N. K. N’gbesso ◽  
B. K. Guédé ◽  
M. B. Ouattara ◽  
F. K. Konan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Combination Therapy ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Weaned Piglets ◽  
Klebsiella Species ◽  
Antibiotic Resistance Profile ◽  
Antibiotic Susceptibility Test ◽  
Klebsiella Spp

Aim: The current study was aimed at evaluating the antibiotic resistance profile of Escherichia coli and Klebsiella spp isolated from post-weaned piglets treated with penicillin-streptomycin combination therapy. Study Design:  Bacteriological study. Place and Duration of Study: Laboratory of the National Reference Center for antibiotics at Institut Pasteur Côte d’Ivoire, between March 2018 and June 2018. Methodology: Escherichia coli and Klebsiella species were isolated from post weaned piglets stool on Mac Conkey medium added up separately with penicillin, streptomycin and combined penicillin-streptomycin and identified. Antibiotic susceptibility test was performed using disk diffusion method on Müller-Hinton agar. Results: Escherichia coli and Klebsiella species resistance to penicillin, streptomycin and combined penicillin-streptomycin evaluated, respectively, reached 80.5% (Day 0) to 92.0% (Day 4); 17.0% (Day 0) to 39.9% (Day 4) and 31.3% (Day 0) to 70.9% (Day 4) for piglets treated with Penstrep®. In addition, antibiotic susceptibility test carried out for Escherichia coli and Klebsiella spp strains isolated from piglets treated with Penstrep® revealed resistance to amoxicillin for each bacterial species at a rate of 86.0% and 89.0%. Furthermore, control piglets showed lesser resistance to streptomycin (E.coli 31.0% and Klebsiella spp 38.0%) than those treated with the combination therapy Penstrep® (E. coli 73.0% and Klebsiella spp 48.0%). As for netilmicin, imipenem and colistin, no resistance was detected for treated piglets as well as untreated ones. Conclusion: The combination therapy with Penstrep® has increased Escherichia coli and Klebsiella species resistance to antibiotics tested in the current study.

scholarly journals Bio-activities of Tetracycline and Algae Food Supplement Algo-Bio® on Escherichia coli Antimicrobial Resistance Isolated from Piglet’s Intestinal Flora

2019 ◽  
pp. 1-8
Author(s):  
Amine Naty Tadiogo Kone ◽  
Baguy Mohamed Ouattara ◽  
Bertin Tiekoura ◽  
Fernique Kouadio Konan ◽  
Eugène Koffi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Antibiotic Susceptibility ◽  
Intestinal Flora ◽  
Susceptibility Test ◽  
Diffusion Method ◽  
Bacteriological Study ◽  
Antibiotic Susceptibility Test ◽  
Resistance Rates ◽  
Study Laboratory

Aims: This study aimed at evaluating the effect of Algo-Bio® use on Escherichia coli resistance strains isolated from piglets intestinal flora. Study Design: Bacteriological study. Place and Duration of Study: Laboratory of the National Reference Center for antibiotics at Institute Pasteur Côte d’Ivoire, between March 2018 and June 2018. Methodology: A breeding of three batches of two piglets was carried out, then treatments with tetracycline and Algo-Bio® were administered to them. Enterobacteria was isolated on Mac Conkey medium added up with tetracycline and resistance rates were determined. Escherichia coli resistant strains have been identified and antibiotic susceptibility test was performed using disk diffusion method on Müller-Hinton agar. Results: Enterobacteria resistance rates increased ranging from 18.4% (D0) to 81.5% (D4) to tetracycline-treated piglets and respectively from 25.7% (D0) to 29% (D4) and from 22.3% (D0) to 24.5% (D4), in control piglets and those treated with Algo-Bio®. Antibiotic susceptibility test of Escherichia coli strains isolated from piglets treated with tetracycline showed high resistance to ceftazidime (83.3%), amoxicillin (76.9%) and tetracycline (92.3%) with 39.4% strains producing ESBL, 23.7% producing PHL and 5.2% of producing CHP on D4. Escherichia coli strains isolated from control piglets and Algo-Bio® treated piglets revealed a decrease of ESBL respectively from 17.6% (D0) to 13.7% (D4) and from 12.5% ​​(D0) to 6.4% (D4). Conclusion: The study showed that the use of Algo-Bio® does not induce an evolution of antimicrobial resistance in Microbiota strains and consequently this dietary supplement can be used as a good alternative to antibiotics.

Evaluation of Ground Water Quality For AL Shuwayja area –Wasit Provence Using GIS Technique

2017 ◽  
Vol 1 (2) ◽  
pp. 1-11
Author(s):  
Ali Nasser Hilo
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Ground Water ◽  
Water Quality Index ◽  
Quality Index ◽  
Poor Quality ◽  
Quality Parameters ◽  
Ground Water Quality ◽  
Water Index ◽  
The City

The low level of water in rivers in Iraq leads to poor water quality, on that basis; we need to assess Iraq's water resources for uses of irrigation and drinking water. This study present a model accounts for ground water quality by using a water quality index (WQI) for the region defined between the city of Kut and the city of Badra in Wasit province. this study relies on a system of wells set up along the path through the Badra –Kut  and around it  up to 78 wells. The study showed poor quality of ground water in the region of study and it is unsuitability for irrigation and drinking water, as well as provided a solution to the water accumulated in the Shuwayja to reduce the bad effect on groundwater by using a system of branch and collection canals  then pumping at the effluent  of Al  Shuwayja in seasons of rainy season ..Water quality index calculated depend on the basis of various physic-chemical parameters as PH, Ec , TDS, TSS, Nacl , SO4 ,Na , and  Mg. The resultant and analytical are present with use of Arch GIS program – geostastical analysis for the water index and water quality parameters

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